Effects of octenidine applied alone or mixed with sodium hypochlorite on eukaryotic cells

Detalhes bibliográficos
Autor(a) principal: Coaguila-Llerena, H. [UNESP]
Data de Publicação: 2020
Outros Autores: Rodrigues, E. M. [UNESP], Santos, C. S. [UNESP], Ramos, S. G., Medeiros, M. C. [UNESP], Chavez-Andrade, G. M. [UNESP], Guerreiro-Tanomaru, J. M. [UNESP], Tanomaru-Filho, M. [UNESP], Faria, G. [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1111/iej.13347
http://hdl.handle.net/11449/200726
Resumo: Aim: To assess the effects of octenidine dihydrochloride (OCT) on eukaryotic cells and the cytotoxicity of OCT associated with sodium hypochlorite – NaOCl (NaOCl/OCT). Methodology: L929 fibroblasts and human osteoblast-like cells (Saos-2) were exposed to 0.1% OCT, 2% CHX, 2.5% NaOCl, 5.25% NaOCl and mixtures of 5.25% NaOCl and 0.1% OCT (NaOCl/OCT) at 90 : 10, 80 : 20 and 50 : 50 ratios. Cell viability was assessed by methyl-thiazol-tetrazolium (MTT) and neutral red (NR) assays; type of cell death, by flow cytometry; cytoskeleton, by actin and α-tubulin fluorescence; and alkaline phosphatase (ALP) activity, by thymolphthalein release. The data were analysed by two-way ANOVA and Bonferroni tests (α = 0.05). Results: MTT and NR assays revealed that 0.1% OCT had the lowest cytotoxicity (P < 0.05), followed by 2% CHX (P < 0.05). The 2.5% NaOCl, NaOCl/OCT 80 : 20 and NaOCl/OCT 50 : 50 solutions had intermediate cytotoxicity. NaOCl 5.25% and NaOCl/OCT 90 : 10 had the highest cytotoxicity (P < 0.05). The OCT group had a higher percentage of viable cells than the NaOCl and CHX groups (P < 0.05), and induced apoptosis at higher doses. The cytoskeleton alterations were observed at 0.12%, 0.6% and 2.02% for the NaOCl, CHX and OCT groups, respectively. The solutions did not induce ALP activity. Conclusion: Octenidine dihydrochloride was less cytotoxic, induced apoptosis at higher doses, caused few changes in the cytoskeleton and did not induce alkaline phosphatase activity. In addition, octenidine dihydrochloride reduced the cytotoxicity of 5.25% NaOCl when combined at 20 and 50%.
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spelling Effects of octenidine applied alone or mixed with sodium hypochlorite on eukaryotic cellscell culture techniquesmaterials testingoctenidineroot canal therapysodium hypochloriteAim: To assess the effects of octenidine dihydrochloride (OCT) on eukaryotic cells and the cytotoxicity of OCT associated with sodium hypochlorite – NaOCl (NaOCl/OCT). Methodology: L929 fibroblasts and human osteoblast-like cells (Saos-2) were exposed to 0.1% OCT, 2% CHX, 2.5% NaOCl, 5.25% NaOCl and mixtures of 5.25% NaOCl and 0.1% OCT (NaOCl/OCT) at 90 : 10, 80 : 20 and 50 : 50 ratios. Cell viability was assessed by methyl-thiazol-tetrazolium (MTT) and neutral red (NR) assays; type of cell death, by flow cytometry; cytoskeleton, by actin and α-tubulin fluorescence; and alkaline phosphatase (ALP) activity, by thymolphthalein release. The data were analysed by two-way ANOVA and Bonferroni tests (α = 0.05). Results: MTT and NR assays revealed that 0.1% OCT had the lowest cytotoxicity (P < 0.05), followed by 2% CHX (P < 0.05). The 2.5% NaOCl, NaOCl/OCT 80 : 20 and NaOCl/OCT 50 : 50 solutions had intermediate cytotoxicity. NaOCl 5.25% and NaOCl/OCT 90 : 10 had the highest cytotoxicity (P < 0.05). The OCT group had a higher percentage of viable cells than the NaOCl and CHX groups (P < 0.05), and induced apoptosis at higher doses. The cytoskeleton alterations were observed at 0.12%, 0.6% and 2.02% for the NaOCl, CHX and OCT groups, respectively. The solutions did not induce ALP activity. Conclusion: Octenidine dihydrochloride was less cytotoxic, induced apoptosis at higher doses, caused few changes in the cytoskeleton and did not induce alkaline phosphatase activity. In addition, octenidine dihydrochloride reduced the cytotoxicity of 5.25% NaOCl when combined at 20 and 50%.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Department of Restorative Dentistry Araraquara School of Dentistry São Paulo State University – UNESPDepartment of Pathology Ribeirão Preto Medical School University of São Paulo – USPDepartment of Oral Diagnosis and Surgery Araraquara School of Dentistry São Paulo State University – UNESPDepartment of Restorative Dentistry Araraquara School of Dentistry São Paulo State University – UNESPDepartment of Oral Diagnosis and Surgery Araraquara School of Dentistry São Paulo State University – UNESPFAPESP: 2018/24662-6CNPq: 48032Universidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)Coaguila-Llerena, H. [UNESP]Rodrigues, E. M. [UNESP]Santos, C. S. [UNESP]Ramos, S. G.Medeiros, M. C. [UNESP]Chavez-Andrade, G. M. [UNESP]Guerreiro-Tanomaru, J. M. [UNESP]Tanomaru-Filho, M. [UNESP]Faria, G. [UNESP]2020-12-12T02:14:25Z2020-12-12T02:14:25Z2020-09-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1264-1274http://dx.doi.org/10.1111/iej.13347International Endodontic Journal, v. 53, n. 9, p. 1264-1274, 2020.1365-25910143-2885http://hdl.handle.net/11449/20072610.1111/iej.133472-s2.0-85087714207Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengInternational Endodontic Journalinfo:eu-repo/semantics/openAccess2024-09-27T18:03:53Zoai:repositorio.unesp.br:11449/200726Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-27T18:03:53Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Effects of octenidine applied alone or mixed with sodium hypochlorite on eukaryotic cells
title Effects of octenidine applied alone or mixed with sodium hypochlorite on eukaryotic cells
spellingShingle Effects of octenidine applied alone or mixed with sodium hypochlorite on eukaryotic cells
Coaguila-Llerena, H. [UNESP]
cell culture techniques
materials testing
octenidine
root canal therapy
sodium hypochlorite
title_short Effects of octenidine applied alone or mixed with sodium hypochlorite on eukaryotic cells
title_full Effects of octenidine applied alone or mixed with sodium hypochlorite on eukaryotic cells
title_fullStr Effects of octenidine applied alone or mixed with sodium hypochlorite on eukaryotic cells
title_full_unstemmed Effects of octenidine applied alone or mixed with sodium hypochlorite on eukaryotic cells
title_sort Effects of octenidine applied alone or mixed with sodium hypochlorite on eukaryotic cells
author Coaguila-Llerena, H. [UNESP]
author_facet Coaguila-Llerena, H. [UNESP]
Rodrigues, E. M. [UNESP]
Santos, C. S. [UNESP]
Ramos, S. G.
Medeiros, M. C. [UNESP]
Chavez-Andrade, G. M. [UNESP]
Guerreiro-Tanomaru, J. M. [UNESP]
Tanomaru-Filho, M. [UNESP]
Faria, G. [UNESP]
author_role author
author2 Rodrigues, E. M. [UNESP]
Santos, C. S. [UNESP]
Ramos, S. G.
Medeiros, M. C. [UNESP]
Chavez-Andrade, G. M. [UNESP]
Guerreiro-Tanomaru, J. M. [UNESP]
Tanomaru-Filho, M. [UNESP]
Faria, G. [UNESP]
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Universidade de São Paulo (USP)
dc.contributor.author.fl_str_mv Coaguila-Llerena, H. [UNESP]
Rodrigues, E. M. [UNESP]
Santos, C. S. [UNESP]
Ramos, S. G.
Medeiros, M. C. [UNESP]
Chavez-Andrade, G. M. [UNESP]
Guerreiro-Tanomaru, J. M. [UNESP]
Tanomaru-Filho, M. [UNESP]
Faria, G. [UNESP]
dc.subject.por.fl_str_mv cell culture techniques
materials testing
octenidine
root canal therapy
sodium hypochlorite
topic cell culture techniques
materials testing
octenidine
root canal therapy
sodium hypochlorite
description Aim: To assess the effects of octenidine dihydrochloride (OCT) on eukaryotic cells and the cytotoxicity of OCT associated with sodium hypochlorite – NaOCl (NaOCl/OCT). Methodology: L929 fibroblasts and human osteoblast-like cells (Saos-2) were exposed to 0.1% OCT, 2% CHX, 2.5% NaOCl, 5.25% NaOCl and mixtures of 5.25% NaOCl and 0.1% OCT (NaOCl/OCT) at 90 : 10, 80 : 20 and 50 : 50 ratios. Cell viability was assessed by methyl-thiazol-tetrazolium (MTT) and neutral red (NR) assays; type of cell death, by flow cytometry; cytoskeleton, by actin and α-tubulin fluorescence; and alkaline phosphatase (ALP) activity, by thymolphthalein release. The data were analysed by two-way ANOVA and Bonferroni tests (α = 0.05). Results: MTT and NR assays revealed that 0.1% OCT had the lowest cytotoxicity (P < 0.05), followed by 2% CHX (P < 0.05). The 2.5% NaOCl, NaOCl/OCT 80 : 20 and NaOCl/OCT 50 : 50 solutions had intermediate cytotoxicity. NaOCl 5.25% and NaOCl/OCT 90 : 10 had the highest cytotoxicity (P < 0.05). The OCT group had a higher percentage of viable cells than the NaOCl and CHX groups (P < 0.05), and induced apoptosis at higher doses. The cytoskeleton alterations were observed at 0.12%, 0.6% and 2.02% for the NaOCl, CHX and OCT groups, respectively. The solutions did not induce ALP activity. Conclusion: Octenidine dihydrochloride was less cytotoxic, induced apoptosis at higher doses, caused few changes in the cytoskeleton and did not induce alkaline phosphatase activity. In addition, octenidine dihydrochloride reduced the cytotoxicity of 5.25% NaOCl when combined at 20 and 50%.
publishDate 2020
dc.date.none.fl_str_mv 2020-12-12T02:14:25Z
2020-12-12T02:14:25Z
2020-09-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1111/iej.13347
International Endodontic Journal, v. 53, n. 9, p. 1264-1274, 2020.
1365-2591
0143-2885
http://hdl.handle.net/11449/200726
10.1111/iej.13347
2-s2.0-85087714207
url http://dx.doi.org/10.1111/iej.13347
http://hdl.handle.net/11449/200726
identifier_str_mv International Endodontic Journal, v. 53, n. 9, p. 1264-1274, 2020.
1365-2591
0143-2885
10.1111/iej.13347
2-s2.0-85087714207
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv International Endodontic Journal
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 1264-1274
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv repositoriounesp@unesp.br
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