Incidence and transplacental transmission of Neospora caninum in primiparous females from Bos indicus slaughtered in Presidente Pradente, São Paulo, Brazil
Autor(a) principal: | |
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Data de Publicação: | 2008 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://www.uel.br/revistas/uel/index.php/semagrarias/article/view/2829 http://hdl.handle.net/11449/13785 |
Resumo: | To produce an epidemiological map of neosporosis in Brazil and identify the types of transmission of this disease, the present study evaluated the occurrence of Neospora caninum in Nelore cattle (Bos indicus) in Presidente Prudent, west region of São Paulo state; its vertical transmission; and the early stage in which fetuses are infected. To achieve this, serum samples from 518 slaughtered pregnant heifers and their fetuses were tested by ELISA technique and fetal brain tissues subjected to PCR. One hundred and three heifers (19.88%) had antibodies to N. caninum, as well as 38 (36.8%) of fetuses from 4 months of gestation. The conventional PCR failed to detect N. caninum DNA. These findings show that neosporosis occurs in the area studied and that it may be transmitted the transplacental route, althought N. caninum had not detected in brain tissue from non-aborted fetuses. The use of nested PCR it would be applied to increase the sensitivy of test. |
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Incidence and transplacental transmission of Neospora caninum in primiparous females from Bos indicus slaughtered in Presidente Pradente, São Paulo, BrazilepidemiologycattleNeospora caninumTo produce an epidemiological map of neosporosis in Brazil and identify the types of transmission of this disease, the present study evaluated the occurrence of Neospora caninum in Nelore cattle (Bos indicus) in Presidente Prudent, west region of São Paulo state; its vertical transmission; and the early stage in which fetuses are infected. To achieve this, serum samples from 518 slaughtered pregnant heifers and their fetuses were tested by ELISA technique and fetal brain tissues subjected to PCR. One hundred and three heifers (19.88%) had antibodies to N. caninum, as well as 38 (36.8%) of fetuses from 4 months of gestation. The conventional PCR failed to detect N. caninum DNA. These findings show that neosporosis occurs in the area studied and that it may be transmitted the transplacental route, althought N. caninum had not detected in brain tissue from non-aborted fetuses. The use of nested PCR it would be applied to increase the sensitivy of test.Univ Estadual Paulista UNESP, BR-18618000 Botucatu, SP, BrazilInst Biol, São Paulo, BrazilUniv Oeste Paulista UNOESTE, Presidente Prudente, SP, BrazilUniv Estadual Paulista UNESP, BR-18618000 Botucatu, SP, BrazilUniversidade Estadual de Londrina (UEL)Universidade Estadual Paulista (Unesp)Inst BiolUniversidade do Oeste Paulista (UNOESTE)Vianna, Luis Carlos [UNESP]Sartor, Izidoro Francisco [UNESP]Pituco, Edviges Maristela.Okuda, Liria HiromiCamargo, Clarice NevesKronka, Sergio do Nascimento2014-05-20T13:39:43Z2014-05-20T13:39:43Z2008-04-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article387-392application/pdfhttp://www.uel.br/revistas/uel/index.php/semagrarias/article/view/2829Semina-ciencias Agrarias. Londrina: Universidade Estadual de Londrina (UEL), v. 29, n. 2, p. 387-392, 2008.1676-546Xhttp://hdl.handle.net/11449/13785WOS:000257475100016WOS000257475100016.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengSemina: Ciências Agrárias0.349info:eu-repo/semantics/openAccess2023-10-14T06:06:39Zoai:repositorio.unesp.br:11449/13785Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-10-14T06:06:39Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Incidence and transplacental transmission of Neospora caninum in primiparous females from Bos indicus slaughtered in Presidente Pradente, São Paulo, Brazil |
title |
Incidence and transplacental transmission of Neospora caninum in primiparous females from Bos indicus slaughtered in Presidente Pradente, São Paulo, Brazil |
spellingShingle |
Incidence and transplacental transmission of Neospora caninum in primiparous females from Bos indicus slaughtered in Presidente Pradente, São Paulo, Brazil Vianna, Luis Carlos [UNESP] epidemiology cattle Neospora caninum |
title_short |
Incidence and transplacental transmission of Neospora caninum in primiparous females from Bos indicus slaughtered in Presidente Pradente, São Paulo, Brazil |
title_full |
Incidence and transplacental transmission of Neospora caninum in primiparous females from Bos indicus slaughtered in Presidente Pradente, São Paulo, Brazil |
title_fullStr |
Incidence and transplacental transmission of Neospora caninum in primiparous females from Bos indicus slaughtered in Presidente Pradente, São Paulo, Brazil |
title_full_unstemmed |
Incidence and transplacental transmission of Neospora caninum in primiparous females from Bos indicus slaughtered in Presidente Pradente, São Paulo, Brazil |
title_sort |
Incidence and transplacental transmission of Neospora caninum in primiparous females from Bos indicus slaughtered in Presidente Pradente, São Paulo, Brazil |
author |
Vianna, Luis Carlos [UNESP] |
author_facet |
Vianna, Luis Carlos [UNESP] Sartor, Izidoro Francisco [UNESP] Pituco, Edviges Maristela. Okuda, Liria Hiromi Camargo, Clarice Neves Kronka, Sergio do Nascimento |
author_role |
author |
author2 |
Sartor, Izidoro Francisco [UNESP] Pituco, Edviges Maristela. Okuda, Liria Hiromi Camargo, Clarice Neves Kronka, Sergio do Nascimento |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Inst Biol Universidade do Oeste Paulista (UNOESTE) |
dc.contributor.author.fl_str_mv |
Vianna, Luis Carlos [UNESP] Sartor, Izidoro Francisco [UNESP] Pituco, Edviges Maristela. Okuda, Liria Hiromi Camargo, Clarice Neves Kronka, Sergio do Nascimento |
dc.subject.por.fl_str_mv |
epidemiology cattle Neospora caninum |
topic |
epidemiology cattle Neospora caninum |
description |
To produce an epidemiological map of neosporosis in Brazil and identify the types of transmission of this disease, the present study evaluated the occurrence of Neospora caninum in Nelore cattle (Bos indicus) in Presidente Prudent, west region of São Paulo state; its vertical transmission; and the early stage in which fetuses are infected. To achieve this, serum samples from 518 slaughtered pregnant heifers and their fetuses were tested by ELISA technique and fetal brain tissues subjected to PCR. One hundred and three heifers (19.88%) had antibodies to N. caninum, as well as 38 (36.8%) of fetuses from 4 months of gestation. The conventional PCR failed to detect N. caninum DNA. These findings show that neosporosis occurs in the area studied and that it may be transmitted the transplacental route, althought N. caninum had not detected in brain tissue from non-aborted fetuses. The use of nested PCR it would be applied to increase the sensitivy of test. |
publishDate |
2008 |
dc.date.none.fl_str_mv |
2008-04-01 2014-05-20T13:39:43Z 2014-05-20T13:39:43Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.uel.br/revistas/uel/index.php/semagrarias/article/view/2829 Semina-ciencias Agrarias. Londrina: Universidade Estadual de Londrina (UEL), v. 29, n. 2, p. 387-392, 2008. 1676-546X http://hdl.handle.net/11449/13785 WOS:000257475100016 WOS000257475100016.pdf |
url |
http://www.uel.br/revistas/uel/index.php/semagrarias/article/view/2829 http://hdl.handle.net/11449/13785 |
identifier_str_mv |
Semina-ciencias Agrarias. Londrina: Universidade Estadual de Londrina (UEL), v. 29, n. 2, p. 387-392, 2008. 1676-546X WOS:000257475100016 WOS000257475100016.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Semina: Ciências Agrárias 0.349 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
387-392 application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Estadual de Londrina (UEL) |
publisher.none.fl_str_mv |
Universidade Estadual de Londrina (UEL) |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1797789392723509248 |