Subconjunctival and topical application of recombinant tissue plasminogen activator in rabbits

Detalhes bibliográficos
Autor(a) principal: De Abreu Reggi, José Ricardo
Data de Publicação: 2015
Outros Autores: Hida, Richard Yudi, Hida, Milton Massato [UNESP], Nishiwaki-Dantas, Maria Cristina, Suzuki, Hisashi
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.5935/0004-2749.20150003
http://hdl.handle.net/11449/177306
Resumo: Purpose: To quantify fibrin degradation products after topical and subconjunctival administration of recombinant tissue plasminogen activator in rabbits. Methods: Fibrin formation was induced in the anterior chamber in 25 rabbits. Subsequently, five rabbits received an injection of r-TPA (positive control) in the anterior chamber, another 10 received a subconjunctival injection of r-TPA, and the remaining 10 received instillations of topical r-TPA. Afterwards, samples of aqueous humor were collected and semi-quantitative analysis of fibrin degradation products (FDP) was performed. Results: No statistical differences were noted between the treatment and control groups at any time point. Fibrin degradation products semi-quantification showed statistical improvement in the control group and the subconjunctival group. Conclusion: Fibrin degradation products were observed in the anterior chamber after subconjunctival administration of r-TPA. However, it was probably not sufficient to cause fibrin degradation. Topical r-TPA did not effectively absorb anterior chamber fibrin.
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spelling Subconjunctival and topical application of recombinant tissue plasminogen activator in rabbitsAnimalsAnterior chamberHyphemaInflammationPostoperative complicationsRabbitsTissue plasminogen activatorPurpose: To quantify fibrin degradation products after topical and subconjunctival administration of recombinant tissue plasminogen activator in rabbits. Methods: Fibrin formation was induced in the anterior chamber in 25 rabbits. Subsequently, five rabbits received an injection of r-TPA (positive control) in the anterior chamber, another 10 received a subconjunctival injection of r-TPA, and the remaining 10 received instillations of topical r-TPA. Afterwards, samples of aqueous humor were collected and semi-quantitative analysis of fibrin degradation products (FDP) was performed. Results: No statistical differences were noted between the treatment and control groups at any time point. Fibrin degradation products semi-quantification showed statistical improvement in the control group and the subconjunctival group. Conclusion: Fibrin degradation products were observed in the anterior chamber after subconjunctival administration of r-TPA. However, it was probably not sufficient to cause fibrin degradation. Topical r-TPA did not effectively absorb anterior chamber fibrin.Department of Ophthalmology, Santa Casa de São PauloDepartment of Ophthalmology, University of São Paulo (USP)Department of Ophthalmology, Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP)Department of Ophthalmology, Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP)Universidade de São Paulo (USP)Universidade Estadual Paulista (Unesp)De Abreu Reggi, José RicardoHida, Richard YudiHida, Milton Massato [UNESP]Nishiwaki-Dantas, Maria CristinaSuzuki, Hisashi2018-12-11T17:24:53Z2018-12-11T17:24:53Z2015-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article6-9application/pdfhttp://dx.doi.org/10.5935/0004-2749.20150003Arquivos Brasileiros de Oftalmologia, v. 78, n. 1, p. 6-9, 2015.1678-29250004-2749http://hdl.handle.net/11449/17730610.5935/0004-2749.201500032-s2.0-849233985142-s2.0-84923398514.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengArquivos Brasileiros de Oftalmologia0,518info:eu-repo/semantics/openAccess2024-08-16T18:44:32Zoai:repositorio.unesp.br:11449/177306Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-16T18:44:32Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Subconjunctival and topical application of recombinant tissue plasminogen activator in rabbits
title Subconjunctival and topical application of recombinant tissue plasminogen activator in rabbits
spellingShingle Subconjunctival and topical application of recombinant tissue plasminogen activator in rabbits
De Abreu Reggi, José Ricardo
Animals
Anterior chamber
Hyphema
Inflammation
Postoperative complications
Rabbits
Tissue plasminogen activator
title_short Subconjunctival and topical application of recombinant tissue plasminogen activator in rabbits
title_full Subconjunctival and topical application of recombinant tissue plasminogen activator in rabbits
title_fullStr Subconjunctival and topical application of recombinant tissue plasminogen activator in rabbits
title_full_unstemmed Subconjunctival and topical application of recombinant tissue plasminogen activator in rabbits
title_sort Subconjunctival and topical application of recombinant tissue plasminogen activator in rabbits
author De Abreu Reggi, José Ricardo
author_facet De Abreu Reggi, José Ricardo
Hida, Richard Yudi
Hida, Milton Massato [UNESP]
Nishiwaki-Dantas, Maria Cristina
Suzuki, Hisashi
author_role author
author2 Hida, Richard Yudi
Hida, Milton Massato [UNESP]
Nishiwaki-Dantas, Maria Cristina
Suzuki, Hisashi
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade de São Paulo (USP)
Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv De Abreu Reggi, José Ricardo
Hida, Richard Yudi
Hida, Milton Massato [UNESP]
Nishiwaki-Dantas, Maria Cristina
Suzuki, Hisashi
dc.subject.por.fl_str_mv Animals
Anterior chamber
Hyphema
Inflammation
Postoperative complications
Rabbits
Tissue plasminogen activator
topic Animals
Anterior chamber
Hyphema
Inflammation
Postoperative complications
Rabbits
Tissue plasminogen activator
description Purpose: To quantify fibrin degradation products after topical and subconjunctival administration of recombinant tissue plasminogen activator in rabbits. Methods: Fibrin formation was induced in the anterior chamber in 25 rabbits. Subsequently, five rabbits received an injection of r-TPA (positive control) in the anterior chamber, another 10 received a subconjunctival injection of r-TPA, and the remaining 10 received instillations of topical r-TPA. Afterwards, samples of aqueous humor were collected and semi-quantitative analysis of fibrin degradation products (FDP) was performed. Results: No statistical differences were noted between the treatment and control groups at any time point. Fibrin degradation products semi-quantification showed statistical improvement in the control group and the subconjunctival group. Conclusion: Fibrin degradation products were observed in the anterior chamber after subconjunctival administration of r-TPA. However, it was probably not sufficient to cause fibrin degradation. Topical r-TPA did not effectively absorb anterior chamber fibrin.
publishDate 2015
dc.date.none.fl_str_mv 2015-01-01
2018-12-11T17:24:53Z
2018-12-11T17:24:53Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.5935/0004-2749.20150003
Arquivos Brasileiros de Oftalmologia, v. 78, n. 1, p. 6-9, 2015.
1678-2925
0004-2749
http://hdl.handle.net/11449/177306
10.5935/0004-2749.20150003
2-s2.0-84923398514
2-s2.0-84923398514.pdf
url http://dx.doi.org/10.5935/0004-2749.20150003
http://hdl.handle.net/11449/177306
identifier_str_mv Arquivos Brasileiros de Oftalmologia, v. 78, n. 1, p. 6-9, 2015.
1678-2925
0004-2749
10.5935/0004-2749.20150003
2-s2.0-84923398514
2-s2.0-84923398514.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Arquivos Brasileiros de Oftalmologia
0,518
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 6-9
application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1808128202921476096

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