Quantificação do interferon-tau durante o reconhecimento materno da gestação em fêmeas bovinas Bos taurus indicus
Autor(a) principal: | |
---|---|
Data de Publicação: | 2008 |
Outros Autores: | , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://www.revistas.usp.br/bjvras/article/view/26738 http://hdl.handle.net/11449/70819 |
Resumo: | During the critical period of the maternal recognition, which occurs between days 15 and 19 of pregnancy, the conceptus must competently synthesize molecules capable of blocking the synthesis of prostaglandin F2α (PGF2α) and luteolysis. In cattle, the major macromolecule involved in suck blockage is the protein interferontau (IFN-τ). During the critical period, failures in the recognition of pregnancy determine embryonic mortality on up to 40% of inseminated cows. Data about IFN-τ in Bos taurus indicus are still scarce. Objective of this study was to quantitatively evaluate the presence of IFN-τ during the critical period for maternal recognition of pregnancy in uterine flushings obtained in vivo by Foley catheter (Days 14, 16 and 18 post estrus) or post-mortem (Day 18 post estrus). Multiparous, cyclic or pregnant zebu cows (Bos taurus indicus) on days 14, 16 and 18 post estrus were used for in vivo or post mortem uterine flushing collection. In both cases, a Ringer solution was used to wash the uterus of cows. Uterine flushings were concentrated by ultrafiltration and lyophilized. Proteins were separated by one-dimensional electrophoresis (SDS-PAGE) in a 15% polyacrilamide gel. Interferontau quantification in uterine flushings was performed by western blotting and densitometry. Non-specific protein bands were observed in both in vivo and post mortem uterine flushings. Interferon-tau was detected only in uterine flushings obtained from pregnant cows post-mortem (P<0.05). Optical density of protein bands was not affected by the day of the critical period, state (cyclic or pregnant) or interaction day x state. There was no effect of the conceptus weight or progesterone concentration on the day of uterine flushing collection in the optical density of the IFN-τ protein band. It was concluded that the detection and quantification of IFN-τ in the uterine environment of zebu cows, in these experimental?conditions, is only possible in uterine flushings obtained post-mortem. |
id |
UNSP_e9cd7973dde0588267430fe6ab829f47 |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/70819 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Quantificação do interferon-tau durante o reconhecimento materno da gestação em fêmeas bovinas Bos taurus indicusQuantification of interferon-tau during the maternal recognition of pregnancy in Bos taurus indicus cowsBovineIFN-τPregnancyUterine environmentWestern-blottingBosBos indicusBovinaeDuring the critical period of the maternal recognition, which occurs between days 15 and 19 of pregnancy, the conceptus must competently synthesize molecules capable of blocking the synthesis of prostaglandin F2α (PGF2α) and luteolysis. In cattle, the major macromolecule involved in suck blockage is the protein interferontau (IFN-τ). During the critical period, failures in the recognition of pregnancy determine embryonic mortality on up to 40% of inseminated cows. Data about IFN-τ in Bos taurus indicus are still scarce. Objective of this study was to quantitatively evaluate the presence of IFN-τ during the critical period for maternal recognition of pregnancy in uterine flushings obtained in vivo by Foley catheter (Days 14, 16 and 18 post estrus) or post-mortem (Day 18 post estrus). Multiparous, cyclic or pregnant zebu cows (Bos taurus indicus) on days 14, 16 and 18 post estrus were used for in vivo or post mortem uterine flushing collection. In both cases, a Ringer solution was used to wash the uterus of cows. Uterine flushings were concentrated by ultrafiltration and lyophilized. Proteins were separated by one-dimensional electrophoresis (SDS-PAGE) in a 15% polyacrilamide gel. Interferontau quantification in uterine flushings was performed by western blotting and densitometry. Non-specific protein bands were observed in both in vivo and post mortem uterine flushings. Interferon-tau was detected only in uterine flushings obtained from pregnant cows post-mortem (P<0.05). Optical density of protein bands was not affected by the day of the critical period, state (cyclic or pregnant) or interaction day x state. There was no effect of the conceptus weight or progesterone concentration on the day of uterine flushing collection in the optical density of the IFN-τ protein band. It was concluded that the detection and quantification of IFN-τ in the uterine environment of zebu cows, in these experimental?conditions, is only possible in uterine flushings obtained post-mortem.Laboratório de Fisiologia e Endocrinologia Molecular Departamento de Reprodução Animal Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo-SPDepartamento de Cirurgia Faculdade de Medicina Veterinária e Zootecnia Universidade de São Paulo, São Paulo-SPLaboratório de Morfofisiologia da Placenta e do Embrião Faculdade de Zootecnia Universidade Estadual Paulista, Dracena-SPLaboratório de Morfofisiologia da Placenta e do Embrião Faculdade de Zootecnia Universidade Estadual Paulista, Dracena-SPUniversidade de São Paulo (USP)Universidade Estadual Paulista (Unesp)Giassetti, Mariana IanelloPontes, Eduardo OliveiraNiemeyer, ClaudiaSiqueira, Adriano Felipe PerezFedozzi, FilipeLima, Marcelo CardosoMarques, Vanessa BelentaniBertan, Claudia Maria [UNESP]Miglino, Maria AngélicaArruda, Rubens PaesPapa, Paula CarvalhoBinelli, Mario2014-05-27T11:23:48Z2014-05-27T11:23:48Z2008-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article111-120application/pdfhttp://www.revistas.usp.br/bjvras/article/view/26738Brazilian Journal of Veterinary Research and Animal Science, v. 45, n. SUPPL., p. 111-120, 2008.1413-95961678-4456http://hdl.handle.net/11449/708192-s2.0-781493983522-s2.0-78149398352.pdf57201624933978510000-0003-4211-0649Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPporBrazilian Journal of Veterinary Research and Animal Science0,2250,225info:eu-repo/semantics/openAccess2024-05-07T13:48:06Zoai:repositorio.unesp.br:11449/70819Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-06T00:00:02.318024Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Quantificação do interferon-tau durante o reconhecimento materno da gestação em fêmeas bovinas Bos taurus indicus Quantification of interferon-tau during the maternal recognition of pregnancy in Bos taurus indicus cows |
title |
Quantificação do interferon-tau durante o reconhecimento materno da gestação em fêmeas bovinas Bos taurus indicus |
spellingShingle |
Quantificação do interferon-tau durante o reconhecimento materno da gestação em fêmeas bovinas Bos taurus indicus Giassetti, Mariana Ianello Bovine IFN-τ Pregnancy Uterine environment Western-blotting Bos Bos indicus Bovinae |
title_short |
Quantificação do interferon-tau durante o reconhecimento materno da gestação em fêmeas bovinas Bos taurus indicus |
title_full |
Quantificação do interferon-tau durante o reconhecimento materno da gestação em fêmeas bovinas Bos taurus indicus |
title_fullStr |
Quantificação do interferon-tau durante o reconhecimento materno da gestação em fêmeas bovinas Bos taurus indicus |
title_full_unstemmed |
Quantificação do interferon-tau durante o reconhecimento materno da gestação em fêmeas bovinas Bos taurus indicus |
title_sort |
Quantificação do interferon-tau durante o reconhecimento materno da gestação em fêmeas bovinas Bos taurus indicus |
author |
Giassetti, Mariana Ianello |
author_facet |
Giassetti, Mariana Ianello Pontes, Eduardo Oliveira Niemeyer, Claudia Siqueira, Adriano Felipe Perez Fedozzi, Filipe Lima, Marcelo Cardoso Marques, Vanessa Belentani Bertan, Claudia Maria [UNESP] Miglino, Maria Angélica Arruda, Rubens Paes Papa, Paula Carvalho Binelli, Mario |
author_role |
author |
author2 |
Pontes, Eduardo Oliveira Niemeyer, Claudia Siqueira, Adriano Felipe Perez Fedozzi, Filipe Lima, Marcelo Cardoso Marques, Vanessa Belentani Bertan, Claudia Maria [UNESP] Miglino, Maria Angélica Arruda, Rubens Paes Papa, Paula Carvalho Binelli, Mario |
author2_role |
author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Giassetti, Mariana Ianello Pontes, Eduardo Oliveira Niemeyer, Claudia Siqueira, Adriano Felipe Perez Fedozzi, Filipe Lima, Marcelo Cardoso Marques, Vanessa Belentani Bertan, Claudia Maria [UNESP] Miglino, Maria Angélica Arruda, Rubens Paes Papa, Paula Carvalho Binelli, Mario |
dc.subject.por.fl_str_mv |
Bovine IFN-τ Pregnancy Uterine environment Western-blotting Bos Bos indicus Bovinae |
topic |
Bovine IFN-τ Pregnancy Uterine environment Western-blotting Bos Bos indicus Bovinae |
description |
During the critical period of the maternal recognition, which occurs between days 15 and 19 of pregnancy, the conceptus must competently synthesize molecules capable of blocking the synthesis of prostaglandin F2α (PGF2α) and luteolysis. In cattle, the major macromolecule involved in suck blockage is the protein interferontau (IFN-τ). During the critical period, failures in the recognition of pregnancy determine embryonic mortality on up to 40% of inseminated cows. Data about IFN-τ in Bos taurus indicus are still scarce. Objective of this study was to quantitatively evaluate the presence of IFN-τ during the critical period for maternal recognition of pregnancy in uterine flushings obtained in vivo by Foley catheter (Days 14, 16 and 18 post estrus) or post-mortem (Day 18 post estrus). Multiparous, cyclic or pregnant zebu cows (Bos taurus indicus) on days 14, 16 and 18 post estrus were used for in vivo or post mortem uterine flushing collection. In both cases, a Ringer solution was used to wash the uterus of cows. Uterine flushings were concentrated by ultrafiltration and lyophilized. Proteins were separated by one-dimensional electrophoresis (SDS-PAGE) in a 15% polyacrilamide gel. Interferontau quantification in uterine flushings was performed by western blotting and densitometry. Non-specific protein bands were observed in both in vivo and post mortem uterine flushings. Interferon-tau was detected only in uterine flushings obtained from pregnant cows post-mortem (P<0.05). Optical density of protein bands was not affected by the day of the critical period, state (cyclic or pregnant) or interaction day x state. There was no effect of the conceptus weight or progesterone concentration on the day of uterine flushing collection in the optical density of the IFN-τ protein band. It was concluded that the detection and quantification of IFN-τ in the uterine environment of zebu cows, in these experimental?conditions, is only possible in uterine flushings obtained post-mortem. |
publishDate |
2008 |
dc.date.none.fl_str_mv |
2008-12-01 2014-05-27T11:23:48Z 2014-05-27T11:23:48Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.revistas.usp.br/bjvras/article/view/26738 Brazilian Journal of Veterinary Research and Animal Science, v. 45, n. SUPPL., p. 111-120, 2008. 1413-9596 1678-4456 http://hdl.handle.net/11449/70819 2-s2.0-78149398352 2-s2.0-78149398352.pdf 5720162493397851 0000-0003-4211-0649 |
url |
http://www.revistas.usp.br/bjvras/article/view/26738 http://hdl.handle.net/11449/70819 |
identifier_str_mv |
Brazilian Journal of Veterinary Research and Animal Science, v. 45, n. SUPPL., p. 111-120, 2008. 1413-9596 1678-4456 2-s2.0-78149398352 2-s2.0-78149398352.pdf 5720162493397851 0000-0003-4211-0649 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
Brazilian Journal of Veterinary Research and Animal Science 0,225 0,225 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
111-120 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129570912600064 |