AN ULTRASTRUCTURAL-STUDY ON THE ENDOCRINE PANCREAS OF CAIMAN-LATIROSTRIS, WITH SPECIAL REFERENCE TO PANCREATIC MOTILIN CELLS
Autor(a) principal: | |
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Data de Publicação: | 1991 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1679/aohc.54.349 http://hdl.handle.net/11449/33388 |
Resumo: | Pancreatic endocrine cells of Caiman latirostris were investigated by electron microscopy using conventional and immunocytochemical methods. Ultrastructurally, four types of endocrine cells were classified according to the morphology of their secretory granules. Three types of endocrine cells were identified as either glucagon, insulin or somatostatin cells by the presence of such characteristic granules well established in mammals. The remaining endocrine cell type could not be classified by its ultrastructural features alone.Immunocytochemical observations confirmed the ultrastructural classification of glucagon, insulin and somatostatin cells. In addition, endocrine cells immunoreactive for either pancreatic polypeptide (PP) or motilin were identified. Morphometric analysis of PP- and motilin-immunoreactive granules demonstrated that they were the most polymorphous and smallest granules among the pancreatic endocrine cell granules. Although both PP and motilin granules closely resemble each other, motilin granules were smaller in size and more spherical in shape than PP granules. |
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Repositório Institucional da UNESP |
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AN ULTRASTRUCTURAL-STUDY ON THE ENDOCRINE PANCREAS OF CAIMAN-LATIROSTRIS, WITH SPECIAL REFERENCE TO PANCREATIC MOTILIN CELLSPancreatic endocrine cells of Caiman latirostris were investigated by electron microscopy using conventional and immunocytochemical methods. Ultrastructurally, four types of endocrine cells were classified according to the morphology of their secretory granules. Three types of endocrine cells were identified as either glucagon, insulin or somatostatin cells by the presence of such characteristic granules well established in mammals. The remaining endocrine cell type could not be classified by its ultrastructural features alone.Immunocytochemical observations confirmed the ultrastructural classification of glucagon, insulin and somatostatin cells. In addition, endocrine cells immunoreactive for either pancreatic polypeptide (PP) or motilin were identified. Morphometric analysis of PP- and motilin-immunoreactive granules demonstrated that they were the most polymorphous and smallest granules among the pancreatic endocrine cell granules. Although both PP and motilin granules closely resemble each other, motilin granules were smaller in size and more spherical in shape than PP granules.OBIHIRO UNIV AGR & VET MED,DEPT VET ANAT,NISHI-2,INADA CHO,OBIHIRO,HOKKAIDO 080,JAPANSAO PAULO STATE UNIV,DEPT MORPHOL,BOTUCATU,SP,BRAZILSAO PAULO STATE UNIV,DEPT MORPHOL,BOTUCATU,SP,BRAZILJapan Soc Histol Documentation Niigata Univ Medical SchoolOBIHIRO UNIV AGR & VET MEDUniversidade Estadual Paulista (Unesp)Ono, K.Yamada, J.DPAI, VKitamura, N.Gregorio, E. A.Yamashita, T.Campos, VJM2014-05-20T15:22:24Z2014-05-20T15:22:24Z1991-07-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article349-357application/pdfhttp://dx.doi.org/10.1679/aohc.54.349Archives of Histology and Cytology. Niigata: Japan Soc Histol Documentation Niigata Univ Medical School, v. 54, n. 3, p. 349-357, 1991.0914-9465http://hdl.handle.net/11449/3338810.1679/aohc.54.349WOS:A1991GA03100010WOSA1991GA03100010.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengArchives of Histology and Cytology0,105info:eu-repo/semantics/openAccess2023-11-02T06:07:19Zoai:repositorio.unesp.br:11449/33388Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T16:42:16.126013Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
AN ULTRASTRUCTURAL-STUDY ON THE ENDOCRINE PANCREAS OF CAIMAN-LATIROSTRIS, WITH SPECIAL REFERENCE TO PANCREATIC MOTILIN CELLS |
title |
AN ULTRASTRUCTURAL-STUDY ON THE ENDOCRINE PANCREAS OF CAIMAN-LATIROSTRIS, WITH SPECIAL REFERENCE TO PANCREATIC MOTILIN CELLS |
spellingShingle |
AN ULTRASTRUCTURAL-STUDY ON THE ENDOCRINE PANCREAS OF CAIMAN-LATIROSTRIS, WITH SPECIAL REFERENCE TO PANCREATIC MOTILIN CELLS Ono, K. |
title_short |
AN ULTRASTRUCTURAL-STUDY ON THE ENDOCRINE PANCREAS OF CAIMAN-LATIROSTRIS, WITH SPECIAL REFERENCE TO PANCREATIC MOTILIN CELLS |
title_full |
AN ULTRASTRUCTURAL-STUDY ON THE ENDOCRINE PANCREAS OF CAIMAN-LATIROSTRIS, WITH SPECIAL REFERENCE TO PANCREATIC MOTILIN CELLS |
title_fullStr |
AN ULTRASTRUCTURAL-STUDY ON THE ENDOCRINE PANCREAS OF CAIMAN-LATIROSTRIS, WITH SPECIAL REFERENCE TO PANCREATIC MOTILIN CELLS |
title_full_unstemmed |
AN ULTRASTRUCTURAL-STUDY ON THE ENDOCRINE PANCREAS OF CAIMAN-LATIROSTRIS, WITH SPECIAL REFERENCE TO PANCREATIC MOTILIN CELLS |
title_sort |
AN ULTRASTRUCTURAL-STUDY ON THE ENDOCRINE PANCREAS OF CAIMAN-LATIROSTRIS, WITH SPECIAL REFERENCE TO PANCREATIC MOTILIN CELLS |
author |
Ono, K. |
author_facet |
Ono, K. Yamada, J. DPAI, V Kitamura, N. Gregorio, E. A. Yamashita, T. Campos, VJM |
author_role |
author |
author2 |
Yamada, J. DPAI, V Kitamura, N. Gregorio, E. A. Yamashita, T. Campos, VJM |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
OBIHIRO UNIV AGR & VET MED Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Ono, K. Yamada, J. DPAI, V Kitamura, N. Gregorio, E. A. Yamashita, T. Campos, VJM |
description |
Pancreatic endocrine cells of Caiman latirostris were investigated by electron microscopy using conventional and immunocytochemical methods. Ultrastructurally, four types of endocrine cells were classified according to the morphology of their secretory granules. Three types of endocrine cells were identified as either glucagon, insulin or somatostatin cells by the presence of such characteristic granules well established in mammals. The remaining endocrine cell type could not be classified by its ultrastructural features alone.Immunocytochemical observations confirmed the ultrastructural classification of glucagon, insulin and somatostatin cells. In addition, endocrine cells immunoreactive for either pancreatic polypeptide (PP) or motilin were identified. Morphometric analysis of PP- and motilin-immunoreactive granules demonstrated that they were the most polymorphous and smallest granules among the pancreatic endocrine cell granules. Although both PP and motilin granules closely resemble each other, motilin granules were smaller in size and more spherical in shape than PP granules. |
publishDate |
1991 |
dc.date.none.fl_str_mv |
1991-07-01 2014-05-20T15:22:24Z 2014-05-20T15:22:24Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1679/aohc.54.349 Archives of Histology and Cytology. Niigata: Japan Soc Histol Documentation Niigata Univ Medical School, v. 54, n. 3, p. 349-357, 1991. 0914-9465 http://hdl.handle.net/11449/33388 10.1679/aohc.54.349 WOS:A1991GA03100010 WOSA1991GA03100010.pdf |
url |
http://dx.doi.org/10.1679/aohc.54.349 http://hdl.handle.net/11449/33388 |
identifier_str_mv |
Archives of Histology and Cytology. Niigata: Japan Soc Histol Documentation Niigata Univ Medical School, v. 54, n. 3, p. 349-357, 1991. 0914-9465 10.1679/aohc.54.349 WOS:A1991GA03100010 WOSA1991GA03100010.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Archives of Histology and Cytology 0,105 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
349-357 application/pdf |
dc.publisher.none.fl_str_mv |
Japan Soc Histol Documentation Niigata Univ Medical School |
publisher.none.fl_str_mv |
Japan Soc Histol Documentation Niigata Univ Medical School |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128688982589440 |