Ribotyping and virulence markers of Yersinia pseudotuberculosis strains isolated from animals in Brazil

Detalhes bibliográficos
Autor(a) principal: Martins, Carlos Henrique Gomes
Data de Publicação: 2007
Outros Autores: Bauab, Taís Maria [UNESP], Leite, Clarice Queico Fujimura [UNESP], Falcão, Deise Pasetto [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1590/S0074-02762007005000072
http://hdl.handle.net/11449/8022
Resumo: Ribotyping and virulence markers has been used to investigate 68 Yersinia pseudotuberculosis strains of serogroups O:1a and O:3. The strains were isolated from clinical material obtained from healthy and sick animals in the Southern region of Brazil. Ribotypes were identified by double digestion of extracted DNA with the restriction endonucleases SmaI and PstI, separation by electrophoresis and hybridization with a digoxigenin-labeled cDNA probe. The presence of the chromosomal virulence marker genes inv, irp1, irp2, psn, ybtE, ybtP-ybtQ, and ybtX-ybtS, of the IS100 insertion sequence, and of the plasmid gene lcrF was detected by polymerase chain reaction. The strains were grouped into four distinct ribotypes, all of them comprising several strains. Ribotypes 1 and 4 presented distinct profiles, with 57.3% genetic similarity, ribotypes 2 and 3 presented 52.5% genetic similarity, and genetic similarity was 45% between these two groups (1/4 and 2/3). All strains possessed the inv, irp1, and irp2 genes. Additionally, strains of serogroup O:1a carried psn, ybtE, ybtP-ybtQ, ybtX-ybtS, and IS100. As expected lcrF was only detected in strains harboring the virulence plasmid. These data demonstrate the presence of Y. pseudotuberculosis strains harboring genotypic virulence markers in the livestock from Southern Brazil and that the dissemination of these bacteria may occur between herds.
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spelling Ribotyping and virulence markers of Yersinia pseudotuberculosis strains isolated from animals in BrazilribotypingYersinia pseudotuberculosisvirulence markerspolymerase chain reactionRibotyping and virulence markers has been used to investigate 68 Yersinia pseudotuberculosis strains of serogroups O:1a and O:3. The strains were isolated from clinical material obtained from healthy and sick animals in the Southern region of Brazil. Ribotypes were identified by double digestion of extracted DNA with the restriction endonucleases SmaI and PstI, separation by electrophoresis and hybridization with a digoxigenin-labeled cDNA probe. The presence of the chromosomal virulence marker genes inv, irp1, irp2, psn, ybtE, ybtP-ybtQ, and ybtX-ybtS, of the IS100 insertion sequence, and of the plasmid gene lcrF was detected by polymerase chain reaction. The strains were grouped into four distinct ribotypes, all of them comprising several strains. Ribotypes 1 and 4 presented distinct profiles, with 57.3% genetic similarity, ribotypes 2 and 3 presented 52.5% genetic similarity, and genetic similarity was 45% between these two groups (1/4 and 2/3). All strains possessed the inv, irp1, and irp2 genes. Additionally, strains of serogroup O:1a carried psn, ybtE, ybtP-ybtQ, ybtX-ybtS, and IS100. As expected lcrF was only detected in strains harboring the virulence plasmid. These data demonstrate the presence of Y. pseudotuberculosis strains harboring genotypic virulence markers in the livestock from Southern Brazil and that the dissemination of these bacteria may occur between herds.Universidade de Franca Laboratório de Pesquisa em Microbiologia AplicadaUniversidade Estadual Paulista Faculdade de Ciências Farmacêuticas Departamento de Ciências BiológicasUniversidade Estadual Paulista Faculdade de Ciências Farmacêuticas Departamento de Ciências BiológicasInstituto Oswaldo Cruz, Ministério da SaúdeUniversidade de Franca Laboratório de Pesquisa em Microbiologia AplicadaUniversidade Estadual Paulista (Unesp)Martins, Carlos Henrique GomesBauab, Taís Maria [UNESP]Leite, Clarice Queico Fujimura [UNESP]Falcão, Deise Pasetto [UNESP]2014-05-20T13:25:21Z2014-05-20T13:25:21Z2007-08-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article587-592application/pdfhttp://dx.doi.org/10.1590/S0074-02762007005000072Memórias do Instituto Oswaldo Cruz. Instituto Oswaldo Cruz, Ministério da Saúde, v. 102, n. 5, p. 587-592, 2007.0074-0276http://hdl.handle.net/11449/802210.1590/S0074-02762007005000072S0074-02762007000500008S0074-02762007000500008.pdf49107548382775802114570774349859SciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMemórias do Instituto Oswaldo Cruz2.8331,172info:eu-repo/semantics/openAccess2024-06-24T13:08:36Zoai:repositorio.unesp.br:11449/8022Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-06T00:04:51.168923Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Ribotyping and virulence markers of Yersinia pseudotuberculosis strains isolated from animals in Brazil
title Ribotyping and virulence markers of Yersinia pseudotuberculosis strains isolated from animals in Brazil
spellingShingle Ribotyping and virulence markers of Yersinia pseudotuberculosis strains isolated from animals in Brazil
Martins, Carlos Henrique Gomes
ribotyping
Yersinia pseudotuberculosis
virulence markers
polymerase chain reaction
title_short Ribotyping and virulence markers of Yersinia pseudotuberculosis strains isolated from animals in Brazil
title_full Ribotyping and virulence markers of Yersinia pseudotuberculosis strains isolated from animals in Brazil
title_fullStr Ribotyping and virulence markers of Yersinia pseudotuberculosis strains isolated from animals in Brazil
title_full_unstemmed Ribotyping and virulence markers of Yersinia pseudotuberculosis strains isolated from animals in Brazil
title_sort Ribotyping and virulence markers of Yersinia pseudotuberculosis strains isolated from animals in Brazil
author Martins, Carlos Henrique Gomes
author_facet Martins, Carlos Henrique Gomes
Bauab, Taís Maria [UNESP]
Leite, Clarice Queico Fujimura [UNESP]
Falcão, Deise Pasetto [UNESP]
author_role author
author2 Bauab, Taís Maria [UNESP]
Leite, Clarice Queico Fujimura [UNESP]
Falcão, Deise Pasetto [UNESP]
author2_role author
author
author
dc.contributor.none.fl_str_mv Universidade de Franca Laboratório de Pesquisa em Microbiologia Aplicada
Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Martins, Carlos Henrique Gomes
Bauab, Taís Maria [UNESP]
Leite, Clarice Queico Fujimura [UNESP]
Falcão, Deise Pasetto [UNESP]
dc.subject.por.fl_str_mv ribotyping
Yersinia pseudotuberculosis
virulence markers
polymerase chain reaction
topic ribotyping
Yersinia pseudotuberculosis
virulence markers
polymerase chain reaction
description Ribotyping and virulence markers has been used to investigate 68 Yersinia pseudotuberculosis strains of serogroups O:1a and O:3. The strains were isolated from clinical material obtained from healthy and sick animals in the Southern region of Brazil. Ribotypes were identified by double digestion of extracted DNA with the restriction endonucleases SmaI and PstI, separation by electrophoresis and hybridization with a digoxigenin-labeled cDNA probe. The presence of the chromosomal virulence marker genes inv, irp1, irp2, psn, ybtE, ybtP-ybtQ, and ybtX-ybtS, of the IS100 insertion sequence, and of the plasmid gene lcrF was detected by polymerase chain reaction. The strains were grouped into four distinct ribotypes, all of them comprising several strains. Ribotypes 1 and 4 presented distinct profiles, with 57.3% genetic similarity, ribotypes 2 and 3 presented 52.5% genetic similarity, and genetic similarity was 45% between these two groups (1/4 and 2/3). All strains possessed the inv, irp1, and irp2 genes. Additionally, strains of serogroup O:1a carried psn, ybtE, ybtP-ybtQ, ybtX-ybtS, and IS100. As expected lcrF was only detected in strains harboring the virulence plasmid. These data demonstrate the presence of Y. pseudotuberculosis strains harboring genotypic virulence markers in the livestock from Southern Brazil and that the dissemination of these bacteria may occur between herds.
publishDate 2007
dc.date.none.fl_str_mv 2007-08-01
2014-05-20T13:25:21Z
2014-05-20T13:25:21Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S0074-02762007005000072
Memórias do Instituto Oswaldo Cruz. Instituto Oswaldo Cruz, Ministério da Saúde, v. 102, n. 5, p. 587-592, 2007.
0074-0276
http://hdl.handle.net/11449/8022
10.1590/S0074-02762007005000072
S0074-02762007000500008
S0074-02762007000500008.pdf
4910754838277580
2114570774349859
url http://dx.doi.org/10.1590/S0074-02762007005000072
http://hdl.handle.net/11449/8022
identifier_str_mv Memórias do Instituto Oswaldo Cruz. Instituto Oswaldo Cruz, Ministério da Saúde, v. 102, n. 5, p. 587-592, 2007.
0074-0276
10.1590/S0074-02762007005000072
S0074-02762007000500008
S0074-02762007000500008.pdf
4910754838277580
2114570774349859
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Memórias do Instituto Oswaldo Cruz
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dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 587-592
application/pdf
dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
dc.source.none.fl_str_mv SciELO
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
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