Efficacy of melatonin, IL-25 and siIL-17B in tumorigenesis-associated properties of breast cancer cell lines
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.lfs.2017.06.013 http://hdl.handle.net/11449/169878 |
Resumo: | Mammary tumorigenesis can be modulated by melatonin, which has oncostatic action mediated by multiple mechanisms, including the inhibition of the activity of transcription factors such as NF-κB and modulation of interleukins (ILs) expression. IL-25 is an active cytokine that induces apoptosis in tumor cells due to differential expression of its receptor (IL-17RB). IL-17B competes with IL-25 for binding to IL-17RB in tumor cells, promoting tumorigenesis. This study purpose is to address the possibility of engaging IL-25/IL-17RB signaling to enhance the effect of melatonin on breast cancer cells. Breast cancer cell lines were cultured monolayers and 3D structures and treated with melatonin, IL-25, siIL-17B, each alone or in combination. Cell viability, gene and protein expression of caspase-3, cleaved caspase-3 and VEGF-A were performed by qPCR and immunofluorescence. In addition, an apoptosis membrane array was performed in metastatic cells. Treatments with melatonin and IL-25 significantly reduced tumor cells viability at 1 mM and 1 ng/mL, respectively, but did not alter cell viability of a non-tumorigenic epithelial cell line (MCF-10A). All treatments, alone and combined, significantly increased cleaved caspase-3 in tumor cells grown as monolayers and 3D structures (p < 0.05). Semi-quantitative analysis of apoptosis pathway proteins showed an increase of CYTO-C, DR6, IGFBP-3, IGFBP-5, IGFPB-6, IGF-1, IGF-1R, Livin, P21, P53, TNFRII, XIAP and hTRA proteins and reduction of caspase-3 (p < 0.05) after melatonin treatment. All treatments reduced VEGF-A protein expression in tumor cells (p < 0.05). Our results suggest therapeutic potential, with oncostatic effectiveness, pro-apoptotic and anti-angiogenic properties for melatonin and IL-25-driven signaling in breast cancer cells. |
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Efficacy of melatonin, IL-25 and siIL-17B in tumorigenesis-associated properties of breast cancer cell linesApoptosisBreast cancerInterleukin-17BInterleukin-17EInterleukin-25MelatoninVEGFMammary tumorigenesis can be modulated by melatonin, which has oncostatic action mediated by multiple mechanisms, including the inhibition of the activity of transcription factors such as NF-κB and modulation of interleukins (ILs) expression. IL-25 is an active cytokine that induces apoptosis in tumor cells due to differential expression of its receptor (IL-17RB). IL-17B competes with IL-25 for binding to IL-17RB in tumor cells, promoting tumorigenesis. This study purpose is to address the possibility of engaging IL-25/IL-17RB signaling to enhance the effect of melatonin on breast cancer cells. Breast cancer cell lines were cultured monolayers and 3D structures and treated with melatonin, IL-25, siIL-17B, each alone or in combination. Cell viability, gene and protein expression of caspase-3, cleaved caspase-3 and VEGF-A were performed by qPCR and immunofluorescence. In addition, an apoptosis membrane array was performed in metastatic cells. Treatments with melatonin and IL-25 significantly reduced tumor cells viability at 1 mM and 1 ng/mL, respectively, but did not alter cell viability of a non-tumorigenic epithelial cell line (MCF-10A). All treatments, alone and combined, significantly increased cleaved caspase-3 in tumor cells grown as monolayers and 3D structures (p < 0.05). Semi-quantitative analysis of apoptosis pathway proteins showed an increase of CYTO-C, DR6, IGFBP-3, IGFBP-5, IGFPB-6, IGF-1, IGF-1R, Livin, P21, P53, TNFRII, XIAP and hTRA proteins and reduction of caspase-3 (p < 0.05) after melatonin treatment. All treatments reduced VEGF-A protein expression in tumor cells (p < 0.05). Our results suggest therapeutic potential, with oncostatic effectiveness, pro-apoptotic and anti-angiogenic properties for melatonin and IL-25-driven signaling in breast cancer cells.Faculdade de Medicina de São José do Rio PretoFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Universidade Estadual Paulista “Júlio de Mesquita Filho” (UNESP/IBILCE) Programa de Pós-Graduação em Genética, São José do Rio PretoFaculdade de Medicina de São José do Rio Preto (FAMERP) Laboratório de Investigação Molecular do Câncer (LIMC), São José do Rio PretoTumor Imaging Angiogenesis Laboratory Georgia Cancer Center Augusta UniversityDepartment of Biomedical Sciences Ontario Veterinary College University of GuelphUniversidade Estadual Paulista “Júlio de Mesquita Filho” (UNESP/IBILCE) Programa de Pós-Graduação em Genética, São José do Rio PretoFAPESP: 2012/02128-1FAPESP: 2012/06098-0Universidade Estadual Paulista (Unesp)Laboratório de Investigação Molecular do Câncer (LIMC)Augusta UniversityUniversity of GuelphGelaleti, Gabriela Bottaro [UNESP]Borin, Thaiz FerrazMaschio-Signorini, Larissa BazelaMoschetta, Marina GobbeJardim-Perassi, Bruna VictorassoCalvinho, Guilherme BertoFacchini, Mariana CastilhoViloria-Petit, Alicia M.de Campos Zuccari, Debora Aparecida Pires [UNESP]2018-12-11T16:48:00Z2018-12-11T16:48:00Z2017-08-15info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article98-109application/pdfhttp://dx.doi.org/10.1016/j.lfs.2017.06.013Life Sciences, v. 183, p. 98-109.1879-06310024-3205http://hdl.handle.net/11449/16987810.1016/j.lfs.2017.06.0132-s2.0-850216701482-s2.0-85021670148.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengLife Sciences1,071info:eu-repo/semantics/openAccess2023-11-29T06:13:24Zoai:repositorio.unesp.br:11449/169878Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T19:01:25.697021Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Efficacy of melatonin, IL-25 and siIL-17B in tumorigenesis-associated properties of breast cancer cell lines |
title |
Efficacy of melatonin, IL-25 and siIL-17B in tumorigenesis-associated properties of breast cancer cell lines |
spellingShingle |
Efficacy of melatonin, IL-25 and siIL-17B in tumorigenesis-associated properties of breast cancer cell lines Gelaleti, Gabriela Bottaro [UNESP] Apoptosis Breast cancer Interleukin-17B Interleukin-17E Interleukin-25 Melatonin VEGF |
title_short |
Efficacy of melatonin, IL-25 and siIL-17B in tumorigenesis-associated properties of breast cancer cell lines |
title_full |
Efficacy of melatonin, IL-25 and siIL-17B in tumorigenesis-associated properties of breast cancer cell lines |
title_fullStr |
Efficacy of melatonin, IL-25 and siIL-17B in tumorigenesis-associated properties of breast cancer cell lines |
title_full_unstemmed |
Efficacy of melatonin, IL-25 and siIL-17B in tumorigenesis-associated properties of breast cancer cell lines |
title_sort |
Efficacy of melatonin, IL-25 and siIL-17B in tumorigenesis-associated properties of breast cancer cell lines |
author |
Gelaleti, Gabriela Bottaro [UNESP] |
author_facet |
Gelaleti, Gabriela Bottaro [UNESP] Borin, Thaiz Ferraz Maschio-Signorini, Larissa Bazela Moschetta, Marina Gobbe Jardim-Perassi, Bruna Victorasso Calvinho, Guilherme Berto Facchini, Mariana Castilho Viloria-Petit, Alicia M. de Campos Zuccari, Debora Aparecida Pires [UNESP] |
author_role |
author |
author2 |
Borin, Thaiz Ferraz Maschio-Signorini, Larissa Bazela Moschetta, Marina Gobbe Jardim-Perassi, Bruna Victorasso Calvinho, Guilherme Berto Facchini, Mariana Castilho Viloria-Petit, Alicia M. de Campos Zuccari, Debora Aparecida Pires [UNESP] |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Laboratório de Investigação Molecular do Câncer (LIMC) Augusta University University of Guelph |
dc.contributor.author.fl_str_mv |
Gelaleti, Gabriela Bottaro [UNESP] Borin, Thaiz Ferraz Maschio-Signorini, Larissa Bazela Moschetta, Marina Gobbe Jardim-Perassi, Bruna Victorasso Calvinho, Guilherme Berto Facchini, Mariana Castilho Viloria-Petit, Alicia M. de Campos Zuccari, Debora Aparecida Pires [UNESP] |
dc.subject.por.fl_str_mv |
Apoptosis Breast cancer Interleukin-17B Interleukin-17E Interleukin-25 Melatonin VEGF |
topic |
Apoptosis Breast cancer Interleukin-17B Interleukin-17E Interleukin-25 Melatonin VEGF |
description |
Mammary tumorigenesis can be modulated by melatonin, which has oncostatic action mediated by multiple mechanisms, including the inhibition of the activity of transcription factors such as NF-κB and modulation of interleukins (ILs) expression. IL-25 is an active cytokine that induces apoptosis in tumor cells due to differential expression of its receptor (IL-17RB). IL-17B competes with IL-25 for binding to IL-17RB in tumor cells, promoting tumorigenesis. This study purpose is to address the possibility of engaging IL-25/IL-17RB signaling to enhance the effect of melatonin on breast cancer cells. Breast cancer cell lines were cultured monolayers and 3D structures and treated with melatonin, IL-25, siIL-17B, each alone or in combination. Cell viability, gene and protein expression of caspase-3, cleaved caspase-3 and VEGF-A were performed by qPCR and immunofluorescence. In addition, an apoptosis membrane array was performed in metastatic cells. Treatments with melatonin and IL-25 significantly reduced tumor cells viability at 1 mM and 1 ng/mL, respectively, but did not alter cell viability of a non-tumorigenic epithelial cell line (MCF-10A). All treatments, alone and combined, significantly increased cleaved caspase-3 in tumor cells grown as monolayers and 3D structures (p < 0.05). Semi-quantitative analysis of apoptosis pathway proteins showed an increase of CYTO-C, DR6, IGFBP-3, IGFBP-5, IGFPB-6, IGF-1, IGF-1R, Livin, P21, P53, TNFRII, XIAP and hTRA proteins and reduction of caspase-3 (p < 0.05) after melatonin treatment. All treatments reduced VEGF-A protein expression in tumor cells (p < 0.05). Our results suggest therapeutic potential, with oncostatic effectiveness, pro-apoptotic and anti-angiogenic properties for melatonin and IL-25-driven signaling in breast cancer cells. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-08-15 2018-12-11T16:48:00Z 2018-12-11T16:48:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.lfs.2017.06.013 Life Sciences, v. 183, p. 98-109. 1879-0631 0024-3205 http://hdl.handle.net/11449/169878 10.1016/j.lfs.2017.06.013 2-s2.0-85021670148 2-s2.0-85021670148.pdf |
url |
http://dx.doi.org/10.1016/j.lfs.2017.06.013 http://hdl.handle.net/11449/169878 |
identifier_str_mv |
Life Sciences, v. 183, p. 98-109. 1879-0631 0024-3205 10.1016/j.lfs.2017.06.013 2-s2.0-85021670148 2-s2.0-85021670148.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Life Sciences 1,071 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
98-109 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1808129012651786240 |