Acid and alkaline phosphatase localization in the digestive tract mucosa of the Hemisorubim platyrhynchos
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.acthis.2016.08.001 http://hdl.handle.net/11449/173677 |
Resumo: | This cytochemical study investigated the acid and alkaline phosphatase of the digestive tract of Hemisorubim platyrhynchos. Acid phosphatase was detected in the lining epithelium throughout the digestive tract, whereas alkaline phosphatase was only observed in the intestine. In the esophagus, an acid phosphatase reaction occurred in the apical cytoplasm of the epithelial cells and was related to epithelial protection and freeing of superficial cells for sloughing. Similar results were also observed in epithelial cells of gastric epithelium. In the gastric glands, acid phosphatase occurred in lysosomes of the oxynticopeptic cells acting in the macromolecule degradation for use as an energy source, whereas in the vesiculotubular system, its presence could be related to secretion processes. Furthermore, acid phosphatase in the intestine occurred in microvilli and lysosomes of the enterocytes and was correlated to absorption and intracellular digestion. However, no difference was reported among the regions of the intestine. However, alkaline phosphatase reaction revealed a large number of reaction dots in the anterior intestine, with the number decreasing toward the posterior intestine. This enzyme has been related to several functions, highlighting its role in the nutrient absorption primarily in the anterior intestine but also being essential in pH regulation because this is a carnivorous species with many gastric glands with secretions that could damage the intestine. |
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Acid and alkaline phosphatase localization in the digestive tract mucosa of the Hemisorubim platyrhynchosCatfishCytochemistryEpitheliumTeleostUltrastructureThis cytochemical study investigated the acid and alkaline phosphatase of the digestive tract of Hemisorubim platyrhynchos. Acid phosphatase was detected in the lining epithelium throughout the digestive tract, whereas alkaline phosphatase was only observed in the intestine. In the esophagus, an acid phosphatase reaction occurred in the apical cytoplasm of the epithelial cells and was related to epithelial protection and freeing of superficial cells for sloughing. Similar results were also observed in epithelial cells of gastric epithelium. In the gastric glands, acid phosphatase occurred in lysosomes of the oxynticopeptic cells acting in the macromolecule degradation for use as an energy source, whereas in the vesiculotubular system, its presence could be related to secretion processes. Furthermore, acid phosphatase in the intestine occurred in microvilli and lysosomes of the enterocytes and was correlated to absorption and intracellular digestion. However, no difference was reported among the regions of the intestine. However, alkaline phosphatase reaction revealed a large number of reaction dots in the anterior intestine, with the number decreasing toward the posterior intestine. This enzyme has been related to several functions, highlighting its role in the nutrient absorption primarily in the anterior intestine but also being essential in pH regulation because this is a carnivorous species with many gastric glands with secretions that could damage the intestine.Federal University of Uberlândia – UFU Institute of Biomedical Sciences, 1720 Pará AvenueSão Paulo State University – UNESP Institute of Biosciences Letter and Exact Sciences, 2265 Cristovão Colombo StreetSão Paulo State University – UNESP Department of Biological Sciences Faculty of Sciences, 14-01 Engenheiro Luiz Edmundo Carrijo Coube AvenueSão Paulo State University – UNESP Aquaculture Center of UNESP – CAUNESP, Via de Acesso Prof. Paulo Donato Castellane, s/nSão Paulo State University – UNESP Institute of Biosciences Letter and Exact Sciences, 2265 Cristovão Colombo StreetSão Paulo State University – UNESP Department of Biological Sciences Faculty of Sciences, 14-01 Engenheiro Luiz Edmundo Carrijo Coube AvenueSão Paulo State University – UNESP Aquaculture Center of UNESP – CAUNESP, Via de Acesso Prof. Paulo Donato Castellane, s/nUniversidade Federal de Uberlândia (UFU)Universidade Estadual Paulista (Unesp)Faccioli, Claudemir Kuhn [UNESP]Chedid, Renata Alari [UNESP]Mori, Ricardo Hideo [UNESP]Amaral, Antônio Carlos do [UNESP]Franceschini-Vicentini, Irene Bastos [UNESP]Vicentini, Carlos Alberto [UNESP]2018-12-11T17:07:14Z2018-12-11T17:07:14Z2016-09-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article722-728application/pdfhttp://dx.doi.org/10.1016/j.acthis.2016.08.001Acta Histochemica, v. 118, n. 7, p. 722-728, 2016.1618-03720065-1281http://hdl.handle.net/11449/17367710.1016/j.acthis.2016.08.0012-s2.0-849923160202-s2.0-84992316020.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengActa Histochemica0,661info:eu-repo/semantics/openAccess2024-04-09T15:43:16Zoai:repositorio.unesp.br:11449/173677Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-04-09T15:43:16Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Acid and alkaline phosphatase localization in the digestive tract mucosa of the Hemisorubim platyrhynchos |
title |
Acid and alkaline phosphatase localization in the digestive tract mucosa of the Hemisorubim platyrhynchos |
spellingShingle |
Acid and alkaline phosphatase localization in the digestive tract mucosa of the Hemisorubim platyrhynchos Faccioli, Claudemir Kuhn [UNESP] Catfish Cytochemistry Epithelium Teleost Ultrastructure |
title_short |
Acid and alkaline phosphatase localization in the digestive tract mucosa of the Hemisorubim platyrhynchos |
title_full |
Acid and alkaline phosphatase localization in the digestive tract mucosa of the Hemisorubim platyrhynchos |
title_fullStr |
Acid and alkaline phosphatase localization in the digestive tract mucosa of the Hemisorubim platyrhynchos |
title_full_unstemmed |
Acid and alkaline phosphatase localization in the digestive tract mucosa of the Hemisorubim platyrhynchos |
title_sort |
Acid and alkaline phosphatase localization in the digestive tract mucosa of the Hemisorubim platyrhynchos |
author |
Faccioli, Claudemir Kuhn [UNESP] |
author_facet |
Faccioli, Claudemir Kuhn [UNESP] Chedid, Renata Alari [UNESP] Mori, Ricardo Hideo [UNESP] Amaral, Antônio Carlos do [UNESP] Franceschini-Vicentini, Irene Bastos [UNESP] Vicentini, Carlos Alberto [UNESP] |
author_role |
author |
author2 |
Chedid, Renata Alari [UNESP] Mori, Ricardo Hideo [UNESP] Amaral, Antônio Carlos do [UNESP] Franceschini-Vicentini, Irene Bastos [UNESP] Vicentini, Carlos Alberto [UNESP] |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal de Uberlândia (UFU) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Faccioli, Claudemir Kuhn [UNESP] Chedid, Renata Alari [UNESP] Mori, Ricardo Hideo [UNESP] Amaral, Antônio Carlos do [UNESP] Franceschini-Vicentini, Irene Bastos [UNESP] Vicentini, Carlos Alberto [UNESP] |
dc.subject.por.fl_str_mv |
Catfish Cytochemistry Epithelium Teleost Ultrastructure |
topic |
Catfish Cytochemistry Epithelium Teleost Ultrastructure |
description |
This cytochemical study investigated the acid and alkaline phosphatase of the digestive tract of Hemisorubim platyrhynchos. Acid phosphatase was detected in the lining epithelium throughout the digestive tract, whereas alkaline phosphatase was only observed in the intestine. In the esophagus, an acid phosphatase reaction occurred in the apical cytoplasm of the epithelial cells and was related to epithelial protection and freeing of superficial cells for sloughing. Similar results were also observed in epithelial cells of gastric epithelium. In the gastric glands, acid phosphatase occurred in lysosomes of the oxynticopeptic cells acting in the macromolecule degradation for use as an energy source, whereas in the vesiculotubular system, its presence could be related to secretion processes. Furthermore, acid phosphatase in the intestine occurred in microvilli and lysosomes of the enterocytes and was correlated to absorption and intracellular digestion. However, no difference was reported among the regions of the intestine. However, alkaline phosphatase reaction revealed a large number of reaction dots in the anterior intestine, with the number decreasing toward the posterior intestine. This enzyme has been related to several functions, highlighting its role in the nutrient absorption primarily in the anterior intestine but also being essential in pH regulation because this is a carnivorous species with many gastric glands with secretions that could damage the intestine. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-09-01 2018-12-11T17:07:14Z 2018-12-11T17:07:14Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.acthis.2016.08.001 Acta Histochemica, v. 118, n. 7, p. 722-728, 2016. 1618-0372 0065-1281 http://hdl.handle.net/11449/173677 10.1016/j.acthis.2016.08.001 2-s2.0-84992316020 2-s2.0-84992316020.pdf |
url |
http://dx.doi.org/10.1016/j.acthis.2016.08.001 http://hdl.handle.net/11449/173677 |
identifier_str_mv |
Acta Histochemica, v. 118, n. 7, p. 722-728, 2016. 1618-0372 0065-1281 10.1016/j.acthis.2016.08.001 2-s2.0-84992316020 2-s2.0-84992316020.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Acta Histochemica 0,661 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
722-728 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1803047258386595840 |