The effects of storage time and temperature on the stability of salivary phosphatases, transaminases and dehydrogenase

Detalhes bibliográficos
Autor(a) principal: dos Santos, Damaris Raissa [UNESP]
Data de Publicação: 2018
Outros Autores: Souza, Rayne Oliveira [UNESP], Dias, Layani Bertaglia [UNESP], Ribas, Tayná Buffulin [UNESP], de Oliveira, Luis Cezar Farias, Sumida, Doris Hissako [UNESP], Dornelles, Rita Cássia Menegati [UNESP], Nakamune, Ana Cláudia de Melo Stevanato [UNESP], Chaves-Neto, Antonio Hernandes [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.archoralbio.2017.10.016
http://hdl.handle.net/11449/175378
Resumo: Objectives To investigate the influence of temperature and storage time on salivary acid phosphatase (ACP), tartrate-resistant acid phosphatase (TRAP), alkaline phosphatase (ALP), aspartate aminotransferase (AST) and alanine aminotransferase (ALT) and lactate dehydrogenase (LDH). Design Unstimulated whole expectorated saliva was collected from healthy men and women subjects (n = 26) between 8 and 10 a.m. The saliva samples were centrifuged, and the supernatants were measured for ACP, TRAP, ALP, AST, ALT and LDH activities immediately (without freezing) [baseline values] and after time intervals of 3, 7, 14 and 28 days (d) of storage at −20 °C and −80 °C using spectrophotometric methods The influence of storage time was analyzed by one-way ANOVA followed by the Dunnett post-test, while the paired Student's-t-test was used to compare the differences between the temperature (p < 0.05). Results There was significant decline in the activities of all enzymes at −20 °C with increasing storage time. This decrease was relevant from day 14 onward for the majority of the enzymes, with the exception of AST. After day 28, the more sensitive enzymes were ALP and LDH, which showed residual activity of 39% and 16%, respectively, compared with baseline values. There were considerable, but insignificant changes, in the activities of all enzymes after storage at −80 °C for 28 days. Conclusions Frozen samples should be kept at −80 °C to preserve these activities, but there are restrictions for the enzymes ALP, ALT and LDH. Storage of samples at −20 °C could introduce high error variance in measured activities.
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spelling The effects of storage time and temperature on the stability of salivary phosphatases, transaminases and dehydrogenaseDehydrogenasePhosphatasesSalivaStorageTemperatureTransaminasesObjectives To investigate the influence of temperature and storage time on salivary acid phosphatase (ACP), tartrate-resistant acid phosphatase (TRAP), alkaline phosphatase (ALP), aspartate aminotransferase (AST) and alanine aminotransferase (ALT) and lactate dehydrogenase (LDH). Design Unstimulated whole expectorated saliva was collected from healthy men and women subjects (n = 26) between 8 and 10 a.m. The saliva samples were centrifuged, and the supernatants were measured for ACP, TRAP, ALP, AST, ALT and LDH activities immediately (without freezing) [baseline values] and after time intervals of 3, 7, 14 and 28 days (d) of storage at −20 °C and −80 °C using spectrophotometric methods The influence of storage time was analyzed by one-way ANOVA followed by the Dunnett post-test, while the paired Student's-t-test was used to compare the differences between the temperature (p < 0.05). Results There was significant decline in the activities of all enzymes at −20 °C with increasing storage time. This decrease was relevant from day 14 onward for the majority of the enzymes, with the exception of AST. After day 28, the more sensitive enzymes were ALP and LDH, which showed residual activity of 39% and 16%, respectively, compared with baseline values. There were considerable, but insignificant changes, in the activities of all enzymes after storage at −80 °C for 28 days. Conclusions Frozen samples should be kept at −80 °C to preserve these activities, but there are restrictions for the enzymes ALP, ALT and LDH. Storage of samples at −20 °C could introduce high error variance in measured activities.Paulista University (UNIP), Araçatuba, Avenida Baguaçu, 1939Department of Basic Sciences São Paulo State University (Unesp) School of Dentistry, Araçatuba, Rodovia Marechal Rondon 527/528 km, Campus UniversitárioPrograma Multicêntrico de Pós-Graduação em Ciências Fisiológicas – SBFis São Paulo State University (Unesp) School of Dentistry, Rodovia Marechal Rondon 527/528 km, Campus UniversitárioDepartment of Basic Sciences São Paulo State University (Unesp) School of Dentistry, Araçatuba, Rodovia Marechal Rondon 527/528 km, Campus UniversitárioPrograma Multicêntrico de Pós-Graduação em Ciências Fisiológicas – SBFis São Paulo State University (Unesp) School of Dentistry, Rodovia Marechal Rondon 527/528 km, Campus UniversitárioPaulista University (UNIP)Universidade Estadual Paulista (Unesp)dos Santos, Damaris Raissa [UNESP]Souza, Rayne Oliveira [UNESP]Dias, Layani Bertaglia [UNESP]Ribas, Tayná Buffulin [UNESP]de Oliveira, Luis Cezar FariasSumida, Doris Hissako [UNESP]Dornelles, Rita Cássia Menegati [UNESP]Nakamune, Ana Cláudia de Melo Stevanato [UNESP]Chaves-Neto, Antonio Hernandes [UNESP]2018-12-11T17:15:34Z2018-12-11T17:15:34Z2018-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article160-165application/pdfhttp://dx.doi.org/10.1016/j.archoralbio.2017.10.016Archives of Oral Biology, v. 85, p. 160-165.1879-15060003-9969http://hdl.handle.net/11449/17537810.1016/j.archoralbio.2017.10.0162-s2.0-850321734102-s2.0-85032173410.pdf54359024227848890000-0003-0783-6612Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengArchives of Oral Biology0,752info:eu-repo/semantics/openAccess2024-09-19T14:02:45Zoai:repositorio.unesp.br:11449/175378Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-19T14:02:45Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv The effects of storage time and temperature on the stability of salivary phosphatases, transaminases and dehydrogenase
title The effects of storage time and temperature on the stability of salivary phosphatases, transaminases and dehydrogenase
spellingShingle The effects of storage time and temperature on the stability of salivary phosphatases, transaminases and dehydrogenase
dos Santos, Damaris Raissa [UNESP]
Dehydrogenase
Phosphatases
Saliva
Storage
Temperature
Transaminases
title_short The effects of storage time and temperature on the stability of salivary phosphatases, transaminases and dehydrogenase
title_full The effects of storage time and temperature on the stability of salivary phosphatases, transaminases and dehydrogenase
title_fullStr The effects of storage time and temperature on the stability of salivary phosphatases, transaminases and dehydrogenase
title_full_unstemmed The effects of storage time and temperature on the stability of salivary phosphatases, transaminases and dehydrogenase
title_sort The effects of storage time and temperature on the stability of salivary phosphatases, transaminases and dehydrogenase
author dos Santos, Damaris Raissa [UNESP]
author_facet dos Santos, Damaris Raissa [UNESP]
Souza, Rayne Oliveira [UNESP]
Dias, Layani Bertaglia [UNESP]
Ribas, Tayná Buffulin [UNESP]
de Oliveira, Luis Cezar Farias
Sumida, Doris Hissako [UNESP]
Dornelles, Rita Cássia Menegati [UNESP]
Nakamune, Ana Cláudia de Melo Stevanato [UNESP]
Chaves-Neto, Antonio Hernandes [UNESP]
author_role author
author2 Souza, Rayne Oliveira [UNESP]
Dias, Layani Bertaglia [UNESP]
Ribas, Tayná Buffulin [UNESP]
de Oliveira, Luis Cezar Farias
Sumida, Doris Hissako [UNESP]
Dornelles, Rita Cássia Menegati [UNESP]
Nakamune, Ana Cláudia de Melo Stevanato [UNESP]
Chaves-Neto, Antonio Hernandes [UNESP]
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Paulista University (UNIP)
Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv dos Santos, Damaris Raissa [UNESP]
Souza, Rayne Oliveira [UNESP]
Dias, Layani Bertaglia [UNESP]
Ribas, Tayná Buffulin [UNESP]
de Oliveira, Luis Cezar Farias
Sumida, Doris Hissako [UNESP]
Dornelles, Rita Cássia Menegati [UNESP]
Nakamune, Ana Cláudia de Melo Stevanato [UNESP]
Chaves-Neto, Antonio Hernandes [UNESP]
dc.subject.por.fl_str_mv Dehydrogenase
Phosphatases
Saliva
Storage
Temperature
Transaminases
topic Dehydrogenase
Phosphatases
Saliva
Storage
Temperature
Transaminases
description Objectives To investigate the influence of temperature and storage time on salivary acid phosphatase (ACP), tartrate-resistant acid phosphatase (TRAP), alkaline phosphatase (ALP), aspartate aminotransferase (AST) and alanine aminotransferase (ALT) and lactate dehydrogenase (LDH). Design Unstimulated whole expectorated saliva was collected from healthy men and women subjects (n = 26) between 8 and 10 a.m. The saliva samples were centrifuged, and the supernatants were measured for ACP, TRAP, ALP, AST, ALT and LDH activities immediately (without freezing) [baseline values] and after time intervals of 3, 7, 14 and 28 days (d) of storage at −20 °C and −80 °C using spectrophotometric methods The influence of storage time was analyzed by one-way ANOVA followed by the Dunnett post-test, while the paired Student's-t-test was used to compare the differences between the temperature (p < 0.05). Results There was significant decline in the activities of all enzymes at −20 °C with increasing storage time. This decrease was relevant from day 14 onward for the majority of the enzymes, with the exception of AST. After day 28, the more sensitive enzymes were ALP and LDH, which showed residual activity of 39% and 16%, respectively, compared with baseline values. There were considerable, but insignificant changes, in the activities of all enzymes after storage at −80 °C for 28 days. Conclusions Frozen samples should be kept at −80 °C to preserve these activities, but there are restrictions for the enzymes ALP, ALT and LDH. Storage of samples at −20 °C could introduce high error variance in measured activities.
publishDate 2018
dc.date.none.fl_str_mv 2018-12-11T17:15:34Z
2018-12-11T17:15:34Z
2018-01-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.archoralbio.2017.10.016
Archives of Oral Biology, v. 85, p. 160-165.
1879-1506
0003-9969
http://hdl.handle.net/11449/175378
10.1016/j.archoralbio.2017.10.016
2-s2.0-85032173410
2-s2.0-85032173410.pdf
5435902422784889
0000-0003-0783-6612
url http://dx.doi.org/10.1016/j.archoralbio.2017.10.016
http://hdl.handle.net/11449/175378
identifier_str_mv Archives of Oral Biology, v. 85, p. 160-165.
1879-1506
0003-9969
10.1016/j.archoralbio.2017.10.016
2-s2.0-85032173410
2-s2.0-85032173410.pdf
5435902422784889
0000-0003-0783-6612
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Archives of Oral Biology
0,752
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 160-165
application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv repositoriounesp@unesp.br
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