Diffusion analysis of one photosensitizer in bovine teeth using fluorescence optical imaging
Autor(a) principal: | |
---|---|
Data de Publicação: | 2012 |
Outros Autores: | , , , |
Tipo de documento: | Artigo de conferência |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1117/12.909136 http://hdl.handle.net/11449/16743 |
Resumo: | Some photosensitizers (PSs) used for PACT (Antimicrobial Photodynamic Therapy) show an affinity for bacterial walls and can be photo-activated to cause the desired damage. However, on dentine bacterias may be less susceptible to PACT as a result of limited penetration of the PS. The aim of this study was to evaluate the diffusion of one PS based on hematoporphyrin on dentine structures. Twelve bovine incisors were used. Class III cavities (3 x 3 x 1 mm) were prepared on the mesial or distal surfaces using a diamond bur. Photogem (R) solution at 1 mg/mL (10 uL for each cavity) was used. The experimental Groups were divided according to thickness of dentine remaining and etched or no-etched before the PS application. The fluorescence excitation source was a VelScope (R) system. For image capture a scientific CCD color camera PixelFly (R) was coupled to VelScope. For image acquisition and processing, a computational routine was developed at Matlab (R). Fick's Law was used to obtain the average diffusion coefficient of PS. Differences were found between all Groups. The longitudinal temporal diffusion was influenced by the different times, thickness and acid etching. |
id |
UNSP_e8903a09f12d0f41c2be20eadcff1b5e |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/16743 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Diffusion analysis of one photosensitizer in bovine teeth using fluorescence optical imagingFluorescence ImagePhotosensitizerPhotogemAntimicrobial Photodynamic TherapyDentineDiffusion CoefficientFick's LawSome photosensitizers (PSs) used for PACT (Antimicrobial Photodynamic Therapy) show an affinity for bacterial walls and can be photo-activated to cause the desired damage. However, on dentine bacterias may be less susceptible to PACT as a result of limited penetration of the PS. The aim of this study was to evaluate the diffusion of one PS based on hematoporphyrin on dentine structures. Twelve bovine incisors were used. Class III cavities (3 x 3 x 1 mm) were prepared on the mesial or distal surfaces using a diamond bur. Photogem (R) solution at 1 mg/mL (10 uL for each cavity) was used. The experimental Groups were divided according to thickness of dentine remaining and etched or no-etched before the PS application. The fluorescence excitation source was a VelScope (R) system. For image capture a scientific CCD color camera PixelFly (R) was coupled to VelScope. For image acquisition and processing, a computational routine was developed at Matlab (R). Fick's Law was used to obtain the average diffusion coefficient of PS. Differences were found between all Groups. The longitudinal temporal diffusion was influenced by the different times, thickness and acid etching.Univ Estadual Paulista UNESP, Araraquara Sch Dent, Dept Restorat Dent, BR-14801903 Araraquara, SP, BrazilUniv Estadual Paulista UNESP, Araraquara Sch Dent, Dept Restorat Dent, BR-14801903 Araraquara, SP, BrazilSpie - Int Soc Optical EngineeringUniversidade Estadual Paulista (Unesp)Montanha, S. [UNESP]Pratavieira, S.Jacomassi, D. P.Rastelli, Alessandra Nara de Souza [UNESP]Bagnato, V. S.2014-05-20T13:47:09Z2014-05-20T13:47:09Z2012-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObject7http://dx.doi.org/10.1117/12.909136Lasers In Dentistry Xviii. Bellingham: Spie-int Soc Optical Engineering, v. 8208, p. 7, 2012.0277-786Xhttp://hdl.handle.net/11449/1674310.1117/12.909136WOS:0003025981000176914969526213393Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengLasers In Dentistry Xviiiinfo:eu-repo/semantics/openAccess2021-10-22T13:22:22Zoai:repositorio.unesp.br:11449/16743Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T22:46:39.518656Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Diffusion analysis of one photosensitizer in bovine teeth using fluorescence optical imaging |
title |
Diffusion analysis of one photosensitizer in bovine teeth using fluorescence optical imaging |
spellingShingle |
Diffusion analysis of one photosensitizer in bovine teeth using fluorescence optical imaging Montanha, S. [UNESP] Fluorescence Image Photosensitizer Photogem Antimicrobial Photodynamic Therapy Dentine Diffusion Coefficient Fick's Law |
title_short |
Diffusion analysis of one photosensitizer in bovine teeth using fluorescence optical imaging |
title_full |
Diffusion analysis of one photosensitizer in bovine teeth using fluorescence optical imaging |
title_fullStr |
Diffusion analysis of one photosensitizer in bovine teeth using fluorescence optical imaging |
title_full_unstemmed |
Diffusion analysis of one photosensitizer in bovine teeth using fluorescence optical imaging |
title_sort |
Diffusion analysis of one photosensitizer in bovine teeth using fluorescence optical imaging |
author |
Montanha, S. [UNESP] |
author_facet |
Montanha, S. [UNESP] Pratavieira, S. Jacomassi, D. P. Rastelli, Alessandra Nara de Souza [UNESP] Bagnato, V. S. |
author_role |
author |
author2 |
Pratavieira, S. Jacomassi, D. P. Rastelli, Alessandra Nara de Souza [UNESP] Bagnato, V. S. |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Montanha, S. [UNESP] Pratavieira, S. Jacomassi, D. P. Rastelli, Alessandra Nara de Souza [UNESP] Bagnato, V. S. |
dc.subject.por.fl_str_mv |
Fluorescence Image Photosensitizer Photogem Antimicrobial Photodynamic Therapy Dentine Diffusion Coefficient Fick's Law |
topic |
Fluorescence Image Photosensitizer Photogem Antimicrobial Photodynamic Therapy Dentine Diffusion Coefficient Fick's Law |
description |
Some photosensitizers (PSs) used for PACT (Antimicrobial Photodynamic Therapy) show an affinity for bacterial walls and can be photo-activated to cause the desired damage. However, on dentine bacterias may be less susceptible to PACT as a result of limited penetration of the PS. The aim of this study was to evaluate the diffusion of one PS based on hematoporphyrin on dentine structures. Twelve bovine incisors were used. Class III cavities (3 x 3 x 1 mm) were prepared on the mesial or distal surfaces using a diamond bur. Photogem (R) solution at 1 mg/mL (10 uL for each cavity) was used. The experimental Groups were divided according to thickness of dentine remaining and etched or no-etched before the PS application. The fluorescence excitation source was a VelScope (R) system. For image capture a scientific CCD color camera PixelFly (R) was coupled to VelScope. For image acquisition and processing, a computational routine was developed at Matlab (R). Fick's Law was used to obtain the average diffusion coefficient of PS. Differences were found between all Groups. The longitudinal temporal diffusion was influenced by the different times, thickness and acid etching. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-01-01 2014-05-20T13:47:09Z 2014-05-20T13:47:09Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/conferenceObject |
format |
conferenceObject |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1117/12.909136 Lasers In Dentistry Xviii. Bellingham: Spie-int Soc Optical Engineering, v. 8208, p. 7, 2012. 0277-786X http://hdl.handle.net/11449/16743 10.1117/12.909136 WOS:000302598100017 6914969526213393 |
url |
http://dx.doi.org/10.1117/12.909136 http://hdl.handle.net/11449/16743 |
identifier_str_mv |
Lasers In Dentistry Xviii. Bellingham: Spie-int Soc Optical Engineering, v. 8208, p. 7, 2012. 0277-786X 10.1117/12.909136 WOS:000302598100017 6914969526213393 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Lasers In Dentistry Xviii |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
7 |
dc.publisher.none.fl_str_mv |
Spie - Int Soc Optical Engineering |
publisher.none.fl_str_mv |
Spie - Int Soc Optical Engineering |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129461577580544 |