Analysis of the Virulence of an Atypical Enteropathogenic Escherichia coli Strain In Vitro and In Vivo and the Influence of Type Three Secretion System
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1155/2014/797508 http://hdl.handle.net/11449/111601 |
Resumo: | Atypical enteropathogenic Escherichia coli (aEPEC) inject various effectors into intestinal cells through a type three secretion system (T3SS), causing attaching and effacing (A/E) lesions. We investigated the role of T3SS in the ability of the aEPEC 1711-4 strain to interact with enterocytes in vitro (Caco-2 cells) and in vivo (rabbit ileal loops) and to translocate the rat intestinal mucosa in vivo. A T3SS isogenic mutant strain was constructed, which showed marked reduction in the ability to associate and invade but not to persist inside Caco-2 cells. After rabbit infection, only aEPEC 1711-4 was detected inside enterocytes at 8 and 24 hours pointing to a T3SS-dependent invasive potential in vivo. In contrast to aEPEC 1711-4, the T3SS-deficient strain no longer produced A/E lesions or induced macrophage infiltration. We also demonstrated that the ability of aEPEC 1711-4 to translocate through mesenteric lymph nodes to spleen and liver in a rat model depends on a functional T3SS, since a decreased number of T3SS mutant bacteria were recovered from extraintestinal sites. These findings indicate that the full virulence potential of aEPEC 1711-4 depends on a functional T3SS, which contributes to efficient adhesion/invasion in vitro and in vivo and to bacterial translocation to extraintestinal sites. |
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Analysis of the Virulence of an Atypical Enteropathogenic Escherichia coli Strain In Vitro and In Vivo and the Influence of Type Three Secretion SystemAtypical enteropathogenic Escherichia coli (aEPEC) inject various effectors into intestinal cells through a type three secretion system (T3SS), causing attaching and effacing (A/E) lesions. We investigated the role of T3SS in the ability of the aEPEC 1711-4 strain to interact with enterocytes in vitro (Caco-2 cells) and in vivo (rabbit ileal loops) and to translocate the rat intestinal mucosa in vivo. A T3SS isogenic mutant strain was constructed, which showed marked reduction in the ability to associate and invade but not to persist inside Caco-2 cells. After rabbit infection, only aEPEC 1711-4 was detected inside enterocytes at 8 and 24 hours pointing to a T3SS-dependent invasive potential in vivo. In contrast to aEPEC 1711-4, the T3SS-deficient strain no longer produced A/E lesions or induced macrophage infiltration. We also demonstrated that the ability of aEPEC 1711-4 to translocate through mesenteric lymph nodes to spleen and liver in a rat model depends on a functional T3SS, since a decreased number of T3SS mutant bacteria were recovered from extraintestinal sites. These findings indicate that the full virulence potential of aEPEC 1711-4 depends on a functional T3SS, which contributes to efficient adhesion/invasion in vitro and in vivo and to bacterial translocation to extraintestinal sites.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Univ Fed Sao Paulo, Escola Paulista Med, Dept Microbiol Imunol & Parasitol, BR-04023062 Sao Paulo, BrazilUniv Fed Sao Paulo, Escola Paulista Med, Dept Cirurgia, BR-04039032 Sao Paulo, BrazilUniv Sao Paulo, Fac Med Vet, Dept Ornitopatol, BR-05508900 Sao Paulo, BrazilUniv Estadual Paulista, Inst Biociencias, Dept Microbiol & Imunol, BR-18618970 Sao Paulo, BrazilFleury Med & Saude, BR-04344903 Sao Paulo, BrazilUniv Fed Sao Paulo, Escola Paulista Med, Ctr Microscopia Eletron, BR-04023062 Sao Paulo, BrazilUniv Estadual Paulista, Inst Biociencias, Dept Microbiol & Imunol, BR-18618970 Sao Paulo, BrazilFAPESP: 08/53812-4FAPESP: 11/12664-5CNPq: 304453/2011-0CNPq: 150833/2012-1Hindawi Publishing CorporationUniversidade Federal de São Paulo (UNIFESP)Universidade de São Paulo (USP)Universidade Estadual Paulista (Unesp)Fleury Med & SaudeSampaio, Suely C. F.Moreira, Fabiana C.Liberatore, Ana M. A.Vieira, Monica A. M.Knobl, TerezinhaRomao, Fabiano T.Hernandes, Rodrigo T. [UNESP]Ferreira, Claudete S. A.Ferreira, Antonio P.Felipe-Silva, AloisioSinigaglia-Coimbra, RitaKoh, Ivan H. J.Gomes, Tania A. T.2014-12-03T13:08:49Z2014-12-03T13:08:49Z2014-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article9application/pdfhttp://dx.doi.org/10.1155/2014/797508Biomed Research International. New York: Hindawi Publishing Corporation, 9 p., 2014.2314-6133http://hdl.handle.net/11449/11160110.1155/2014/797508WOS:000336293900001WOS000336293900001.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBioMed Research International2.5830,935info:eu-repo/semantics/openAccess2023-11-11T06:15:57Zoai:repositorio.unesp.br:11449/111601Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-11-11T06:15:57Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Analysis of the Virulence of an Atypical Enteropathogenic Escherichia coli Strain In Vitro and In Vivo and the Influence of Type Three Secretion System |
title |
Analysis of the Virulence of an Atypical Enteropathogenic Escherichia coli Strain In Vitro and In Vivo and the Influence of Type Three Secretion System |
spellingShingle |
Analysis of the Virulence of an Atypical Enteropathogenic Escherichia coli Strain In Vitro and In Vivo and the Influence of Type Three Secretion System Sampaio, Suely C. F. |
title_short |
Analysis of the Virulence of an Atypical Enteropathogenic Escherichia coli Strain In Vitro and In Vivo and the Influence of Type Three Secretion System |
title_full |
Analysis of the Virulence of an Atypical Enteropathogenic Escherichia coli Strain In Vitro and In Vivo and the Influence of Type Three Secretion System |
title_fullStr |
Analysis of the Virulence of an Atypical Enteropathogenic Escherichia coli Strain In Vitro and In Vivo and the Influence of Type Three Secretion System |
title_full_unstemmed |
Analysis of the Virulence of an Atypical Enteropathogenic Escherichia coli Strain In Vitro and In Vivo and the Influence of Type Three Secretion System |
title_sort |
Analysis of the Virulence of an Atypical Enteropathogenic Escherichia coli Strain In Vitro and In Vivo and the Influence of Type Three Secretion System |
author |
Sampaio, Suely C. F. |
author_facet |
Sampaio, Suely C. F. Moreira, Fabiana C. Liberatore, Ana M. A. Vieira, Monica A. M. Knobl, Terezinha Romao, Fabiano T. Hernandes, Rodrigo T. [UNESP] Ferreira, Claudete S. A. Ferreira, Antonio P. Felipe-Silva, Aloisio Sinigaglia-Coimbra, Rita Koh, Ivan H. J. Gomes, Tania A. T. |
author_role |
author |
author2 |
Moreira, Fabiana C. Liberatore, Ana M. A. Vieira, Monica A. M. Knobl, Terezinha Romao, Fabiano T. Hernandes, Rodrigo T. [UNESP] Ferreira, Claudete S. A. Ferreira, Antonio P. Felipe-Silva, Aloisio Sinigaglia-Coimbra, Rita Koh, Ivan H. J. Gomes, Tania A. T. |
author2_role |
author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal de São Paulo (UNIFESP) Universidade de São Paulo (USP) Universidade Estadual Paulista (Unesp) Fleury Med & Saude |
dc.contributor.author.fl_str_mv |
Sampaio, Suely C. F. Moreira, Fabiana C. Liberatore, Ana M. A. Vieira, Monica A. M. Knobl, Terezinha Romao, Fabiano T. Hernandes, Rodrigo T. [UNESP] Ferreira, Claudete S. A. Ferreira, Antonio P. Felipe-Silva, Aloisio Sinigaglia-Coimbra, Rita Koh, Ivan H. J. Gomes, Tania A. T. |
description |
Atypical enteropathogenic Escherichia coli (aEPEC) inject various effectors into intestinal cells through a type three secretion system (T3SS), causing attaching and effacing (A/E) lesions. We investigated the role of T3SS in the ability of the aEPEC 1711-4 strain to interact with enterocytes in vitro (Caco-2 cells) and in vivo (rabbit ileal loops) and to translocate the rat intestinal mucosa in vivo. A T3SS isogenic mutant strain was constructed, which showed marked reduction in the ability to associate and invade but not to persist inside Caco-2 cells. After rabbit infection, only aEPEC 1711-4 was detected inside enterocytes at 8 and 24 hours pointing to a T3SS-dependent invasive potential in vivo. In contrast to aEPEC 1711-4, the T3SS-deficient strain no longer produced A/E lesions or induced macrophage infiltration. We also demonstrated that the ability of aEPEC 1711-4 to translocate through mesenteric lymph nodes to spleen and liver in a rat model depends on a functional T3SS, since a decreased number of T3SS mutant bacteria were recovered from extraintestinal sites. These findings indicate that the full virulence potential of aEPEC 1711-4 depends on a functional T3SS, which contributes to efficient adhesion/invasion in vitro and in vivo and to bacterial translocation to extraintestinal sites. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-12-03T13:08:49Z 2014-12-03T13:08:49Z 2014-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1155/2014/797508 Biomed Research International. New York: Hindawi Publishing Corporation, 9 p., 2014. 2314-6133 http://hdl.handle.net/11449/111601 10.1155/2014/797508 WOS:000336293900001 WOS000336293900001.pdf |
url |
http://dx.doi.org/10.1155/2014/797508 http://hdl.handle.net/11449/111601 |
identifier_str_mv |
Biomed Research International. New York: Hindawi Publishing Corporation, 9 p., 2014. 2314-6133 10.1155/2014/797508 WOS:000336293900001 WOS000336293900001.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
BioMed Research International 2.583 0,935 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
9 application/pdf |
dc.publisher.none.fl_str_mv |
Hindawi Publishing Corporation |
publisher.none.fl_str_mv |
Hindawi Publishing Corporation |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1792961796596826112 |