Uncovering the evolutionary history of neo-XY sex chromosomes in the grasshopper Ronderosia bergii (Orthoptera, Melanoplinae) through satellite DNA analysis

Detalhes bibliográficos
Autor(a) principal: Palacios-Gimenez, Octavio M. [UNESP]
Data de Publicação: 2018
Outros Autores: Milani, Diogo [UNESP], Lemos, Bernardo, Castillo, Elio R., Martí, Dardo A., Ramos, Erica [UNESP], Martins, Cesar [UNESP], Cabral-De-Mello, Diogo C. [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1186/s12862-017-1113-x
http://hdl.handle.net/11449/179503
Resumo: Background: Neo-sex chromosome systems arose independently multiple times in evolution, presenting the remarkable characteristic of repetitive DNAs accumulation. Among grasshoppers, occurrence of neo-XY was repeatedly noticed in Melanoplinae. Here we analyzed the most abundant tandem repeats of R. bergii (2n = 22, neo-XY♂) using deep Illumina sequencing and graph-based clustering in order to address the neo-sex chromosomes evolution. Results: The analyses revealed ten families of satDNAs comprising about ~1% of the male genome, which occupied mainly C-positive regions of autosomes. Regarding the sex chromosomes, satDNAs were recorded within centromeric or interstitial regions of the neo-X chromosome and four satDNAs occurred in the neo-Y, two of them being exclusive (Rber248 and Rber299). Using a combination of probes we uncovered five well-defined cytological variants for neo-Y, originated by multiple paracentric inversions and satDNA amplification, besides fragmented neo-Y. These neo-Y variants were distinct in frequency between embryos and adult males. Conclusions: The genomic data together with cytogenetic mapping enabled us to better understand the neo-sex chromosome dynamics in grasshoppers, reinforcing differentiation of neo-X and neo-Y and revealing the occurrence of multiple additional rearrangements involved in the neo-Y evolution of R. bergii. We discussed the possible causes that led to differences in frequency for the neo-Y variants between embryos and adults. Finally we hypothesize about the role of DNA satellites in R. bergii as well as putative historical events involved in the evolution of the R. bergii neo-XY.
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spelling Uncovering the evolutionary history of neo-XY sex chromosomes in the grasshopper Ronderosia bergii (Orthoptera, Melanoplinae) through satellite DNA analysisChromosomal rearrangementsEvolutionFISHSatellite DNASex chromosomeBackground: Neo-sex chromosome systems arose independently multiple times in evolution, presenting the remarkable characteristic of repetitive DNAs accumulation. Among grasshoppers, occurrence of neo-XY was repeatedly noticed in Melanoplinae. Here we analyzed the most abundant tandem repeats of R. bergii (2n = 22, neo-XY♂) using deep Illumina sequencing and graph-based clustering in order to address the neo-sex chromosomes evolution. Results: The analyses revealed ten families of satDNAs comprising about ~1% of the male genome, which occupied mainly C-positive regions of autosomes. Regarding the sex chromosomes, satDNAs were recorded within centromeric or interstitial regions of the neo-X chromosome and four satDNAs occurred in the neo-Y, two of them being exclusive (Rber248 and Rber299). Using a combination of probes we uncovered five well-defined cytological variants for neo-Y, originated by multiple paracentric inversions and satDNA amplification, besides fragmented neo-Y. These neo-Y variants were distinct in frequency between embryos and adult males. Conclusions: The genomic data together with cytogenetic mapping enabled us to better understand the neo-sex chromosome dynamics in grasshoppers, reinforcing differentiation of neo-X and neo-Y and revealing the occurrence of multiple additional rearrangements involved in the neo-Y evolution of R. bergii. We discussed the possible causes that led to differences in frequency for the neo-Y variants between embryos and adults. Finally we hypothesize about the role of DNA satellites in R. bergii as well as putative historical events involved in the evolution of the R. bergii neo-XY.Departamento de Biologia UNESP-Univ Estadual Paulista Instituto de Biociências/IBProgram in Molecular and Integrative Physiological Sciences Department of Environmental Health Harvard University T. H. Chan School of Public HealthIBS UNaM CONICETDepartamento de Morfologia UNESP-Univ Estadual Paulista Instituto de Biociências/IBDepartamento de Biologia UNESP-Univ Estadual Paulista Instituto de Biociências/IBDepartamento de Morfologia UNESP-Univ Estadual Paulista Instituto de Biociências/IBUniversidade Estadual Paulista (Unesp)T. H. Chan School of Public HealthCONICETPalacios-Gimenez, Octavio M. [UNESP]Milani, Diogo [UNESP]Lemos, BernardoCastillo, Elio R.Martí, Dardo A.Ramos, Erica [UNESP]Martins, Cesar [UNESP]Cabral-De-Mello, Diogo C. [UNESP]2018-12-11T17:35:26Z2018-12-11T17:35:26Z2018-01-08info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://dx.doi.org/10.1186/s12862-017-1113-xBMC Evolutionary Biology, v. 18, n. 1, 2018.1471-2148http://hdl.handle.net/11449/17950310.1186/s12862-017-1113-x2-s2.0-850405229882-s2.0-85040522988.pdf88588006994253520000-0003-3534-974XScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBMC Evolutionary Biology1,656info:eu-repo/semantics/openAccess2024-01-06T06:23:24Zoai:repositorio.unesp.br:11449/179503Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T22:13:52.095267Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Uncovering the evolutionary history of neo-XY sex chromosomes in the grasshopper Ronderosia bergii (Orthoptera, Melanoplinae) through satellite DNA analysis
title Uncovering the evolutionary history of neo-XY sex chromosomes in the grasshopper Ronderosia bergii (Orthoptera, Melanoplinae) through satellite DNA analysis
spellingShingle Uncovering the evolutionary history of neo-XY sex chromosomes in the grasshopper Ronderosia bergii (Orthoptera, Melanoplinae) through satellite DNA analysis
Palacios-Gimenez, Octavio M. [UNESP]
Chromosomal rearrangements
Evolution
FISH
Satellite DNA
Sex chromosome
title_short Uncovering the evolutionary history of neo-XY sex chromosomes in the grasshopper Ronderosia bergii (Orthoptera, Melanoplinae) through satellite DNA analysis
title_full Uncovering the evolutionary history of neo-XY sex chromosomes in the grasshopper Ronderosia bergii (Orthoptera, Melanoplinae) through satellite DNA analysis
title_fullStr Uncovering the evolutionary history of neo-XY sex chromosomes in the grasshopper Ronderosia bergii (Orthoptera, Melanoplinae) through satellite DNA analysis
title_full_unstemmed Uncovering the evolutionary history of neo-XY sex chromosomes in the grasshopper Ronderosia bergii (Orthoptera, Melanoplinae) through satellite DNA analysis
title_sort Uncovering the evolutionary history of neo-XY sex chromosomes in the grasshopper Ronderosia bergii (Orthoptera, Melanoplinae) through satellite DNA analysis
author Palacios-Gimenez, Octavio M. [UNESP]
author_facet Palacios-Gimenez, Octavio M. [UNESP]
Milani, Diogo [UNESP]
Lemos, Bernardo
Castillo, Elio R.
Martí, Dardo A.
Ramos, Erica [UNESP]
Martins, Cesar [UNESP]
Cabral-De-Mello, Diogo C. [UNESP]
author_role author
author2 Milani, Diogo [UNESP]
Lemos, Bernardo
Castillo, Elio R.
Martí, Dardo A.
Ramos, Erica [UNESP]
Martins, Cesar [UNESP]
Cabral-De-Mello, Diogo C. [UNESP]
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
T. H. Chan School of Public Health
CONICET
dc.contributor.author.fl_str_mv Palacios-Gimenez, Octavio M. [UNESP]
Milani, Diogo [UNESP]
Lemos, Bernardo
Castillo, Elio R.
Martí, Dardo A.
Ramos, Erica [UNESP]
Martins, Cesar [UNESP]
Cabral-De-Mello, Diogo C. [UNESP]
dc.subject.por.fl_str_mv Chromosomal rearrangements
Evolution
FISH
Satellite DNA
Sex chromosome
topic Chromosomal rearrangements
Evolution
FISH
Satellite DNA
Sex chromosome
description Background: Neo-sex chromosome systems arose independently multiple times in evolution, presenting the remarkable characteristic of repetitive DNAs accumulation. Among grasshoppers, occurrence of neo-XY was repeatedly noticed in Melanoplinae. Here we analyzed the most abundant tandem repeats of R. bergii (2n = 22, neo-XY♂) using deep Illumina sequencing and graph-based clustering in order to address the neo-sex chromosomes evolution. Results: The analyses revealed ten families of satDNAs comprising about ~1% of the male genome, which occupied mainly C-positive regions of autosomes. Regarding the sex chromosomes, satDNAs were recorded within centromeric or interstitial regions of the neo-X chromosome and four satDNAs occurred in the neo-Y, two of them being exclusive (Rber248 and Rber299). Using a combination of probes we uncovered five well-defined cytological variants for neo-Y, originated by multiple paracentric inversions and satDNA amplification, besides fragmented neo-Y. These neo-Y variants were distinct in frequency between embryos and adult males. Conclusions: The genomic data together with cytogenetic mapping enabled us to better understand the neo-sex chromosome dynamics in grasshoppers, reinforcing differentiation of neo-X and neo-Y and revealing the occurrence of multiple additional rearrangements involved in the neo-Y evolution of R. bergii. We discussed the possible causes that led to differences in frequency for the neo-Y variants between embryos and adults. Finally we hypothesize about the role of DNA satellites in R. bergii as well as putative historical events involved in the evolution of the R. bergii neo-XY.
publishDate 2018
dc.date.none.fl_str_mv 2018-12-11T17:35:26Z
2018-12-11T17:35:26Z
2018-01-08
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1186/s12862-017-1113-x
BMC Evolutionary Biology, v. 18, n. 1, 2018.
1471-2148
http://hdl.handle.net/11449/179503
10.1186/s12862-017-1113-x
2-s2.0-85040522988
2-s2.0-85040522988.pdf
8858800699425352
0000-0003-3534-974X
url http://dx.doi.org/10.1186/s12862-017-1113-x
http://hdl.handle.net/11449/179503
identifier_str_mv BMC Evolutionary Biology, v. 18, n. 1, 2018.
1471-2148
10.1186/s12862-017-1113-x
2-s2.0-85040522988
2-s2.0-85040522988.pdf
8858800699425352
0000-0003-3534-974X
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv BMC Evolutionary Biology
1,656
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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