In vitro dentin permeability and tubule occlusion of experimental in-office desensitizing materials
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s00784-022-04760-y http://hdl.handle.net/11449/249319 |
Resumo: | Objectives: This study investigates the dentin permeability (by hydraulic conductance) and tubule occlusion (by confocal and scanning electron microscopies) of in-office desensitizing materials. Materials and methods: Bovine dentin blocks were immersed in EDTA to open dentinal tubules. Placebo varnish (PLA), fluoride varnish (FLU), NaF 5% + 5% nanoparticulate sodium trimetaphosphate varnish (TMP), universal adhesive system (SBU), S-PRG filler varnish (SPRG), Biosilicate (BIOS), and amelotin (AMTN) solution were the materials tested. After application, the specimens underwent an erosive-abrasive challenge. Dentin permeability was evaluated at T0 (initial), T1 (after treatment), and T2 (after challenge). Confocal and scanning electron microscopy (SEM) were used to evaluate, respectively, length and number of dentinal tubule occlusions and opened dentinal tubules, after challenge. Permeability and SEM data were analyzed by two-way repeated measures ANOVA and Tukey’s tests. Confocal data were analyzed by one-way ANOVA, Tukey’s test, and Kruskal–Wallis and Dunn’s tests. Spearman and Pearson’s correlation tests were also used. Significance level was set at 5%. Results: At T1, the AMTN group showed the lowest permeability value, following the increasing order at T2: AMTN = SBU < BIOS = SPRG < TMP < FLU < PLA. The SBU group had the highest value of occluded dentinal tubule length. The AMTN group presented more occluded dentinal tubules compared to PLA and FLU. AMTN and SBU had the lowest values of opened dentin tubules. Results showed a negative correlation between the analyses. Conclusion: The SBU and AMTN solution were more effective in reducing dentin permeability by occluding dentin tubules. Clinical relevance: All materials reduced permeability after challenge, except fluoride varnish. |
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In vitro dentin permeability and tubule occlusion of experimental in-office desensitizing materialsDentin desensitizerDentin hypersensitivityDentin permeabilityDentinal tubule occlusionObjectives: This study investigates the dentin permeability (by hydraulic conductance) and tubule occlusion (by confocal and scanning electron microscopies) of in-office desensitizing materials. Materials and methods: Bovine dentin blocks were immersed in EDTA to open dentinal tubules. Placebo varnish (PLA), fluoride varnish (FLU), NaF 5% + 5% nanoparticulate sodium trimetaphosphate varnish (TMP), universal adhesive system (SBU), S-PRG filler varnish (SPRG), Biosilicate (BIOS), and amelotin (AMTN) solution were the materials tested. After application, the specimens underwent an erosive-abrasive challenge. Dentin permeability was evaluated at T0 (initial), T1 (after treatment), and T2 (after challenge). Confocal and scanning electron microscopy (SEM) were used to evaluate, respectively, length and number of dentinal tubule occlusions and opened dentinal tubules, after challenge. Permeability and SEM data were analyzed by two-way repeated measures ANOVA and Tukey’s tests. Confocal data were analyzed by one-way ANOVA, Tukey’s test, and Kruskal–Wallis and Dunn’s tests. Spearman and Pearson’s correlation tests were also used. Significance level was set at 5%. Results: At T1, the AMTN group showed the lowest permeability value, following the increasing order at T2: AMTN = SBU < BIOS = SPRG < TMP < FLU < PLA. The SBU group had the highest value of occluded dentinal tubule length. The AMTN group presented more occluded dentinal tubules compared to PLA and FLU. AMTN and SBU had the lowest values of opened dentin tubules. Results showed a negative correlation between the analyses. Conclusion: The SBU and AMTN solution were more effective in reducing dentin permeability by occluding dentin tubules. Clinical relevance: All materials reduced permeability after challenge, except fluoride varnish.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Department of Preventive and Restorative Dentistry School of Dentistry São Paulo State University (UNESP), CEPVitreous Materials Laboratory Department of Materials Engineering Federal University of São CarlosFaculty of Dentistry and Institute of Biomedical Engineering University of TorontoDepartment of Diagnosis and Surgery Division of Periodontics School of Dentistry São Paulo State University (UNESP)Department of Preventive and Restorative Dentistry School of Dentistry São Paulo State University (UNESP), CEPDepartment of Diagnosis and Surgery Division of Periodontics School of Dentistry São Paulo State University (UNESP)FAPESP: 2020/07625-0CAPES: 88887.310463/2018-00Universidade Estadual Paulista (UNESP)Universidade Federal de São Carlos (UFSCar)University of Torontode Castro Oliveira, Laryssa [UNESP]Marchetti, Vitória Marega [UNESP]de Souza e Silva Ramos, Fernanda [UNESP]Delbem, Alberto Carlos Botazzo [UNESP]Souza, Marina TrevelinGanss, BernhardTheodoro, Leticia Helena [UNESP]Fagundes, Ticiane Cestari [UNESP]2023-07-29T15:12:53Z2023-07-29T15:12:53Z2023-03-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1265-1276http://dx.doi.org/10.1007/s00784-022-04760-yClinical Oral Investigations, v. 27, n. 3, p. 1265-1276, 2023.1436-37711432-6981http://hdl.handle.net/11449/24931910.1007/s00784-022-04760-y2-s2.0-85140930694Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengClinical Oral Investigationsinfo:eu-repo/semantics/openAccess2024-09-19T17:56:24Zoai:repositorio.unesp.br:11449/249319Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-19T17:56:24Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
In vitro dentin permeability and tubule occlusion of experimental in-office desensitizing materials |
title |
In vitro dentin permeability and tubule occlusion of experimental in-office desensitizing materials |
spellingShingle |
In vitro dentin permeability and tubule occlusion of experimental in-office desensitizing materials de Castro Oliveira, Laryssa [UNESP] Dentin desensitizer Dentin hypersensitivity Dentin permeability Dentinal tubule occlusion |
title_short |
In vitro dentin permeability and tubule occlusion of experimental in-office desensitizing materials |
title_full |
In vitro dentin permeability and tubule occlusion of experimental in-office desensitizing materials |
title_fullStr |
In vitro dentin permeability and tubule occlusion of experimental in-office desensitizing materials |
title_full_unstemmed |
In vitro dentin permeability and tubule occlusion of experimental in-office desensitizing materials |
title_sort |
In vitro dentin permeability and tubule occlusion of experimental in-office desensitizing materials |
author |
de Castro Oliveira, Laryssa [UNESP] |
author_facet |
de Castro Oliveira, Laryssa [UNESP] Marchetti, Vitória Marega [UNESP] de Souza e Silva Ramos, Fernanda [UNESP] Delbem, Alberto Carlos Botazzo [UNESP] Souza, Marina Trevelin Ganss, Bernhard Theodoro, Leticia Helena [UNESP] Fagundes, Ticiane Cestari [UNESP] |
author_role |
author |
author2 |
Marchetti, Vitória Marega [UNESP] de Souza e Silva Ramos, Fernanda [UNESP] Delbem, Alberto Carlos Botazzo [UNESP] Souza, Marina Trevelin Ganss, Bernhard Theodoro, Leticia Helena [UNESP] Fagundes, Ticiane Cestari [UNESP] |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) Universidade Federal de São Carlos (UFSCar) University of Toronto |
dc.contributor.author.fl_str_mv |
de Castro Oliveira, Laryssa [UNESP] Marchetti, Vitória Marega [UNESP] de Souza e Silva Ramos, Fernanda [UNESP] Delbem, Alberto Carlos Botazzo [UNESP] Souza, Marina Trevelin Ganss, Bernhard Theodoro, Leticia Helena [UNESP] Fagundes, Ticiane Cestari [UNESP] |
dc.subject.por.fl_str_mv |
Dentin desensitizer Dentin hypersensitivity Dentin permeability Dentinal tubule occlusion |
topic |
Dentin desensitizer Dentin hypersensitivity Dentin permeability Dentinal tubule occlusion |
description |
Objectives: This study investigates the dentin permeability (by hydraulic conductance) and tubule occlusion (by confocal and scanning electron microscopies) of in-office desensitizing materials. Materials and methods: Bovine dentin blocks were immersed in EDTA to open dentinal tubules. Placebo varnish (PLA), fluoride varnish (FLU), NaF 5% + 5% nanoparticulate sodium trimetaphosphate varnish (TMP), universal adhesive system (SBU), S-PRG filler varnish (SPRG), Biosilicate (BIOS), and amelotin (AMTN) solution were the materials tested. After application, the specimens underwent an erosive-abrasive challenge. Dentin permeability was evaluated at T0 (initial), T1 (after treatment), and T2 (after challenge). Confocal and scanning electron microscopy (SEM) were used to evaluate, respectively, length and number of dentinal tubule occlusions and opened dentinal tubules, after challenge. Permeability and SEM data were analyzed by two-way repeated measures ANOVA and Tukey’s tests. Confocal data were analyzed by one-way ANOVA, Tukey’s test, and Kruskal–Wallis and Dunn’s tests. Spearman and Pearson’s correlation tests were also used. Significance level was set at 5%. Results: At T1, the AMTN group showed the lowest permeability value, following the increasing order at T2: AMTN = SBU < BIOS = SPRG < TMP < FLU < PLA. The SBU group had the highest value of occluded dentinal tubule length. The AMTN group presented more occluded dentinal tubules compared to PLA and FLU. AMTN and SBU had the lowest values of opened dentin tubules. Results showed a negative correlation between the analyses. Conclusion: The SBU and AMTN solution were more effective in reducing dentin permeability by occluding dentin tubules. Clinical relevance: All materials reduced permeability after challenge, except fluoride varnish. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-07-29T15:12:53Z 2023-07-29T15:12:53Z 2023-03-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s00784-022-04760-y Clinical Oral Investigations, v. 27, n. 3, p. 1265-1276, 2023. 1436-3771 1432-6981 http://hdl.handle.net/11449/249319 10.1007/s00784-022-04760-y 2-s2.0-85140930694 |
url |
http://dx.doi.org/10.1007/s00784-022-04760-y http://hdl.handle.net/11449/249319 |
identifier_str_mv |
Clinical Oral Investigations, v. 27, n. 3, p. 1265-1276, 2023. 1436-3771 1432-6981 10.1007/s00784-022-04760-y 2-s2.0-85140930694 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Clinical Oral Investigations |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1265-1276 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
repositoriounesp@unesp.br |
_version_ |
1813546424003985408 |