Effects of octenidine applied alone or mixed with sodium hypochlorite on eukaryotic cells
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1111/iej.13347 http://hdl.handle.net/11449/200726 |
Resumo: | Aim: To assess the effects of octenidine dihydrochloride (OCT) on eukaryotic cells and the cytotoxicity of OCT associated with sodium hypochlorite – NaOCl (NaOCl/OCT). Methodology: L929 fibroblasts and human osteoblast-like cells (Saos-2) were exposed to 0.1% OCT, 2% CHX, 2.5% NaOCl, 5.25% NaOCl and mixtures of 5.25% NaOCl and 0.1% OCT (NaOCl/OCT) at 90 : 10, 80 : 20 and 50 : 50 ratios. Cell viability was assessed by methyl-thiazol-tetrazolium (MTT) and neutral red (NR) assays; type of cell death, by flow cytometry; cytoskeleton, by actin and α-tubulin fluorescence; and alkaline phosphatase (ALP) activity, by thymolphthalein release. The data were analysed by two-way ANOVA and Bonferroni tests (α = 0.05). Results: MTT and NR assays revealed that 0.1% OCT had the lowest cytotoxicity (P < 0.05), followed by 2% CHX (P < 0.05). The 2.5% NaOCl, NaOCl/OCT 80 : 20 and NaOCl/OCT 50 : 50 solutions had intermediate cytotoxicity. NaOCl 5.25% and NaOCl/OCT 90 : 10 had the highest cytotoxicity (P < 0.05). The OCT group had a higher percentage of viable cells than the NaOCl and CHX groups (P < 0.05), and induced apoptosis at higher doses. The cytoskeleton alterations were observed at 0.12%, 0.6% and 2.02% for the NaOCl, CHX and OCT groups, respectively. The solutions did not induce ALP activity. Conclusion: Octenidine dihydrochloride was less cytotoxic, induced apoptosis at higher doses, caused few changes in the cytoskeleton and did not induce alkaline phosphatase activity. In addition, octenidine dihydrochloride reduced the cytotoxicity of 5.25% NaOCl when combined at 20 and 50%. |
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Effects of octenidine applied alone or mixed with sodium hypochlorite on eukaryotic cellscell culture techniquesmaterials testingoctenidineroot canal therapysodium hypochloriteAim: To assess the effects of octenidine dihydrochloride (OCT) on eukaryotic cells and the cytotoxicity of OCT associated with sodium hypochlorite – NaOCl (NaOCl/OCT). Methodology: L929 fibroblasts and human osteoblast-like cells (Saos-2) were exposed to 0.1% OCT, 2% CHX, 2.5% NaOCl, 5.25% NaOCl and mixtures of 5.25% NaOCl and 0.1% OCT (NaOCl/OCT) at 90 : 10, 80 : 20 and 50 : 50 ratios. Cell viability was assessed by methyl-thiazol-tetrazolium (MTT) and neutral red (NR) assays; type of cell death, by flow cytometry; cytoskeleton, by actin and α-tubulin fluorescence; and alkaline phosphatase (ALP) activity, by thymolphthalein release. The data were analysed by two-way ANOVA and Bonferroni tests (α = 0.05). Results: MTT and NR assays revealed that 0.1% OCT had the lowest cytotoxicity (P < 0.05), followed by 2% CHX (P < 0.05). The 2.5% NaOCl, NaOCl/OCT 80 : 20 and NaOCl/OCT 50 : 50 solutions had intermediate cytotoxicity. NaOCl 5.25% and NaOCl/OCT 90 : 10 had the highest cytotoxicity (P < 0.05). The OCT group had a higher percentage of viable cells than the NaOCl and CHX groups (P < 0.05), and induced apoptosis at higher doses. The cytoskeleton alterations were observed at 0.12%, 0.6% and 2.02% for the NaOCl, CHX and OCT groups, respectively. The solutions did not induce ALP activity. Conclusion: Octenidine dihydrochloride was less cytotoxic, induced apoptosis at higher doses, caused few changes in the cytoskeleton and did not induce alkaline phosphatase activity. In addition, octenidine dihydrochloride reduced the cytotoxicity of 5.25% NaOCl when combined at 20 and 50%.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Department of Restorative Dentistry Araraquara School of Dentistry São Paulo State University – UNESPDepartment of Pathology Ribeirão Preto Medical School University of São Paulo – USPDepartment of Oral Diagnosis and Surgery Araraquara School of Dentistry São Paulo State University – UNESPDepartment of Restorative Dentistry Araraquara School of Dentistry São Paulo State University – UNESPDepartment of Oral Diagnosis and Surgery Araraquara School of Dentistry São Paulo State University – UNESPFAPESP: 2018/24662-6CNPq: 48032Universidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)Coaguila-Llerena, H. [UNESP]Rodrigues, E. M. [UNESP]Santos, C. S. [UNESP]Ramos, S. G.Medeiros, M. C. [UNESP]Chavez-Andrade, G. M. [UNESP]Guerreiro-Tanomaru, J. M. [UNESP]Tanomaru-Filho, M. [UNESP]Faria, G. [UNESP]2020-12-12T02:14:25Z2020-12-12T02:14:25Z2020-09-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1264-1274http://dx.doi.org/10.1111/iej.13347International Endodontic Journal, v. 53, n. 9, p. 1264-1274, 2020.1365-25910143-2885http://hdl.handle.net/11449/20072610.1111/iej.133472-s2.0-85087714207Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengInternational Endodontic Journalinfo:eu-repo/semantics/openAccess2024-09-27T18:03:53Zoai:repositorio.unesp.br:11449/200726Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-27T18:03:53Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Effects of octenidine applied alone or mixed with sodium hypochlorite on eukaryotic cells |
title |
Effects of octenidine applied alone or mixed with sodium hypochlorite on eukaryotic cells |
spellingShingle |
Effects of octenidine applied alone or mixed with sodium hypochlorite on eukaryotic cells Coaguila-Llerena, H. [UNESP] cell culture techniques materials testing octenidine root canal therapy sodium hypochlorite |
title_short |
Effects of octenidine applied alone or mixed with sodium hypochlorite on eukaryotic cells |
title_full |
Effects of octenidine applied alone or mixed with sodium hypochlorite on eukaryotic cells |
title_fullStr |
Effects of octenidine applied alone or mixed with sodium hypochlorite on eukaryotic cells |
title_full_unstemmed |
Effects of octenidine applied alone or mixed with sodium hypochlorite on eukaryotic cells |
title_sort |
Effects of octenidine applied alone or mixed with sodium hypochlorite on eukaryotic cells |
author |
Coaguila-Llerena, H. [UNESP] |
author_facet |
Coaguila-Llerena, H. [UNESP] Rodrigues, E. M. [UNESP] Santos, C. S. [UNESP] Ramos, S. G. Medeiros, M. C. [UNESP] Chavez-Andrade, G. M. [UNESP] Guerreiro-Tanomaru, J. M. [UNESP] Tanomaru-Filho, M. [UNESP] Faria, G. [UNESP] |
author_role |
author |
author2 |
Rodrigues, E. M. [UNESP] Santos, C. S. [UNESP] Ramos, S. G. Medeiros, M. C. [UNESP] Chavez-Andrade, G. M. [UNESP] Guerreiro-Tanomaru, J. M. [UNESP] Tanomaru-Filho, M. [UNESP] Faria, G. [UNESP] |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Coaguila-Llerena, H. [UNESP] Rodrigues, E. M. [UNESP] Santos, C. S. [UNESP] Ramos, S. G. Medeiros, M. C. [UNESP] Chavez-Andrade, G. M. [UNESP] Guerreiro-Tanomaru, J. M. [UNESP] Tanomaru-Filho, M. [UNESP] Faria, G. [UNESP] |
dc.subject.por.fl_str_mv |
cell culture techniques materials testing octenidine root canal therapy sodium hypochlorite |
topic |
cell culture techniques materials testing octenidine root canal therapy sodium hypochlorite |
description |
Aim: To assess the effects of octenidine dihydrochloride (OCT) on eukaryotic cells and the cytotoxicity of OCT associated with sodium hypochlorite – NaOCl (NaOCl/OCT). Methodology: L929 fibroblasts and human osteoblast-like cells (Saos-2) were exposed to 0.1% OCT, 2% CHX, 2.5% NaOCl, 5.25% NaOCl and mixtures of 5.25% NaOCl and 0.1% OCT (NaOCl/OCT) at 90 : 10, 80 : 20 and 50 : 50 ratios. Cell viability was assessed by methyl-thiazol-tetrazolium (MTT) and neutral red (NR) assays; type of cell death, by flow cytometry; cytoskeleton, by actin and α-tubulin fluorescence; and alkaline phosphatase (ALP) activity, by thymolphthalein release. The data were analysed by two-way ANOVA and Bonferroni tests (α = 0.05). Results: MTT and NR assays revealed that 0.1% OCT had the lowest cytotoxicity (P < 0.05), followed by 2% CHX (P < 0.05). The 2.5% NaOCl, NaOCl/OCT 80 : 20 and NaOCl/OCT 50 : 50 solutions had intermediate cytotoxicity. NaOCl 5.25% and NaOCl/OCT 90 : 10 had the highest cytotoxicity (P < 0.05). The OCT group had a higher percentage of viable cells than the NaOCl and CHX groups (P < 0.05), and induced apoptosis at higher doses. The cytoskeleton alterations were observed at 0.12%, 0.6% and 2.02% for the NaOCl, CHX and OCT groups, respectively. The solutions did not induce ALP activity. Conclusion: Octenidine dihydrochloride was less cytotoxic, induced apoptosis at higher doses, caused few changes in the cytoskeleton and did not induce alkaline phosphatase activity. In addition, octenidine dihydrochloride reduced the cytotoxicity of 5.25% NaOCl when combined at 20 and 50%. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-12T02:14:25Z 2020-12-12T02:14:25Z 2020-09-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1111/iej.13347 International Endodontic Journal, v. 53, n. 9, p. 1264-1274, 2020. 1365-2591 0143-2885 http://hdl.handle.net/11449/200726 10.1111/iej.13347 2-s2.0-85087714207 |
url |
http://dx.doi.org/10.1111/iej.13347 http://hdl.handle.net/11449/200726 |
identifier_str_mv |
International Endodontic Journal, v. 53, n. 9, p. 1264-1274, 2020. 1365-2591 0143-2885 10.1111/iej.13347 2-s2.0-85087714207 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
International Endodontic Journal |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1264-1274 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
repositoriounesp@unesp.br |
_version_ |
1813546439098236928 |