Carbonic anhydrase activation enhances object recognition memory in mice through phosphorylation of the extracellular signal-regulated kinase in the cortex and the hippocampus
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.neuropharm.2017.03.009 http://hdl.handle.net/11449/169570 |
Resumo: | Rats injected with by D-phenylalanine, a carbonic anhydrase (CA) activator, enhanced spatial learning, whereas rats given acetazolamide, a CA inhibitor, exhibited impairments of fear memory consolidation. However, the related mechanisms are unclear. We investigated if CAs are involved in a non-spatial recognition memory task assessed using the object recognition test (ORT). Systemic administration of acetazolamide to male CD1 mice caused amnesia in the ORT and reduced CA activity in brain homogenates, while treatment with D-phenylalanine enhanced memory and increased CA activity. We provided also the first evidence that D-phenylalanine administration rapidly activated extracellular signal-regulated kinase (ERK) pathways, a critical step for memory formation, in the cortex and the hippocampus, two brain areas involved in memory processing. Effects elicited by D-phenylalanine were completely blunted by co-administration of acetazolamide, but not of 1-N-(4-sulfamoylphenyl-ethyl)-2,4,6-trimethylpyridinium perchlorate (C18), a CA inhibitor that, differently from acetazolamide, does not cross the blood brain barrier. Our results strongly suggest that brain but not peripheral CAs activation potentiates memory as a result of ERK pathway enhanced activation. |
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Carbonic anhydrase activation enhances object recognition memory in mice through phosphorylation of the extracellular signal-regulated kinase in the cortex and the hippocampus1-N-(4-sulfamoylphenyl-ethyl)-2,4,6-Trimethylpyridinium perchlorateAcetazolamideD-phenylalanineRats injected with by D-phenylalanine, a carbonic anhydrase (CA) activator, enhanced spatial learning, whereas rats given acetazolamide, a CA inhibitor, exhibited impairments of fear memory consolidation. However, the related mechanisms are unclear. We investigated if CAs are involved in a non-spatial recognition memory task assessed using the object recognition test (ORT). Systemic administration of acetazolamide to male CD1 mice caused amnesia in the ORT and reduced CA activity in brain homogenates, while treatment with D-phenylalanine enhanced memory and increased CA activity. We provided also the first evidence that D-phenylalanine administration rapidly activated extracellular signal-regulated kinase (ERK) pathways, a critical step for memory formation, in the cortex and the hippocampus, two brain areas involved in memory processing. Effects elicited by D-phenylalanine were completely blunted by co-administration of acetazolamide, but not of 1-N-(4-sulfamoylphenyl-ethyl)-2,4,6-trimethylpyridinium perchlorate (C18), a CA inhibitor that, differently from acetazolamide, does not cross the blood brain barrier. Our results strongly suggest that brain but not peripheral CAs activation potentiates memory as a result of ERK pathway enhanced activation.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fondazione Umberto VeronesiDipartimento di Neuroscienze Psicologia Area del Farmaco e Salute del Bambino Università degli Studi di FirenzeDipartimento di Chimica 'Ugo Schiff' Università degli Studi di FirenzeDipartimento di Scienze della Salute Università degli Studi di FirenzeFaculdade de Ciências Farmacêuticas Universidade Estadual Paulista-UNESPFaculdade de Ciências Farmacêuticas Universidade Estadual Paulista-UNESPCNPq: 201511/2014-2Università degli Studi di FirenzeUniversidade Estadual Paulista (Unesp)Canto de Souza, Lucas [UNESP]Provensi, GustavoVullo, DanielaCarta, FabrizioScozzafava, AndreaCosta, AlessiaSchmidt, Scheila DaianePassani, Maria BeatriceSupuran, Claudiu T.Blandina, Patrizio2018-12-11T16:46:29Z2018-12-11T16:46:29Z2017-05-15info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article148-156application/pdfhttp://dx.doi.org/10.1016/j.neuropharm.2017.03.009Neuropharmacology, v. 118, p. 148-156.1873-70640028-3908http://hdl.handle.net/11449/16957010.1016/j.neuropharm.2017.03.0092-s2.0-850160872232-s2.0-85016087223.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengNeuropharmacology2,043info:eu-repo/semantics/openAccess2024-01-28T06:48:35Zoai:repositorio.unesp.br:11449/169570Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-06T00:08:45.906712Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Carbonic anhydrase activation enhances object recognition memory in mice through phosphorylation of the extracellular signal-regulated kinase in the cortex and the hippocampus |
title |
Carbonic anhydrase activation enhances object recognition memory in mice through phosphorylation of the extracellular signal-regulated kinase in the cortex and the hippocampus |
spellingShingle |
Carbonic anhydrase activation enhances object recognition memory in mice through phosphorylation of the extracellular signal-regulated kinase in the cortex and the hippocampus Canto de Souza, Lucas [UNESP] 1-N-(4-sulfamoylphenyl-ethyl)-2,4,6-Trimethylpyridinium perchlorate Acetazolamide D-phenylalanine |
title_short |
Carbonic anhydrase activation enhances object recognition memory in mice through phosphorylation of the extracellular signal-regulated kinase in the cortex and the hippocampus |
title_full |
Carbonic anhydrase activation enhances object recognition memory in mice through phosphorylation of the extracellular signal-regulated kinase in the cortex and the hippocampus |
title_fullStr |
Carbonic anhydrase activation enhances object recognition memory in mice through phosphorylation of the extracellular signal-regulated kinase in the cortex and the hippocampus |
title_full_unstemmed |
Carbonic anhydrase activation enhances object recognition memory in mice through phosphorylation of the extracellular signal-regulated kinase in the cortex and the hippocampus |
title_sort |
Carbonic anhydrase activation enhances object recognition memory in mice through phosphorylation of the extracellular signal-regulated kinase in the cortex and the hippocampus |
author |
Canto de Souza, Lucas [UNESP] |
author_facet |
Canto de Souza, Lucas [UNESP] Provensi, Gustavo Vullo, Daniela Carta, Fabrizio Scozzafava, Andrea Costa, Alessia Schmidt, Scheila Daiane Passani, Maria Beatrice Supuran, Claudiu T. Blandina, Patrizio |
author_role |
author |
author2 |
Provensi, Gustavo Vullo, Daniela Carta, Fabrizio Scozzafava, Andrea Costa, Alessia Schmidt, Scheila Daiane Passani, Maria Beatrice Supuran, Claudiu T. Blandina, Patrizio |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Università degli Studi di Firenze Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Canto de Souza, Lucas [UNESP] Provensi, Gustavo Vullo, Daniela Carta, Fabrizio Scozzafava, Andrea Costa, Alessia Schmidt, Scheila Daiane Passani, Maria Beatrice Supuran, Claudiu T. Blandina, Patrizio |
dc.subject.por.fl_str_mv |
1-N-(4-sulfamoylphenyl-ethyl)-2,4,6-Trimethylpyridinium perchlorate Acetazolamide D-phenylalanine |
topic |
1-N-(4-sulfamoylphenyl-ethyl)-2,4,6-Trimethylpyridinium perchlorate Acetazolamide D-phenylalanine |
description |
Rats injected with by D-phenylalanine, a carbonic anhydrase (CA) activator, enhanced spatial learning, whereas rats given acetazolamide, a CA inhibitor, exhibited impairments of fear memory consolidation. However, the related mechanisms are unclear. We investigated if CAs are involved in a non-spatial recognition memory task assessed using the object recognition test (ORT). Systemic administration of acetazolamide to male CD1 mice caused amnesia in the ORT and reduced CA activity in brain homogenates, while treatment with D-phenylalanine enhanced memory and increased CA activity. We provided also the first evidence that D-phenylalanine administration rapidly activated extracellular signal-regulated kinase (ERK) pathways, a critical step for memory formation, in the cortex and the hippocampus, two brain areas involved in memory processing. Effects elicited by D-phenylalanine were completely blunted by co-administration of acetazolamide, but not of 1-N-(4-sulfamoylphenyl-ethyl)-2,4,6-trimethylpyridinium perchlorate (C18), a CA inhibitor that, differently from acetazolamide, does not cross the blood brain barrier. Our results strongly suggest that brain but not peripheral CAs activation potentiates memory as a result of ERK pathway enhanced activation. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-05-15 2018-12-11T16:46:29Z 2018-12-11T16:46:29Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.neuropharm.2017.03.009 Neuropharmacology, v. 118, p. 148-156. 1873-7064 0028-3908 http://hdl.handle.net/11449/169570 10.1016/j.neuropharm.2017.03.009 2-s2.0-85016087223 2-s2.0-85016087223.pdf |
url |
http://dx.doi.org/10.1016/j.neuropharm.2017.03.009 http://hdl.handle.net/11449/169570 |
identifier_str_mv |
Neuropharmacology, v. 118, p. 148-156. 1873-7064 0028-3908 10.1016/j.neuropharm.2017.03.009 2-s2.0-85016087223 2-s2.0-85016087223.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Neuropharmacology 2,043 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
148-156 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129589274214400 |