A presença da proteína nuclear específica neuronal (NeuN) no sistema de temporização circadiano do macaco capuchinho (Cebus apella)
Autor(a) principal: | |
---|---|
Data de Publicação: | 2010 |
Outros Autores: | , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng por |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://hdl.handle.net/11449/226203 |
Resumo: | Background and objective: The circadian timing system (CTS) is composed of a group of specialized neuronal structures that establish a temporal organization of physiological and behavioral processes within precise patterns. The central components of this system are the suprachiasmatic nucleus of the hypothalamus (SCN) and the intergeniculate leaflet of the thalamus (IGL). The objective of this study was to verify the presence of the neuron-specific nuclear protein (NeuN) in the circadian timing system of capuchin monkeys (Cebus apella) using immunohistochemical techniques. Methods: Capuchin monkeys (Cebus apella) were anesthetized and transcardially perfused with 4% paraformaldehyde in 0.1 M phosphate buffer, and then their brains were removed and frozen. A microtome was used to make 30 μm sections in the coronal plane. One of the series was used for Nissl staining (thionin) to demarcate the cytoarchitecture, and the remainders of the sections were processed for immunohistochemical detection of NeuN (ABC protocol). Results: NeuN-positive neurons were observed in the suprachiasmatic nucleus of the capuchin monkey. The pregeniculate nucleus (PGN), a structure equivalent to the ventral lateral geniculate nucleus (vLGN) and to the IGL in rodents, did not have any NeuN-positive neurons. Conclusions: In this primate species, only the suprachiasmatic nucleus neurons of the central structures of the circadian timing system express the NeuN protein. |
id |
UNSP_e6be9b9885e7c1ae1edae03736111e85 |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/226203 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
A presença da proteína nuclear específica neuronal (NeuN) no sistema de temporização circadiano do macaco capuchinho (Cebus apella)The presence of neuronal-specific nuclear protein (NeuN) in the circadian timing system of the capuchin monkey (Cebus apella)AnimalsCebusCircadian rhythmGeniculate bodiesImmunohistochemistry/methodsNeuronsNuclear proteinsSuprachiasmatic nucleusBackground and objective: The circadian timing system (CTS) is composed of a group of specialized neuronal structures that establish a temporal organization of physiological and behavioral processes within precise patterns. The central components of this system are the suprachiasmatic nucleus of the hypothalamus (SCN) and the intergeniculate leaflet of the thalamus (IGL). The objective of this study was to verify the presence of the neuron-specific nuclear protein (NeuN) in the circadian timing system of capuchin monkeys (Cebus apella) using immunohistochemical techniques. Methods: Capuchin monkeys (Cebus apella) were anesthetized and transcardially perfused with 4% paraformaldehyde in 0.1 M phosphate buffer, and then their brains were removed and frozen. A microtome was used to make 30 μm sections in the coronal plane. One of the series was used for Nissl staining (thionin) to demarcate the cytoarchitecture, and the remainders of the sections were processed for immunohistochemical detection of NeuN (ABC protocol). Results: NeuN-positive neurons were observed in the suprachiasmatic nucleus of the capuchin monkey. The pregeniculate nucleus (PGN), a structure equivalent to the ventral lateral geniculate nucleus (vLGN) and to the IGL in rodents, did not have any NeuN-positive neurons. Conclusions: In this primate species, only the suprachiasmatic nucleus neurons of the central structures of the circadian timing system express the NeuN protein.Laboratório de Cronobiologia Centro de Biociências Universidade Federal do Rio Grande do Norte - UFRN, Natal (RN)Departamento de Anatomia Universidade de São Paulo - USP, São Paulo (SP)Departamento de Ciências Básicas Universidade Estadual Paulista 'Júlio de Mesquita Filho' - UNESP, Araçatuba (SP)Departamento de Ciências Básicas Universidade Estadual Paulista 'Júlio de Mesquita Filho' - UNESP, Araçatuba (SP)Universidade Federal do Rio Grande do Norte - UFRNUniversidade de São Paulo (USP)Universidade Estadual Paulista (UNESP)do Nascimento, Rayane Bartira SilvaBorda, Janaína SiqueiraEngelberth, Rovena Clara Galvão Januáriode Medeiros, Raysa OliveiraFrazão, RenataPinato, Lucianade Pontes, André Luiz Bezerrado Nascimento Jr., Expedito SilvaNogueira, Maria InêsCruz-Rizzolo, Roelf Justino [UNESP]Costa, Miriam Stela Maris de OliveiraCavalcante, Jeferson de Souza2022-04-28T22:02:07Z2022-04-28T22:02:07Z2010-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article36-39Sleep Science, v. 3, n. 1, p. 36-39, 2010.1984-06591984-0063http://hdl.handle.net/11449/2262032-s2.0-78651458232Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengporSleep Scienceinfo:eu-repo/semantics/openAccess2022-04-28T22:02:07Zoai:repositorio.unesp.br:11449/226203Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T17:29:55.426421Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
A presença da proteína nuclear específica neuronal (NeuN) no sistema de temporização circadiano do macaco capuchinho (Cebus apella) The presence of neuronal-specific nuclear protein (NeuN) in the circadian timing system of the capuchin monkey (Cebus apella) |
title |
A presença da proteína nuclear específica neuronal (NeuN) no sistema de temporização circadiano do macaco capuchinho (Cebus apella) |
spellingShingle |
A presença da proteína nuclear específica neuronal (NeuN) no sistema de temporização circadiano do macaco capuchinho (Cebus apella) do Nascimento, Rayane Bartira Silva Animals Cebus Circadian rhythm Geniculate bodies Immunohistochemistry/methods Neurons Nuclear proteins Suprachiasmatic nucleus |
title_short |
A presença da proteína nuclear específica neuronal (NeuN) no sistema de temporização circadiano do macaco capuchinho (Cebus apella) |
title_full |
A presença da proteína nuclear específica neuronal (NeuN) no sistema de temporização circadiano do macaco capuchinho (Cebus apella) |
title_fullStr |
A presença da proteína nuclear específica neuronal (NeuN) no sistema de temporização circadiano do macaco capuchinho (Cebus apella) |
title_full_unstemmed |
A presença da proteína nuclear específica neuronal (NeuN) no sistema de temporização circadiano do macaco capuchinho (Cebus apella) |
title_sort |
A presença da proteína nuclear específica neuronal (NeuN) no sistema de temporização circadiano do macaco capuchinho (Cebus apella) |
author |
do Nascimento, Rayane Bartira Silva |
author_facet |
do Nascimento, Rayane Bartira Silva Borda, Janaína Siqueira Engelberth, Rovena Clara Galvão Januário de Medeiros, Raysa Oliveira Frazão, Renata Pinato, Luciana de Pontes, André Luiz Bezerra do Nascimento Jr., Expedito Silva Nogueira, Maria Inês Cruz-Rizzolo, Roelf Justino [UNESP] Costa, Miriam Stela Maris de Oliveira Cavalcante, Jeferson de Souza |
author_role |
author |
author2 |
Borda, Janaína Siqueira Engelberth, Rovena Clara Galvão Januário de Medeiros, Raysa Oliveira Frazão, Renata Pinato, Luciana de Pontes, André Luiz Bezerra do Nascimento Jr., Expedito Silva Nogueira, Maria Inês Cruz-Rizzolo, Roelf Justino [UNESP] Costa, Miriam Stela Maris de Oliveira Cavalcante, Jeferson de Souza |
author2_role |
author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal do Rio Grande do Norte - UFRN Universidade de São Paulo (USP) Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
do Nascimento, Rayane Bartira Silva Borda, Janaína Siqueira Engelberth, Rovena Clara Galvão Januário de Medeiros, Raysa Oliveira Frazão, Renata Pinato, Luciana de Pontes, André Luiz Bezerra do Nascimento Jr., Expedito Silva Nogueira, Maria Inês Cruz-Rizzolo, Roelf Justino [UNESP] Costa, Miriam Stela Maris de Oliveira Cavalcante, Jeferson de Souza |
dc.subject.por.fl_str_mv |
Animals Cebus Circadian rhythm Geniculate bodies Immunohistochemistry/methods Neurons Nuclear proteins Suprachiasmatic nucleus |
topic |
Animals Cebus Circadian rhythm Geniculate bodies Immunohistochemistry/methods Neurons Nuclear proteins Suprachiasmatic nucleus |
description |
Background and objective: The circadian timing system (CTS) is composed of a group of specialized neuronal structures that establish a temporal organization of physiological and behavioral processes within precise patterns. The central components of this system are the suprachiasmatic nucleus of the hypothalamus (SCN) and the intergeniculate leaflet of the thalamus (IGL). The objective of this study was to verify the presence of the neuron-specific nuclear protein (NeuN) in the circadian timing system of capuchin monkeys (Cebus apella) using immunohistochemical techniques. Methods: Capuchin monkeys (Cebus apella) were anesthetized and transcardially perfused with 4% paraformaldehyde in 0.1 M phosphate buffer, and then their brains were removed and frozen. A microtome was used to make 30 μm sections in the coronal plane. One of the series was used for Nissl staining (thionin) to demarcate the cytoarchitecture, and the remainders of the sections were processed for immunohistochemical detection of NeuN (ABC protocol). Results: NeuN-positive neurons were observed in the suprachiasmatic nucleus of the capuchin monkey. The pregeniculate nucleus (PGN), a structure equivalent to the ventral lateral geniculate nucleus (vLGN) and to the IGL in rodents, did not have any NeuN-positive neurons. Conclusions: In this primate species, only the suprachiasmatic nucleus neurons of the central structures of the circadian timing system express the NeuN protein. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010-12-01 2022-04-28T22:02:07Z 2022-04-28T22:02:07Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Sleep Science, v. 3, n. 1, p. 36-39, 2010. 1984-0659 1984-0063 http://hdl.handle.net/11449/226203 2-s2.0-78651458232 |
identifier_str_mv |
Sleep Science, v. 3, n. 1, p. 36-39, 2010. 1984-0659 1984-0063 2-s2.0-78651458232 |
url |
http://hdl.handle.net/11449/226203 |
dc.language.iso.fl_str_mv |
eng por |
language |
eng por |
dc.relation.none.fl_str_mv |
Sleep Science |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
36-39 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128818643206144 |