Biochemical and hormonal profile of the amniotic fluid from Nelore calves conceived by different reproduction biotechnologies

Detalhes bibliográficos
Autor(a) principal: Piagentini, Marcelo [UNESP]
Data de Publicação: 2012
Outros Autores: Prestes, Nereu Carlos [UNESP], Moya-Araujo, Carla Fredrichsen, Araujo, Gustavo Henrique Marques, Takahira, Regina Kiomi [UNESP], Oba, Eunice [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.7213/academica.7699
http://hdl.handle.net/11449/140576
Resumo: This study aimed to quantify biochemical constituents and hormonal concentrations in the amniotic fluid of Nelore calves conceived by different reproduction biotechnologies. Sixty animals were used and divided into: 1 – Twenty Nelore cows pregnant of calves obtained by artificial insemination; 2 – Twenty cows pregnant with Nelore calves obtained by embryo transference; 3 – Twenty cows pregnant with Nelore calves obtained by in vitro production. During the expulsion phase, the amnion was punctured and 15 mL of fluid were collected. The biochemical evaluation was performed with commercial kits in accordance to the manufacturer’s recommendations. It was used the radioimmunoassay to assess the progesterone and testosterone levels. The statistical analysis used was the variance analysis and Tukey (5% significance level). Mean urea and creatinine when comparing the three groups showed that group 3 had higher values of these two metabolites, suggesting that the kidney maturity is suitable. For gamma-glutamyltransferase, group 3 had a lower value compared to group 1. This enzyme is related to metabolism, may be inferred that the metabolic activity is high in animal production in vitro. The glucose values did not differ between the groups, suggests that the maternal-fetal exchanges were suitable for the power supply to the fetus. Lower chloride concentration was showed in group 3. Sodium and potassium were found in greater quantities in group 3 when compared to other groups. There was no significant difference for the testosterone and progesterone. The results obtained in biochemical analysis can conclude that the renal and liver activity and maternal-fetal exchange are normal in the different groups.
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spelling Biochemical and hormonal profile of the amniotic fluid from Nelore calves conceived by different reproduction biotechnologiesPerfil bioquímico e hormonal do líquido amniótico de bezerros Nelore concebidos por diferentes biotecnologiasAmniotic fluidLaboratorial analysesNeloreLíquido amnióticoAnálise laboratorialNeloreThis study aimed to quantify biochemical constituents and hormonal concentrations in the amniotic fluid of Nelore calves conceived by different reproduction biotechnologies. Sixty animals were used and divided into: 1 – Twenty Nelore cows pregnant of calves obtained by artificial insemination; 2 – Twenty cows pregnant with Nelore calves obtained by embryo transference; 3 – Twenty cows pregnant with Nelore calves obtained by in vitro production. During the expulsion phase, the amnion was punctured and 15 mL of fluid were collected. The biochemical evaluation was performed with commercial kits in accordance to the manufacturer’s recommendations. It was used the radioimmunoassay to assess the progesterone and testosterone levels. The statistical analysis used was the variance analysis and Tukey (5% significance level). Mean urea and creatinine when comparing the three groups showed that group 3 had higher values of these two metabolites, suggesting that the kidney maturity is suitable. For gamma-glutamyltransferase, group 3 had a lower value compared to group 1. This enzyme is related to metabolism, may be inferred that the metabolic activity is high in animal production in vitro. The glucose values did not differ between the groups, suggests that the maternal-fetal exchanges were suitable for the power supply to the fetus. Lower chloride concentration was showed in group 3. Sodium and potassium were found in greater quantities in group 3 when compared to other groups. There was no significant difference for the testosterone and progesterone. The results obtained in biochemical analysis can conclude that the renal and liver activity and maternal-fetal exchange are normal in the different groups.O presente trabalho teve por objetivo quantificar a concentração dos constituintes bioquímicos e hormonal no líquido amniótico de fetos Nelore, oriundos de diferentes biotécnicas reprodutivas. Utilizaram-se 60 vacas gestantes divididas em: 1 – 20 vacas prenhes de bezerros oriundos de inseminação artificial convencional (IA); 2 – 20 vacas prenhes de bezerros oriundos de transferência de embriões (TE) e 3 – 20 vacas prenhes de bezerros oriundos de produção in vitro (PIV). Durante a fase de expulsão, o âmnion foi puncionado e colheram-se 15 mL de líquido amniótico. As determinações bioquímicas foram conduzidas por kits comerciais. A progesterona e a testosterona foram determinadas por meio de radioimunoensaio (RIA) em fase sólida. Empregou-se a análise de variância e teste de Tukey com 5% de significância. O teor de ureia e creatinina no G3 foi maior que nos outros grupos e significativo (p < 0,05) em relação ao G1. Para gamaglutamiltransferase (GGT), o G3 apresentou menor valor em relação ao G1 (p < 0,05). Os valores de glicose não diferiram entre os grupos. O cloreto apresentou maior concentração em G1 e G2 que no G3 (p < 0,05). O sódio e o potássio foram encontrados em maior quantidade no G 3 em relação aos demais grupos. Na análise hormonal não foi detectada diferença significativa para testosterona e progesterona. Pelos teores de ureia e creatinina, concluiu-se que a presença destes, sugere maturidade renal adequada, que a GGT (relacionada ao metabolismo animal) está elevada nos bezerros de produção in vitro e que os grupos tiveram a concentração de glicose adequada ao fornecimento de energia ao feto. Os resultados obtidos na análise bioquímica indicam que a atividade renal e hepática, além das trocas materno-fetal, está normal nos diferentes grupos.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Universidade Estadual Paulista, Departamento de Reprodução Animal e Radiologia Veterinária, Faculdade de Medicina Veterinária e Zootecnia de BotucatuUniversidade Estadual Paulista, Departamento de Clínica Veterinária, Faculdade de Medicina Veterinária e Zootecnia de BotucatuUniversidade Estadual Paulista (Unesp)Faculdades Integradas de OurinhosUniversidade Camilo Castelo Branco (UNICASTELO)Piagentini, Marcelo [UNESP]Prestes, Nereu Carlos [UNESP]Moya-Araujo, Carla FredrichsenAraujo, Gustavo Henrique MarquesTakahira, Regina Kiomi [UNESP]Oba, Eunice [UNESP]2016-07-07T12:34:30Z2016-07-07T12:34:30Z2012info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article205-209application/pdfhttp://dx.doi.org/10.7213/academica.7699Revista Acadêmica: Ciências Agrárias e Ambientais, v. 10, n. 2, p. 205-209, 2012.0103-989Xhttp://hdl.handle.net/11449/14057610.7213/academica.7699ISSN0103-989X-2012-10-02-205-209.pdf5946482110081687351782541017881392243805865576640000-0003-3323-4199Currículo Lattesreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengRevista Acadêmica: Ciências Agrárias e Ambientaisinfo:eu-repo/semantics/openAccess2024-09-09T14:01:41Zoai:repositorio.unesp.br:11449/140576Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-09T14:01:41Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Biochemical and hormonal profile of the amniotic fluid from Nelore calves conceived by different reproduction biotechnologies
Perfil bioquímico e hormonal do líquido amniótico de bezerros Nelore concebidos por diferentes biotecnologias
title Biochemical and hormonal profile of the amniotic fluid from Nelore calves conceived by different reproduction biotechnologies
spellingShingle Biochemical and hormonal profile of the amniotic fluid from Nelore calves conceived by different reproduction biotechnologies
Piagentini, Marcelo [UNESP]
Amniotic fluid
Laboratorial analyses
Nelore
Líquido amniótico
Análise laboratorial
Nelore
title_short Biochemical and hormonal profile of the amniotic fluid from Nelore calves conceived by different reproduction biotechnologies
title_full Biochemical and hormonal profile of the amniotic fluid from Nelore calves conceived by different reproduction biotechnologies
title_fullStr Biochemical and hormonal profile of the amniotic fluid from Nelore calves conceived by different reproduction biotechnologies
title_full_unstemmed Biochemical and hormonal profile of the amniotic fluid from Nelore calves conceived by different reproduction biotechnologies
title_sort Biochemical and hormonal profile of the amniotic fluid from Nelore calves conceived by different reproduction biotechnologies
author Piagentini, Marcelo [UNESP]
author_facet Piagentini, Marcelo [UNESP]
Prestes, Nereu Carlos [UNESP]
Moya-Araujo, Carla Fredrichsen
Araujo, Gustavo Henrique Marques
Takahira, Regina Kiomi [UNESP]
Oba, Eunice [UNESP]
author_role author
author2 Prestes, Nereu Carlos [UNESP]
Moya-Araujo, Carla Fredrichsen
Araujo, Gustavo Henrique Marques
Takahira, Regina Kiomi [UNESP]
Oba, Eunice [UNESP]
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Faculdades Integradas de Ourinhos
Universidade Camilo Castelo Branco (UNICASTELO)
dc.contributor.author.fl_str_mv Piagentini, Marcelo [UNESP]
Prestes, Nereu Carlos [UNESP]
Moya-Araujo, Carla Fredrichsen
Araujo, Gustavo Henrique Marques
Takahira, Regina Kiomi [UNESP]
Oba, Eunice [UNESP]
dc.subject.por.fl_str_mv Amniotic fluid
Laboratorial analyses
Nelore
Líquido amniótico
Análise laboratorial
Nelore
topic Amniotic fluid
Laboratorial analyses
Nelore
Líquido amniótico
Análise laboratorial
Nelore
description This study aimed to quantify biochemical constituents and hormonal concentrations in the amniotic fluid of Nelore calves conceived by different reproduction biotechnologies. Sixty animals were used and divided into: 1 – Twenty Nelore cows pregnant of calves obtained by artificial insemination; 2 – Twenty cows pregnant with Nelore calves obtained by embryo transference; 3 – Twenty cows pregnant with Nelore calves obtained by in vitro production. During the expulsion phase, the amnion was punctured and 15 mL of fluid were collected. The biochemical evaluation was performed with commercial kits in accordance to the manufacturer’s recommendations. It was used the radioimmunoassay to assess the progesterone and testosterone levels. The statistical analysis used was the variance analysis and Tukey (5% significance level). Mean urea and creatinine when comparing the three groups showed that group 3 had higher values of these two metabolites, suggesting that the kidney maturity is suitable. For gamma-glutamyltransferase, group 3 had a lower value compared to group 1. This enzyme is related to metabolism, may be inferred that the metabolic activity is high in animal production in vitro. The glucose values did not differ between the groups, suggests that the maternal-fetal exchanges were suitable for the power supply to the fetus. Lower chloride concentration was showed in group 3. Sodium and potassium were found in greater quantities in group 3 when compared to other groups. There was no significant difference for the testosterone and progesterone. The results obtained in biochemical analysis can conclude that the renal and liver activity and maternal-fetal exchange are normal in the different groups.
publishDate 2012
dc.date.none.fl_str_mv 2012
2016-07-07T12:34:30Z
2016-07-07T12:34:30Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.7213/academica.7699
Revista Acadêmica: Ciências Agrárias e Ambientais, v. 10, n. 2, p. 205-209, 2012.
0103-989X
http://hdl.handle.net/11449/140576
10.7213/academica.7699
ISSN0103-989X-2012-10-02-205-209.pdf
5946482110081687
3517825410178813
9224380586557664
0000-0003-3323-4199
url http://dx.doi.org/10.7213/academica.7699
http://hdl.handle.net/11449/140576
identifier_str_mv Revista Acadêmica: Ciências Agrárias e Ambientais, v. 10, n. 2, p. 205-209, 2012.
0103-989X
10.7213/academica.7699
ISSN0103-989X-2012-10-02-205-209.pdf
5946482110081687
3517825410178813
9224380586557664
0000-0003-3323-4199
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Revista Acadêmica: Ciências Agrárias e Ambientais
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 205-209
application/pdf
dc.source.none.fl_str_mv Currículo Lattes
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv repositoriounesp@unesp.br
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