Fluorescence spectroscopy for tongue carcinoma detection: Study in an animal model

Detalhes bibliográficos
Autor(a) principal: Kurachi, C.
Data de Publicação: 2005
Outros Autores: Fontana, C. R., Melo, C. A S, Rosa, L. E B [UNESP], Bagnato, V. S.
Tipo de documento: Artigo de conferência
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1117/12.588222
http://hdl.handle.net/11449/68341
Resumo: Fluorescence diagnosis of malignant lesions has been showed as an attractive optical technique due especially to its real-time response and a more objective and quantitative evaluation. Even though the oral cavity allows a direct examination many lesions are diagnosed when it is already in advanced stage, compromising the patient prognosis. In this study, the fluorescence spectroscopy was used to the detection of chemically induced carcinoma at the lateral border of the tongue in a hamster model. Two excitations wavelengths in visible region were applied: 442 and 532 nm. All the spectra results were analyzed comparing with the histopathological diagnosis. The better results were achieved with the 442 nm laser excitation. The spectra from carcinoma showed new emission bands and these were used to determined different ratios for a quantitative analysis. Using the 625-645 nm fluorescence range under 442 nm excitation (A3 coefficient) the percentage of false negative was of 9.1%, however the false positive percentage was of 18.5%. The 532 nm excitation provided a better normal tissue detection compared to 442 nm excitation. The ideal clinical condition is probably the use of multiple wavelengths excitation for a broader tissue fluorescence investigation.
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spelling Fluorescence spectroscopy for tongue carcinoma detection: Study in an animal modelDiagnosisFluorescenceSpectroscopyTongue carcinomaAlcoholsBiological organsHistologyMetabolismNondestructive examinationPathologyTumorsCancer detectionFluorescene spectroscopyHistopathological diagnosisFluorescence diagnosis of malignant lesions has been showed as an attractive optical technique due especially to its real-time response and a more objective and quantitative evaluation. Even though the oral cavity allows a direct examination many lesions are diagnosed when it is already in advanced stage, compromising the patient prognosis. In this study, the fluorescence spectroscopy was used to the detection of chemically induced carcinoma at the lateral border of the tongue in a hamster model. Two excitations wavelengths in visible region were applied: 442 and 532 nm. All the spectra results were analyzed comparing with the histopathological diagnosis. The better results were achieved with the 442 nm laser excitation. The spectra from carcinoma showed new emission bands and these were used to determined different ratios for a quantitative analysis. Using the 625-645 nm fluorescence range under 442 nm excitation (A3 coefficient) the percentage of false negative was of 9.1%, however the false positive percentage was of 18.5%. The 532 nm excitation provided a better normal tissue detection compared to 442 nm excitation. The ideal clinical condition is probably the use of multiple wavelengths excitation for a broader tissue fluorescence investigation.Institute de Física de São Carlos Universidade de São PauloFaculdade de Odontologia de São José dos Campos Universidade Estadual PaulistaFaculdade de Odontologia de São José dos Campos Universidade Estadual PaulistaUniversidade de São Paulo (USP)Universidade Estadual Paulista (Unesp)Kurachi, C.Fontana, C. R.Melo, C. A SRosa, L. E B [UNESP]Bagnato, V. S.2014-05-27T11:21:23Z2014-05-27T11:21:23Z2005-07-21info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObject210-217http://dx.doi.org/10.1117/12.588222Progress in Biomedical Optics and Imaging - Proceedings of SPIE, v. 5689, p. 210-217.1605-7422http://hdl.handle.net/11449/6834110.1117/12.5882222-s2.0-218444440767595315660858413Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengProgress in Biomedical Optics and Imaging - Proceedings of SPIE0,243info:eu-repo/semantics/openAccess2021-10-23T21:44:20Zoai:repositorio.unesp.br:11449/68341Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T19:27:11.571125Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Fluorescence spectroscopy for tongue carcinoma detection: Study in an animal model
title Fluorescence spectroscopy for tongue carcinoma detection: Study in an animal model
spellingShingle Fluorescence spectroscopy for tongue carcinoma detection: Study in an animal model
Kurachi, C.
Diagnosis
Fluorescence
Spectroscopy
Tongue carcinoma
Alcohols
Biological organs
Histology
Metabolism
Nondestructive examination
Pathology
Tumors
Cancer detection
Fluorescene spectroscopy
Histopathological diagnosis
title_short Fluorescence spectroscopy for tongue carcinoma detection: Study in an animal model
title_full Fluorescence spectroscopy for tongue carcinoma detection: Study in an animal model
title_fullStr Fluorescence spectroscopy for tongue carcinoma detection: Study in an animal model
title_full_unstemmed Fluorescence spectroscopy for tongue carcinoma detection: Study in an animal model
title_sort Fluorescence spectroscopy for tongue carcinoma detection: Study in an animal model
author Kurachi, C.
author_facet Kurachi, C.
Fontana, C. R.
Melo, C. A S
Rosa, L. E B [UNESP]
Bagnato, V. S.
author_role author
author2 Fontana, C. R.
Melo, C. A S
Rosa, L. E B [UNESP]
Bagnato, V. S.
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade de São Paulo (USP)
Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Kurachi, C.
Fontana, C. R.
Melo, C. A S
Rosa, L. E B [UNESP]
Bagnato, V. S.
dc.subject.por.fl_str_mv Diagnosis
Fluorescence
Spectroscopy
Tongue carcinoma
Alcohols
Biological organs
Histology
Metabolism
Nondestructive examination
Pathology
Tumors
Cancer detection
Fluorescene spectroscopy
Histopathological diagnosis
topic Diagnosis
Fluorescence
Spectroscopy
Tongue carcinoma
Alcohols
Biological organs
Histology
Metabolism
Nondestructive examination
Pathology
Tumors
Cancer detection
Fluorescene spectroscopy
Histopathological diagnosis
description Fluorescence diagnosis of malignant lesions has been showed as an attractive optical technique due especially to its real-time response and a more objective and quantitative evaluation. Even though the oral cavity allows a direct examination many lesions are diagnosed when it is already in advanced stage, compromising the patient prognosis. In this study, the fluorescence spectroscopy was used to the detection of chemically induced carcinoma at the lateral border of the tongue in a hamster model. Two excitations wavelengths in visible region were applied: 442 and 532 nm. All the spectra results were analyzed comparing with the histopathological diagnosis. The better results were achieved with the 442 nm laser excitation. The spectra from carcinoma showed new emission bands and these were used to determined different ratios for a quantitative analysis. Using the 625-645 nm fluorescence range under 442 nm excitation (A3 coefficient) the percentage of false negative was of 9.1%, however the false positive percentage was of 18.5%. The 532 nm excitation provided a better normal tissue detection compared to 442 nm excitation. The ideal clinical condition is probably the use of multiple wavelengths excitation for a broader tissue fluorescence investigation.
publishDate 2005
dc.date.none.fl_str_mv 2005-07-21
2014-05-27T11:21:23Z
2014-05-27T11:21:23Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/conferenceObject
format conferenceObject
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1117/12.588222
Progress in Biomedical Optics and Imaging - Proceedings of SPIE, v. 5689, p. 210-217.
1605-7422
http://hdl.handle.net/11449/68341
10.1117/12.588222
2-s2.0-21844444076
7595315660858413
url http://dx.doi.org/10.1117/12.588222
http://hdl.handle.net/11449/68341
identifier_str_mv Progress in Biomedical Optics and Imaging - Proceedings of SPIE, v. 5689, p. 210-217.
1605-7422
10.1117/12.588222
2-s2.0-21844444076
7595315660858413
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Progress in Biomedical Optics and Imaging - Proceedings of SPIE
0,243
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 210-217
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1808129071516745728