Evaluation of alginate hydrogel encapsulated mesenchymal stem cell migration in horses
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.rvsc.2019.02.005 http://hdl.handle.net/11449/188775 |
Resumo: | Osteoarthritis is an incapacitating disease characterized by pain and a progressive decrease in joint mobility. The implantation of mesenchymal stem cells (MSCs) has shown promising results for its treatment. The challenge remains to keep the cells longer at the site of action, increasing their therapeutic potential. The aim of this study was to evaluate the effectiveness of the Qtracker® 655 nanocrystal marking on allogeneic synovial membrane (SM) MSCs, encapsulated in alginate hydrogel, evaluating the migration of these cells. The 10 radiocarpal joints were submitted to arthroscopic surgery (D0), divided into two groups. The chondral defect was treated according to the group: GA free-labelled MSC SM and GB labelled MSC SM microcapsules. Seven days after lesion induction and implantation of labelled cells, biopsies of the lesion site were performed in two animals, and fragments of SM and joint capsule also collected, which were frozen and later processed for fluorescence microscopy. The synovial fluid of the three animals was analyzed by flow cytometry three times – 3, 7 and 21 days after application. The cellular marking with the nanocrystals allowed the visualization of the cells in cartilage, synovial membrane, synovial fluid and articular capsule, but with a predilection for the synovial membrane and the lesion site was scarce. The labelled MSC SM in microcapsules were scarce in the synovial fluid and could be related to the small quantity of MSCs leaving the pores of the microcapsules, also favorable results, as the cells release paracrine effects acting for a long period until the cellular differentiation. |
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Evaluation of alginate hydrogel encapsulated mesenchymal stem cell migration in horsesCartilageHorseQuantum dotsScaffoldTissue engineeringTrackingOsteoarthritis is an incapacitating disease characterized by pain and a progressive decrease in joint mobility. The implantation of mesenchymal stem cells (MSCs) has shown promising results for its treatment. The challenge remains to keep the cells longer at the site of action, increasing their therapeutic potential. The aim of this study was to evaluate the effectiveness of the Qtracker® 655 nanocrystal marking on allogeneic synovial membrane (SM) MSCs, encapsulated in alginate hydrogel, evaluating the migration of these cells. The 10 radiocarpal joints were submitted to arthroscopic surgery (D0), divided into two groups. The chondral defect was treated according to the group: GA free-labelled MSC SM and GB labelled MSC SM microcapsules. Seven days after lesion induction and implantation of labelled cells, biopsies of the lesion site were performed in two animals, and fragments of SM and joint capsule also collected, which were frozen and later processed for fluorescence microscopy. The synovial fluid of the three animals was analyzed by flow cytometry three times – 3, 7 and 21 days after application. The cellular marking with the nanocrystals allowed the visualization of the cells in cartilage, synovial membrane, synovial fluid and articular capsule, but with a predilection for the synovial membrane and the lesion site was scarce. The labelled MSC SM in microcapsules were scarce in the synovial fluid and could be related to the small quantity of MSCs leaving the pores of the microcapsules, also favorable results, as the cells release paracrine effects acting for a long period until the cellular differentiation.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Department of Veterinary Surgery and Anesthesiology School of Veterinary Medicine and Animal Science São Paulo State University (UNESP)School of Medicine Blood Centre Division São Paulo State University (UNESP)Department of Veterinary Surgery and Anesthesiology School of Veterinary Medicine and Animal Science São Paulo State University (UNESP)School of Medicine Blood Centre Division São Paulo State University (UNESP)Universidade Estadual Paulista (Unesp)Santos, Vitor Hugo Dos [UNESP]Pfeifer, João Pedro Hübbe [UNESP]Souza, Jaqueline Brandão De [UNESP]Stievani, Fernanda De Castro [UNESP]Hussni, Carlos Alberto [UNESP]Golim, Marjorie De Assis [UNESP]Deffune, Elenice [UNESP]Alves, Ana Liz Garcia [UNESP]2019-10-06T16:18:51Z2019-10-06T16:18:51Z2019-06-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article38-45http://dx.doi.org/10.1016/j.rvsc.2019.02.005Research in Veterinary Science, v. 124, p. 38-45.1532-26610034-5288http://hdl.handle.net/11449/18877510.1016/j.rvsc.2019.02.0052-s2.0-85062079650Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengResearch in Veterinary Scienceinfo:eu-repo/semantics/openAccess2021-10-22T23:59:51Zoai:repositorio.unesp.br:11449/188775Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T19:25:58.165297Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Evaluation of alginate hydrogel encapsulated mesenchymal stem cell migration in horses |
title |
Evaluation of alginate hydrogel encapsulated mesenchymal stem cell migration in horses |
spellingShingle |
Evaluation of alginate hydrogel encapsulated mesenchymal stem cell migration in horses Santos, Vitor Hugo Dos [UNESP] Cartilage Horse Quantum dots Scaffold Tissue engineering Tracking |
title_short |
Evaluation of alginate hydrogel encapsulated mesenchymal stem cell migration in horses |
title_full |
Evaluation of alginate hydrogel encapsulated mesenchymal stem cell migration in horses |
title_fullStr |
Evaluation of alginate hydrogel encapsulated mesenchymal stem cell migration in horses |
title_full_unstemmed |
Evaluation of alginate hydrogel encapsulated mesenchymal stem cell migration in horses |
title_sort |
Evaluation of alginate hydrogel encapsulated mesenchymal stem cell migration in horses |
author |
Santos, Vitor Hugo Dos [UNESP] |
author_facet |
Santos, Vitor Hugo Dos [UNESP] Pfeifer, João Pedro Hübbe [UNESP] Souza, Jaqueline Brandão De [UNESP] Stievani, Fernanda De Castro [UNESP] Hussni, Carlos Alberto [UNESP] Golim, Marjorie De Assis [UNESP] Deffune, Elenice [UNESP] Alves, Ana Liz Garcia [UNESP] |
author_role |
author |
author2 |
Pfeifer, João Pedro Hübbe [UNESP] Souza, Jaqueline Brandão De [UNESP] Stievani, Fernanda De Castro [UNESP] Hussni, Carlos Alberto [UNESP] Golim, Marjorie De Assis [UNESP] Deffune, Elenice [UNESP] Alves, Ana Liz Garcia [UNESP] |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Santos, Vitor Hugo Dos [UNESP] Pfeifer, João Pedro Hübbe [UNESP] Souza, Jaqueline Brandão De [UNESP] Stievani, Fernanda De Castro [UNESP] Hussni, Carlos Alberto [UNESP] Golim, Marjorie De Assis [UNESP] Deffune, Elenice [UNESP] Alves, Ana Liz Garcia [UNESP] |
dc.subject.por.fl_str_mv |
Cartilage Horse Quantum dots Scaffold Tissue engineering Tracking |
topic |
Cartilage Horse Quantum dots Scaffold Tissue engineering Tracking |
description |
Osteoarthritis is an incapacitating disease characterized by pain and a progressive decrease in joint mobility. The implantation of mesenchymal stem cells (MSCs) has shown promising results for its treatment. The challenge remains to keep the cells longer at the site of action, increasing their therapeutic potential. The aim of this study was to evaluate the effectiveness of the Qtracker® 655 nanocrystal marking on allogeneic synovial membrane (SM) MSCs, encapsulated in alginate hydrogel, evaluating the migration of these cells. The 10 radiocarpal joints were submitted to arthroscopic surgery (D0), divided into two groups. The chondral defect was treated according to the group: GA free-labelled MSC SM and GB labelled MSC SM microcapsules. Seven days after lesion induction and implantation of labelled cells, biopsies of the lesion site were performed in two animals, and fragments of SM and joint capsule also collected, which were frozen and later processed for fluorescence microscopy. The synovial fluid of the three animals was analyzed by flow cytometry three times – 3, 7 and 21 days after application. The cellular marking with the nanocrystals allowed the visualization of the cells in cartilage, synovial membrane, synovial fluid and articular capsule, but with a predilection for the synovial membrane and the lesion site was scarce. The labelled MSC SM in microcapsules were scarce in the synovial fluid and could be related to the small quantity of MSCs leaving the pores of the microcapsules, also favorable results, as the cells release paracrine effects acting for a long period until the cellular differentiation. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-10-06T16:18:51Z 2019-10-06T16:18:51Z 2019-06-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.rvsc.2019.02.005 Research in Veterinary Science, v. 124, p. 38-45. 1532-2661 0034-5288 http://hdl.handle.net/11449/188775 10.1016/j.rvsc.2019.02.005 2-s2.0-85062079650 |
url |
http://dx.doi.org/10.1016/j.rvsc.2019.02.005 http://hdl.handle.net/11449/188775 |
identifier_str_mv |
Research in Veterinary Science, v. 124, p. 38-45. 1532-2661 0034-5288 10.1016/j.rvsc.2019.02.005 2-s2.0-85062079650 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Research in Veterinary Science |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
38-45 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129067708317696 |