Nucleotide and phylogenetic analysis of human papillomavirus types 6 and 11 isolated from recurrent respiratory papillomatosis in Brazil

• Autor(a) principal: de Matos, Renata Prandini Adum [UNESP]
• Data de Publicação: 2013
• Outros Autores: Sichero, Laura, Mansur, Isabela Mazuco [UNESP], do Bonfim, Caroline Measso [UNESP], Bittar, Cíntia [UNESP], Nogueira, Rodrigo Lacerda, Küpper, Daniel Salgado, Valera, Fabiana Cardoso Pereira, Nogueira, Maurício Lacerda, Villa, Luisa Lina, Calmon, Marilia Freitas [UNESP], Rahal, Paula [UNESP]
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://dx.doi.org/10.1016/j.meegid.2012.12.033; http://hdl.handle.net/11449/75522
• topic: HPV variants; HPV-11; HPV-6; Phylogenetic analysis; Recurrent respiratory papillomatosis; protein E6; adolescent; adult; amino acid substitution; Brazil; child; clinical article; cohort analysis; condyloma acuminatum; controlled study; disease severity; E6 early gene; female; genetic variability; human; Human papillomavirus type 11; Human papillomavirus type 6; larynx papillomatosis; lung tumor; male; molecular cloning; nonhuman; nucleic acid analysis; nucleotide sequence; papillomatosis; phylogenetic tree; phylogeny; point mutation; polymerase chain reaction; preschool child; priority journal; school child; sequence analysis; tumor recurrence; uterine cervix cancer; virus isolation

There are few studies about the distribution of natural molecular variants of low-risk HPVs. Our aim was to evaluate the E6 early gene variability among HPV-6 and HPV-11 isolates detected in recurrent respiratory papillomatosis (RRP) samples obtained in a cohort of Brazilian patients. We also performed a phylogenetic analysis in order to compare nucleotide sequences identified in our study with previously reported isolates from different anatomic sites (laryngeal papillomas, genital warts, cervical cancer and anal swabs) obtained from other parts of the world to determine the phylogenetic relationships of variants detected in Brazil. The complete coding region of the E6 gene of 25 samples was cloned and sequenced: 18 isolates of HPV-6 (72%) and 7 isolates of HPV-11 (28%). A total of four different HPV-6 genomic variants and two HPV-11 genomic variants was identified. It was not possible to correlate specific variants with disease severity. Phylogenetic trees for both HPV types were constructed enclosing both E6 sequences detected in our study and formerly published sequences. In both phylogenetic trees, the sequences from Brazil did not group together. We could not establish a geographical association between HPV-6 or HPV-11 variants, unlike HPV-16 and HPV-18. © 2013 Elsevier B.V.