Fluorescence spectroscopy for tongue carcinoma detection: Study in an animal model
Autor(a) principal: | |
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Data de Publicação: | 2005 |
Outros Autores: | , , , |
Tipo de documento: | Artigo de conferência |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1117/12.588222 http://hdl.handle.net/11449/68341 |
Resumo: | Fluorescence diagnosis of malignant lesions has been showed as an attractive optical technique due especially to its real-time response and a more objective and quantitative evaluation. Even though the oral cavity allows a direct examination many lesions are diagnosed when it is already in advanced stage, compromising the patient prognosis. In this study, the fluorescence spectroscopy was used to the detection of chemically induced carcinoma at the lateral border of the tongue in a hamster model. Two excitations wavelengths in visible region were applied: 442 and 532 nm. All the spectra results were analyzed comparing with the histopathological diagnosis. The better results were achieved with the 442 nm laser excitation. The spectra from carcinoma showed new emission bands and these were used to determined different ratios for a quantitative analysis. Using the 625-645 nm fluorescence range under 442 nm excitation (A3 coefficient) the percentage of false negative was of 9.1%, however the false positive percentage was of 18.5%. The 532 nm excitation provided a better normal tissue detection compared to 442 nm excitation. The ideal clinical condition is probably the use of multiple wavelengths excitation for a broader tissue fluorescence investigation. |
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Fluorescence spectroscopy for tongue carcinoma detection: Study in an animal modelDiagnosisFluorescenceSpectroscopyTongue carcinomaAlcoholsBiological organsHistologyMetabolismNondestructive examinationPathologyTumorsCancer detectionFluorescene spectroscopyHistopathological diagnosisFluorescence diagnosis of malignant lesions has been showed as an attractive optical technique due especially to its real-time response and a more objective and quantitative evaluation. Even though the oral cavity allows a direct examination many lesions are diagnosed when it is already in advanced stage, compromising the patient prognosis. In this study, the fluorescence spectroscopy was used to the detection of chemically induced carcinoma at the lateral border of the tongue in a hamster model. Two excitations wavelengths in visible region were applied: 442 and 532 nm. All the spectra results were analyzed comparing with the histopathological diagnosis. The better results were achieved with the 442 nm laser excitation. The spectra from carcinoma showed new emission bands and these were used to determined different ratios for a quantitative analysis. Using the 625-645 nm fluorescence range under 442 nm excitation (A3 coefficient) the percentage of false negative was of 9.1%, however the false positive percentage was of 18.5%. The 532 nm excitation provided a better normal tissue detection compared to 442 nm excitation. The ideal clinical condition is probably the use of multiple wavelengths excitation for a broader tissue fluorescence investigation.Institute de Física de São Carlos Universidade de São PauloFaculdade de Odontologia de São José dos Campos Universidade Estadual PaulistaFaculdade de Odontologia de São José dos Campos Universidade Estadual PaulistaUniversidade de São Paulo (USP)Universidade Estadual Paulista (Unesp)Kurachi, C.Fontana, C. R.Melo, C. A SRosa, L. E B [UNESP]Bagnato, V. S.2014-05-27T11:21:23Z2014-05-27T11:21:23Z2005-07-21info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObject210-217http://dx.doi.org/10.1117/12.588222Progress in Biomedical Optics and Imaging - Proceedings of SPIE, v. 5689, p. 210-217.1605-7422http://hdl.handle.net/11449/6834110.1117/12.5882222-s2.0-218444440767595315660858413Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengProgress in Biomedical Optics and Imaging - Proceedings of SPIE0,243info:eu-repo/semantics/openAccess2021-10-23T21:44:20Zoai:repositorio.unesp.br:11449/68341Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T19:27:11.571125Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Fluorescence spectroscopy for tongue carcinoma detection: Study in an animal model |
title |
Fluorescence spectroscopy for tongue carcinoma detection: Study in an animal model |
spellingShingle |
Fluorescence spectroscopy for tongue carcinoma detection: Study in an animal model Kurachi, C. Diagnosis Fluorescence Spectroscopy Tongue carcinoma Alcohols Biological organs Histology Metabolism Nondestructive examination Pathology Tumors Cancer detection Fluorescene spectroscopy Histopathological diagnosis |
title_short |
Fluorescence spectroscopy for tongue carcinoma detection: Study in an animal model |
title_full |
Fluorescence spectroscopy for tongue carcinoma detection: Study in an animal model |
title_fullStr |
Fluorescence spectroscopy for tongue carcinoma detection: Study in an animal model |
title_full_unstemmed |
Fluorescence spectroscopy for tongue carcinoma detection: Study in an animal model |
title_sort |
Fluorescence spectroscopy for tongue carcinoma detection: Study in an animal model |
author |
Kurachi, C. |
author_facet |
Kurachi, C. Fontana, C. R. Melo, C. A S Rosa, L. E B [UNESP] Bagnato, V. S. |
author_role |
author |
author2 |
Fontana, C. R. Melo, C. A S Rosa, L. E B [UNESP] Bagnato, V. S. |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Kurachi, C. Fontana, C. R. Melo, C. A S Rosa, L. E B [UNESP] Bagnato, V. S. |
dc.subject.por.fl_str_mv |
Diagnosis Fluorescence Spectroscopy Tongue carcinoma Alcohols Biological organs Histology Metabolism Nondestructive examination Pathology Tumors Cancer detection Fluorescene spectroscopy Histopathological diagnosis |
topic |
Diagnosis Fluorescence Spectroscopy Tongue carcinoma Alcohols Biological organs Histology Metabolism Nondestructive examination Pathology Tumors Cancer detection Fluorescene spectroscopy Histopathological diagnosis |
description |
Fluorescence diagnosis of malignant lesions has been showed as an attractive optical technique due especially to its real-time response and a more objective and quantitative evaluation. Even though the oral cavity allows a direct examination many lesions are diagnosed when it is already in advanced stage, compromising the patient prognosis. In this study, the fluorescence spectroscopy was used to the detection of chemically induced carcinoma at the lateral border of the tongue in a hamster model. Two excitations wavelengths in visible region were applied: 442 and 532 nm. All the spectra results were analyzed comparing with the histopathological diagnosis. The better results were achieved with the 442 nm laser excitation. The spectra from carcinoma showed new emission bands and these were used to determined different ratios for a quantitative analysis. Using the 625-645 nm fluorescence range under 442 nm excitation (A3 coefficient) the percentage of false negative was of 9.1%, however the false positive percentage was of 18.5%. The 532 nm excitation provided a better normal tissue detection compared to 442 nm excitation. The ideal clinical condition is probably the use of multiple wavelengths excitation for a broader tissue fluorescence investigation. |
publishDate |
2005 |
dc.date.none.fl_str_mv |
2005-07-21 2014-05-27T11:21:23Z 2014-05-27T11:21:23Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/conferenceObject |
format |
conferenceObject |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1117/12.588222 Progress in Biomedical Optics and Imaging - Proceedings of SPIE, v. 5689, p. 210-217. 1605-7422 http://hdl.handle.net/11449/68341 10.1117/12.588222 2-s2.0-21844444076 7595315660858413 |
url |
http://dx.doi.org/10.1117/12.588222 http://hdl.handle.net/11449/68341 |
identifier_str_mv |
Progress in Biomedical Optics and Imaging - Proceedings of SPIE, v. 5689, p. 210-217. 1605-7422 10.1117/12.588222 2-s2.0-21844444076 7595315660858413 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Progress in Biomedical Optics and Imaging - Proceedings of SPIE 0,243 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
210-217 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808129071516745728 |