Serological detection and risk factors for equine piroplasmosis in the semiarid region of Pernambuco, northeastern Brazil
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/s1984-29612019088 http://hdl.handle.net/11449/201373 |
Resumo: | Equine piroplasmosis, an economically important disease in horses, has so far not been reported in Pernambuco state, Brazil. This study aimed to evaluate the seroprevalence of anti-Babesia caballi and anti-Theileria equi antibodies based on the detection of these agents in equine blood and in ticks on horses in the municipality of Petrolina, Pernambuco, northeastern Brazil. Blood samples were drawn from 393 horses and sera were examined by ELISA. The presence of tick infestations was evaluated, and 101 ticks were subjected to DNA amplification for the detection of Babesia spp. by polymerase chain reaction (PCR). No parasites were detected in the blood smears. Anti-B. caballi and anti-T. equi antibodies were found in 27.2% (107/393) and 34.8% (137/393) horses, respectively. Infestation by Dermacentor nitens was detected in 4.3% (17/393) of the horses. There was no DNA amplification of the agents in ticks. The risk factors for the presence of anti-T. equi antibodies (P < 0.05) were: Purebred (P < 0.001), animals older than 156 months (P = 0.014), and the presence of ticks (P = 0.001). No risk factors for B. caballi were identified. This study confirmed the circulation of agents of equine piroplasmosis in the municipality of Petrolina, state of Pernambuco, Brazil. |
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Serological detection and risk factors for equine piroplasmosis in the semiarid region of Pernambuco, northeastern BrazilDetecção sorológica e fatores de riscos para piroplasmose equina na região semiárida de Pernambuco, Nordeste do BrasilBabesia caballiDiagnosticELISAHorsePCRTheileria equiEquine piroplasmosis, an economically important disease in horses, has so far not been reported in Pernambuco state, Brazil. This study aimed to evaluate the seroprevalence of anti-Babesia caballi and anti-Theileria equi antibodies based on the detection of these agents in equine blood and in ticks on horses in the municipality of Petrolina, Pernambuco, northeastern Brazil. Blood samples were drawn from 393 horses and sera were examined by ELISA. The presence of tick infestations was evaluated, and 101 ticks were subjected to DNA amplification for the detection of Babesia spp. by polymerase chain reaction (PCR). No parasites were detected in the blood smears. Anti-B. caballi and anti-T. equi antibodies were found in 27.2% (107/393) and 34.8% (137/393) horses, respectively. Infestation by Dermacentor nitens was detected in 4.3% (17/393) of the horses. There was no DNA amplification of the agents in ticks. The risk factors for the presence of anti-T. equi antibodies (P < 0.05) were: Purebred (P < 0.001), animals older than 156 months (P = 0.014), and the presence of ticks (P = 0.001). No risk factors for B. caballi were identified. This study confirmed the circulation of agents of equine piroplasmosis in the municipality of Petrolina, state of Pernambuco, Brazil.Laboratório de Doenças Parasitárias Universidade Federal do Vale do São Francisco – UNIVASFUniversidade Federal de Minas Gerais – UFMGFaculdade de Ciências Agrárias e Veterinárias – FCAV Universidade Estadual Paulista Júlio de Mesquita Filho – UNESPUnidade Acadêmica de Medicina Veterinária Universidade Federal de Campina Grande – UFCGFaculdade de Ciências Agrárias e Veterinárias – FCAV Universidade Estadual Paulista Júlio de Mesquita Filho – UNESPUniversidade Federal do Vale do São Francisco – UNIVASFUniversidade Federal de Minas Gerais (UFMG)Universidade Estadual Paulista (Unesp)Universidade Federal de Campina Grande – UFCGde Souza, Eline Almeida Rodriguesde Carvalho Araujo, AndreinaPires, Larissa Célly Souza RegisFreschi, Carla Roberta [UNESP]Azevedo, Sergio SantosMachado, Rosangela Zacarias [UNESP]Horta, Maurício Claudio2020-12-12T02:30:49Z2020-12-12T02:30:49Z2019-10-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article685-691application/pdfhttp://dx.doi.org/10.1590/s1984-29612019088Revista Brasileira de Parasitologia Veterinaria, v. 28, n. 4, p. 685-691, 2019.0103-846Xhttp://hdl.handle.net/11449/20137310.1590/s1984-29612019088S1984-296120190004006852-s2.0-85075958755S1984-29612019000400685.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengRevista Brasileira de Parasitologia Veterinariainfo:eu-repo/semantics/openAccess2024-06-07T13:01:43Zoai:repositorio.unesp.br:11449/201373Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T15:01:10.511234Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Serological detection and risk factors for equine piroplasmosis in the semiarid region of Pernambuco, northeastern Brazil Detecção sorológica e fatores de riscos para piroplasmose equina na região semiárida de Pernambuco, Nordeste do Brasil |
title |
Serological detection and risk factors for equine piroplasmosis in the semiarid region of Pernambuco, northeastern Brazil |
spellingShingle |
Serological detection and risk factors for equine piroplasmosis in the semiarid region of Pernambuco, northeastern Brazil de Souza, Eline Almeida Rodrigues Babesia caballi Diagnostic ELISA Horse PCR Theileria equi |
title_short |
Serological detection and risk factors for equine piroplasmosis in the semiarid region of Pernambuco, northeastern Brazil |
title_full |
Serological detection and risk factors for equine piroplasmosis in the semiarid region of Pernambuco, northeastern Brazil |
title_fullStr |
Serological detection and risk factors for equine piroplasmosis in the semiarid region of Pernambuco, northeastern Brazil |
title_full_unstemmed |
Serological detection and risk factors for equine piroplasmosis in the semiarid region of Pernambuco, northeastern Brazil |
title_sort |
Serological detection and risk factors for equine piroplasmosis in the semiarid region of Pernambuco, northeastern Brazil |
author |
de Souza, Eline Almeida Rodrigues |
author_facet |
de Souza, Eline Almeida Rodrigues de Carvalho Araujo, Andreina Pires, Larissa Célly Souza Regis Freschi, Carla Roberta [UNESP] Azevedo, Sergio Santos Machado, Rosangela Zacarias [UNESP] Horta, Maurício Claudio |
author_role |
author |
author2 |
de Carvalho Araujo, Andreina Pires, Larissa Célly Souza Regis Freschi, Carla Roberta [UNESP] Azevedo, Sergio Santos Machado, Rosangela Zacarias [UNESP] Horta, Maurício Claudio |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal do Vale do São Francisco – UNIVASF Universidade Federal de Minas Gerais (UFMG) Universidade Estadual Paulista (Unesp) Universidade Federal de Campina Grande – UFCG |
dc.contributor.author.fl_str_mv |
de Souza, Eline Almeida Rodrigues de Carvalho Araujo, Andreina Pires, Larissa Célly Souza Regis Freschi, Carla Roberta [UNESP] Azevedo, Sergio Santos Machado, Rosangela Zacarias [UNESP] Horta, Maurício Claudio |
dc.subject.por.fl_str_mv |
Babesia caballi Diagnostic ELISA Horse PCR Theileria equi |
topic |
Babesia caballi Diagnostic ELISA Horse PCR Theileria equi |
description |
Equine piroplasmosis, an economically important disease in horses, has so far not been reported in Pernambuco state, Brazil. This study aimed to evaluate the seroprevalence of anti-Babesia caballi and anti-Theileria equi antibodies based on the detection of these agents in equine blood and in ticks on horses in the municipality of Petrolina, Pernambuco, northeastern Brazil. Blood samples were drawn from 393 horses and sera were examined by ELISA. The presence of tick infestations was evaluated, and 101 ticks were subjected to DNA amplification for the detection of Babesia spp. by polymerase chain reaction (PCR). No parasites were detected in the blood smears. Anti-B. caballi and anti-T. equi antibodies were found in 27.2% (107/393) and 34.8% (137/393) horses, respectively. Infestation by Dermacentor nitens was detected in 4.3% (17/393) of the horses. There was no DNA amplification of the agents in ticks. The risk factors for the presence of anti-T. equi antibodies (P < 0.05) were: Purebred (P < 0.001), animals older than 156 months (P = 0.014), and the presence of ticks (P = 0.001). No risk factors for B. caballi were identified. This study confirmed the circulation of agents of equine piroplasmosis in the municipality of Petrolina, state of Pernambuco, Brazil. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-10-01 2020-12-12T02:30:49Z 2020-12-12T02:30:49Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/s1984-29612019088 Revista Brasileira de Parasitologia Veterinaria, v. 28, n. 4, p. 685-691, 2019. 0103-846X http://hdl.handle.net/11449/201373 10.1590/s1984-29612019088 S1984-29612019000400685 2-s2.0-85075958755 S1984-29612019000400685.pdf |
url |
http://dx.doi.org/10.1590/s1984-29612019088 http://hdl.handle.net/11449/201373 |
identifier_str_mv |
Revista Brasileira de Parasitologia Veterinaria, v. 28, n. 4, p. 685-691, 2019. 0103-846X 10.1590/s1984-29612019088 S1984-29612019000400685 2-s2.0-85075958755 S1984-29612019000400685.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Revista Brasileira de Parasitologia Veterinaria |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
685-691 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128446695473152 |