Prostanoids counterbalance the synergism between endothelin-1 and angiotensin II in mesenteric veins of trained rats

Detalhes bibliográficos
Autor(a) principal: Chies, Agnaldo Bruno
Data de Publicação: 2017
Outros Autores: de Oliveira, Priscilla Bianca, Rossignoli, Patrícia de Souza [UNESP], Baptista, Rafaela de Fátima Ferreira, de Lábio, Roger William, Payão, Spencer Luiz Marques
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.peptides.2016.12.013
http://hdl.handle.net/11449/173973
Resumo: Exercise-induced adaptations of the modulating mechanisms that influence the angiotensin (Ang II) responses assume different features depending on the venous bed. In femoral veins, exercise mobilizes vasodilator prostanoids to cooperate with NO in order to maintain reduced Ang II responses. On the other hand, exercise's influence on the Ang II responses in veins that drain blood from the mesenteric region has been poorly described. Therefore, the present study aimed to identify the effects of a single bout of exercise, as well as exercise training, on the Ang II responses in mesenteric veins. The present study also aimed to investigate the involvement of prostanoids, NO and ET-1 in eventual exercise-induced modifications in these veins. To this end, mesenteric veins taken from resting-sedentary, exercised-sedentary, resting-trained and exercised-trained animals were studied in organ baths. In addition, the mRNA expression of prepro-endothelin-1 (ppET-1), as well as that of the ETA and ETB receptors, were quantified by real-time PCR in these veins. The results show that, either in absence or in presence of L-NAME, the Ang II responses were not different between groups. In the presence of indomethacin, higher Ang II responses were observed in the resting-trained animals than in the resting-sedentary animals. This difference, however, disappeared when L-NAME, BQ-123 or BQ-788 were added during incubation. In addition, no differences in ppET-1, ETA or ETB mRNA expression were observed between groups. Furthermore, in the presence of PD123,319, the Ang II responses in the exercised-sedentary animals were higher than those in the resting-sedentary animals. In conclusion, exercise training mobilizes endothelin-1 (ET-1) to reinforce the Ang II-induced responses mainly through ETA activation. On the other hand, vasodilator prostanoids are mobilized to act in parallel with NO in order to counterbalance the Ang II responses that have been potentiated by ET-1 in these trained animals.
id UNSP_e891854b439b7d864c2aee5f50d0bbfb
oai_identifier_str oai:repositorio.unesp.br:11449/173973
network_acronym_str UNSP
network_name_str Repositório Institucional da UNESP
repository_id_str 2946
spelling Prostanoids counterbalance the synergism between endothelin-1 and angiotensin II in mesenteric veins of trained ratsAngiotensin IIEndothelin-1ExerciseNitric oxideProstanoidsVeinExercise-induced adaptations of the modulating mechanisms that influence the angiotensin (Ang II) responses assume different features depending on the venous bed. In femoral veins, exercise mobilizes vasodilator prostanoids to cooperate with NO in order to maintain reduced Ang II responses. On the other hand, exercise's influence on the Ang II responses in veins that drain blood from the mesenteric region has been poorly described. Therefore, the present study aimed to identify the effects of a single bout of exercise, as well as exercise training, on the Ang II responses in mesenteric veins. The present study also aimed to investigate the involvement of prostanoids, NO and ET-1 in eventual exercise-induced modifications in these veins. To this end, mesenteric veins taken from resting-sedentary, exercised-sedentary, resting-trained and exercised-trained animals were studied in organ baths. In addition, the mRNA expression of prepro-endothelin-1 (ppET-1), as well as that of the ETA and ETB receptors, were quantified by real-time PCR in these veins. The results show that, either in absence or in presence of L-NAME, the Ang II responses were not different between groups. In the presence of indomethacin, higher Ang II responses were observed in the resting-trained animals than in the resting-sedentary animals. This difference, however, disappeared when L-NAME, BQ-123 or BQ-788 were added during incubation. In addition, no differences in ppET-1, ETA or ETB mRNA expression were observed between groups. Furthermore, in the presence of PD123,319, the Ang II responses in the exercised-sedentary animals were higher than those in the resting-sedentary animals. In conclusion, exercise training mobilizes endothelin-1 (ET-1) to reinforce the Ang II-induced responses mainly through ETA activation. On the other hand, vasodilator prostanoids are mobilized to act in parallel with NO in order to counterbalance the Ang II responses that have been potentiated by ET-1 in these trained animals.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Laboratory of Pharmacology Marília Medical SchoolDepartment of Physiotherapy and Occupational Therapy São Paulo State University (UNESP), MaríliaFaculty Esefap of TupãFaculty of Alta Paulista (FAP), TupãLaboratory of Genetic Marília Medical School, São PauloDepartment of Physiotherapy and Occupational Therapy São Paulo State University (UNESP), MaríliaMarília Medical SchoolUniversidade Estadual Paulista (Unesp)Faculty Esefap of TupãFaculty of Alta Paulista (FAP)Chies, Agnaldo Brunode Oliveira, Priscilla BiancaRossignoli, Patrícia de Souza [UNESP]Baptista, Rafaela de Fátima Ferreirade Lábio, Roger WilliamPayão, Spencer Luiz Marques2018-12-11T17:08:35Z2018-12-11T17:08:35Z2017-02-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article67-73application/pdfhttp://dx.doi.org/10.1016/j.peptides.2016.12.013Peptides, v. 88, p. 67-73.1873-51690196-9781http://hdl.handle.net/11449/17397310.1016/j.peptides.2016.12.0132-s2.0-850070306752-s2.0-85007030675.pdf26229754535630850000-0001-6946-1145Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPeptides1,001info:eu-repo/semantics/openAccess2024-08-09T15:17:10Zoai:repositorio.unesp.br:11449/173973Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-09T15:17:10Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Prostanoids counterbalance the synergism between endothelin-1 and angiotensin II in mesenteric veins of trained rats
title Prostanoids counterbalance the synergism between endothelin-1 and angiotensin II in mesenteric veins of trained rats
spellingShingle Prostanoids counterbalance the synergism between endothelin-1 and angiotensin II in mesenteric veins of trained rats
Chies, Agnaldo Bruno
Angiotensin II
Endothelin-1
Exercise
Nitric oxide
Prostanoids
Vein
title_short Prostanoids counterbalance the synergism between endothelin-1 and angiotensin II in mesenteric veins of trained rats
title_full Prostanoids counterbalance the synergism between endothelin-1 and angiotensin II in mesenteric veins of trained rats
title_fullStr Prostanoids counterbalance the synergism between endothelin-1 and angiotensin II in mesenteric veins of trained rats
title_full_unstemmed Prostanoids counterbalance the synergism between endothelin-1 and angiotensin II in mesenteric veins of trained rats
title_sort Prostanoids counterbalance the synergism between endothelin-1 and angiotensin II in mesenteric veins of trained rats
author Chies, Agnaldo Bruno
author_facet Chies, Agnaldo Bruno
de Oliveira, Priscilla Bianca
Rossignoli, Patrícia de Souza [UNESP]
Baptista, Rafaela de Fátima Ferreira
de Lábio, Roger William
Payão, Spencer Luiz Marques
author_role author
author2 de Oliveira, Priscilla Bianca
Rossignoli, Patrícia de Souza [UNESP]
Baptista, Rafaela de Fátima Ferreira
de Lábio, Roger William
Payão, Spencer Luiz Marques
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Marília Medical School
Universidade Estadual Paulista (Unesp)
Faculty Esefap of Tupã
Faculty of Alta Paulista (FAP)
dc.contributor.author.fl_str_mv Chies, Agnaldo Bruno
de Oliveira, Priscilla Bianca
Rossignoli, Patrícia de Souza [UNESP]
Baptista, Rafaela de Fátima Ferreira
de Lábio, Roger William
Payão, Spencer Luiz Marques
dc.subject.por.fl_str_mv Angiotensin II
Endothelin-1
Exercise
Nitric oxide
Prostanoids
Vein
topic Angiotensin II
Endothelin-1
Exercise
Nitric oxide
Prostanoids
Vein
description Exercise-induced adaptations of the modulating mechanisms that influence the angiotensin (Ang II) responses assume different features depending on the venous bed. In femoral veins, exercise mobilizes vasodilator prostanoids to cooperate with NO in order to maintain reduced Ang II responses. On the other hand, exercise's influence on the Ang II responses in veins that drain blood from the mesenteric region has been poorly described. Therefore, the present study aimed to identify the effects of a single bout of exercise, as well as exercise training, on the Ang II responses in mesenteric veins. The present study also aimed to investigate the involvement of prostanoids, NO and ET-1 in eventual exercise-induced modifications in these veins. To this end, mesenteric veins taken from resting-sedentary, exercised-sedentary, resting-trained and exercised-trained animals were studied in organ baths. In addition, the mRNA expression of prepro-endothelin-1 (ppET-1), as well as that of the ETA and ETB receptors, were quantified by real-time PCR in these veins. The results show that, either in absence or in presence of L-NAME, the Ang II responses were not different between groups. In the presence of indomethacin, higher Ang II responses were observed in the resting-trained animals than in the resting-sedentary animals. This difference, however, disappeared when L-NAME, BQ-123 or BQ-788 were added during incubation. In addition, no differences in ppET-1, ETA or ETB mRNA expression were observed between groups. Furthermore, in the presence of PD123,319, the Ang II responses in the exercised-sedentary animals were higher than those in the resting-sedentary animals. In conclusion, exercise training mobilizes endothelin-1 (ET-1) to reinforce the Ang II-induced responses mainly through ETA activation. On the other hand, vasodilator prostanoids are mobilized to act in parallel with NO in order to counterbalance the Ang II responses that have been potentiated by ET-1 in these trained animals.
publishDate 2017
dc.date.none.fl_str_mv 2017-02-01
2018-12-11T17:08:35Z
2018-12-11T17:08:35Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.peptides.2016.12.013
Peptides, v. 88, p. 67-73.
1873-5169
0196-9781
http://hdl.handle.net/11449/173973
10.1016/j.peptides.2016.12.013
2-s2.0-85007030675
2-s2.0-85007030675.pdf
2622975453563085
0000-0001-6946-1145
url http://dx.doi.org/10.1016/j.peptides.2016.12.013
http://hdl.handle.net/11449/173973
identifier_str_mv Peptides, v. 88, p. 67-73.
1873-5169
0196-9781
10.1016/j.peptides.2016.12.013
2-s2.0-85007030675
2-s2.0-85007030675.pdf
2622975453563085
0000-0001-6946-1145
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Peptides
1,001
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 67-73
application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1808128127999672320