Analysis of Toll-Like Receptors, iNOS and Cytokine Profiles in Patients with Pulmonary Tuberculosis during Anti-Tuberculosis Treatment
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1371/journal.pone.0088572 http://hdl.handle.net/11449/112227 |
Resumo: | Toll-like receptors (TLRs) play an important role in mycobacterial infection, although little is known about the roles of these receptors, cytokines and nitric oxide during anti-tuberculosis treatment. Our objective was to evaluate the mRNA and cell surface expression of TLR2 and TLR4; inducible nitric oxide synthase (iNOS) expression; and cytokine Th1, Th2 and Th17 profiles in pulmonary tuberculosis patients at different time points of anti-tuberculosis treatment. Peripheral blood mononuclear cells (PBMCs) were obtained from PPD+ healthy controls and from patients receiving anti-tuberculosis treatment. Gene expression quantification was performed by qPCR, cell surface expression was assessed using flow cytometry, and cytokine quantification was conducted using the CBA technique. The treated patients presented higher gene expression and higher numbers of receptors on the cell surface of lymphocytes and monocytes than did control individuals. IL-12 and IFN-gamma levels increased after the start of treatment, whereas TNF-alpha levels were reduced. TGF-beta presented the highest levels during treatment. IL-10 and IL-17 expression and production tended to increase during treatment. iNOS gene expression was reduced throughout treatment in patients. Our results suggest that anti-tuberculosis treatment modulates the immune response, inducing an increase in the expression of TLRs and pro-and anti-inflammatory cytokines to combat bacteria and reduce the inflammatory process. |
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Analysis of Toll-Like Receptors, iNOS and Cytokine Profiles in Patients with Pulmonary Tuberculosis during Anti-Tuberculosis TreatmentToll-like receptors (TLRs) play an important role in mycobacterial infection, although little is known about the roles of these receptors, cytokines and nitric oxide during anti-tuberculosis treatment. Our objective was to evaluate the mRNA and cell surface expression of TLR2 and TLR4; inducible nitric oxide synthase (iNOS) expression; and cytokine Th1, Th2 and Th17 profiles in pulmonary tuberculosis patients at different time points of anti-tuberculosis treatment. Peripheral blood mononuclear cells (PBMCs) were obtained from PPD+ healthy controls and from patients receiving anti-tuberculosis treatment. Gene expression quantification was performed by qPCR, cell surface expression was assessed using flow cytometry, and cytokine quantification was conducted using the CBA technique. The treated patients presented higher gene expression and higher numbers of receptors on the cell surface of lymphocytes and monocytes than did control individuals. IL-12 and IFN-gamma levels increased after the start of treatment, whereas TNF-alpha levels were reduced. TGF-beta presented the highest levels during treatment. IL-10 and IL-17 expression and production tended to increase during treatment. iNOS gene expression was reduced throughout treatment in patients. Our results suggest that anti-tuberculosis treatment modulates the immune response, inducing an increase in the expression of TLRs and pro-and anti-inflammatory cytokines to combat bacteria and reduce the inflammatory process.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Univ Estadual Paulista, Dept Trop Dis, Botucatu Sch Med, Sao Paulo, BrazilUniv Estadual Paulista, Flow Cytometry Lab, Botucatu Sch Med, Hemoctr, Sao Paulo, BrazilUniv Estadual Paulista, Biosci Inst, Dept Microbiol & Immunol, Sao Paulo, BrazilUniv Estadual Paulista, Dept Nursing, Botucatu Sch Med, Sao Paulo, BrazilUniv Estadual Paulista, Dermatol & Radiotherapy Dept, Botucatu Sch Med, Sao Paulo, BrazilUniv Estadual Paulista, Dept Trop Dis, Botucatu Sch Med, Sao Paulo, BrazilUniv Estadual Paulista, Flow Cytometry Lab, Botucatu Sch Med, Hemoctr, Sao Paulo, BrazilUniv Estadual Paulista, Biosci Inst, Dept Microbiol & Immunol, Sao Paulo, BrazilUniv Estadual Paulista, Dept Nursing, Botucatu Sch Med, Sao Paulo, BrazilUniv Estadual Paulista, Dermatol & Radiotherapy Dept, Botucatu Sch Med, Sao Paulo, BrazilPublic Library ScienceUniversidade Estadual Paulista (Unesp)Cardoso de Oliveira, Larissa Ragozo [UNESP]Peresi, Eliana [UNESP]Golim, Márjorie de Assis [UNESP]Gatto, Mariana [UNESP]Araujo Junior, Joao Pessoa [UNESP]Pellison Nunes da Costa, Erika Alessandra [UNESP]Ayres, Jairo Aparecido [UNESP]Parise Fortes, Maria Rita [UNESP]Calvi, Sueli Aparecida [UNESP]2014-12-03T13:10:32Z2014-12-03T13:10:32Z2014-02-18info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article10application/pdfhttp://dx.doi.org/10.1371/journal.pone.0088572Plos One. San Francisco: Public Library Science, v. 9, n. 2, 10 p., 2014.1932-6203http://hdl.handle.net/11449/11222710.1371/journal.pone.0088572WOS:000331706700047WOS000331706700047.pdf84599811077897092179450022699059Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPLOS ONE2.7661,164info:eu-repo/semantics/openAccess2024-08-15T18:46:58Zoai:repositorio.unesp.br:11449/112227Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-15T18:46:58Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Analysis of Toll-Like Receptors, iNOS and Cytokine Profiles in Patients with Pulmonary Tuberculosis during Anti-Tuberculosis Treatment |
title |
Analysis of Toll-Like Receptors, iNOS and Cytokine Profiles in Patients with Pulmonary Tuberculosis during Anti-Tuberculosis Treatment |
spellingShingle |
Analysis of Toll-Like Receptors, iNOS and Cytokine Profiles in Patients with Pulmonary Tuberculosis during Anti-Tuberculosis Treatment Cardoso de Oliveira, Larissa Ragozo [UNESP] |
title_short |
Analysis of Toll-Like Receptors, iNOS and Cytokine Profiles in Patients with Pulmonary Tuberculosis during Anti-Tuberculosis Treatment |
title_full |
Analysis of Toll-Like Receptors, iNOS and Cytokine Profiles in Patients with Pulmonary Tuberculosis during Anti-Tuberculosis Treatment |
title_fullStr |
Analysis of Toll-Like Receptors, iNOS and Cytokine Profiles in Patients with Pulmonary Tuberculosis during Anti-Tuberculosis Treatment |
title_full_unstemmed |
Analysis of Toll-Like Receptors, iNOS and Cytokine Profiles in Patients with Pulmonary Tuberculosis during Anti-Tuberculosis Treatment |
title_sort |
Analysis of Toll-Like Receptors, iNOS and Cytokine Profiles in Patients with Pulmonary Tuberculosis during Anti-Tuberculosis Treatment |
author |
Cardoso de Oliveira, Larissa Ragozo [UNESP] |
author_facet |
Cardoso de Oliveira, Larissa Ragozo [UNESP] Peresi, Eliana [UNESP] Golim, Márjorie de Assis [UNESP] Gatto, Mariana [UNESP] Araujo Junior, Joao Pessoa [UNESP] Pellison Nunes da Costa, Erika Alessandra [UNESP] Ayres, Jairo Aparecido [UNESP] Parise Fortes, Maria Rita [UNESP] Calvi, Sueli Aparecida [UNESP] |
author_role |
author |
author2 |
Peresi, Eliana [UNESP] Golim, Márjorie de Assis [UNESP] Gatto, Mariana [UNESP] Araujo Junior, Joao Pessoa [UNESP] Pellison Nunes da Costa, Erika Alessandra [UNESP] Ayres, Jairo Aparecido [UNESP] Parise Fortes, Maria Rita [UNESP] Calvi, Sueli Aparecida [UNESP] |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Cardoso de Oliveira, Larissa Ragozo [UNESP] Peresi, Eliana [UNESP] Golim, Márjorie de Assis [UNESP] Gatto, Mariana [UNESP] Araujo Junior, Joao Pessoa [UNESP] Pellison Nunes da Costa, Erika Alessandra [UNESP] Ayres, Jairo Aparecido [UNESP] Parise Fortes, Maria Rita [UNESP] Calvi, Sueli Aparecida [UNESP] |
description |
Toll-like receptors (TLRs) play an important role in mycobacterial infection, although little is known about the roles of these receptors, cytokines and nitric oxide during anti-tuberculosis treatment. Our objective was to evaluate the mRNA and cell surface expression of TLR2 and TLR4; inducible nitric oxide synthase (iNOS) expression; and cytokine Th1, Th2 and Th17 profiles in pulmonary tuberculosis patients at different time points of anti-tuberculosis treatment. Peripheral blood mononuclear cells (PBMCs) were obtained from PPD+ healthy controls and from patients receiving anti-tuberculosis treatment. Gene expression quantification was performed by qPCR, cell surface expression was assessed using flow cytometry, and cytokine quantification was conducted using the CBA technique. The treated patients presented higher gene expression and higher numbers of receptors on the cell surface of lymphocytes and monocytes than did control individuals. IL-12 and IFN-gamma levels increased after the start of treatment, whereas TNF-alpha levels were reduced. TGF-beta presented the highest levels during treatment. IL-10 and IL-17 expression and production tended to increase during treatment. iNOS gene expression was reduced throughout treatment in patients. Our results suggest that anti-tuberculosis treatment modulates the immune response, inducing an increase in the expression of TLRs and pro-and anti-inflammatory cytokines to combat bacteria and reduce the inflammatory process. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-12-03T13:10:32Z 2014-12-03T13:10:32Z 2014-02-18 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1371/journal.pone.0088572 Plos One. San Francisco: Public Library Science, v. 9, n. 2, 10 p., 2014. 1932-6203 http://hdl.handle.net/11449/112227 10.1371/journal.pone.0088572 WOS:000331706700047 WOS000331706700047.pdf 8459981107789709 2179450022699059 |
url |
http://dx.doi.org/10.1371/journal.pone.0088572 http://hdl.handle.net/11449/112227 |
identifier_str_mv |
Plos One. San Francisco: Public Library Science, v. 9, n. 2, 10 p., 2014. 1932-6203 10.1371/journal.pone.0088572 WOS:000331706700047 WOS000331706700047.pdf 8459981107789709 2179450022699059 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
PLOS ONE 2.766 1,164 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
10 application/pdf |
dc.publisher.none.fl_str_mv |
Public Library Science |
publisher.none.fl_str_mv |
Public Library Science |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1808128160009551872 |