Chloroquine is therapeutic in murine experimental model of paracoccidioidomycosis
Autor(a) principal: | |
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Data de Publicação: | 2007 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1111/j.1574-695X.2007.00243.x http://hdl.handle.net/11449/31770 |
Resumo: | Chloroquine, due to its basic properties, has been shown to prevent the release of iron from holotransferrin, thereby interfering with normal iron metabolism in a variety of cell types. We have studied the effects of chloroquine on the evolution of experimental paracoccidioidomycosis by evaluating the viable fungal recovery from lung, liver and spleen from infected mice and H2O2, NO production, tumor necrosis factor-alpha (TNF-alpha), interleukin (IL)-6, IL-10 levels and transferrin receptor (TfR) expression from uninfected and infected peritoneal macrophages. Chloroquine caused a significant decrease in the viable fungal recovery from all organs tested, during all periods of evaluation. Peritoneal macrophages from chloroquine-treated infected mice showed higher H2O2 production and TfR expression, and decreased levels of NO, endogenous and stimulated-TNF-alpha, IL-6 and IL-10 during the three evaluated periods. However, despite its suppressor effects on the macrophage function, the chloroquine therapeutic effect upon murine paracoccidioidomycosis was probably due to its effect on iron metabolism, blocking iron uptake by cells, and consequently restricting iron to fungus growth and survival. |
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Chloroquine is therapeutic in murine experimental model of paracoccidioidomycosisParacoccidioides brasiliensischloroquineironhydrogen peroxidenitric oxidecytokinesChloroquine, due to its basic properties, has been shown to prevent the release of iron from holotransferrin, thereby interfering with normal iron metabolism in a variety of cell types. We have studied the effects of chloroquine on the evolution of experimental paracoccidioidomycosis by evaluating the viable fungal recovery from lung, liver and spleen from infected mice and H2O2, NO production, tumor necrosis factor-alpha (TNF-alpha), interleukin (IL)-6, IL-10 levels and transferrin receptor (TfR) expression from uninfected and infected peritoneal macrophages. Chloroquine caused a significant decrease in the viable fungal recovery from all organs tested, during all periods of evaluation. Peritoneal macrophages from chloroquine-treated infected mice showed higher H2O2 production and TfR expression, and decreased levels of NO, endogenous and stimulated-TNF-alpha, IL-6 and IL-10 during the three evaluated periods. However, despite its suppressor effects on the macrophage function, the chloroquine therapeutic effect upon murine paracoccidioidomycosis was probably due to its effect on iron metabolism, blocking iron uptake by cells, and consequently restricting iron to fungus growth and survival.São Paulo State Univ, Dept Microbiol & Immunol, Biosci Inst, São Paulo, BrazilSch Med, Dept Trop Dis, São Paulo, BrazilSão Paulo State Univ, Sch Med, Botucatu Blood Ctr, São Paulo, BrazilSão Paulo State Univ, Dept Microbiol & Immunol, Biosci Inst, São Paulo, BrazilSão Paulo State Univ, Sch Med, Botucatu Blood Ctr, São Paulo, BrazilBlackwell PublishingUniversidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)Dias-Melicio, Luciane AlarcaoCalvi, Sueli AparecidaBordon, Ana PaulaGolim, Marjorie A.Serrao Peracoil, Maria TerezinhaVictoriano Campos Soares, Angela Maria2014-05-20T15:20:29Z2014-05-20T15:20:29Z2007-06-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article133-143application/pdfhttp://dx.doi.org/10.1111/j.1574-695X.2007.00243.xFems Immunology and Medical Microbiology. Oxford: Blackwell Publishing, v. 50, n. 1, p. 133-143, 2007.0928-8244http://hdl.handle.net/11449/3177010.1111/j.1574-695X.2007.00243.xWOS:000246579000015WOS000246579000015.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengFEMS Immunology and Medical Microbiologyinfo:eu-repo/semantics/openAccess2023-12-04T06:18:29Zoai:repositorio.unesp.br:11449/31770Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-12-04T06:18:29Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Chloroquine is therapeutic in murine experimental model of paracoccidioidomycosis |
title |
Chloroquine is therapeutic in murine experimental model of paracoccidioidomycosis |
spellingShingle |
Chloroquine is therapeutic in murine experimental model of paracoccidioidomycosis Dias-Melicio, Luciane Alarcao Paracoccidioides brasiliensis chloroquine iron hydrogen peroxide nitric oxide cytokines |
title_short |
Chloroquine is therapeutic in murine experimental model of paracoccidioidomycosis |
title_full |
Chloroquine is therapeutic in murine experimental model of paracoccidioidomycosis |
title_fullStr |
Chloroquine is therapeutic in murine experimental model of paracoccidioidomycosis |
title_full_unstemmed |
Chloroquine is therapeutic in murine experimental model of paracoccidioidomycosis |
title_sort |
Chloroquine is therapeutic in murine experimental model of paracoccidioidomycosis |
author |
Dias-Melicio, Luciane Alarcao |
author_facet |
Dias-Melicio, Luciane Alarcao Calvi, Sueli Aparecida Bordon, Ana Paula Golim, Marjorie A. Serrao Peracoil, Maria Terezinha Victoriano Campos Soares, Angela Maria |
author_role |
author |
author2 |
Calvi, Sueli Aparecida Bordon, Ana Paula Golim, Marjorie A. Serrao Peracoil, Maria Terezinha Victoriano Campos Soares, Angela Maria |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Dias-Melicio, Luciane Alarcao Calvi, Sueli Aparecida Bordon, Ana Paula Golim, Marjorie A. Serrao Peracoil, Maria Terezinha Victoriano Campos Soares, Angela Maria |
dc.subject.por.fl_str_mv |
Paracoccidioides brasiliensis chloroquine iron hydrogen peroxide nitric oxide cytokines |
topic |
Paracoccidioides brasiliensis chloroquine iron hydrogen peroxide nitric oxide cytokines |
description |
Chloroquine, due to its basic properties, has been shown to prevent the release of iron from holotransferrin, thereby interfering with normal iron metabolism in a variety of cell types. We have studied the effects of chloroquine on the evolution of experimental paracoccidioidomycosis by evaluating the viable fungal recovery from lung, liver and spleen from infected mice and H2O2, NO production, tumor necrosis factor-alpha (TNF-alpha), interleukin (IL)-6, IL-10 levels and transferrin receptor (TfR) expression from uninfected and infected peritoneal macrophages. Chloroquine caused a significant decrease in the viable fungal recovery from all organs tested, during all periods of evaluation. Peritoneal macrophages from chloroquine-treated infected mice showed higher H2O2 production and TfR expression, and decreased levels of NO, endogenous and stimulated-TNF-alpha, IL-6 and IL-10 during the three evaluated periods. However, despite its suppressor effects on the macrophage function, the chloroquine therapeutic effect upon murine paracoccidioidomycosis was probably due to its effect on iron metabolism, blocking iron uptake by cells, and consequently restricting iron to fungus growth and survival. |
publishDate |
2007 |
dc.date.none.fl_str_mv |
2007-06-01 2014-05-20T15:20:29Z 2014-05-20T15:20:29Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1111/j.1574-695X.2007.00243.x Fems Immunology and Medical Microbiology. Oxford: Blackwell Publishing, v. 50, n. 1, p. 133-143, 2007. 0928-8244 http://hdl.handle.net/11449/31770 10.1111/j.1574-695X.2007.00243.x WOS:000246579000015 WOS000246579000015.pdf |
url |
http://dx.doi.org/10.1111/j.1574-695X.2007.00243.x http://hdl.handle.net/11449/31770 |
identifier_str_mv |
Fems Immunology and Medical Microbiology. Oxford: Blackwell Publishing, v. 50, n. 1, p. 133-143, 2007. 0928-8244 10.1111/j.1574-695X.2007.00243.x WOS:000246579000015 WOS000246579000015.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
FEMS Immunology and Medical Microbiology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
133-143 application/pdf |
dc.publisher.none.fl_str_mv |
Blackwell Publishing |
publisher.none.fl_str_mv |
Blackwell Publishing |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1803046800393764864 |