Proline Exogenously Supplied or Endogenously Overproduced Induces Different Nutritional, Metabolic, and Antioxidative Responses in Transgenic Tobacco Exposed to Cadmium

Detalhes bibliográficos
Autor(a) principal: Borgo, Lucélia
Data de Publicação: 2021
Outros Autores: Rabêlo, Flávio Henrique Silveira, Budzinski, Ilara Gabriela Frasson [UNESP], Cataldi, Thaís Regiani, Ramires, Thiago Gentil, Schaker, Patricia Dayane Carvalho, Ribas, Alessandra Ferreira, Labate, Carlos Alberto, Lavres, José, Cuypers, Ann, Azevedo, Ricardo Antunes
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1007/s00344-021-10480-6
http://hdl.handle.net/11449/222346
Resumo: Proline plays adaptive roles in plant tolerance to cadmium (Cd)-induced stress, but many gaps remain to be elucidated as the responses triggered by exogenously supplied proline or endogenously overproduction are not well known. Thus, we assayed the nutritional status, metabolite profiling, and antioxidative responses in wild type and transgenic tobacco (Nicotiana tabacum L.) containing the P5CSF129A gene under control of the cauliflower mosaic virus (CaMV35S) or stress inducible rd29A promoters. The plants were exposed or unexposed to Cd (0 and 50 μmol L−1 CdCl2·H2O) for 24 and 72 h. The wild type plants were also treated with or without exogenous proline (1 mmol L−1). Plants supplied with exogenous proline exhibited lower Cd translocation from roots to leaves than plants overproducing proline, avoiding oxidative damages in the leaves of these plants. Meanwhile, tobacco overproducing proline was less susceptible to Cd-induced nutritional changes than wild type plants and presented better metabolic adjustment under Cd exposure compared to plants supplied with exogenous proline. Plants overproducing proline increased the synthesis of sugars and organic acids under Cd exposure, which contributed to absence of oxidative stress, since both superoxide dismutase and catalase were not active against Cd-induced oxidative stress in these genotypes. Plants overproducing proline under the control of rd29A presented higher proline concentration in comparison to the CaMV35S promoter. With exception of rd29A plants that presented high proline and reduced glutathione (GSH) concentrations, the other plants presented an inverse correlation between proline and GSH synthesis after 72 h of Cd exposure.
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spelling Proline Exogenously Supplied or Endogenously Overproduced Induces Different Nutritional, Metabolic, and Antioxidative Responses in Transgenic Tobacco Exposed to CadmiumGlutathioneMetabolite profilingNicotiana tabacum LNutrient homeostasisOxidative stressP5CSF129AProline plays adaptive roles in plant tolerance to cadmium (Cd)-induced stress, but many gaps remain to be elucidated as the responses triggered by exogenously supplied proline or endogenously overproduction are not well known. Thus, we assayed the nutritional status, metabolite profiling, and antioxidative responses in wild type and transgenic tobacco (Nicotiana tabacum L.) containing the P5CSF129A gene under control of the cauliflower mosaic virus (CaMV35S) or stress inducible rd29A promoters. The plants were exposed or unexposed to Cd (0 and 50 μmol L−1 CdCl2·H2O) for 24 and 72 h. The wild type plants were also treated with or without exogenous proline (1 mmol L−1). Plants supplied with exogenous proline exhibited lower Cd translocation from roots to leaves than plants overproducing proline, avoiding oxidative damages in the leaves of these plants. Meanwhile, tobacco overproducing proline was less susceptible to Cd-induced nutritional changes than wild type plants and presented better metabolic adjustment under Cd exposure compared to plants supplied with exogenous proline. Plants overproducing proline increased the synthesis of sugars and organic acids under Cd exposure, which contributed to absence of oxidative stress, since both superoxide dismutase and catalase were not active against Cd-induced oxidative stress in these genotypes. Plants overproducing proline under the control of rd29A presented higher proline concentration in comparison to the CaMV35S promoter. With exception of rd29A plants that presented high proline and reduced glutathione (GSH) concentrations, the other plants presented an inverse correlation between proline and GSH synthesis after 72 h of Cd exposure.College of Agriculture Luiz de Queiroz University of São PauloCenter for Nuclear Energy in Agriculture University of São PauloCentre for Environmental Sciences Hasselt UniversityInstitute of Biosciences São Paulo State UniversityDepartment of Mathematics Federal University of Technology of ParanáFederal University of Technology of ParanáDepartment of Agronomy University of Oeste PaulistaLaboratório de Genética Bioquímica de Plantas Departamento de Genética Escola Superior de Agricultura “Luiz de Queiroz”, Avenida Pádua Dias, 11, Caixa Postal 9Institute of Biosciences São Paulo State UniversityUniversidade de São Paulo (USP)Hasselt UniversityUniversidade Estadual Paulista (UNESP)Federal University of Technology of ParanáUniversity of Oeste PaulistaEscola Superior de Agricultura “Luiz de Queiroz”Borgo, LucéliaRabêlo, Flávio Henrique SilveiraBudzinski, Ilara Gabriela Frasson [UNESP]Cataldi, Thaís RegianiRamires, Thiago GentilSchaker, Patricia Dayane CarvalhoRibas, Alessandra FerreiraLabate, Carlos AlbertoLavres, JoséCuypers, AnnAzevedo, Ricardo Antunes2022-04-28T19:44:09Z2022-04-28T19:44:09Z2021-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1007/s00344-021-10480-6Journal of Plant Growth Regulation.1435-81070721-7595http://hdl.handle.net/11449/22234610.1007/s00344-021-10480-62-s2.0-85114178570Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Plant Growth Regulationinfo:eu-repo/semantics/openAccess2022-04-28T19:44:09Zoai:repositorio.unesp.br:11449/222346Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T21:34:13.971247Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Proline Exogenously Supplied or Endogenously Overproduced Induces Different Nutritional, Metabolic, and Antioxidative Responses in Transgenic Tobacco Exposed to Cadmium
title Proline Exogenously Supplied or Endogenously Overproduced Induces Different Nutritional, Metabolic, and Antioxidative Responses in Transgenic Tobacco Exposed to Cadmium
spellingShingle Proline Exogenously Supplied or Endogenously Overproduced Induces Different Nutritional, Metabolic, and Antioxidative Responses in Transgenic Tobacco Exposed to Cadmium
Borgo, Lucélia
Glutathione
Metabolite profiling
Nicotiana tabacum L
Nutrient homeostasis
Oxidative stress
P5CSF129A
title_short Proline Exogenously Supplied or Endogenously Overproduced Induces Different Nutritional, Metabolic, and Antioxidative Responses in Transgenic Tobacco Exposed to Cadmium
title_full Proline Exogenously Supplied or Endogenously Overproduced Induces Different Nutritional, Metabolic, and Antioxidative Responses in Transgenic Tobacco Exposed to Cadmium
title_fullStr Proline Exogenously Supplied or Endogenously Overproduced Induces Different Nutritional, Metabolic, and Antioxidative Responses in Transgenic Tobacco Exposed to Cadmium
title_full_unstemmed Proline Exogenously Supplied or Endogenously Overproduced Induces Different Nutritional, Metabolic, and Antioxidative Responses in Transgenic Tobacco Exposed to Cadmium
title_sort Proline Exogenously Supplied or Endogenously Overproduced Induces Different Nutritional, Metabolic, and Antioxidative Responses in Transgenic Tobacco Exposed to Cadmium
author Borgo, Lucélia
author_facet Borgo, Lucélia
Rabêlo, Flávio Henrique Silveira
Budzinski, Ilara Gabriela Frasson [UNESP]
Cataldi, Thaís Regiani
Ramires, Thiago Gentil
Schaker, Patricia Dayane Carvalho
Ribas, Alessandra Ferreira
Labate, Carlos Alberto
Lavres, José
Cuypers, Ann
Azevedo, Ricardo Antunes
author_role author
author2 Rabêlo, Flávio Henrique Silveira
Budzinski, Ilara Gabriela Frasson [UNESP]
Cataldi, Thaís Regiani
Ramires, Thiago Gentil
Schaker, Patricia Dayane Carvalho
Ribas, Alessandra Ferreira
Labate, Carlos Alberto
Lavres, José
Cuypers, Ann
Azevedo, Ricardo Antunes
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade de São Paulo (USP)
Hasselt University
Universidade Estadual Paulista (UNESP)
Federal University of Technology of Paraná
University of Oeste Paulista
Escola Superior de Agricultura “Luiz de Queiroz”
dc.contributor.author.fl_str_mv Borgo, Lucélia
Rabêlo, Flávio Henrique Silveira
Budzinski, Ilara Gabriela Frasson [UNESP]
Cataldi, Thaís Regiani
Ramires, Thiago Gentil
Schaker, Patricia Dayane Carvalho
Ribas, Alessandra Ferreira
Labate, Carlos Alberto
Lavres, José
Cuypers, Ann
Azevedo, Ricardo Antunes
dc.subject.por.fl_str_mv Glutathione
Metabolite profiling
Nicotiana tabacum L
Nutrient homeostasis
Oxidative stress
P5CSF129A
topic Glutathione
Metabolite profiling
Nicotiana tabacum L
Nutrient homeostasis
Oxidative stress
P5CSF129A
description Proline plays adaptive roles in plant tolerance to cadmium (Cd)-induced stress, but many gaps remain to be elucidated as the responses triggered by exogenously supplied proline or endogenously overproduction are not well known. Thus, we assayed the nutritional status, metabolite profiling, and antioxidative responses in wild type and transgenic tobacco (Nicotiana tabacum L.) containing the P5CSF129A gene under control of the cauliflower mosaic virus (CaMV35S) or stress inducible rd29A promoters. The plants were exposed or unexposed to Cd (0 and 50 μmol L−1 CdCl2·H2O) for 24 and 72 h. The wild type plants were also treated with or without exogenous proline (1 mmol L−1). Plants supplied with exogenous proline exhibited lower Cd translocation from roots to leaves than plants overproducing proline, avoiding oxidative damages in the leaves of these plants. Meanwhile, tobacco overproducing proline was less susceptible to Cd-induced nutritional changes than wild type plants and presented better metabolic adjustment under Cd exposure compared to plants supplied with exogenous proline. Plants overproducing proline increased the synthesis of sugars and organic acids under Cd exposure, which contributed to absence of oxidative stress, since both superoxide dismutase and catalase were not active against Cd-induced oxidative stress in these genotypes. Plants overproducing proline under the control of rd29A presented higher proline concentration in comparison to the CaMV35S promoter. With exception of rd29A plants that presented high proline and reduced glutathione (GSH) concentrations, the other plants presented an inverse correlation between proline and GSH synthesis after 72 h of Cd exposure.
publishDate 2021
dc.date.none.fl_str_mv 2021-01-01
2022-04-28T19:44:09Z
2022-04-28T19:44:09Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1007/s00344-021-10480-6
Journal of Plant Growth Regulation.
1435-8107
0721-7595
http://hdl.handle.net/11449/222346
10.1007/s00344-021-10480-6
2-s2.0-85114178570
url http://dx.doi.org/10.1007/s00344-021-10480-6
http://hdl.handle.net/11449/222346
identifier_str_mv Journal of Plant Growth Regulation.
1435-8107
0721-7595
10.1007/s00344-021-10480-6
2-s2.0-85114178570
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Journal of Plant Growth Regulation
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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