Comparative study of bone tissue accelerated regeneration by latex membranes from Hevea brasiliensis and Hancornia speciosa

Detalhes bibliográficos
Autor(a) principal: Floriano, Juliana Ferreira [UNESP]
Data de Publicação: 2016
Outros Autores: Neto, Fausto Capuano [UNESP], Lima Silveira da Mota, Ligia Souza [UNESP], Furtado, Edson Luiz [UNESP], Ferreira, Rui Seabra [UNESP], Barraviera, Benedito [UNESP], Goncalves, Pablo Jose, Almeida, Luciane Madureira de, Borges, Felipe Azevedo [UNESP], Herculano, Rondinelli Donizetti [UNESP], Oliveira Graeff, Carlos Frederico de [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1088/2057-1976/2/4/045007
http://hdl.handle.net/11449/163038
Resumo: Bone loss is a common problem after accidental traumas, cancers, congenital defects, and surgical procedures. The techniques normally used in large bone restoration involve complex and invasive procedures such as grafting. Thus, it is of interest to develop alternatives such as bioactive materials to induce accelerated bone regeneration. Natural rubber (NR) membranes are potential candidates due to their characteristics such as biocompatibility, angiogenic potential, flexibility, mechanical stability, surface porosity, and permeability. The present study aims at assessing the osteogenic potential ofNR membranes of clones of high bioactivity of Hevea brasiliensis (RRIM 600 and IAN 873) and of Hancornia speciosa, as well as Physicochemical characterization at theNRmembranes by scanning electron microscopy, Fourier transform infrared spectroscopy, and tensile tests. Critical-size bone defects were surgically made in adult male rabbit calvarium. Afibrin sealant (FS) was used to fix the membranes as a replacement for cyanoacrylate. Wecompared the respective osteogenic potentials of the tested membranes against a control group in healthy animals. The new bone formed was characterized using radiography, x-ray tomography, and histological and morphometric studies. Our results show that both membranes have great potential for regenerating bone tissue, with higher bioactivity compared to the gold standard (PTFE), which was used as positive control. In bothNR membranes the stress-strain profile shows low stress at small strain, characteristic of elastomer with a low degree of reticulation, followed by an increase in the stress at high deformation and two main differences between both NRL biomembranes, related to its composition. The FS acted satisfactorily in the tests, being highly recommended as a substitute for cyanoacrylate in this type of application.
id UNSP_ec74bdca5e5d259f7dc638aa02785c26
oai_identifier_str oai:repositorio.unesp.br:11449/163038
network_acronym_str UNSP
network_name_str Repositório Institucional da UNESP
repository_id_str 2946
spelling Comparative study of bone tissue accelerated regeneration by latex membranes from Hevea brasiliensis and Hancornia speciosaHevea brasiliensisHancornia speciosamembranebioactivitybone regenerationBone loss is a common problem after accidental traumas, cancers, congenital defects, and surgical procedures. The techniques normally used in large bone restoration involve complex and invasive procedures such as grafting. Thus, it is of interest to develop alternatives such as bioactive materials to induce accelerated bone regeneration. Natural rubber (NR) membranes are potential candidates due to their characteristics such as biocompatibility, angiogenic potential, flexibility, mechanical stability, surface porosity, and permeability. The present study aims at assessing the osteogenic potential ofNR membranes of clones of high bioactivity of Hevea brasiliensis (RRIM 600 and IAN 873) and of Hancornia speciosa, as well as Physicochemical characterization at theNRmembranes by scanning electron microscopy, Fourier transform infrared spectroscopy, and tensile tests. Critical-size bone defects were surgically made in adult male rabbit calvarium. Afibrin sealant (FS) was used to fix the membranes as a replacement for cyanoacrylate. Wecompared the respective osteogenic potentials of the tested membranes against a control group in healthy animals. The new bone formed was characterized using radiography, x-ray tomography, and histological and morphometric studies. Our results show that both membranes have great potential for regenerating bone tissue, with higher bioactivity compared to the gold standard (PTFE), which was used as positive control. In bothNR membranes the stress-strain profile shows low stress at small strain, characteristic of elastomer with a low degree of reticulation, followed by an increase in the stress at high deformation and two main differences between both NRL biomembranes, related to its composition. The FS acted satisfactorily in the tests, being highly recommended as a substitute for cyanoacrylate in this type of application.UEGCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Julio de Mesquita Filho State Univ Sao Paulo, Dept Phys, Bauru, SP, BrazilJulio de Mesquita Filho State Univ Sao Paulo, Dept Trop Dis, Botucatu, SP, BrazilJulio de Mesquita Filho State Univ Sao Paulo, Dept Genet, Botucatu, SP, BrazilJulio de Mesquita Filho State Univ Sao Paulo, Dept Plant Protect, Botucatu, SP, BrazilJulio de Mesquita Filho State Univ Sao Paulo, Fac Med, Dept Trop Dis & Diagnost Imaging, Botucatu, SP, BrazilJulio de Mesquita Filho State Univ Sao Paulo, Ctr Study Venoms & Venomous Anim CEVAP, Botucatu, SP, BrazilUniv Fed Goias, Inst Phys, Goiania, Go, BrazilState Univ Goias, Ipameri, Go, BrazilJulio de Mesquita Filho State Univ Sao Paulo, Inst Chem, Biomat Grp, Araraquara, SP, BrazilJulio de Mesquita Filho State Univ Sao Paulo, Dept Phys, Bauru, SP, BrazilJulio de Mesquita Filho State Univ Sao Paulo, Dept Trop Dis, Botucatu, SP, BrazilJulio de Mesquita Filho State Univ Sao Paulo, Dept Genet, Botucatu, SP, BrazilJulio de Mesquita Filho State Univ Sao Paulo, Dept Plant Protect, Botucatu, SP, BrazilJulio de Mesquita Filho State Univ Sao Paulo, Fac Med, Dept Trop Dis & Diagnost Imaging, Botucatu, SP, BrazilJulio de Mesquita Filho State Univ Sao Paulo, Ctr Study Venoms & Venomous Anim CEVAP, Botucatu, SP, BrazilJulio de Mesquita Filho State Univ Sao Paulo, Inst Chem, Biomat Grp, Araraquara, SP, BrazilIop Publishing LtdUniversidade Estadual Paulista (Unesp)Universidade Federal de Goiás (UFG)State Univ GoiasFloriano, Juliana Ferreira [UNESP]Neto, Fausto Capuano [UNESP]Lima Silveira da Mota, Ligia Souza [UNESP]Furtado, Edson Luiz [UNESP]Ferreira, Rui Seabra [UNESP]Barraviera, Benedito [UNESP]Goncalves, Pablo JoseAlmeida, Luciane Madureira deBorges, Felipe Azevedo [UNESP]Herculano, Rondinelli Donizetti [UNESP]Oliveira Graeff, Carlos Frederico de [UNESP]2018-11-26T17:39:51Z2018-11-26T17:39:51Z2016-08-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article15application/pdfhttp://dx.doi.org/10.1088/2057-1976/2/4/045007Biomedical Physics & Engineering Express. Bristol: Iop Publishing Ltd, v. 2, n. 4, 15 p., 2016.2057-1976http://hdl.handle.net/11449/16303810.1088/2057-1976/2/4/045007WOS:000406024400007WOS000406024400007.pdf38459894858333950000-0002-6924-835XWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBiomedical Physics & Engineering Express0,196info:eu-repo/semantics/openAccess2024-08-15T15:22:35Zoai:repositorio.unesp.br:11449/163038Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-15T15:22:35Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Comparative study of bone tissue accelerated regeneration by latex membranes from Hevea brasiliensis and Hancornia speciosa
title Comparative study of bone tissue accelerated regeneration by latex membranes from Hevea brasiliensis and Hancornia speciosa
spellingShingle Comparative study of bone tissue accelerated regeneration by latex membranes from Hevea brasiliensis and Hancornia speciosa
Floriano, Juliana Ferreira [UNESP]
Hevea brasiliensis
Hancornia speciosa
membrane
bioactivity
bone regeneration
title_short Comparative study of bone tissue accelerated regeneration by latex membranes from Hevea brasiliensis and Hancornia speciosa
title_full Comparative study of bone tissue accelerated regeneration by latex membranes from Hevea brasiliensis and Hancornia speciosa
title_fullStr Comparative study of bone tissue accelerated regeneration by latex membranes from Hevea brasiliensis and Hancornia speciosa
title_full_unstemmed Comparative study of bone tissue accelerated regeneration by latex membranes from Hevea brasiliensis and Hancornia speciosa
title_sort Comparative study of bone tissue accelerated regeneration by latex membranes from Hevea brasiliensis and Hancornia speciosa
author Floriano, Juliana Ferreira [UNESP]
author_facet Floriano, Juliana Ferreira [UNESP]
Neto, Fausto Capuano [UNESP]
Lima Silveira da Mota, Ligia Souza [UNESP]
Furtado, Edson Luiz [UNESP]
Ferreira, Rui Seabra [UNESP]
Barraviera, Benedito [UNESP]
Goncalves, Pablo Jose
Almeida, Luciane Madureira de
Borges, Felipe Azevedo [UNESP]
Herculano, Rondinelli Donizetti [UNESP]
Oliveira Graeff, Carlos Frederico de [UNESP]
author_role author
author2 Neto, Fausto Capuano [UNESP]
Lima Silveira da Mota, Ligia Souza [UNESP]
Furtado, Edson Luiz [UNESP]
Ferreira, Rui Seabra [UNESP]
Barraviera, Benedito [UNESP]
Goncalves, Pablo Jose
Almeida, Luciane Madureira de
Borges, Felipe Azevedo [UNESP]
Herculano, Rondinelli Donizetti [UNESP]
Oliveira Graeff, Carlos Frederico de [UNESP]
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Universidade Federal de Goiás (UFG)
State Univ Goias
dc.contributor.author.fl_str_mv Floriano, Juliana Ferreira [UNESP]
Neto, Fausto Capuano [UNESP]
Lima Silveira da Mota, Ligia Souza [UNESP]
Furtado, Edson Luiz [UNESP]
Ferreira, Rui Seabra [UNESP]
Barraviera, Benedito [UNESP]
Goncalves, Pablo Jose
Almeida, Luciane Madureira de
Borges, Felipe Azevedo [UNESP]
Herculano, Rondinelli Donizetti [UNESP]
Oliveira Graeff, Carlos Frederico de [UNESP]
dc.subject.por.fl_str_mv Hevea brasiliensis
Hancornia speciosa
membrane
bioactivity
bone regeneration
topic Hevea brasiliensis
Hancornia speciosa
membrane
bioactivity
bone regeneration
description Bone loss is a common problem after accidental traumas, cancers, congenital defects, and surgical procedures. The techniques normally used in large bone restoration involve complex and invasive procedures such as grafting. Thus, it is of interest to develop alternatives such as bioactive materials to induce accelerated bone regeneration. Natural rubber (NR) membranes are potential candidates due to their characteristics such as biocompatibility, angiogenic potential, flexibility, mechanical stability, surface porosity, and permeability. The present study aims at assessing the osteogenic potential ofNR membranes of clones of high bioactivity of Hevea brasiliensis (RRIM 600 and IAN 873) and of Hancornia speciosa, as well as Physicochemical characterization at theNRmembranes by scanning electron microscopy, Fourier transform infrared spectroscopy, and tensile tests. Critical-size bone defects were surgically made in adult male rabbit calvarium. Afibrin sealant (FS) was used to fix the membranes as a replacement for cyanoacrylate. Wecompared the respective osteogenic potentials of the tested membranes against a control group in healthy animals. The new bone formed was characterized using radiography, x-ray tomography, and histological and morphometric studies. Our results show that both membranes have great potential for regenerating bone tissue, with higher bioactivity compared to the gold standard (PTFE), which was used as positive control. In bothNR membranes the stress-strain profile shows low stress at small strain, characteristic of elastomer with a low degree of reticulation, followed by an increase in the stress at high deformation and two main differences between both NRL biomembranes, related to its composition. The FS acted satisfactorily in the tests, being highly recommended as a substitute for cyanoacrylate in this type of application.
publishDate 2016
dc.date.none.fl_str_mv 2016-08-01
2018-11-26T17:39:51Z
2018-11-26T17:39:51Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1088/2057-1976/2/4/045007
Biomedical Physics & Engineering Express. Bristol: Iop Publishing Ltd, v. 2, n. 4, 15 p., 2016.
2057-1976
http://hdl.handle.net/11449/163038
10.1088/2057-1976/2/4/045007
WOS:000406024400007
WOS000406024400007.pdf
3845989485833395
0000-0002-6924-835X
url http://dx.doi.org/10.1088/2057-1976/2/4/045007
http://hdl.handle.net/11449/163038
identifier_str_mv Biomedical Physics & Engineering Express. Bristol: Iop Publishing Ltd, v. 2, n. 4, 15 p., 2016.
2057-1976
10.1088/2057-1976/2/4/045007
WOS:000406024400007
WOS000406024400007.pdf
3845989485833395
0000-0002-6924-835X
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Biomedical Physics & Engineering Express
0,196
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 15
application/pdf
dc.publisher.none.fl_str_mv Iop Publishing Ltd
publisher.none.fl_str_mv Iop Publishing Ltd
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1808128127933612032