Immune response mediated by Th1 / IL-17 / caspase-9 promotes evolution of periodontal disease
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.archoralbio.2018.09.009 http://hdl.handle.net/11449/185120 |
Resumo: | Introduction: Periodontitis is characterized by inflammatory mediators beyond T lymphocyte function and phenotype (Thl/Th2/Th17). The clinical diversity in periodontitis makes it difficult to characterize the immune response in patients. This study evaluated the profile of the adaptive immune response in the periodontal disease model. Methods: 72 rats (Wistar) were divided into a control group (CTL/day 0) and periodontitis (PD15/15 days and PD60/60 days). In the PD15 and PD60 groups, periodontal disease was induced by ligature with a silk thread placed in the cervical region of the upper first molar. After euthanasia, the periodontal tissue was analyzed by flow cytometry (CD4, CD8, CD25, CD44), semi-quantitative RT-PCR (T-bet, GATA-3, ROR gamma t), semi-quantitative RT-PCR and ELISA IFN-gamma, TNF-alpha, IFN-gamma, IL-4, IL-6, IL-10, IL-17) and by Western blotting (Caspase-9, PCNA). Results: The number of CD4(+) CD25(+), CD4(+) CD44(+), CD8(+) CD25(+) and CD8(+) CD44(+) cells and expression levels of T-bet and GATA-3 are increased in the PD60 group compared to PD15 and CTL. The ROR gamma-t gene transcript increased in the PD15 group in relation to PD60 and CTL. The cytokines IFN-gamma, TNF-alpha and IL-17 increased in the PD60 group in relation to PD15. The expression of Caspase-9 was higher in the PD60 group than in PD15. Conclusions: The results suggest that the evolution of gingivitis to periodontitis is related to the accumulation of activated Thl cells (IFN-gamma and TNF-alpha) associated with the presence of increased IL-17. Studies with inhibitors of these cytokines in periodontal disease may lead to therapy directed at blocking the inflammatory process in this pathology, interrupting bone loss. |
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Immune response mediated by Th1 / IL-17 / caspase-9 promotes evolution of periodontal diseaseCytokinesImmune responsePeriodontitisLymphocytesIntroduction: Periodontitis is characterized by inflammatory mediators beyond T lymphocyte function and phenotype (Thl/Th2/Th17). The clinical diversity in periodontitis makes it difficult to characterize the immune response in patients. This study evaluated the profile of the adaptive immune response in the periodontal disease model. Methods: 72 rats (Wistar) were divided into a control group (CTL/day 0) and periodontitis (PD15/15 days and PD60/60 days). In the PD15 and PD60 groups, periodontal disease was induced by ligature with a silk thread placed in the cervical region of the upper first molar. After euthanasia, the periodontal tissue was analyzed by flow cytometry (CD4, CD8, CD25, CD44), semi-quantitative RT-PCR (T-bet, GATA-3, ROR gamma t), semi-quantitative RT-PCR and ELISA IFN-gamma, TNF-alpha, IFN-gamma, IL-4, IL-6, IL-10, IL-17) and by Western blotting (Caspase-9, PCNA). Results: The number of CD4(+) CD25(+), CD4(+) CD44(+), CD8(+) CD25(+) and CD8(+) CD44(+) cells and expression levels of T-bet and GATA-3 are increased in the PD60 group compared to PD15 and CTL. The ROR gamma-t gene transcript increased in the PD15 group in relation to PD60 and CTL. The cytokines IFN-gamma, TNF-alpha and IL-17 increased in the PD60 group in relation to PD15. The expression of Caspase-9 was higher in the PD60 group than in PD15. Conclusions: The results suggest that the evolution of gingivitis to periodontitis is related to the accumulation of activated Thl cells (IFN-gamma and TNF-alpha) associated with the presence of increased IL-17. Studies with inhibitors of these cytokines in periodontal disease may lead to therapy directed at blocking the inflammatory process in this pathology, interrupting bone loss.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Herminio Ometto FoundationCtr Univ Herminio Ometto UNIARARAS, Grad Program Biomed Sci, Araras, SP, BrazilCtr Univ Herminio Ometto UNIARARAS, Grad Program Odontol, Araras, SP, BrazilSao Paulo State Univ, Dept Diag & Surg, Sch Dent, Araraquara, SP, BrazilUniv Estadual Campinas, Inst Biol, Dept Genet Evolut & Bioagents, Campinas, SP, BrazilSao Paulo State Univ, Dept Diag & Surg, Sch Dent, Araraquara, SP, BrazilFAPESP: 2012-51110-8Elsevier B.V.Ctr Univ Herminio Ometto UNIARARASUniversidade Estadual Paulista (Unesp)Universidade Estadual de Campinas (UNICAMP)Sommer, M. E. L.Dalia, R. A.Nogueira, A. V. B. [UNESP]Cirelli, J. A. [UNESP]Vinolo, M. A. R.Fachi, J. L.Oliveira, C. A.Andrade, T. A. M.Mendonca, F. A. S.Santamaria, M.Felonato, M.2019-10-04T12:32:46Z2019-10-04T12:32:46Z2019-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article77-84http://dx.doi.org/10.1016/j.archoralbio.2018.09.009Archives Of Oral Biology. Oxford: Pergamon-elsevier Science Ltd, v. 97, p. 77-84, 2019.0003-9969http://hdl.handle.net/11449/18512010.1016/j.archoralbio.2018.09.009WOS:000451492700011Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengArchives Of Oral Biologyinfo:eu-repo/semantics/openAccess2024-09-26T15:21:28Zoai:repositorio.unesp.br:11449/185120Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-26T15:21:28Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Immune response mediated by Th1 / IL-17 / caspase-9 promotes evolution of periodontal disease |
title |
Immune response mediated by Th1 / IL-17 / caspase-9 promotes evolution of periodontal disease |
spellingShingle |
Immune response mediated by Th1 / IL-17 / caspase-9 promotes evolution of periodontal disease Sommer, M. E. L. Cytokines Immune response Periodontitis Lymphocytes |
title_short |
Immune response mediated by Th1 / IL-17 / caspase-9 promotes evolution of periodontal disease |
title_full |
Immune response mediated by Th1 / IL-17 / caspase-9 promotes evolution of periodontal disease |
title_fullStr |
Immune response mediated by Th1 / IL-17 / caspase-9 promotes evolution of periodontal disease |
title_full_unstemmed |
Immune response mediated by Th1 / IL-17 / caspase-9 promotes evolution of periodontal disease |
title_sort |
Immune response mediated by Th1 / IL-17 / caspase-9 promotes evolution of periodontal disease |
author |
Sommer, M. E. L. |
author_facet |
Sommer, M. E. L. Dalia, R. A. Nogueira, A. V. B. [UNESP] Cirelli, J. A. [UNESP] Vinolo, M. A. R. Fachi, J. L. Oliveira, C. A. Andrade, T. A. M. Mendonca, F. A. S. Santamaria, M. Felonato, M. |
author_role |
author |
author2 |
Dalia, R. A. Nogueira, A. V. B. [UNESP] Cirelli, J. A. [UNESP] Vinolo, M. A. R. Fachi, J. L. Oliveira, C. A. Andrade, T. A. M. Mendonca, F. A. S. Santamaria, M. Felonato, M. |
author2_role |
author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Ctr Univ Herminio Ometto UNIARARAS Universidade Estadual Paulista (Unesp) Universidade Estadual de Campinas (UNICAMP) |
dc.contributor.author.fl_str_mv |
Sommer, M. E. L. Dalia, R. A. Nogueira, A. V. B. [UNESP] Cirelli, J. A. [UNESP] Vinolo, M. A. R. Fachi, J. L. Oliveira, C. A. Andrade, T. A. M. Mendonca, F. A. S. Santamaria, M. Felonato, M. |
dc.subject.por.fl_str_mv |
Cytokines Immune response Periodontitis Lymphocytes |
topic |
Cytokines Immune response Periodontitis Lymphocytes |
description |
Introduction: Periodontitis is characterized by inflammatory mediators beyond T lymphocyte function and phenotype (Thl/Th2/Th17). The clinical diversity in periodontitis makes it difficult to characterize the immune response in patients. This study evaluated the profile of the adaptive immune response in the periodontal disease model. Methods: 72 rats (Wistar) were divided into a control group (CTL/day 0) and periodontitis (PD15/15 days and PD60/60 days). In the PD15 and PD60 groups, periodontal disease was induced by ligature with a silk thread placed in the cervical region of the upper first molar. After euthanasia, the periodontal tissue was analyzed by flow cytometry (CD4, CD8, CD25, CD44), semi-quantitative RT-PCR (T-bet, GATA-3, ROR gamma t), semi-quantitative RT-PCR and ELISA IFN-gamma, TNF-alpha, IFN-gamma, IL-4, IL-6, IL-10, IL-17) and by Western blotting (Caspase-9, PCNA). Results: The number of CD4(+) CD25(+), CD4(+) CD44(+), CD8(+) CD25(+) and CD8(+) CD44(+) cells and expression levels of T-bet and GATA-3 are increased in the PD60 group compared to PD15 and CTL. The ROR gamma-t gene transcript increased in the PD15 group in relation to PD60 and CTL. The cytokines IFN-gamma, TNF-alpha and IL-17 increased in the PD60 group in relation to PD15. The expression of Caspase-9 was higher in the PD60 group than in PD15. Conclusions: The results suggest that the evolution of gingivitis to periodontitis is related to the accumulation of activated Thl cells (IFN-gamma and TNF-alpha) associated with the presence of increased IL-17. Studies with inhibitors of these cytokines in periodontal disease may lead to therapy directed at blocking the inflammatory process in this pathology, interrupting bone loss. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-10-04T12:32:46Z 2019-10-04T12:32:46Z 2019-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.archoralbio.2018.09.009 Archives Of Oral Biology. Oxford: Pergamon-elsevier Science Ltd, v. 97, p. 77-84, 2019. 0003-9969 http://hdl.handle.net/11449/185120 10.1016/j.archoralbio.2018.09.009 WOS:000451492700011 |
url |
http://dx.doi.org/10.1016/j.archoralbio.2018.09.009 http://hdl.handle.net/11449/185120 |
identifier_str_mv |
Archives Of Oral Biology. Oxford: Pergamon-elsevier Science Ltd, v. 97, p. 77-84, 2019. 0003-9969 10.1016/j.archoralbio.2018.09.009 WOS:000451492700011 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Archives Of Oral Biology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
77-84 |
dc.publisher.none.fl_str_mv |
Elsevier B.V. |
publisher.none.fl_str_mv |
Elsevier B.V. |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
repositoriounesp@unesp.br |
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1813546428762423296 |