Proline Exogenously Supplied or Endogenously Overproduced Induces Different Nutritional, Metabolic, and Antioxidative Responses in Transgenic Tobacco Exposed to Cadmium
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s00344-021-10480-6 http://hdl.handle.net/11449/222346 |
Resumo: | Proline plays adaptive roles in plant tolerance to cadmium (Cd)-induced stress, but many gaps remain to be elucidated as the responses triggered by exogenously supplied proline or endogenously overproduction are not well known. Thus, we assayed the nutritional status, metabolite profiling, and antioxidative responses in wild type and transgenic tobacco (Nicotiana tabacum L.) containing the P5CSF129A gene under control of the cauliflower mosaic virus (CaMV35S) or stress inducible rd29A promoters. The plants were exposed or unexposed to Cd (0 and 50 μmol L−1 CdCl2·H2O) for 24 and 72 h. The wild type plants were also treated with or without exogenous proline (1 mmol L−1). Plants supplied with exogenous proline exhibited lower Cd translocation from roots to leaves than plants overproducing proline, avoiding oxidative damages in the leaves of these plants. Meanwhile, tobacco overproducing proline was less susceptible to Cd-induced nutritional changes than wild type plants and presented better metabolic adjustment under Cd exposure compared to plants supplied with exogenous proline. Plants overproducing proline increased the synthesis of sugars and organic acids under Cd exposure, which contributed to absence of oxidative stress, since both superoxide dismutase and catalase were not active against Cd-induced oxidative stress in these genotypes. Plants overproducing proline under the control of rd29A presented higher proline concentration in comparison to the CaMV35S promoter. With exception of rd29A plants that presented high proline and reduced glutathione (GSH) concentrations, the other plants presented an inverse correlation between proline and GSH synthesis after 72 h of Cd exposure. |
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Proline Exogenously Supplied or Endogenously Overproduced Induces Different Nutritional, Metabolic, and Antioxidative Responses in Transgenic Tobacco Exposed to CadmiumGlutathioneMetabolite profilingNicotiana tabacum LNutrient homeostasisOxidative stressP5CSF129AProline plays adaptive roles in plant tolerance to cadmium (Cd)-induced stress, but many gaps remain to be elucidated as the responses triggered by exogenously supplied proline or endogenously overproduction are not well known. Thus, we assayed the nutritional status, metabolite profiling, and antioxidative responses in wild type and transgenic tobacco (Nicotiana tabacum L.) containing the P5CSF129A gene under control of the cauliflower mosaic virus (CaMV35S) or stress inducible rd29A promoters. The plants were exposed or unexposed to Cd (0 and 50 μmol L−1 CdCl2·H2O) for 24 and 72 h. The wild type plants were also treated with or without exogenous proline (1 mmol L−1). Plants supplied with exogenous proline exhibited lower Cd translocation from roots to leaves than plants overproducing proline, avoiding oxidative damages in the leaves of these plants. Meanwhile, tobacco overproducing proline was less susceptible to Cd-induced nutritional changes than wild type plants and presented better metabolic adjustment under Cd exposure compared to plants supplied with exogenous proline. Plants overproducing proline increased the synthesis of sugars and organic acids under Cd exposure, which contributed to absence of oxidative stress, since both superoxide dismutase and catalase were not active against Cd-induced oxidative stress in these genotypes. Plants overproducing proline under the control of rd29A presented higher proline concentration in comparison to the CaMV35S promoter. With exception of rd29A plants that presented high proline and reduced glutathione (GSH) concentrations, the other plants presented an inverse correlation between proline and GSH synthesis after 72 h of Cd exposure.College of Agriculture Luiz de Queiroz University of São PauloCenter for Nuclear Energy in Agriculture University of São PauloCentre for Environmental Sciences Hasselt UniversityInstitute of Biosciences São Paulo State UniversityDepartment of Mathematics Federal University of Technology of ParanáFederal University of Technology of ParanáDepartment of Agronomy University of Oeste PaulistaLaboratório de Genética Bioquímica de Plantas Departamento de Genética Escola Superior de Agricultura “Luiz de Queiroz”, Avenida Pádua Dias, 11, Caixa Postal 9Institute of Biosciences São Paulo State UniversityUniversidade de São Paulo (USP)Hasselt UniversityUniversidade Estadual Paulista (UNESP)Federal University of Technology of ParanáUniversity of Oeste PaulistaEscola Superior de Agricultura “Luiz de Queiroz”Borgo, LucéliaRabêlo, Flávio Henrique SilveiraBudzinski, Ilara Gabriela Frasson [UNESP]Cataldi, Thaís RegianiRamires, Thiago GentilSchaker, Patricia Dayane CarvalhoRibas, Alessandra FerreiraLabate, Carlos AlbertoLavres, JoséCuypers, AnnAzevedo, Ricardo Antunes2022-04-28T19:44:09Z2022-04-28T19:44:09Z2021-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1007/s00344-021-10480-6Journal of Plant Growth Regulation.1435-81070721-7595http://hdl.handle.net/11449/22234610.1007/s00344-021-10480-62-s2.0-85114178570Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Plant Growth Regulationinfo:eu-repo/semantics/openAccess2022-04-28T19:44:09Zoai:repositorio.unesp.br:11449/222346Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T21:34:13.971247Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Proline Exogenously Supplied or Endogenously Overproduced Induces Different Nutritional, Metabolic, and Antioxidative Responses in Transgenic Tobacco Exposed to Cadmium |
title |
Proline Exogenously Supplied or Endogenously Overproduced Induces Different Nutritional, Metabolic, and Antioxidative Responses in Transgenic Tobacco Exposed to Cadmium |
spellingShingle |
Proline Exogenously Supplied or Endogenously Overproduced Induces Different Nutritional, Metabolic, and Antioxidative Responses in Transgenic Tobacco Exposed to Cadmium Borgo, Lucélia Glutathione Metabolite profiling Nicotiana tabacum L Nutrient homeostasis Oxidative stress P5CSF129A |
title_short |
Proline Exogenously Supplied or Endogenously Overproduced Induces Different Nutritional, Metabolic, and Antioxidative Responses in Transgenic Tobacco Exposed to Cadmium |
title_full |
Proline Exogenously Supplied or Endogenously Overproduced Induces Different Nutritional, Metabolic, and Antioxidative Responses in Transgenic Tobacco Exposed to Cadmium |
title_fullStr |
Proline Exogenously Supplied or Endogenously Overproduced Induces Different Nutritional, Metabolic, and Antioxidative Responses in Transgenic Tobacco Exposed to Cadmium |
title_full_unstemmed |
Proline Exogenously Supplied or Endogenously Overproduced Induces Different Nutritional, Metabolic, and Antioxidative Responses in Transgenic Tobacco Exposed to Cadmium |
title_sort |
Proline Exogenously Supplied or Endogenously Overproduced Induces Different Nutritional, Metabolic, and Antioxidative Responses in Transgenic Tobacco Exposed to Cadmium |
author |
Borgo, Lucélia |
author_facet |
Borgo, Lucélia Rabêlo, Flávio Henrique Silveira Budzinski, Ilara Gabriela Frasson [UNESP] Cataldi, Thaís Regiani Ramires, Thiago Gentil Schaker, Patricia Dayane Carvalho Ribas, Alessandra Ferreira Labate, Carlos Alberto Lavres, José Cuypers, Ann Azevedo, Ricardo Antunes |
author_role |
author |
author2 |
Rabêlo, Flávio Henrique Silveira Budzinski, Ilara Gabriela Frasson [UNESP] Cataldi, Thaís Regiani Ramires, Thiago Gentil Schaker, Patricia Dayane Carvalho Ribas, Alessandra Ferreira Labate, Carlos Alberto Lavres, José Cuypers, Ann Azevedo, Ricardo Antunes |
author2_role |
author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) Hasselt University Universidade Estadual Paulista (UNESP) Federal University of Technology of Paraná University of Oeste Paulista Escola Superior de Agricultura “Luiz de Queiroz” |
dc.contributor.author.fl_str_mv |
Borgo, Lucélia Rabêlo, Flávio Henrique Silveira Budzinski, Ilara Gabriela Frasson [UNESP] Cataldi, Thaís Regiani Ramires, Thiago Gentil Schaker, Patricia Dayane Carvalho Ribas, Alessandra Ferreira Labate, Carlos Alberto Lavres, José Cuypers, Ann Azevedo, Ricardo Antunes |
dc.subject.por.fl_str_mv |
Glutathione Metabolite profiling Nicotiana tabacum L Nutrient homeostasis Oxidative stress P5CSF129A |
topic |
Glutathione Metabolite profiling Nicotiana tabacum L Nutrient homeostasis Oxidative stress P5CSF129A |
description |
Proline plays adaptive roles in plant tolerance to cadmium (Cd)-induced stress, but many gaps remain to be elucidated as the responses triggered by exogenously supplied proline or endogenously overproduction are not well known. Thus, we assayed the nutritional status, metabolite profiling, and antioxidative responses in wild type and transgenic tobacco (Nicotiana tabacum L.) containing the P5CSF129A gene under control of the cauliflower mosaic virus (CaMV35S) or stress inducible rd29A promoters. The plants were exposed or unexposed to Cd (0 and 50 μmol L−1 CdCl2·H2O) for 24 and 72 h. The wild type plants were also treated with or without exogenous proline (1 mmol L−1). Plants supplied with exogenous proline exhibited lower Cd translocation from roots to leaves than plants overproducing proline, avoiding oxidative damages in the leaves of these plants. Meanwhile, tobacco overproducing proline was less susceptible to Cd-induced nutritional changes than wild type plants and presented better metabolic adjustment under Cd exposure compared to plants supplied with exogenous proline. Plants overproducing proline increased the synthesis of sugars and organic acids under Cd exposure, which contributed to absence of oxidative stress, since both superoxide dismutase and catalase were not active against Cd-induced oxidative stress in these genotypes. Plants overproducing proline under the control of rd29A presented higher proline concentration in comparison to the CaMV35S promoter. With exception of rd29A plants that presented high proline and reduced glutathione (GSH) concentrations, the other plants presented an inverse correlation between proline and GSH synthesis after 72 h of Cd exposure. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-01-01 2022-04-28T19:44:09Z 2022-04-28T19:44:09Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s00344-021-10480-6 Journal of Plant Growth Regulation. 1435-8107 0721-7595 http://hdl.handle.net/11449/222346 10.1007/s00344-021-10480-6 2-s2.0-85114178570 |
url |
http://dx.doi.org/10.1007/s00344-021-10480-6 http://hdl.handle.net/11449/222346 |
identifier_str_mv |
Journal of Plant Growth Regulation. 1435-8107 0721-7595 10.1007/s00344-021-10480-6 2-s2.0-85114178570 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Plant Growth Regulation |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129336949080064 |