Influence of sucrose on growth and sensitivity of Candida albicans alone and in combination with Enterococcus faecalis and Streptococcus mutans to photodynamic therapy

Detalhes bibliográficos
Autor(a) principal: Tomé, Fernanda Malagutti [UNESP]
Data de Publicação: 2017
Outros Autores: Paula Ramos, Lucas De [UNESP], Freire, Fernanda [UNESP], Pereira, Cristiane Aparecida [UNESP], de Oliveira, Ingrid Christine Barbosa [UNESP], Junqueira, Juliana Campos [UNESP], Jorge, Antonio Olavo Cardoso [UNESP], Oliveira, Luciane Dias de [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1007/s10103-017-2201-2
http://hdl.handle.net/11449/174436
Resumo: This study has evaluated the effects of photodynamic inactivation (PDI) using erythrosine as photosensitizer and green light-emitting diode (LED) on biofilms of Candida albicans alone and in combination with Enterococcus faecalis and Streptococcus mutans. We have also evaluated the effect of sucrose on biofilm formation and bacterial growth and sensitivity to PDI. Biofilms were formed in suspension of 106 cells/ml on plates before being grown in broth culture with and without sucrose and incubated for 48 h. Next, the treatment was applied using erythrosine at a concentration of 400 μM for 5 min and green LED (532 ± 10 nm) for 3 min on biofilms alone and in combination. The plates were washed and sonicated to disperse the biofilms, and serial dilutions were carried and aliquots seeded in Sabouraud agar before incubation for 48 h. Next, the colony-forming units per milliliter (CFU/ml; log10) were counted and analyzed statistically (ANOVA, Tukey test, P ≤ 0.05). Results show that S. mutans favors the growth of C. albicans in biofilms with sucrose, with treatment not being effective. However, when the biofilm was grown without sucrose, we found a reduction in biofilm formation and a significant decrease in the PDI treatment (P < 0.0001). In conclusion, both growth and sensitivity to PDI in biofilms of C. albicans are strongly influenced by bacterial combination, and the presence of sucrose affected directly the growth and sensitivity of the biofilm to PDI as sucrose is the substrate for construction of the exopolysaccharide matrix.
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spelling Influence of sucrose on growth and sensitivity of Candida albicans alone and in combination with Enterococcus faecalis and Streptococcus mutans to photodynamic therapyBiofilmCandida albicansEnterococcus faecalisPhotodynamic therapyStreptococcus mutansSucroseThis study has evaluated the effects of photodynamic inactivation (PDI) using erythrosine as photosensitizer and green light-emitting diode (LED) on biofilms of Candida albicans alone and in combination with Enterococcus faecalis and Streptococcus mutans. We have also evaluated the effect of sucrose on biofilm formation and bacterial growth and sensitivity to PDI. Biofilms were formed in suspension of 106 cells/ml on plates before being grown in broth culture with and without sucrose and incubated for 48 h. Next, the treatment was applied using erythrosine at a concentration of 400 μM for 5 min and green LED (532 ± 10 nm) for 3 min on biofilms alone and in combination. The plates were washed and sonicated to disperse the biofilms, and serial dilutions were carried and aliquots seeded in Sabouraud agar before incubation for 48 h. Next, the colony-forming units per milliliter (CFU/ml; log10) were counted and analyzed statistically (ANOVA, Tukey test, P ≤ 0.05). Results show that S. mutans favors the growth of C. albicans in biofilms with sucrose, with treatment not being effective. However, when the biofilm was grown without sucrose, we found a reduction in biofilm formation and a significant decrease in the PDI treatment (P < 0.0001). In conclusion, both growth and sensitivity to PDI in biofilms of C. albicans are strongly influenced by bacterial combination, and the presence of sucrose affected directly the growth and sensitivity of the biofilm to PDI as sucrose is the substrate for construction of the exopolysaccharide matrix.Department of Biosciences and Oral Diagnosis São Paulo State University (Unesp) Institute of Science and Technology, São José dos Campos, Francisco José Longo 777, São DimasDepartment of Biosciences and Oral Diagnosis São Paulo State University (Unesp) Institute of Science and Technology, São José dos Campos, Francisco José Longo 777, São DimasUniversidade Estadual Paulista (Unesp)Tomé, Fernanda Malagutti [UNESP]Paula Ramos, Lucas De [UNESP]Freire, Fernanda [UNESP]Pereira, Cristiane Aparecida [UNESP]de Oliveira, Ingrid Christine Barbosa [UNESP]Junqueira, Juliana Campos [UNESP]Jorge, Antonio Olavo Cardoso [UNESP]Oliveira, Luciane Dias de [UNESP]2018-12-11T17:11:07Z2018-12-11T17:11:07Z2017-08-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1237-1243application/pdfhttp://dx.doi.org/10.1007/s10103-017-2201-2Lasers in Medical Science, v. 32, n. 6, p. 1237-1243, 2017.1435-604X0268-8921http://hdl.handle.net/11449/17443610.1007/s10103-017-2201-22-s2.0-850171356582-s2.0-85017135658.pdf4716676611330311Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengLasers in Medical Science0,713info:eu-repo/semantics/openAccess2024-01-16T06:24:18Zoai:repositorio.unesp.br:11449/174436Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T23:05:33.833931Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Influence of sucrose on growth and sensitivity of Candida albicans alone and in combination with Enterococcus faecalis and Streptococcus mutans to photodynamic therapy
title Influence of sucrose on growth and sensitivity of Candida albicans alone and in combination with Enterococcus faecalis and Streptococcus mutans to photodynamic therapy
spellingShingle Influence of sucrose on growth and sensitivity of Candida albicans alone and in combination with Enterococcus faecalis and Streptococcus mutans to photodynamic therapy
Tomé, Fernanda Malagutti [UNESP]
Biofilm
Candida albicans
Enterococcus faecalis
Photodynamic therapy
Streptococcus mutans
Sucrose
title_short Influence of sucrose on growth and sensitivity of Candida albicans alone and in combination with Enterococcus faecalis and Streptococcus mutans to photodynamic therapy
title_full Influence of sucrose on growth and sensitivity of Candida albicans alone and in combination with Enterococcus faecalis and Streptococcus mutans to photodynamic therapy
title_fullStr Influence of sucrose on growth and sensitivity of Candida albicans alone and in combination with Enterococcus faecalis and Streptococcus mutans to photodynamic therapy
title_full_unstemmed Influence of sucrose on growth and sensitivity of Candida albicans alone and in combination with Enterococcus faecalis and Streptococcus mutans to photodynamic therapy
title_sort Influence of sucrose on growth and sensitivity of Candida albicans alone and in combination with Enterococcus faecalis and Streptococcus mutans to photodynamic therapy
author Tomé, Fernanda Malagutti [UNESP]
author_facet Tomé, Fernanda Malagutti [UNESP]
Paula Ramos, Lucas De [UNESP]
Freire, Fernanda [UNESP]
Pereira, Cristiane Aparecida [UNESP]
de Oliveira, Ingrid Christine Barbosa [UNESP]
Junqueira, Juliana Campos [UNESP]
Jorge, Antonio Olavo Cardoso [UNESP]
Oliveira, Luciane Dias de [UNESP]
author_role author
author2 Paula Ramos, Lucas De [UNESP]
Freire, Fernanda [UNESP]
Pereira, Cristiane Aparecida [UNESP]
de Oliveira, Ingrid Christine Barbosa [UNESP]
Junqueira, Juliana Campos [UNESP]
Jorge, Antonio Olavo Cardoso [UNESP]
Oliveira, Luciane Dias de [UNESP]
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Tomé, Fernanda Malagutti [UNESP]
Paula Ramos, Lucas De [UNESP]
Freire, Fernanda [UNESP]
Pereira, Cristiane Aparecida [UNESP]
de Oliveira, Ingrid Christine Barbosa [UNESP]
Junqueira, Juliana Campos [UNESP]
Jorge, Antonio Olavo Cardoso [UNESP]
Oliveira, Luciane Dias de [UNESP]
dc.subject.por.fl_str_mv Biofilm
Candida albicans
Enterococcus faecalis
Photodynamic therapy
Streptococcus mutans
Sucrose
topic Biofilm
Candida albicans
Enterococcus faecalis
Photodynamic therapy
Streptococcus mutans
Sucrose
description This study has evaluated the effects of photodynamic inactivation (PDI) using erythrosine as photosensitizer and green light-emitting diode (LED) on biofilms of Candida albicans alone and in combination with Enterococcus faecalis and Streptococcus mutans. We have also evaluated the effect of sucrose on biofilm formation and bacterial growth and sensitivity to PDI. Biofilms were formed in suspension of 106 cells/ml on plates before being grown in broth culture with and without sucrose and incubated for 48 h. Next, the treatment was applied using erythrosine at a concentration of 400 μM for 5 min and green LED (532 ± 10 nm) for 3 min on biofilms alone and in combination. The plates were washed and sonicated to disperse the biofilms, and serial dilutions were carried and aliquots seeded in Sabouraud agar before incubation for 48 h. Next, the colony-forming units per milliliter (CFU/ml; log10) were counted and analyzed statistically (ANOVA, Tukey test, P ≤ 0.05). Results show that S. mutans favors the growth of C. albicans in biofilms with sucrose, with treatment not being effective. However, when the biofilm was grown without sucrose, we found a reduction in biofilm formation and a significant decrease in the PDI treatment (P < 0.0001). In conclusion, both growth and sensitivity to PDI in biofilms of C. albicans are strongly influenced by bacterial combination, and the presence of sucrose affected directly the growth and sensitivity of the biofilm to PDI as sucrose is the substrate for construction of the exopolysaccharide matrix.
publishDate 2017
dc.date.none.fl_str_mv 2017-08-01
2018-12-11T17:11:07Z
2018-12-11T17:11:07Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1007/s10103-017-2201-2
Lasers in Medical Science, v. 32, n. 6, p. 1237-1243, 2017.
1435-604X
0268-8921
http://hdl.handle.net/11449/174436
10.1007/s10103-017-2201-2
2-s2.0-85017135658
2-s2.0-85017135658.pdf
4716676611330311
url http://dx.doi.org/10.1007/s10103-017-2201-2
http://hdl.handle.net/11449/174436
identifier_str_mv Lasers in Medical Science, v. 32, n. 6, p. 1237-1243, 2017.
1435-604X
0268-8921
10.1007/s10103-017-2201-2
2-s2.0-85017135658
2-s2.0-85017135658.pdf
4716676611330311
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Lasers in Medical Science
0,713
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 1237-1243
application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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