Determination of carbaryl by ELISA (enzyme-linked immunosorbent assay) and HPLC with photodiode array detection

Detalhes bibliográficos
Autor(a) principal: Toscano, IAS
Data de Publicação: 2000
Outros Autores: Ribeiro, M. L., Rocha, J. C., Nunes, G. S., Barcelo, D.
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://hdl.handle.net/11449/194551
Resumo: ELISAs have been applied to pesticide residue analysis due to their high sensitivity and selectivity. However, some ELISAs performance may be affected by matrix components. In this work, ELISA for carbaryl in water samples was checked for interference by naturally occurring fulvic acids. The results suggested that the high fulvic acid concentration (greater than or equal to 30 mg L-1) and acidic pH conditions (pH 4.0) interfere with the signal detection decreasing the method sensitivity. A dilution of the samples and adjust to pH 8.0 are appropriate to minimize the matrix interferences in the ELISA method. Good correlation between ELISA and HPLC-DAD results was observed.
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spelling Determination of carbaryl by ELISA (enzyme-linked immunosorbent assay) and HPLC with photodiode array detectionELISAcarbarylfulvic acidsELISAs have been applied to pesticide residue analysis due to their high sensitivity and selectivity. However, some ELISAs performance may be affected by matrix components. In this work, ELISA for carbaryl in water samples was checked for interference by naturally occurring fulvic acids. The results suggested that the high fulvic acid concentration (greater than or equal to 30 mg L-1) and acidic pH conditions (pH 4.0) interfere with the signal detection decreasing the method sensitivity. A dilution of the samples and adjust to pH 8.0 are appropriate to minimize the matrix interferences in the ELISA method. Good correlation between ELISA and HPLC-DAD results was observed.Univ Estadual Paulista, Inst Quim, BR-14800900 Araraquara, SP, BrazilUniv Fed Maranhao, Dept Quim Tecnol, BR-65080040 Sao Luis, MA, BrazilCtr Invest & Desarrollo, Dept Quim Ambiental, Barcelona 08035, SpainUniv Estadual Paulista, Inst Quim, BR-14800900 Araraquara, SP, BrazilSoc Brasileira QuimicaUniversidade Estadual Paulista (Unesp)Univ Fed MaranhaoCtr Invest & DesarrolloToscano, IASRibeiro, M. L.Rocha, J. C.Nunes, G. S.Barcelo, D.2020-12-10T16:29:46Z2020-12-10T16:29:46Z2000-07-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article466-471Quimica Nova. Sao Paulo: Soc Brasileira Quimica, v. 23, n. 4, p. 466-471, 2000.0100-4042http://hdl.handle.net/11449/194551WOS:000088609600006Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPporQuimica Novainfo:eu-repo/semantics/openAccess2021-10-22T17:20:05Zoai:repositorio.unesp.br:11449/194551Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T15:29:44.418748Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Determination of carbaryl by ELISA (enzyme-linked immunosorbent assay) and HPLC with photodiode array detection
title Determination of carbaryl by ELISA (enzyme-linked immunosorbent assay) and HPLC with photodiode array detection
spellingShingle Determination of carbaryl by ELISA (enzyme-linked immunosorbent assay) and HPLC with photodiode array detection
Toscano, IAS
ELISA
carbaryl
fulvic acids
title_short Determination of carbaryl by ELISA (enzyme-linked immunosorbent assay) and HPLC with photodiode array detection
title_full Determination of carbaryl by ELISA (enzyme-linked immunosorbent assay) and HPLC with photodiode array detection
title_fullStr Determination of carbaryl by ELISA (enzyme-linked immunosorbent assay) and HPLC with photodiode array detection
title_full_unstemmed Determination of carbaryl by ELISA (enzyme-linked immunosorbent assay) and HPLC with photodiode array detection
title_sort Determination of carbaryl by ELISA (enzyme-linked immunosorbent assay) and HPLC with photodiode array detection
author Toscano, IAS
author_facet Toscano, IAS
Ribeiro, M. L.
Rocha, J. C.
Nunes, G. S.
Barcelo, D.
author_role author
author2 Ribeiro, M. L.
Rocha, J. C.
Nunes, G. S.
Barcelo, D.
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Univ Fed Maranhao
Ctr Invest & Desarrollo
dc.contributor.author.fl_str_mv Toscano, IAS
Ribeiro, M. L.
Rocha, J. C.
Nunes, G. S.
Barcelo, D.
dc.subject.por.fl_str_mv ELISA
carbaryl
fulvic acids
topic ELISA
carbaryl
fulvic acids
description ELISAs have been applied to pesticide residue analysis due to their high sensitivity and selectivity. However, some ELISAs performance may be affected by matrix components. In this work, ELISA for carbaryl in water samples was checked for interference by naturally occurring fulvic acids. The results suggested that the high fulvic acid concentration (greater than or equal to 30 mg L-1) and acidic pH conditions (pH 4.0) interfere with the signal detection decreasing the method sensitivity. A dilution of the samples and adjust to pH 8.0 are appropriate to minimize the matrix interferences in the ELISA method. Good correlation between ELISA and HPLC-DAD results was observed.
publishDate 2000
dc.date.none.fl_str_mv 2000-07-01
2020-12-10T16:29:46Z
2020-12-10T16:29:46Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv Quimica Nova. Sao Paulo: Soc Brasileira Quimica, v. 23, n. 4, p. 466-471, 2000.
0100-4042
http://hdl.handle.net/11449/194551
WOS:000088609600006
identifier_str_mv Quimica Nova. Sao Paulo: Soc Brasileira Quimica, v. 23, n. 4, p. 466-471, 2000.
0100-4042
WOS:000088609600006
url http://hdl.handle.net/11449/194551
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv Quimica Nova
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 466-471
dc.publisher.none.fl_str_mv Soc Brasileira Quimica
publisher.none.fl_str_mv Soc Brasileira Quimica
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1808128520335917056

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