Ribotyping and virulence markers of Yersinia pseudotuberculosis strains isolated from animals in Brazil
Autor(a) principal: | |
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Data de Publicação: | 2007 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/S0074-02762007005000072 http://hdl.handle.net/11449/8022 |
Resumo: | Ribotyping and virulence markers has been used to investigate 68 Yersinia pseudotuberculosis strains of serogroups O:1a and O:3. The strains were isolated from clinical material obtained from healthy and sick animals in the Southern region of Brazil. Ribotypes were identified by double digestion of extracted DNA with the restriction endonucleases SmaI and PstI, separation by electrophoresis and hybridization with a digoxigenin-labeled cDNA probe. The presence of the chromosomal virulence marker genes inv, irp1, irp2, psn, ybtE, ybtP-ybtQ, and ybtX-ybtS, of the IS100 insertion sequence, and of the plasmid gene lcrF was detected by polymerase chain reaction. The strains were grouped into four distinct ribotypes, all of them comprising several strains. Ribotypes 1 and 4 presented distinct profiles, with 57.3% genetic similarity, ribotypes 2 and 3 presented 52.5% genetic similarity, and genetic similarity was 45% between these two groups (1/4 and 2/3). All strains possessed the inv, irp1, and irp2 genes. Additionally, strains of serogroup O:1a carried psn, ybtE, ybtP-ybtQ, ybtX-ybtS, and IS100. As expected lcrF was only detected in strains harboring the virulence plasmid. These data demonstrate the presence of Y. pseudotuberculosis strains harboring genotypic virulence markers in the livestock from Southern Brazil and that the dissemination of these bacteria may occur between herds. |
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Ribotyping and virulence markers of Yersinia pseudotuberculosis strains isolated from animals in BrazilribotypingYersinia pseudotuberculosisvirulence markerspolymerase chain reactionRibotyping and virulence markers has been used to investigate 68 Yersinia pseudotuberculosis strains of serogroups O:1a and O:3. The strains were isolated from clinical material obtained from healthy and sick animals in the Southern region of Brazil. Ribotypes were identified by double digestion of extracted DNA with the restriction endonucleases SmaI and PstI, separation by electrophoresis and hybridization with a digoxigenin-labeled cDNA probe. The presence of the chromosomal virulence marker genes inv, irp1, irp2, psn, ybtE, ybtP-ybtQ, and ybtX-ybtS, of the IS100 insertion sequence, and of the plasmid gene lcrF was detected by polymerase chain reaction. The strains were grouped into four distinct ribotypes, all of them comprising several strains. Ribotypes 1 and 4 presented distinct profiles, with 57.3% genetic similarity, ribotypes 2 and 3 presented 52.5% genetic similarity, and genetic similarity was 45% between these two groups (1/4 and 2/3). All strains possessed the inv, irp1, and irp2 genes. Additionally, strains of serogroup O:1a carried psn, ybtE, ybtP-ybtQ, ybtX-ybtS, and IS100. As expected lcrF was only detected in strains harboring the virulence plasmid. These data demonstrate the presence of Y. pseudotuberculosis strains harboring genotypic virulence markers in the livestock from Southern Brazil and that the dissemination of these bacteria may occur between herds.Universidade de Franca Laboratório de Pesquisa em Microbiologia AplicadaUniversidade Estadual Paulista Faculdade de Ciências Farmacêuticas Departamento de Ciências BiológicasUniversidade Estadual Paulista Faculdade de Ciências Farmacêuticas Departamento de Ciências BiológicasInstituto Oswaldo Cruz, Ministério da SaúdeUniversidade de Franca Laboratório de Pesquisa em Microbiologia AplicadaUniversidade Estadual Paulista (Unesp)Martins, Carlos Henrique GomesBauab, Taís Maria [UNESP]Leite, Clarice Queico Fujimura [UNESP]Falcão, Deise Pasetto [UNESP]2014-05-20T13:25:21Z2014-05-20T13:25:21Z2007-08-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article587-592application/pdfhttp://dx.doi.org/10.1590/S0074-02762007005000072Memórias do Instituto Oswaldo Cruz. Instituto Oswaldo Cruz, Ministério da Saúde, v. 102, n. 5, p. 587-592, 2007.0074-0276http://hdl.handle.net/11449/802210.1590/S0074-02762007005000072S0074-02762007000500008S0074-02762007000500008.pdf49107548382775802114570774349859SciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMemórias do Instituto Oswaldo Cruz2.8331,172info:eu-repo/semantics/openAccess2024-06-24T13:08:36Zoai:repositorio.unesp.br:11449/8022Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-06T00:04:51.168923Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Ribotyping and virulence markers of Yersinia pseudotuberculosis strains isolated from animals in Brazil |
title |
Ribotyping and virulence markers of Yersinia pseudotuberculosis strains isolated from animals in Brazil |
spellingShingle |
Ribotyping and virulence markers of Yersinia pseudotuberculosis strains isolated from animals in Brazil Martins, Carlos Henrique Gomes ribotyping Yersinia pseudotuberculosis virulence markers polymerase chain reaction |
title_short |
Ribotyping and virulence markers of Yersinia pseudotuberculosis strains isolated from animals in Brazil |
title_full |
Ribotyping and virulence markers of Yersinia pseudotuberculosis strains isolated from animals in Brazil |
title_fullStr |
Ribotyping and virulence markers of Yersinia pseudotuberculosis strains isolated from animals in Brazil |
title_full_unstemmed |
Ribotyping and virulence markers of Yersinia pseudotuberculosis strains isolated from animals in Brazil |
title_sort |
Ribotyping and virulence markers of Yersinia pseudotuberculosis strains isolated from animals in Brazil |
author |
Martins, Carlos Henrique Gomes |
author_facet |
Martins, Carlos Henrique Gomes Bauab, Taís Maria [UNESP] Leite, Clarice Queico Fujimura [UNESP] Falcão, Deise Pasetto [UNESP] |
author_role |
author |
author2 |
Bauab, Taís Maria [UNESP] Leite, Clarice Queico Fujimura [UNESP] Falcão, Deise Pasetto [UNESP] |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Universidade de Franca Laboratório de Pesquisa em Microbiologia Aplicada Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Martins, Carlos Henrique Gomes Bauab, Taís Maria [UNESP] Leite, Clarice Queico Fujimura [UNESP] Falcão, Deise Pasetto [UNESP] |
dc.subject.por.fl_str_mv |
ribotyping Yersinia pseudotuberculosis virulence markers polymerase chain reaction |
topic |
ribotyping Yersinia pseudotuberculosis virulence markers polymerase chain reaction |
description |
Ribotyping and virulence markers has been used to investigate 68 Yersinia pseudotuberculosis strains of serogroups O:1a and O:3. The strains were isolated from clinical material obtained from healthy and sick animals in the Southern region of Brazil. Ribotypes were identified by double digestion of extracted DNA with the restriction endonucleases SmaI and PstI, separation by electrophoresis and hybridization with a digoxigenin-labeled cDNA probe. The presence of the chromosomal virulence marker genes inv, irp1, irp2, psn, ybtE, ybtP-ybtQ, and ybtX-ybtS, of the IS100 insertion sequence, and of the plasmid gene lcrF was detected by polymerase chain reaction. The strains were grouped into four distinct ribotypes, all of them comprising several strains. Ribotypes 1 and 4 presented distinct profiles, with 57.3% genetic similarity, ribotypes 2 and 3 presented 52.5% genetic similarity, and genetic similarity was 45% between these two groups (1/4 and 2/3). All strains possessed the inv, irp1, and irp2 genes. Additionally, strains of serogroup O:1a carried psn, ybtE, ybtP-ybtQ, ybtX-ybtS, and IS100. As expected lcrF was only detected in strains harboring the virulence plasmid. These data demonstrate the presence of Y. pseudotuberculosis strains harboring genotypic virulence markers in the livestock from Southern Brazil and that the dissemination of these bacteria may occur between herds. |
publishDate |
2007 |
dc.date.none.fl_str_mv |
2007-08-01 2014-05-20T13:25:21Z 2014-05-20T13:25:21Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S0074-02762007005000072 Memórias do Instituto Oswaldo Cruz. Instituto Oswaldo Cruz, Ministério da Saúde, v. 102, n. 5, p. 587-592, 2007. 0074-0276 http://hdl.handle.net/11449/8022 10.1590/S0074-02762007005000072 S0074-02762007000500008 S0074-02762007000500008.pdf 4910754838277580 2114570774349859 |
url |
http://dx.doi.org/10.1590/S0074-02762007005000072 http://hdl.handle.net/11449/8022 |
identifier_str_mv |
Memórias do Instituto Oswaldo Cruz. Instituto Oswaldo Cruz, Ministério da Saúde, v. 102, n. 5, p. 587-592, 2007. 0074-0276 10.1590/S0074-02762007005000072 S0074-02762007000500008 S0074-02762007000500008.pdf 4910754838277580 2114570774349859 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Memórias do Instituto Oswaldo Cruz 2.833 1,172 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
587-592 application/pdf |
dc.publisher.none.fl_str_mv |
Instituto Oswaldo Cruz, Ministério da Saúde |
publisher.none.fl_str_mv |
Instituto Oswaldo Cruz, Ministério da Saúde |
dc.source.none.fl_str_mv |
SciELO reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808129580622413824 |