Influence of sucrose on growth and sensitivity of Candida albicans alone and in combination with Enterococcus faecalis and Streptococcus mutans to photodynamic therapy
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s10103-017-2201-2 http://hdl.handle.net/11449/174436 |
Resumo: | This study has evaluated the effects of photodynamic inactivation (PDI) using erythrosine as photosensitizer and green light-emitting diode (LED) on biofilms of Candida albicans alone and in combination with Enterococcus faecalis and Streptococcus mutans. We have also evaluated the effect of sucrose on biofilm formation and bacterial growth and sensitivity to PDI. Biofilms were formed in suspension of 106 cells/ml on plates before being grown in broth culture with and without sucrose and incubated for 48 h. Next, the treatment was applied using erythrosine at a concentration of 400 μM for 5 min and green LED (532 ± 10 nm) for 3 min on biofilms alone and in combination. The plates were washed and sonicated to disperse the biofilms, and serial dilutions were carried and aliquots seeded in Sabouraud agar before incubation for 48 h. Next, the colony-forming units per milliliter (CFU/ml; log10) were counted and analyzed statistically (ANOVA, Tukey test, P ≤ 0.05). Results show that S. mutans favors the growth of C. albicans in biofilms with sucrose, with treatment not being effective. However, when the biofilm was grown without sucrose, we found a reduction in biofilm formation and a significant decrease in the PDI treatment (P < 0.0001). In conclusion, both growth and sensitivity to PDI in biofilms of C. albicans are strongly influenced by bacterial combination, and the presence of sucrose affected directly the growth and sensitivity of the biofilm to PDI as sucrose is the substrate for construction of the exopolysaccharide matrix. |
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Repositório Institucional da UNESP |
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Influence of sucrose on growth and sensitivity of Candida albicans alone and in combination with Enterococcus faecalis and Streptococcus mutans to photodynamic therapyBiofilmCandida albicansEnterococcus faecalisPhotodynamic therapyStreptococcus mutansSucroseThis study has evaluated the effects of photodynamic inactivation (PDI) using erythrosine as photosensitizer and green light-emitting diode (LED) on biofilms of Candida albicans alone and in combination with Enterococcus faecalis and Streptococcus mutans. We have also evaluated the effect of sucrose on biofilm formation and bacterial growth and sensitivity to PDI. Biofilms were formed in suspension of 106 cells/ml on plates before being grown in broth culture with and without sucrose and incubated for 48 h. Next, the treatment was applied using erythrosine at a concentration of 400 μM for 5 min and green LED (532 ± 10 nm) for 3 min on biofilms alone and in combination. The plates were washed and sonicated to disperse the biofilms, and serial dilutions were carried and aliquots seeded in Sabouraud agar before incubation for 48 h. Next, the colony-forming units per milliliter (CFU/ml; log10) were counted and analyzed statistically (ANOVA, Tukey test, P ≤ 0.05). Results show that S. mutans favors the growth of C. albicans in biofilms with sucrose, with treatment not being effective. However, when the biofilm was grown without sucrose, we found a reduction in biofilm formation and a significant decrease in the PDI treatment (P < 0.0001). In conclusion, both growth and sensitivity to PDI in biofilms of C. albicans are strongly influenced by bacterial combination, and the presence of sucrose affected directly the growth and sensitivity of the biofilm to PDI as sucrose is the substrate for construction of the exopolysaccharide matrix.Department of Biosciences and Oral Diagnosis São Paulo State University (Unesp) Institute of Science and Technology, São José dos Campos, Francisco José Longo 777, São DimasDepartment of Biosciences and Oral Diagnosis São Paulo State University (Unesp) Institute of Science and Technology, São José dos Campos, Francisco José Longo 777, São DimasUniversidade Estadual Paulista (Unesp)Tomé, Fernanda Malagutti [UNESP]Paula Ramos, Lucas De [UNESP]Freire, Fernanda [UNESP]Pereira, Cristiane Aparecida [UNESP]de Oliveira, Ingrid Christine Barbosa [UNESP]Junqueira, Juliana Campos [UNESP]Jorge, Antonio Olavo Cardoso [UNESP]Oliveira, Luciane Dias de [UNESP]2018-12-11T17:11:07Z2018-12-11T17:11:07Z2017-08-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1237-1243application/pdfhttp://dx.doi.org/10.1007/s10103-017-2201-2Lasers in Medical Science, v. 32, n. 6, p. 1237-1243, 2017.1435-604X0268-8921http://hdl.handle.net/11449/17443610.1007/s10103-017-2201-22-s2.0-850171356582-s2.0-85017135658.pdf4716676611330311Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengLasers in Medical Science0,713info:eu-repo/semantics/openAccess2024-01-16T06:24:18Zoai:repositorio.unesp.br:11449/174436Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T23:05:33.833931Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Influence of sucrose on growth and sensitivity of Candida albicans alone and in combination with Enterococcus faecalis and Streptococcus mutans to photodynamic therapy |
title |
Influence of sucrose on growth and sensitivity of Candida albicans alone and in combination with Enterococcus faecalis and Streptococcus mutans to photodynamic therapy |
spellingShingle |
Influence of sucrose on growth and sensitivity of Candida albicans alone and in combination with Enterococcus faecalis and Streptococcus mutans to photodynamic therapy Tomé, Fernanda Malagutti [UNESP] Biofilm Candida albicans Enterococcus faecalis Photodynamic therapy Streptococcus mutans Sucrose |
title_short |
Influence of sucrose on growth and sensitivity of Candida albicans alone and in combination with Enterococcus faecalis and Streptococcus mutans to photodynamic therapy |
title_full |
Influence of sucrose on growth and sensitivity of Candida albicans alone and in combination with Enterococcus faecalis and Streptococcus mutans to photodynamic therapy |
title_fullStr |
Influence of sucrose on growth and sensitivity of Candida albicans alone and in combination with Enterococcus faecalis and Streptococcus mutans to photodynamic therapy |
title_full_unstemmed |
Influence of sucrose on growth and sensitivity of Candida albicans alone and in combination with Enterococcus faecalis and Streptococcus mutans to photodynamic therapy |
title_sort |
Influence of sucrose on growth and sensitivity of Candida albicans alone and in combination with Enterococcus faecalis and Streptococcus mutans to photodynamic therapy |
author |
Tomé, Fernanda Malagutti [UNESP] |
author_facet |
Tomé, Fernanda Malagutti [UNESP] Paula Ramos, Lucas De [UNESP] Freire, Fernanda [UNESP] Pereira, Cristiane Aparecida [UNESP] de Oliveira, Ingrid Christine Barbosa [UNESP] Junqueira, Juliana Campos [UNESP] Jorge, Antonio Olavo Cardoso [UNESP] Oliveira, Luciane Dias de [UNESP] |
author_role |
author |
author2 |
Paula Ramos, Lucas De [UNESP] Freire, Fernanda [UNESP] Pereira, Cristiane Aparecida [UNESP] de Oliveira, Ingrid Christine Barbosa [UNESP] Junqueira, Juliana Campos [UNESP] Jorge, Antonio Olavo Cardoso [UNESP] Oliveira, Luciane Dias de [UNESP] |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Tomé, Fernanda Malagutti [UNESP] Paula Ramos, Lucas De [UNESP] Freire, Fernanda [UNESP] Pereira, Cristiane Aparecida [UNESP] de Oliveira, Ingrid Christine Barbosa [UNESP] Junqueira, Juliana Campos [UNESP] Jorge, Antonio Olavo Cardoso [UNESP] Oliveira, Luciane Dias de [UNESP] |
dc.subject.por.fl_str_mv |
Biofilm Candida albicans Enterococcus faecalis Photodynamic therapy Streptococcus mutans Sucrose |
topic |
Biofilm Candida albicans Enterococcus faecalis Photodynamic therapy Streptococcus mutans Sucrose |
description |
This study has evaluated the effects of photodynamic inactivation (PDI) using erythrosine as photosensitizer and green light-emitting diode (LED) on biofilms of Candida albicans alone and in combination with Enterococcus faecalis and Streptococcus mutans. We have also evaluated the effect of sucrose on biofilm formation and bacterial growth and sensitivity to PDI. Biofilms were formed in suspension of 106 cells/ml on plates before being grown in broth culture with and without sucrose and incubated for 48 h. Next, the treatment was applied using erythrosine at a concentration of 400 μM for 5 min and green LED (532 ± 10 nm) for 3 min on biofilms alone and in combination. The plates were washed and sonicated to disperse the biofilms, and serial dilutions were carried and aliquots seeded in Sabouraud agar before incubation for 48 h. Next, the colony-forming units per milliliter (CFU/ml; log10) were counted and analyzed statistically (ANOVA, Tukey test, P ≤ 0.05). Results show that S. mutans favors the growth of C. albicans in biofilms with sucrose, with treatment not being effective. However, when the biofilm was grown without sucrose, we found a reduction in biofilm formation and a significant decrease in the PDI treatment (P < 0.0001). In conclusion, both growth and sensitivity to PDI in biofilms of C. albicans are strongly influenced by bacterial combination, and the presence of sucrose affected directly the growth and sensitivity of the biofilm to PDI as sucrose is the substrate for construction of the exopolysaccharide matrix. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-08-01 2018-12-11T17:11:07Z 2018-12-11T17:11:07Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s10103-017-2201-2 Lasers in Medical Science, v. 32, n. 6, p. 1237-1243, 2017. 1435-604X 0268-8921 http://hdl.handle.net/11449/174436 10.1007/s10103-017-2201-2 2-s2.0-85017135658 2-s2.0-85017135658.pdf 4716676611330311 |
url |
http://dx.doi.org/10.1007/s10103-017-2201-2 http://hdl.handle.net/11449/174436 |
identifier_str_mv |
Lasers in Medical Science, v. 32, n. 6, p. 1237-1243, 2017. 1435-604X 0268-8921 10.1007/s10103-017-2201-2 2-s2.0-85017135658 2-s2.0-85017135658.pdf 4716676611330311 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Lasers in Medical Science 0,713 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1237-1243 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129489526325248 |