Immune response mediated by Th1 / IL-17 / caspase-9 promotes evolution of periodontal disease

Detalhes bibliográficos
Autor(a) principal: Sommer, M. E. L.
Data de Publicação: 2019
Outros Autores: Dalia, R. A., Nogueira, A. V. B. [UNESP], Cirelli, J. A. [UNESP], Vinolo, M. A. R., Fachi, J. L., Oliveira, C. A., Andrade, T. A. M., Mendonca, F. A. S., Santamaria, M., Felonato, M.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.archoralbio.2018.09.009
http://hdl.handle.net/11449/185120
Resumo: Introduction: Periodontitis is characterized by inflammatory mediators beyond T lymphocyte function and phenotype (Thl/Th2/Th17). The clinical diversity in periodontitis makes it difficult to characterize the immune response in patients. This study evaluated the profile of the adaptive immune response in the periodontal disease model. Methods: 72 rats (Wistar) were divided into a control group (CTL/day 0) and periodontitis (PD15/15 days and PD60/60 days). In the PD15 and PD60 groups, periodontal disease was induced by ligature with a silk thread placed in the cervical region of the upper first molar. After euthanasia, the periodontal tissue was analyzed by flow cytometry (CD4, CD8, CD25, CD44), semi-quantitative RT-PCR (T-bet, GATA-3, ROR gamma t), semi-quantitative RT-PCR and ELISA IFN-gamma, TNF-alpha, IFN-gamma, IL-4, IL-6, IL-10, IL-17) and by Western blotting (Caspase-9, PCNA). Results: The number of CD4(+) CD25(+), CD4(+) CD44(+), CD8(+) CD25(+) and CD8(+) CD44(+) cells and expression levels of T-bet and GATA-3 are increased in the PD60 group compared to PD15 and CTL. The ROR gamma-t gene transcript increased in the PD15 group in relation to PD60 and CTL. The cytokines IFN-gamma, TNF-alpha and IL-17 increased in the PD60 group in relation to PD15. The expression of Caspase-9 was higher in the PD60 group than in PD15. Conclusions: The results suggest that the evolution of gingivitis to periodontitis is related to the accumulation of activated Thl cells (IFN-gamma and TNF-alpha) associated with the presence of increased IL-17. Studies with inhibitors of these cytokines in periodontal disease may lead to therapy directed at blocking the inflammatory process in this pathology, interrupting bone loss.
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spelling Immune response mediated by Th1 / IL-17 / caspase-9 promotes evolution of periodontal diseaseCytokinesImmune responsePeriodontitisLymphocytesIntroduction: Periodontitis is characterized by inflammatory mediators beyond T lymphocyte function and phenotype (Thl/Th2/Th17). The clinical diversity in periodontitis makes it difficult to characterize the immune response in patients. This study evaluated the profile of the adaptive immune response in the periodontal disease model. Methods: 72 rats (Wistar) were divided into a control group (CTL/day 0) and periodontitis (PD15/15 days and PD60/60 days). In the PD15 and PD60 groups, periodontal disease was induced by ligature with a silk thread placed in the cervical region of the upper first molar. After euthanasia, the periodontal tissue was analyzed by flow cytometry (CD4, CD8, CD25, CD44), semi-quantitative RT-PCR (T-bet, GATA-3, ROR gamma t), semi-quantitative RT-PCR and ELISA IFN-gamma, TNF-alpha, IFN-gamma, IL-4, IL-6, IL-10, IL-17) and by Western blotting (Caspase-9, PCNA). Results: The number of CD4(+) CD25(+), CD4(+) CD44(+), CD8(+) CD25(+) and CD8(+) CD44(+) cells and expression levels of T-bet and GATA-3 are increased in the PD60 group compared to PD15 and CTL. The ROR gamma-t gene transcript increased in the PD15 group in relation to PD60 and CTL. The cytokines IFN-gamma, TNF-alpha and IL-17 increased in the PD60 group in relation to PD15. The expression of Caspase-9 was higher in the PD60 group than in PD15. Conclusions: The results suggest that the evolution of gingivitis to periodontitis is related to the accumulation of activated Thl cells (IFN-gamma and TNF-alpha) associated with the presence of increased IL-17. Studies with inhibitors of these cytokines in periodontal disease may lead to therapy directed at blocking the inflammatory process in this pathology, interrupting bone loss.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Herminio Ometto FoundationCtr Univ Herminio Ometto UNIARARAS, Grad Program Biomed Sci, Araras, SP, BrazilCtr Univ Herminio Ometto UNIARARAS, Grad Program Odontol, Araras, SP, BrazilSao Paulo State Univ, Dept Diag & Surg, Sch Dent, Araraquara, SP, BrazilUniv Estadual Campinas, Inst Biol, Dept Genet Evolut & Bioagents, Campinas, SP, BrazilSao Paulo State Univ, Dept Diag & Surg, Sch Dent, Araraquara, SP, BrazilFAPESP: 2012-51110-8Elsevier B.V.Ctr Univ Herminio Ometto UNIARARASUniversidade Estadual Paulista (Unesp)Universidade Estadual de Campinas (UNICAMP)Sommer, M. E. L.Dalia, R. A.Nogueira, A. V. B. [UNESP]Cirelli, J. A. [UNESP]Vinolo, M. A. R.Fachi, J. L.Oliveira, C. A.Andrade, T. A. M.Mendonca, F. A. S.Santamaria, M.Felonato, M.2019-10-04T12:32:46Z2019-10-04T12:32:46Z2019-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article77-84http://dx.doi.org/10.1016/j.archoralbio.2018.09.009Archives Of Oral Biology. Oxford: Pergamon-elsevier Science Ltd, v. 97, p. 77-84, 2019.0003-9969http://hdl.handle.net/11449/18512010.1016/j.archoralbio.2018.09.009WOS:000451492700011Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengArchives Of Oral Biologyinfo:eu-repo/semantics/openAccess2024-09-26T15:21:28Zoai:repositorio.unesp.br:11449/185120Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-26T15:21:28Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Immune response mediated by Th1 / IL-17 / caspase-9 promotes evolution of periodontal disease
title Immune response mediated by Th1 / IL-17 / caspase-9 promotes evolution of periodontal disease
spellingShingle Immune response mediated by Th1 / IL-17 / caspase-9 promotes evolution of periodontal disease
Sommer, M. E. L.
Cytokines
Immune response
Periodontitis
Lymphocytes
title_short Immune response mediated by Th1 / IL-17 / caspase-9 promotes evolution of periodontal disease
title_full Immune response mediated by Th1 / IL-17 / caspase-9 promotes evolution of periodontal disease
title_fullStr Immune response mediated by Th1 / IL-17 / caspase-9 promotes evolution of periodontal disease
title_full_unstemmed Immune response mediated by Th1 / IL-17 / caspase-9 promotes evolution of periodontal disease
title_sort Immune response mediated by Th1 / IL-17 / caspase-9 promotes evolution of periodontal disease
author Sommer, M. E. L.
author_facet Sommer, M. E. L.
Dalia, R. A.
Nogueira, A. V. B. [UNESP]
Cirelli, J. A. [UNESP]
Vinolo, M. A. R.
Fachi, J. L.
Oliveira, C. A.
Andrade, T. A. M.
Mendonca, F. A. S.
Santamaria, M.
Felonato, M.
author_role author
author2 Dalia, R. A.
Nogueira, A. V. B. [UNESP]
Cirelli, J. A. [UNESP]
Vinolo, M. A. R.
Fachi, J. L.
Oliveira, C. A.
Andrade, T. A. M.
Mendonca, F. A. S.
Santamaria, M.
Felonato, M.
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ctr Univ Herminio Ometto UNIARARAS
Universidade Estadual Paulista (Unesp)
Universidade Estadual de Campinas (UNICAMP)
dc.contributor.author.fl_str_mv Sommer, M. E. L.
Dalia, R. A.
Nogueira, A. V. B. [UNESP]
Cirelli, J. A. [UNESP]
Vinolo, M. A. R.
Fachi, J. L.
Oliveira, C. A.
Andrade, T. A. M.
Mendonca, F. A. S.
Santamaria, M.
Felonato, M.
dc.subject.por.fl_str_mv Cytokines
Immune response
Periodontitis
Lymphocytes
topic Cytokines
Immune response
Periodontitis
Lymphocytes
description Introduction: Periodontitis is characterized by inflammatory mediators beyond T lymphocyte function and phenotype (Thl/Th2/Th17). The clinical diversity in periodontitis makes it difficult to characterize the immune response in patients. This study evaluated the profile of the adaptive immune response in the periodontal disease model. Methods: 72 rats (Wistar) were divided into a control group (CTL/day 0) and periodontitis (PD15/15 days and PD60/60 days). In the PD15 and PD60 groups, periodontal disease was induced by ligature with a silk thread placed in the cervical region of the upper first molar. After euthanasia, the periodontal tissue was analyzed by flow cytometry (CD4, CD8, CD25, CD44), semi-quantitative RT-PCR (T-bet, GATA-3, ROR gamma t), semi-quantitative RT-PCR and ELISA IFN-gamma, TNF-alpha, IFN-gamma, IL-4, IL-6, IL-10, IL-17) and by Western blotting (Caspase-9, PCNA). Results: The number of CD4(+) CD25(+), CD4(+) CD44(+), CD8(+) CD25(+) and CD8(+) CD44(+) cells and expression levels of T-bet and GATA-3 are increased in the PD60 group compared to PD15 and CTL. The ROR gamma-t gene transcript increased in the PD15 group in relation to PD60 and CTL. The cytokines IFN-gamma, TNF-alpha and IL-17 increased in the PD60 group in relation to PD15. The expression of Caspase-9 was higher in the PD60 group than in PD15. Conclusions: The results suggest that the evolution of gingivitis to periodontitis is related to the accumulation of activated Thl cells (IFN-gamma and TNF-alpha) associated with the presence of increased IL-17. Studies with inhibitors of these cytokines in periodontal disease may lead to therapy directed at blocking the inflammatory process in this pathology, interrupting bone loss.
publishDate 2019
dc.date.none.fl_str_mv 2019-10-04T12:32:46Z
2019-10-04T12:32:46Z
2019-01-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.archoralbio.2018.09.009
Archives Of Oral Biology. Oxford: Pergamon-elsevier Science Ltd, v. 97, p. 77-84, 2019.
0003-9969
http://hdl.handle.net/11449/185120
10.1016/j.archoralbio.2018.09.009
WOS:000451492700011
url http://dx.doi.org/10.1016/j.archoralbio.2018.09.009
http://hdl.handle.net/11449/185120
identifier_str_mv Archives Of Oral Biology. Oxford: Pergamon-elsevier Science Ltd, v. 97, p. 77-84, 2019.
0003-9969
10.1016/j.archoralbio.2018.09.009
WOS:000451492700011
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Archives Of Oral Biology
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 77-84
dc.publisher.none.fl_str_mv Elsevier B.V.
publisher.none.fl_str_mv Elsevier B.V.
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv repositoriounesp@unesp.br
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