Ocorrência de Porphyromonas gingivalis na microbiota bucal de pacientes submetidos à radioterapia para tratamento de lesões malignas de cabeça e pescoço

• Autor(a) principal: Geraldes, Aline Martucci [UNESP]
• Data de Publicação: 2010
• Tipo de documento: Trabalho de conclusão de curso
• Idioma: por
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://hdl.handle.net/11449/149293; http://www.athena.biblioteca.unesp.br/exlibris/bd/capelo/2017-01-25/000847973.pdf
• topic: Radioterapia; Porphyromonas gingivalis; Reação em cadeia da polimerase; Radiotherapy

Radiotherapy is employed for the treatment of head and neck cancer with favorable results. However, the side effects of radiation on salivary glands and other oral tissues may create favorable conditions for the establishment and proliferation of oral microorganisms. This investigation evaluated the occurrence of Porphyromonas gingivalis in the oral microbiota of patients undergoing radiotherapy for treatment of head and neck cancer. After clinical examinations, samples of saliva, dental biofilm and surfaces of oral mucosa were collected, and this procedure was performed at different moments during treatment, as well as 6 months after the conclusion of radiotherapy. The presence of these anaerobes was carried out through culture using agar supplemented with hemin, menadione and defibrillated horse blood. It was verified and identification of isolates was performed by commercial kits. Next, the antimicrobial susceptibility and typing of the fimA gene were determined. Patients irradiated after the start of radiotherapy developed severe mucositis, dermatitis, dysgeusia, xerostomia and to a lesser extent, oral candidiasis. A high occurrence of anaerobic samples from patients with periodontitis and to a lesser extent, gingivitis was observed. The prevalence and populations of P. gingivalis increased significantly after the start of radiotherapy and remained so, more or less stable throughout the study period, and the distribution and populations of anaerobic showed positive correlation with xerostomia. The isolates werw susceptible or moderately susceptible to all antimicrobials and radiotherapy did not alter the proportions between the different genotypes of P. gingivalis in accordance with the fimA gene