Prostanoids counterbalance the synergism between endothelin-1 and angiotensin II in mesenteric veins of trained rats
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.peptides.2016.12.013 http://hdl.handle.net/11449/173973 |
Resumo: | Exercise-induced adaptations of the modulating mechanisms that influence the angiotensin (Ang II) responses assume different features depending on the venous bed. In femoral veins, exercise mobilizes vasodilator prostanoids to cooperate with NO in order to maintain reduced Ang II responses. On the other hand, exercise's influence on the Ang II responses in veins that drain blood from the mesenteric region has been poorly described. Therefore, the present study aimed to identify the effects of a single bout of exercise, as well as exercise training, on the Ang II responses in mesenteric veins. The present study also aimed to investigate the involvement of prostanoids, NO and ET-1 in eventual exercise-induced modifications in these veins. To this end, mesenteric veins taken from resting-sedentary, exercised-sedentary, resting-trained and exercised-trained animals were studied in organ baths. In addition, the mRNA expression of prepro-endothelin-1 (ppET-1), as well as that of the ETA and ETB receptors, were quantified by real-time PCR in these veins. The results show that, either in absence or in presence of L-NAME, the Ang II responses were not different between groups. In the presence of indomethacin, higher Ang II responses were observed in the resting-trained animals than in the resting-sedentary animals. This difference, however, disappeared when L-NAME, BQ-123 or BQ-788 were added during incubation. In addition, no differences in ppET-1, ETA or ETB mRNA expression were observed between groups. Furthermore, in the presence of PD123,319, the Ang II responses in the exercised-sedentary animals were higher than those in the resting-sedentary animals. In conclusion, exercise training mobilizes endothelin-1 (ET-1) to reinforce the Ang II-induced responses mainly through ETA activation. On the other hand, vasodilator prostanoids are mobilized to act in parallel with NO in order to counterbalance the Ang II responses that have been potentiated by ET-1 in these trained animals. |
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Prostanoids counterbalance the synergism between endothelin-1 and angiotensin II in mesenteric veins of trained ratsAngiotensin IIEndothelin-1ExerciseNitric oxideProstanoidsVeinExercise-induced adaptations of the modulating mechanisms that influence the angiotensin (Ang II) responses assume different features depending on the venous bed. In femoral veins, exercise mobilizes vasodilator prostanoids to cooperate with NO in order to maintain reduced Ang II responses. On the other hand, exercise's influence on the Ang II responses in veins that drain blood from the mesenteric region has been poorly described. Therefore, the present study aimed to identify the effects of a single bout of exercise, as well as exercise training, on the Ang II responses in mesenteric veins. The present study also aimed to investigate the involvement of prostanoids, NO and ET-1 in eventual exercise-induced modifications in these veins. To this end, mesenteric veins taken from resting-sedentary, exercised-sedentary, resting-trained and exercised-trained animals were studied in organ baths. In addition, the mRNA expression of prepro-endothelin-1 (ppET-1), as well as that of the ETA and ETB receptors, were quantified by real-time PCR in these veins. The results show that, either in absence or in presence of L-NAME, the Ang II responses were not different between groups. In the presence of indomethacin, higher Ang II responses were observed in the resting-trained animals than in the resting-sedentary animals. This difference, however, disappeared when L-NAME, BQ-123 or BQ-788 were added during incubation. In addition, no differences in ppET-1, ETA or ETB mRNA expression were observed between groups. Furthermore, in the presence of PD123,319, the Ang II responses in the exercised-sedentary animals were higher than those in the resting-sedentary animals. In conclusion, exercise training mobilizes endothelin-1 (ET-1) to reinforce the Ang II-induced responses mainly through ETA activation. On the other hand, vasodilator prostanoids are mobilized to act in parallel with NO in order to counterbalance the Ang II responses that have been potentiated by ET-1 in these trained animals.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Laboratory of Pharmacology Marília Medical SchoolDepartment of Physiotherapy and Occupational Therapy São Paulo State University (UNESP), MaríliaFaculty Esefap of TupãFaculty of Alta Paulista (FAP), TupãLaboratory of Genetic Marília Medical School, São PauloDepartment of Physiotherapy and Occupational Therapy São Paulo State University (UNESP), MaríliaMarília Medical SchoolUniversidade Estadual Paulista (Unesp)Faculty Esefap of TupãFaculty of Alta Paulista (FAP)Chies, Agnaldo Brunode Oliveira, Priscilla BiancaRossignoli, Patrícia de Souza [UNESP]Baptista, Rafaela de Fátima Ferreirade Lábio, Roger WilliamPayão, Spencer Luiz Marques2018-12-11T17:08:35Z2018-12-11T17:08:35Z2017-02-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article67-73application/pdfhttp://dx.doi.org/10.1016/j.peptides.2016.12.013Peptides, v. 88, p. 67-73.1873-51690196-9781http://hdl.handle.net/11449/17397310.1016/j.peptides.2016.12.0132-s2.0-850070306752-s2.0-85007030675.pdf26229754535630850000-0001-6946-1145Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPeptides1,001info:eu-repo/semantics/openAccess2024-08-09T15:17:10Zoai:repositorio.unesp.br:11449/173973Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-09T15:17:10Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Prostanoids counterbalance the synergism between endothelin-1 and angiotensin II in mesenteric veins of trained rats |
title |
Prostanoids counterbalance the synergism between endothelin-1 and angiotensin II in mesenteric veins of trained rats |
spellingShingle |
Prostanoids counterbalance the synergism between endothelin-1 and angiotensin II in mesenteric veins of trained rats Chies, Agnaldo Bruno Angiotensin II Endothelin-1 Exercise Nitric oxide Prostanoids Vein |
title_short |
Prostanoids counterbalance the synergism between endothelin-1 and angiotensin II in mesenteric veins of trained rats |
title_full |
Prostanoids counterbalance the synergism between endothelin-1 and angiotensin II in mesenteric veins of trained rats |
title_fullStr |
Prostanoids counterbalance the synergism between endothelin-1 and angiotensin II in mesenteric veins of trained rats |
title_full_unstemmed |
Prostanoids counterbalance the synergism between endothelin-1 and angiotensin II in mesenteric veins of trained rats |
title_sort |
Prostanoids counterbalance the synergism between endothelin-1 and angiotensin II in mesenteric veins of trained rats |
author |
Chies, Agnaldo Bruno |
author_facet |
Chies, Agnaldo Bruno de Oliveira, Priscilla Bianca Rossignoli, Patrícia de Souza [UNESP] Baptista, Rafaela de Fátima Ferreira de Lábio, Roger William Payão, Spencer Luiz Marques |
author_role |
author |
author2 |
de Oliveira, Priscilla Bianca Rossignoli, Patrícia de Souza [UNESP] Baptista, Rafaela de Fátima Ferreira de Lábio, Roger William Payão, Spencer Luiz Marques |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Marília Medical School Universidade Estadual Paulista (Unesp) Faculty Esefap of Tupã Faculty of Alta Paulista (FAP) |
dc.contributor.author.fl_str_mv |
Chies, Agnaldo Bruno de Oliveira, Priscilla Bianca Rossignoli, Patrícia de Souza [UNESP] Baptista, Rafaela de Fátima Ferreira de Lábio, Roger William Payão, Spencer Luiz Marques |
dc.subject.por.fl_str_mv |
Angiotensin II Endothelin-1 Exercise Nitric oxide Prostanoids Vein |
topic |
Angiotensin II Endothelin-1 Exercise Nitric oxide Prostanoids Vein |
description |
Exercise-induced adaptations of the modulating mechanisms that influence the angiotensin (Ang II) responses assume different features depending on the venous bed. In femoral veins, exercise mobilizes vasodilator prostanoids to cooperate with NO in order to maintain reduced Ang II responses. On the other hand, exercise's influence on the Ang II responses in veins that drain blood from the mesenteric region has been poorly described. Therefore, the present study aimed to identify the effects of a single bout of exercise, as well as exercise training, on the Ang II responses in mesenteric veins. The present study also aimed to investigate the involvement of prostanoids, NO and ET-1 in eventual exercise-induced modifications in these veins. To this end, mesenteric veins taken from resting-sedentary, exercised-sedentary, resting-trained and exercised-trained animals were studied in organ baths. In addition, the mRNA expression of prepro-endothelin-1 (ppET-1), as well as that of the ETA and ETB receptors, were quantified by real-time PCR in these veins. The results show that, either in absence or in presence of L-NAME, the Ang II responses were not different between groups. In the presence of indomethacin, higher Ang II responses were observed in the resting-trained animals than in the resting-sedentary animals. This difference, however, disappeared when L-NAME, BQ-123 or BQ-788 were added during incubation. In addition, no differences in ppET-1, ETA or ETB mRNA expression were observed between groups. Furthermore, in the presence of PD123,319, the Ang II responses in the exercised-sedentary animals were higher than those in the resting-sedentary animals. In conclusion, exercise training mobilizes endothelin-1 (ET-1) to reinforce the Ang II-induced responses mainly through ETA activation. On the other hand, vasodilator prostanoids are mobilized to act in parallel with NO in order to counterbalance the Ang II responses that have been potentiated by ET-1 in these trained animals. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-02-01 2018-12-11T17:08:35Z 2018-12-11T17:08:35Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.peptides.2016.12.013 Peptides, v. 88, p. 67-73. 1873-5169 0196-9781 http://hdl.handle.net/11449/173973 10.1016/j.peptides.2016.12.013 2-s2.0-85007030675 2-s2.0-85007030675.pdf 2622975453563085 0000-0001-6946-1145 |
url |
http://dx.doi.org/10.1016/j.peptides.2016.12.013 http://hdl.handle.net/11449/173973 |
identifier_str_mv |
Peptides, v. 88, p. 67-73. 1873-5169 0196-9781 10.1016/j.peptides.2016.12.013 2-s2.0-85007030675 2-s2.0-85007030675.pdf 2622975453563085 0000-0001-6946-1145 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Peptides 1,001 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
67-73 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128127999672320 |