Quantificação do interferon-tau durante o reconhecimento materno da gestação em fêmeas bovinas Bos taurus indicus

Detalhes bibliográficos
Autor(a) principal: Giassetti, Mariana Ianello
Data de Publicação: 2008
Outros Autores: Pontes, Eduardo Oliveira, Niemeyer, Claudia, Siqueira, Adriano Felipe Perez, Fedozzi, Filipe, Lima, Marcelo Cardoso, Marques, Vanessa Belentani, Bertan, Claudia Maria [UNESP], Miglino, Maria Angélica, Arruda, Rubens Paes, Papa, Paula Carvalho, Binelli, Mario
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://www.revistas.usp.br/bjvras/article/view/26738
http://hdl.handle.net/11449/70819
Resumo: During the critical period of the maternal recognition, which occurs between days 15 and 19 of pregnancy, the conceptus must competently synthesize molecules capable of blocking the synthesis of prostaglandin F2α (PGF2α) and luteolysis. In cattle, the major macromolecule involved in suck blockage is the protein interferontau (IFN-τ). During the critical period, failures in the recognition of pregnancy determine embryonic mortality on up to 40% of inseminated cows. Data about IFN-τ in Bos taurus indicus are still scarce. Objective of this study was to quantitatively evaluate the presence of IFN-τ during the critical period for maternal recognition of pregnancy in uterine flushings obtained in vivo by Foley catheter (Days 14, 16 and 18 post estrus) or post-mortem (Day 18 post estrus). Multiparous, cyclic or pregnant zebu cows (Bos taurus indicus) on days 14, 16 and 18 post estrus were used for in vivo or post mortem uterine flushing collection. In both cases, a Ringer solution was used to wash the uterus of cows. Uterine flushings were concentrated by ultrafiltration and lyophilized. Proteins were separated by one-dimensional electrophoresis (SDS-PAGE) in a 15% polyacrilamide gel. Interferontau quantification in uterine flushings was performed by western blotting and densitometry. Non-specific protein bands were observed in both in vivo and post mortem uterine flushings. Interferon-tau was detected only in uterine flushings obtained from pregnant cows post-mortem (P<0.05). Optical density of protein bands was not affected by the day of the critical period, state (cyclic or pregnant) or interaction day x state. There was no effect of the conceptus weight or progesterone concentration on the day of uterine flushing collection in the optical density of the IFN-τ protein band. It was concluded that the detection and quantification of IFN-τ in the uterine environment of zebu cows, in these experimental?conditions, is only possible in uterine flushings obtained post-mortem.
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spelling Quantificação do interferon-tau durante o reconhecimento materno da gestação em fêmeas bovinas Bos taurus indicusQuantification of interferon-tau during the maternal recognition of pregnancy in Bos taurus indicus cowsBovineIFN-τPregnancyUterine environmentWestern-blottingBosBos indicusBovinaeDuring the critical period of the maternal recognition, which occurs between days 15 and 19 of pregnancy, the conceptus must competently synthesize molecules capable of blocking the synthesis of prostaglandin F2α (PGF2α) and luteolysis. In cattle, the major macromolecule involved in suck blockage is the protein interferontau (IFN-τ). During the critical period, failures in the recognition of pregnancy determine embryonic mortality on up to 40% of inseminated cows. Data about IFN-τ in Bos taurus indicus are still scarce. Objective of this study was to quantitatively evaluate the presence of IFN-τ during the critical period for maternal recognition of pregnancy in uterine flushings obtained in vivo by Foley catheter (Days 14, 16 and 18 post estrus) or post-mortem (Day 18 post estrus). Multiparous, cyclic or pregnant zebu cows (Bos taurus indicus) on days 14, 16 and 18 post estrus were used for in vivo or post mortem uterine flushing collection. In both cases, a Ringer solution was used to wash the uterus of cows. Uterine flushings were concentrated by ultrafiltration and lyophilized. Proteins were separated by one-dimensional electrophoresis (SDS-PAGE) in a 15% polyacrilamide gel. Interferontau quantification in uterine flushings was performed by western blotting and densitometry. Non-specific protein bands were observed in both in vivo and post mortem uterine flushings. Interferon-tau was detected only in uterine flushings obtained from pregnant cows post-mortem (P<0.05). Optical density of protein bands was not affected by the day of the critical period, state (cyclic or pregnant) or interaction day x state. There was no effect of the conceptus weight or progesterone concentration on the day of uterine flushing collection in the optical density of the IFN-τ protein band. It was concluded that the detection and quantification of IFN-τ in the uterine environment of zebu cows, in these experimental?conditions, is only possible in uterine flushings obtained post-mortem.Laboratório de Fisiologia e Endocrinologia Molecular Departamento de Reprodução Animal Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo-SPDepartamento de Cirurgia Faculdade de Medicina Veterinária e Zootecnia Universidade de São Paulo, São Paulo-SPLaboratório de Morfofisiologia da Placenta e do Embrião Faculdade de Zootecnia Universidade Estadual Paulista, Dracena-SPLaboratório de Morfofisiologia da Placenta e do Embrião Faculdade de Zootecnia Universidade Estadual Paulista, Dracena-SPUniversidade de São Paulo (USP)Universidade Estadual Paulista (Unesp)Giassetti, Mariana IanelloPontes, Eduardo OliveiraNiemeyer, ClaudiaSiqueira, Adriano Felipe PerezFedozzi, FilipeLima, Marcelo CardosoMarques, Vanessa BelentaniBertan, Claudia Maria [UNESP]Miglino, Maria AngélicaArruda, Rubens PaesPapa, Paula CarvalhoBinelli, Mario2014-05-27T11:23:48Z2014-05-27T11:23:48Z2008-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article111-120application/pdfhttp://www.revistas.usp.br/bjvras/article/view/26738Brazilian Journal of Veterinary Research and Animal Science, v. 45, n. SUPPL., p. 111-120, 2008.1413-95961678-4456http://hdl.handle.net/11449/708192-s2.0-781493983522-s2.0-78149398352.pdf57201624933978510000-0003-4211-0649Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPporBrazilian Journal of Veterinary Research and Animal Science0,2250,225info:eu-repo/semantics/openAccess2024-05-07T13:48:06Zoai:repositorio.unesp.br:11449/70819Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-06T00:00:02.318024Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Quantificação do interferon-tau durante o reconhecimento materno da gestação em fêmeas bovinas Bos taurus indicus
Quantification of interferon-tau during the maternal recognition of pregnancy in Bos taurus indicus cows
title Quantificação do interferon-tau durante o reconhecimento materno da gestação em fêmeas bovinas Bos taurus indicus
spellingShingle Quantificação do interferon-tau durante o reconhecimento materno da gestação em fêmeas bovinas Bos taurus indicus
Giassetti, Mariana Ianello
Bovine
IFN-τ
Pregnancy
Uterine environment
Western-blotting
Bos
Bos indicus
Bovinae
title_short Quantificação do interferon-tau durante o reconhecimento materno da gestação em fêmeas bovinas Bos taurus indicus
title_full Quantificação do interferon-tau durante o reconhecimento materno da gestação em fêmeas bovinas Bos taurus indicus
title_fullStr Quantificação do interferon-tau durante o reconhecimento materno da gestação em fêmeas bovinas Bos taurus indicus
title_full_unstemmed Quantificação do interferon-tau durante o reconhecimento materno da gestação em fêmeas bovinas Bos taurus indicus
title_sort Quantificação do interferon-tau durante o reconhecimento materno da gestação em fêmeas bovinas Bos taurus indicus
author Giassetti, Mariana Ianello
author_facet Giassetti, Mariana Ianello
Pontes, Eduardo Oliveira
Niemeyer, Claudia
Siqueira, Adriano Felipe Perez
Fedozzi, Filipe
Lima, Marcelo Cardoso
Marques, Vanessa Belentani
Bertan, Claudia Maria [UNESP]
Miglino, Maria Angélica
Arruda, Rubens Paes
Papa, Paula Carvalho
Binelli, Mario
author_role author
author2 Pontes, Eduardo Oliveira
Niemeyer, Claudia
Siqueira, Adriano Felipe Perez
Fedozzi, Filipe
Lima, Marcelo Cardoso
Marques, Vanessa Belentani
Bertan, Claudia Maria [UNESP]
Miglino, Maria Angélica
Arruda, Rubens Paes
Papa, Paula Carvalho
Binelli, Mario
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade de São Paulo (USP)
Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Giassetti, Mariana Ianello
Pontes, Eduardo Oliveira
Niemeyer, Claudia
Siqueira, Adriano Felipe Perez
Fedozzi, Filipe
Lima, Marcelo Cardoso
Marques, Vanessa Belentani
Bertan, Claudia Maria [UNESP]
Miglino, Maria Angélica
Arruda, Rubens Paes
Papa, Paula Carvalho
Binelli, Mario
dc.subject.por.fl_str_mv Bovine
IFN-τ
Pregnancy
Uterine environment
Western-blotting
Bos
Bos indicus
Bovinae
topic Bovine
IFN-τ
Pregnancy
Uterine environment
Western-blotting
Bos
Bos indicus
Bovinae
description During the critical period of the maternal recognition, which occurs between days 15 and 19 of pregnancy, the conceptus must competently synthesize molecules capable of blocking the synthesis of prostaglandin F2α (PGF2α) and luteolysis. In cattle, the major macromolecule involved in suck blockage is the protein interferontau (IFN-τ). During the critical period, failures in the recognition of pregnancy determine embryonic mortality on up to 40% of inseminated cows. Data about IFN-τ in Bos taurus indicus are still scarce. Objective of this study was to quantitatively evaluate the presence of IFN-τ during the critical period for maternal recognition of pregnancy in uterine flushings obtained in vivo by Foley catheter (Days 14, 16 and 18 post estrus) or post-mortem (Day 18 post estrus). Multiparous, cyclic or pregnant zebu cows (Bos taurus indicus) on days 14, 16 and 18 post estrus were used for in vivo or post mortem uterine flushing collection. In both cases, a Ringer solution was used to wash the uterus of cows. Uterine flushings were concentrated by ultrafiltration and lyophilized. Proteins were separated by one-dimensional electrophoresis (SDS-PAGE) in a 15% polyacrilamide gel. Interferontau quantification in uterine flushings was performed by western blotting and densitometry. Non-specific protein bands were observed in both in vivo and post mortem uterine flushings. Interferon-tau was detected only in uterine flushings obtained from pregnant cows post-mortem (P<0.05). Optical density of protein bands was not affected by the day of the critical period, state (cyclic or pregnant) or interaction day x state. There was no effect of the conceptus weight or progesterone concentration on the day of uterine flushing collection in the optical density of the IFN-τ protein band. It was concluded that the detection and quantification of IFN-τ in the uterine environment of zebu cows, in these experimental?conditions, is only possible in uterine flushings obtained post-mortem.
publishDate 2008
dc.date.none.fl_str_mv 2008-12-01
2014-05-27T11:23:48Z
2014-05-27T11:23:48Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.revistas.usp.br/bjvras/article/view/26738
Brazilian Journal of Veterinary Research and Animal Science, v. 45, n. SUPPL., p. 111-120, 2008.
1413-9596
1678-4456
http://hdl.handle.net/11449/70819
2-s2.0-78149398352
2-s2.0-78149398352.pdf
5720162493397851
0000-0003-4211-0649
url http://www.revistas.usp.br/bjvras/article/view/26738
http://hdl.handle.net/11449/70819
identifier_str_mv Brazilian Journal of Veterinary Research and Animal Science, v. 45, n. SUPPL., p. 111-120, 2008.
1413-9596
1678-4456
2-s2.0-78149398352
2-s2.0-78149398352.pdf
5720162493397851
0000-0003-4211-0649
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv Brazilian Journal of Veterinary Research and Animal Science
0,225
0,225
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 111-120
application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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