In vitro dentin permeability and tubule occlusion of experimental in-office desensitizing materials

Detalhes bibliográficos
Autor(a) principal: de Castro Oliveira, Laryssa [UNESP]
Data de Publicação: 2023
Outros Autores: Marchetti, Vitória Marega [UNESP], de Souza e Silva Ramos, Fernanda [UNESP], Delbem, Alberto Carlos Botazzo [UNESP], Souza, Marina Trevelin, Ganss, Bernhard, Theodoro, Leticia Helena [UNESP], Fagundes, Ticiane Cestari [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1007/s00784-022-04760-y
http://hdl.handle.net/11449/249319
Resumo: Objectives: This study investigates the dentin permeability (by hydraulic conductance) and tubule occlusion (by confocal and scanning electron microscopies) of in-office desensitizing materials. Materials and methods: Bovine dentin blocks were immersed in EDTA to open dentinal tubules. Placebo varnish (PLA), fluoride varnish (FLU), NaF 5% + 5% nanoparticulate sodium trimetaphosphate varnish (TMP), universal adhesive system (SBU), S-PRG filler varnish (SPRG), Biosilicate (BIOS), and amelotin (AMTN) solution were the materials tested. After application, the specimens underwent an erosive-abrasive challenge. Dentin permeability was evaluated at T0 (initial), T1 (after treatment), and T2 (after challenge). Confocal and scanning electron microscopy (SEM) were used to evaluate, respectively, length and number of dentinal tubule occlusions and opened dentinal tubules, after challenge. Permeability and SEM data were analyzed by two-way repeated measures ANOVA and Tukey’s tests. Confocal data were analyzed by one-way ANOVA, Tukey’s test, and Kruskal–Wallis and Dunn’s tests. Spearman and Pearson’s correlation tests were also used. Significance level was set at 5%. Results: At T1, the AMTN group showed the lowest permeability value, following the increasing order at T2: AMTN = SBU < BIOS = SPRG < TMP < FLU < PLA. The SBU group had the highest value of occluded dentinal tubule length. The AMTN group presented more occluded dentinal tubules compared to PLA and FLU. AMTN and SBU had the lowest values of opened dentin tubules. Results showed a negative correlation between the analyses. Conclusion: The SBU and AMTN solution were more effective in reducing dentin permeability by occluding dentin tubules. Clinical relevance: All materials reduced permeability after challenge, except fluoride varnish.
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spelling In vitro dentin permeability and tubule occlusion of experimental in-office desensitizing materialsDentin desensitizerDentin hypersensitivityDentin permeabilityDentinal tubule occlusionObjectives: This study investigates the dentin permeability (by hydraulic conductance) and tubule occlusion (by confocal and scanning electron microscopies) of in-office desensitizing materials. Materials and methods: Bovine dentin blocks were immersed in EDTA to open dentinal tubules. Placebo varnish (PLA), fluoride varnish (FLU), NaF 5% + 5% nanoparticulate sodium trimetaphosphate varnish (TMP), universal adhesive system (SBU), S-PRG filler varnish (SPRG), Biosilicate (BIOS), and amelotin (AMTN) solution were the materials tested. After application, the specimens underwent an erosive-abrasive challenge. Dentin permeability was evaluated at T0 (initial), T1 (after treatment), and T2 (after challenge). Confocal and scanning electron microscopy (SEM) were used to evaluate, respectively, length and number of dentinal tubule occlusions and opened dentinal tubules, after challenge. Permeability and SEM data were analyzed by two-way repeated measures ANOVA and Tukey’s tests. Confocal data were analyzed by one-way ANOVA, Tukey’s test, and Kruskal–Wallis and Dunn’s tests. Spearman and Pearson’s correlation tests were also used. Significance level was set at 5%. Results: At T1, the AMTN group showed the lowest permeability value, following the increasing order at T2: AMTN = SBU < BIOS = SPRG < TMP < FLU < PLA. The SBU group had the highest value of occluded dentinal tubule length. The AMTN group presented more occluded dentinal tubules compared to PLA and FLU. AMTN and SBU had the lowest values of opened dentin tubules. Results showed a negative correlation between the analyses. Conclusion: The SBU and AMTN solution were more effective in reducing dentin permeability by occluding dentin tubules. Clinical relevance: All materials reduced permeability after challenge, except fluoride varnish.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Department of Preventive and Restorative Dentistry School of Dentistry São Paulo State University (UNESP), CEPVitreous Materials Laboratory Department of Materials Engineering Federal University of São CarlosFaculty of Dentistry and Institute of Biomedical Engineering University of TorontoDepartment of Diagnosis and Surgery Division of Periodontics School of Dentistry São Paulo State University (UNESP)Department of Preventive and Restorative Dentistry School of Dentistry São Paulo State University (UNESP), CEPDepartment of Diagnosis and Surgery Division of Periodontics School of Dentistry São Paulo State University (UNESP)FAPESP: 2020/07625-0CAPES: 88887.310463/2018-00Universidade Estadual Paulista (UNESP)Universidade Federal de São Carlos (UFSCar)University of Torontode Castro Oliveira, Laryssa [UNESP]Marchetti, Vitória Marega [UNESP]de Souza e Silva Ramos, Fernanda [UNESP]Delbem, Alberto Carlos Botazzo [UNESP]Souza, Marina TrevelinGanss, BernhardTheodoro, Leticia Helena [UNESP]Fagundes, Ticiane Cestari [UNESP]2023-07-29T15:12:53Z2023-07-29T15:12:53Z2023-03-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1265-1276http://dx.doi.org/10.1007/s00784-022-04760-yClinical Oral Investigations, v. 27, n. 3, p. 1265-1276, 2023.1436-37711432-6981http://hdl.handle.net/11449/24931910.1007/s00784-022-04760-y2-s2.0-85140930694Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengClinical Oral Investigationsinfo:eu-repo/semantics/openAccess2024-09-19T17:56:24Zoai:repositorio.unesp.br:11449/249319Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-19T17:56:24Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv In vitro dentin permeability and tubule occlusion of experimental in-office desensitizing materials
title In vitro dentin permeability and tubule occlusion of experimental in-office desensitizing materials
spellingShingle In vitro dentin permeability and tubule occlusion of experimental in-office desensitizing materials
de Castro Oliveira, Laryssa [UNESP]
Dentin desensitizer
Dentin hypersensitivity
Dentin permeability
Dentinal tubule occlusion
title_short In vitro dentin permeability and tubule occlusion of experimental in-office desensitizing materials
title_full In vitro dentin permeability and tubule occlusion of experimental in-office desensitizing materials
title_fullStr In vitro dentin permeability and tubule occlusion of experimental in-office desensitizing materials
title_full_unstemmed In vitro dentin permeability and tubule occlusion of experimental in-office desensitizing materials
title_sort In vitro dentin permeability and tubule occlusion of experimental in-office desensitizing materials
author de Castro Oliveira, Laryssa [UNESP]
author_facet de Castro Oliveira, Laryssa [UNESP]
Marchetti, Vitória Marega [UNESP]
de Souza e Silva Ramos, Fernanda [UNESP]
Delbem, Alberto Carlos Botazzo [UNESP]
Souza, Marina Trevelin
Ganss, Bernhard
Theodoro, Leticia Helena [UNESP]
Fagundes, Ticiane Cestari [UNESP]
author_role author
author2 Marchetti, Vitória Marega [UNESP]
de Souza e Silva Ramos, Fernanda [UNESP]
Delbem, Alberto Carlos Botazzo [UNESP]
Souza, Marina Trevelin
Ganss, Bernhard
Theodoro, Leticia Helena [UNESP]
Fagundes, Ticiane Cestari [UNESP]
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (UNESP)
Universidade Federal de São Carlos (UFSCar)
University of Toronto
dc.contributor.author.fl_str_mv de Castro Oliveira, Laryssa [UNESP]
Marchetti, Vitória Marega [UNESP]
de Souza e Silva Ramos, Fernanda [UNESP]
Delbem, Alberto Carlos Botazzo [UNESP]
Souza, Marina Trevelin
Ganss, Bernhard
Theodoro, Leticia Helena [UNESP]
Fagundes, Ticiane Cestari [UNESP]
dc.subject.por.fl_str_mv Dentin desensitizer
Dentin hypersensitivity
Dentin permeability
Dentinal tubule occlusion
topic Dentin desensitizer
Dentin hypersensitivity
Dentin permeability
Dentinal tubule occlusion
description Objectives: This study investigates the dentin permeability (by hydraulic conductance) and tubule occlusion (by confocal and scanning electron microscopies) of in-office desensitizing materials. Materials and methods: Bovine dentin blocks were immersed in EDTA to open dentinal tubules. Placebo varnish (PLA), fluoride varnish (FLU), NaF 5% + 5% nanoparticulate sodium trimetaphosphate varnish (TMP), universal adhesive system (SBU), S-PRG filler varnish (SPRG), Biosilicate (BIOS), and amelotin (AMTN) solution were the materials tested. After application, the specimens underwent an erosive-abrasive challenge. Dentin permeability was evaluated at T0 (initial), T1 (after treatment), and T2 (after challenge). Confocal and scanning electron microscopy (SEM) were used to evaluate, respectively, length and number of dentinal tubule occlusions and opened dentinal tubules, after challenge. Permeability and SEM data were analyzed by two-way repeated measures ANOVA and Tukey’s tests. Confocal data were analyzed by one-way ANOVA, Tukey’s test, and Kruskal–Wallis and Dunn’s tests. Spearman and Pearson’s correlation tests were also used. Significance level was set at 5%. Results: At T1, the AMTN group showed the lowest permeability value, following the increasing order at T2: AMTN = SBU < BIOS = SPRG < TMP < FLU < PLA. The SBU group had the highest value of occluded dentinal tubule length. The AMTN group presented more occluded dentinal tubules compared to PLA and FLU. AMTN and SBU had the lowest values of opened dentin tubules. Results showed a negative correlation between the analyses. Conclusion: The SBU and AMTN solution were more effective in reducing dentin permeability by occluding dentin tubules. Clinical relevance: All materials reduced permeability after challenge, except fluoride varnish.
publishDate 2023
dc.date.none.fl_str_mv 2023-07-29T15:12:53Z
2023-07-29T15:12:53Z
2023-03-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1007/s00784-022-04760-y
Clinical Oral Investigations, v. 27, n. 3, p. 1265-1276, 2023.
1436-3771
1432-6981
http://hdl.handle.net/11449/249319
10.1007/s00784-022-04760-y
2-s2.0-85140930694
url http://dx.doi.org/10.1007/s00784-022-04760-y
http://hdl.handle.net/11449/249319
identifier_str_mv Clinical Oral Investigations, v. 27, n. 3, p. 1265-1276, 2023.
1436-3771
1432-6981
10.1007/s00784-022-04760-y
2-s2.0-85140930694
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Clinical Oral Investigations
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 1265-1276
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv repositoriounesp@unesp.br
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