Diversity of Anaplasma species in cattle in Mozambique
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.ttbdis.2019.02.012 http://hdl.handle.net/11449/190148 |
Resumo: | Although species of Anaplasma are highly prevalent Rickettsiales agents in domestic and wild ruminants with a wide distribution worldwide, few studies have been conducted so far to detect and/or investigate the diversity of these agentsin cattle in Mozambique. In the present study, serological and molecular assays were used to investigate the occurrence of Anaplasma spp. in 219 bovines sampled in the districts of Boane, Magude, Matutuíne, Moamba and Namaacha in Maputo, Mozambique. In the iELISA test for detection ofIgG antibodies to A. marginale, 86.3% (189/219) of the samples were positive. In qPCR assays for the gene msp1β for A. marginale and msp2 for A. phagocytophilum, 97.3% (213/219) and 2.7% (6/219) of the animals were positive, respectively. Two different cPCR protocols based on the 16S rRNA gene showed that 100% of the samples were positive for Anaplasma spp. The DNA sequences obtained were phylogenetically related to A. platys, A. phagocytophilum, Candidatus Anaplasma boleense, A. centrale, A. marginale and A. ovis. Phylogenetic inference based on the msp4 and msp5 genes positioned the obtained sequences in the clade of A. marginale, with evidence of occurrence of 8 and 5 different haplotypes for each gene, respectively. Anaplasma sp. phylogenetically associated with A. platys was evidenced in phylogenetic analyzes based on 16S rRNA and groEL genes. It is concluded that a high diversity of species of Anaplasma spp. occurs in cattle in Mozambique. |
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Diversity of Anaplasma species in cattle in MozambiqueAnaplasma centraleAnaplasma marginaleAnaplasma ovisAnaplasma phagocytophilumAnaplasma platys‘Candidatus Anaplasma boleense’Although species of Anaplasma are highly prevalent Rickettsiales agents in domestic and wild ruminants with a wide distribution worldwide, few studies have been conducted so far to detect and/or investigate the diversity of these agentsin cattle in Mozambique. In the present study, serological and molecular assays were used to investigate the occurrence of Anaplasma spp. in 219 bovines sampled in the districts of Boane, Magude, Matutuíne, Moamba and Namaacha in Maputo, Mozambique. In the iELISA test for detection ofIgG antibodies to A. marginale, 86.3% (189/219) of the samples were positive. In qPCR assays for the gene msp1β for A. marginale and msp2 for A. phagocytophilum, 97.3% (213/219) and 2.7% (6/219) of the animals were positive, respectively. Two different cPCR protocols based on the 16S rRNA gene showed that 100% of the samples were positive for Anaplasma spp. The DNA sequences obtained were phylogenetically related to A. platys, A. phagocytophilum, Candidatus Anaplasma boleense, A. centrale, A. marginale and A. ovis. Phylogenetic inference based on the msp4 and msp5 genes positioned the obtained sequences in the clade of A. marginale, with evidence of occurrence of 8 and 5 different haplotypes for each gene, respectively. Anaplasma sp. phylogenetically associated with A. platys was evidenced in phylogenetic analyzes based on 16S rRNA and groEL genes. It is concluded that a high diversity of species of Anaplasma spp. occurs in cattle in Mozambique.Fundação de Amparo à Pesquisa do Estado de GoiásPaulista State University (Unesp)Universidade Estadual Paulista (Unesp) Faculty of Agrarian and Veterinary SciencesDirectorate of Animal SciencesImunodot Diagnósticos LtdaPaulista State University (Unesp)Universidade Estadual Paulista (Unesp) Faculty of Agrarian and Veterinary SciencesFundação de Amparo à Pesquisa do Estado de Goiás: 2015/14896-1Universidade Estadual Paulista (Unesp)Directorate of Animal SciencesImunodot Diagnósticos LtdaFernandes, Simone de Jesus [UNESP]Matos, Carlos António [UNESP]Freschi, Carla Robertade Souza Ramos, Inalda Angélica [UNESP]Machado, Rosangela Zacarias [UNESP]André, Marcos Rogério [UNESP]2019-10-06T17:03:52Z2019-10-06T17:03:52Z2019-04-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article651-664http://dx.doi.org/10.1016/j.ttbdis.2019.02.012Ticks and Tick-borne Diseases, v. 10, n. 3, p. 651-664, 2019.1877-96031877-959Xhttp://hdl.handle.net/11449/19014810.1016/j.ttbdis.2019.02.0122-s2.0-850622323089139899895580513Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengTicks and Tick-borne Diseasesinfo:eu-repo/semantics/openAccess2024-06-07T13:02:16Zoai:repositorio.unesp.br:11449/190148Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T18:19:22.191951Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Diversity of Anaplasma species in cattle in Mozambique |
title |
Diversity of Anaplasma species in cattle in Mozambique |
spellingShingle |
Diversity of Anaplasma species in cattle in Mozambique Fernandes, Simone de Jesus [UNESP] Anaplasma centrale Anaplasma marginale Anaplasma ovis Anaplasma phagocytophilum Anaplasma platys ‘Candidatus Anaplasma boleense’ |
title_short |
Diversity of Anaplasma species in cattle in Mozambique |
title_full |
Diversity of Anaplasma species in cattle in Mozambique |
title_fullStr |
Diversity of Anaplasma species in cattle in Mozambique |
title_full_unstemmed |
Diversity of Anaplasma species in cattle in Mozambique |
title_sort |
Diversity of Anaplasma species in cattle in Mozambique |
author |
Fernandes, Simone de Jesus [UNESP] |
author_facet |
Fernandes, Simone de Jesus [UNESP] Matos, Carlos António [UNESP] Freschi, Carla Roberta de Souza Ramos, Inalda Angélica [UNESP] Machado, Rosangela Zacarias [UNESP] André, Marcos Rogério [UNESP] |
author_role |
author |
author2 |
Matos, Carlos António [UNESP] Freschi, Carla Roberta de Souza Ramos, Inalda Angélica [UNESP] Machado, Rosangela Zacarias [UNESP] André, Marcos Rogério [UNESP] |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Directorate of Animal Sciences Imunodot Diagnósticos Ltda |
dc.contributor.author.fl_str_mv |
Fernandes, Simone de Jesus [UNESP] Matos, Carlos António [UNESP] Freschi, Carla Roberta de Souza Ramos, Inalda Angélica [UNESP] Machado, Rosangela Zacarias [UNESP] André, Marcos Rogério [UNESP] |
dc.subject.por.fl_str_mv |
Anaplasma centrale Anaplasma marginale Anaplasma ovis Anaplasma phagocytophilum Anaplasma platys ‘Candidatus Anaplasma boleense’ |
topic |
Anaplasma centrale Anaplasma marginale Anaplasma ovis Anaplasma phagocytophilum Anaplasma platys ‘Candidatus Anaplasma boleense’ |
description |
Although species of Anaplasma are highly prevalent Rickettsiales agents in domestic and wild ruminants with a wide distribution worldwide, few studies have been conducted so far to detect and/or investigate the diversity of these agentsin cattle in Mozambique. In the present study, serological and molecular assays were used to investigate the occurrence of Anaplasma spp. in 219 bovines sampled in the districts of Boane, Magude, Matutuíne, Moamba and Namaacha in Maputo, Mozambique. In the iELISA test for detection ofIgG antibodies to A. marginale, 86.3% (189/219) of the samples were positive. In qPCR assays for the gene msp1β for A. marginale and msp2 for A. phagocytophilum, 97.3% (213/219) and 2.7% (6/219) of the animals were positive, respectively. Two different cPCR protocols based on the 16S rRNA gene showed that 100% of the samples were positive for Anaplasma spp. The DNA sequences obtained were phylogenetically related to A. platys, A. phagocytophilum, Candidatus Anaplasma boleense, A. centrale, A. marginale and A. ovis. Phylogenetic inference based on the msp4 and msp5 genes positioned the obtained sequences in the clade of A. marginale, with evidence of occurrence of 8 and 5 different haplotypes for each gene, respectively. Anaplasma sp. phylogenetically associated with A. platys was evidenced in phylogenetic analyzes based on 16S rRNA and groEL genes. It is concluded that a high diversity of species of Anaplasma spp. occurs in cattle in Mozambique. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-10-06T17:03:52Z 2019-10-06T17:03:52Z 2019-04-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.ttbdis.2019.02.012 Ticks and Tick-borne Diseases, v. 10, n. 3, p. 651-664, 2019. 1877-9603 1877-959X http://hdl.handle.net/11449/190148 10.1016/j.ttbdis.2019.02.012 2-s2.0-85062232308 9139899895580513 |
url |
http://dx.doi.org/10.1016/j.ttbdis.2019.02.012 http://hdl.handle.net/11449/190148 |
identifier_str_mv |
Ticks and Tick-borne Diseases, v. 10, n. 3, p. 651-664, 2019. 1877-9603 1877-959X 10.1016/j.ttbdis.2019.02.012 2-s2.0-85062232308 9139899895580513 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Ticks and Tick-borne Diseases |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
651-664 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808128919378853888 |