Transcriptional Control of the Production of Aspergillus fumigatus Conidia-Borne Secondary Metabolite Fumiquinazoline C Important for Phagocytosis Protection
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1093/genetics/iyab036 http://hdl.handle.net/11449/210378 |
Resumo: | Aspergillus fumigatus produces diverse secondary metabolites whose biological functions and regulation remain to be understood. Despite the importance of the conidia for this fungus, the role of the conidia-born metabolite fumiquinazoline C (FqC) is unclear. Here, we describe a dual function of the cell-wall integrity pathway in regulating FqC biosynthesis dictated by the MAPK kinase MpkA, which phosphorylates one of the nonribosomal peptide synthetases enzymes of the cluster (FmqC), and the transcription factor RlmA, which directly regulates the expression of fmq genes. Another level of crosstalk between the FqC regulation and the cell physiology is described since the deletion of the stress-responsive transcription factor sebA provokes derepression of the fmq cluster and overproduction of FqC. Thus, we describe a mechanism by which A. fumigatus controls FqC biosynthesis orchestrated by MpkA-RlmA and SebA and hence enabling survival and adaptation to the environmental niche, given that FqC is a deterrent of ameba predation. |
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Transcriptional Control of the Production of Aspergillus fumigatus Conidia-Borne Secondary Metabolite Fumiquinazoline C Important for Phagocytosis Protectioncell wall integrity pathwayfumiquinazoline CpkcAmpkArlmAsebAamoeba predationAspergillus fumigatus produces diverse secondary metabolites whose biological functions and regulation remain to be understood. Despite the importance of the conidia for this fungus, the role of the conidia-born metabolite fumiquinazoline C (FqC) is unclear. Here, we describe a dual function of the cell-wall integrity pathway in regulating FqC biosynthesis dictated by the MAPK kinase MpkA, which phosphorylates one of the nonribosomal peptide synthetases enzymes of the cluster (FmqC), and the transcription factor RlmA, which directly regulates the expression of fmq genes. Another level of crosstalk between the FqC regulation and the cell physiology is described since the deletion of the stress-responsive transcription factor sebA provokes derepression of the fmq cluster and overproduction of FqC. Thus, we describe a mechanism by which A. fumigatus controls FqC biosynthesis orchestrated by MpkA-RlmA and SebA and hence enabling survival and adaptation to the environmental niche, given that FqC is a deterrent of ameba predation.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Univ Fed Sao Carlos, Ctr Ciencias Biol & Saude, Dept Genet & Evolucao, Sao Carlos, SP, BrazilUniv Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Dept Ciencias Farmaceut, Ribeirao Preto, BrazilUniv Fed ABC, Ctr Ciencias Nat & Humanas, Santo Andre, SP, BrazilUniv Estadual Paulista, Inst Quim Araraquara, Dept Bioquim & Tecnol, Araraquara, SP, BrazilLeibniz Inst Nat Prod Res & Infect Biol, Leibniz Res Grp Biobricks Microbial Nat Prod Synt, Hans Knoll Inst, Jena, GermanyUniv Estadual Campinas, Inst Quim, Campinas, SP, BrazilUniv Estadual Paulista, Inst Quim Araraquara, Dept Bioquim & Tecnol, Araraquara, SP, BrazilFAPESP: 2015/17541-0FAPESP: 2016/07870-9FAPESP: 2017/19694-3CNPq: 301058/2019-9CNPq: 404735/2018-5CNPq: 462383/2014-8Oxford Univ Press IncUniversidade Federal de São Carlos (UFSCar)Universidade de São Paulo (USP)Universidade Federal do ABC (UFABC)Universidade Estadual Paulista (Unesp)Leibniz Inst Nat Prod Res & Infect BiolUniversidade Estadual de Campinas (UNICAMP)Rocha, Marina CamposTadini Marilhano Fabri, Joao HenriqueSilva, Lilian PereiraFigueiredo Angolini, Celio FernandoBertolini, Maria Celia [UNESP]Cunha, Anderson Ferreira daValiante, VitoGoldman, Gustavo HenriqueFill, Taicia PachecoMalavazi, Iran2021-06-25T15:06:36Z2021-06-25T15:06:36Z2021-05-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article18http://dx.doi.org/10.1093/genetics/iyab036Genetics. Cary: Oxford Univ Press Inc, v. 218, n. 1, 18 p., 2021.0016-6731http://hdl.handle.net/11449/21037810.1093/genetics/iyab036WOS:000656158600010Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengGeneticsinfo:eu-repo/semantics/openAccess2021-10-23T20:17:27Zoai:repositorio.unesp.br:11449/210378Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T17:01:47.586999Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Transcriptional Control of the Production of Aspergillus fumigatus Conidia-Borne Secondary Metabolite Fumiquinazoline C Important for Phagocytosis Protection |
title |
Transcriptional Control of the Production of Aspergillus fumigatus Conidia-Borne Secondary Metabolite Fumiquinazoline C Important for Phagocytosis Protection |
spellingShingle |
Transcriptional Control of the Production of Aspergillus fumigatus Conidia-Borne Secondary Metabolite Fumiquinazoline C Important for Phagocytosis Protection Rocha, Marina Campos cell wall integrity pathway fumiquinazoline C pkcA mpkA rlmA sebA amoeba predation |
title_short |
Transcriptional Control of the Production of Aspergillus fumigatus Conidia-Borne Secondary Metabolite Fumiquinazoline C Important for Phagocytosis Protection |
title_full |
Transcriptional Control of the Production of Aspergillus fumigatus Conidia-Borne Secondary Metabolite Fumiquinazoline C Important for Phagocytosis Protection |
title_fullStr |
Transcriptional Control of the Production of Aspergillus fumigatus Conidia-Borne Secondary Metabolite Fumiquinazoline C Important for Phagocytosis Protection |
title_full_unstemmed |
Transcriptional Control of the Production of Aspergillus fumigatus Conidia-Borne Secondary Metabolite Fumiquinazoline C Important for Phagocytosis Protection |
title_sort |
Transcriptional Control of the Production of Aspergillus fumigatus Conidia-Borne Secondary Metabolite Fumiquinazoline C Important for Phagocytosis Protection |
author |
Rocha, Marina Campos |
author_facet |
Rocha, Marina Campos Tadini Marilhano Fabri, Joao Henrique Silva, Lilian Pereira Figueiredo Angolini, Celio Fernando Bertolini, Maria Celia [UNESP] Cunha, Anderson Ferreira da Valiante, Vito Goldman, Gustavo Henrique Fill, Taicia Pacheco Malavazi, Iran |
author_role |
author |
author2 |
Tadini Marilhano Fabri, Joao Henrique Silva, Lilian Pereira Figueiredo Angolini, Celio Fernando Bertolini, Maria Celia [UNESP] Cunha, Anderson Ferreira da Valiante, Vito Goldman, Gustavo Henrique Fill, Taicia Pacheco Malavazi, Iran |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal de São Carlos (UFSCar) Universidade de São Paulo (USP) Universidade Federal do ABC (UFABC) Universidade Estadual Paulista (Unesp) Leibniz Inst Nat Prod Res & Infect Biol Universidade Estadual de Campinas (UNICAMP) |
dc.contributor.author.fl_str_mv |
Rocha, Marina Campos Tadini Marilhano Fabri, Joao Henrique Silva, Lilian Pereira Figueiredo Angolini, Celio Fernando Bertolini, Maria Celia [UNESP] Cunha, Anderson Ferreira da Valiante, Vito Goldman, Gustavo Henrique Fill, Taicia Pacheco Malavazi, Iran |
dc.subject.por.fl_str_mv |
cell wall integrity pathway fumiquinazoline C pkcA mpkA rlmA sebA amoeba predation |
topic |
cell wall integrity pathway fumiquinazoline C pkcA mpkA rlmA sebA amoeba predation |
description |
Aspergillus fumigatus produces diverse secondary metabolites whose biological functions and regulation remain to be understood. Despite the importance of the conidia for this fungus, the role of the conidia-born metabolite fumiquinazoline C (FqC) is unclear. Here, we describe a dual function of the cell-wall integrity pathway in regulating FqC biosynthesis dictated by the MAPK kinase MpkA, which phosphorylates one of the nonribosomal peptide synthetases enzymes of the cluster (FmqC), and the transcription factor RlmA, which directly regulates the expression of fmq genes. Another level of crosstalk between the FqC regulation and the cell physiology is described since the deletion of the stress-responsive transcription factor sebA provokes derepression of the fmq cluster and overproduction of FqC. Thus, we describe a mechanism by which A. fumigatus controls FqC biosynthesis orchestrated by MpkA-RlmA and SebA and hence enabling survival and adaptation to the environmental niche, given that FqC is a deterrent of ameba predation. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-06-25T15:06:36Z 2021-06-25T15:06:36Z 2021-05-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1093/genetics/iyab036 Genetics. Cary: Oxford Univ Press Inc, v. 218, n. 1, 18 p., 2021. 0016-6731 http://hdl.handle.net/11449/210378 10.1093/genetics/iyab036 WOS:000656158600010 |
url |
http://dx.doi.org/10.1093/genetics/iyab036 http://hdl.handle.net/11449/210378 |
identifier_str_mv |
Genetics. Cary: Oxford Univ Press Inc, v. 218, n. 1, 18 p., 2021. 0016-6731 10.1093/genetics/iyab036 WOS:000656158600010 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Genetics |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
18 |
dc.publisher.none.fl_str_mv |
Oxford Univ Press Inc |
publisher.none.fl_str_mv |
Oxford Univ Press Inc |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128742663389184 |