The effects of storage time and temperature on the stability of salivary phosphatases, transaminases and dehydrogenase
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.archoralbio.2017.10.016 http://hdl.handle.net/11449/175378 |
Resumo: | Objectives To investigate the influence of temperature and storage time on salivary acid phosphatase (ACP), tartrate-resistant acid phosphatase (TRAP), alkaline phosphatase (ALP), aspartate aminotransferase (AST) and alanine aminotransferase (ALT) and lactate dehydrogenase (LDH). Design Unstimulated whole expectorated saliva was collected from healthy men and women subjects (n = 26) between 8 and 10 a.m. The saliva samples were centrifuged, and the supernatants were measured for ACP, TRAP, ALP, AST, ALT and LDH activities immediately (without freezing) [baseline values] and after time intervals of 3, 7, 14 and 28 days (d) of storage at −20 °C and −80 °C using spectrophotometric methods The influence of storage time was analyzed by one-way ANOVA followed by the Dunnett post-test, while the paired Student's-t-test was used to compare the differences between the temperature (p < 0.05). Results There was significant decline in the activities of all enzymes at −20 °C with increasing storage time. This decrease was relevant from day 14 onward for the majority of the enzymes, with the exception of AST. After day 28, the more sensitive enzymes were ALP and LDH, which showed residual activity of 39% and 16%, respectively, compared with baseline values. There were considerable, but insignificant changes, in the activities of all enzymes after storage at −80 °C for 28 days. Conclusions Frozen samples should be kept at −80 °C to preserve these activities, but there are restrictions for the enzymes ALP, ALT and LDH. Storage of samples at −20 °C could introduce high error variance in measured activities. |
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Repositório Institucional da UNESP |
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The effects of storage time and temperature on the stability of salivary phosphatases, transaminases and dehydrogenaseDehydrogenasePhosphatasesSalivaStorageTemperatureTransaminasesObjectives To investigate the influence of temperature and storage time on salivary acid phosphatase (ACP), tartrate-resistant acid phosphatase (TRAP), alkaline phosphatase (ALP), aspartate aminotransferase (AST) and alanine aminotransferase (ALT) and lactate dehydrogenase (LDH). Design Unstimulated whole expectorated saliva was collected from healthy men and women subjects (n = 26) between 8 and 10 a.m. The saliva samples were centrifuged, and the supernatants were measured for ACP, TRAP, ALP, AST, ALT and LDH activities immediately (without freezing) [baseline values] and after time intervals of 3, 7, 14 and 28 days (d) of storage at −20 °C and −80 °C using spectrophotometric methods The influence of storage time was analyzed by one-way ANOVA followed by the Dunnett post-test, while the paired Student's-t-test was used to compare the differences between the temperature (p < 0.05). Results There was significant decline in the activities of all enzymes at −20 °C with increasing storage time. This decrease was relevant from day 14 onward for the majority of the enzymes, with the exception of AST. After day 28, the more sensitive enzymes were ALP and LDH, which showed residual activity of 39% and 16%, respectively, compared with baseline values. There were considerable, but insignificant changes, in the activities of all enzymes after storage at −80 °C for 28 days. Conclusions Frozen samples should be kept at −80 °C to preserve these activities, but there are restrictions for the enzymes ALP, ALT and LDH. Storage of samples at −20 °C could introduce high error variance in measured activities.Paulista University (UNIP), Araçatuba, Avenida Baguaçu, 1939Department of Basic Sciences São Paulo State University (Unesp) School of Dentistry, Araçatuba, Rodovia Marechal Rondon 527/528 km, Campus UniversitárioPrograma Multicêntrico de Pós-Graduação em Ciências Fisiológicas – SBFis São Paulo State University (Unesp) School of Dentistry, Rodovia Marechal Rondon 527/528 km, Campus UniversitárioDepartment of Basic Sciences São Paulo State University (Unesp) School of Dentistry, Araçatuba, Rodovia Marechal Rondon 527/528 km, Campus UniversitárioPrograma Multicêntrico de Pós-Graduação em Ciências Fisiológicas – SBFis São Paulo State University (Unesp) School of Dentistry, Rodovia Marechal Rondon 527/528 km, Campus UniversitárioPaulista University (UNIP)Universidade Estadual Paulista (Unesp)dos Santos, Damaris Raissa [UNESP]Souza, Rayne Oliveira [UNESP]Dias, Layani Bertaglia [UNESP]Ribas, Tayná Buffulin [UNESP]de Oliveira, Luis Cezar FariasSumida, Doris Hissako [UNESP]Dornelles, Rita Cássia Menegati [UNESP]Nakamune, Ana Cláudia de Melo Stevanato [UNESP]Chaves-Neto, Antonio Hernandes [UNESP]2018-12-11T17:15:34Z2018-12-11T17:15:34Z2018-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article160-165application/pdfhttp://dx.doi.org/10.1016/j.archoralbio.2017.10.016Archives of Oral Biology, v. 85, p. 160-165.1879-15060003-9969http://hdl.handle.net/11449/17537810.1016/j.archoralbio.2017.10.0162-s2.0-850321734102-s2.0-85032173410.pdf54359024227848890000-0003-0783-6612Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengArchives of Oral Biology0,752info:eu-repo/semantics/openAccess2024-09-19T14:02:45Zoai:repositorio.unesp.br:11449/175378Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-19T14:02:45Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
The effects of storage time and temperature on the stability of salivary phosphatases, transaminases and dehydrogenase |
title |
The effects of storage time and temperature on the stability of salivary phosphatases, transaminases and dehydrogenase |
spellingShingle |
The effects of storage time and temperature on the stability of salivary phosphatases, transaminases and dehydrogenase dos Santos, Damaris Raissa [UNESP] Dehydrogenase Phosphatases Saliva Storage Temperature Transaminases |
title_short |
The effects of storage time and temperature on the stability of salivary phosphatases, transaminases and dehydrogenase |
title_full |
The effects of storage time and temperature on the stability of salivary phosphatases, transaminases and dehydrogenase |
title_fullStr |
The effects of storage time and temperature on the stability of salivary phosphatases, transaminases and dehydrogenase |
title_full_unstemmed |
The effects of storage time and temperature on the stability of salivary phosphatases, transaminases and dehydrogenase |
title_sort |
The effects of storage time and temperature on the stability of salivary phosphatases, transaminases and dehydrogenase |
author |
dos Santos, Damaris Raissa [UNESP] |
author_facet |
dos Santos, Damaris Raissa [UNESP] Souza, Rayne Oliveira [UNESP] Dias, Layani Bertaglia [UNESP] Ribas, Tayná Buffulin [UNESP] de Oliveira, Luis Cezar Farias Sumida, Doris Hissako [UNESP] Dornelles, Rita Cássia Menegati [UNESP] Nakamune, Ana Cláudia de Melo Stevanato [UNESP] Chaves-Neto, Antonio Hernandes [UNESP] |
author_role |
author |
author2 |
Souza, Rayne Oliveira [UNESP] Dias, Layani Bertaglia [UNESP] Ribas, Tayná Buffulin [UNESP] de Oliveira, Luis Cezar Farias Sumida, Doris Hissako [UNESP] Dornelles, Rita Cássia Menegati [UNESP] Nakamune, Ana Cláudia de Melo Stevanato [UNESP] Chaves-Neto, Antonio Hernandes [UNESP] |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Paulista University (UNIP) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
dos Santos, Damaris Raissa [UNESP] Souza, Rayne Oliveira [UNESP] Dias, Layani Bertaglia [UNESP] Ribas, Tayná Buffulin [UNESP] de Oliveira, Luis Cezar Farias Sumida, Doris Hissako [UNESP] Dornelles, Rita Cássia Menegati [UNESP] Nakamune, Ana Cláudia de Melo Stevanato [UNESP] Chaves-Neto, Antonio Hernandes [UNESP] |
dc.subject.por.fl_str_mv |
Dehydrogenase Phosphatases Saliva Storage Temperature Transaminases |
topic |
Dehydrogenase Phosphatases Saliva Storage Temperature Transaminases |
description |
Objectives To investigate the influence of temperature and storage time on salivary acid phosphatase (ACP), tartrate-resistant acid phosphatase (TRAP), alkaline phosphatase (ALP), aspartate aminotransferase (AST) and alanine aminotransferase (ALT) and lactate dehydrogenase (LDH). Design Unstimulated whole expectorated saliva was collected from healthy men and women subjects (n = 26) between 8 and 10 a.m. The saliva samples were centrifuged, and the supernatants were measured for ACP, TRAP, ALP, AST, ALT and LDH activities immediately (without freezing) [baseline values] and after time intervals of 3, 7, 14 and 28 days (d) of storage at −20 °C and −80 °C using spectrophotometric methods The influence of storage time was analyzed by one-way ANOVA followed by the Dunnett post-test, while the paired Student's-t-test was used to compare the differences between the temperature (p < 0.05). Results There was significant decline in the activities of all enzymes at −20 °C with increasing storage time. This decrease was relevant from day 14 onward for the majority of the enzymes, with the exception of AST. After day 28, the more sensitive enzymes were ALP and LDH, which showed residual activity of 39% and 16%, respectively, compared with baseline values. There were considerable, but insignificant changes, in the activities of all enzymes after storage at −80 °C for 28 days. Conclusions Frozen samples should be kept at −80 °C to preserve these activities, but there are restrictions for the enzymes ALP, ALT and LDH. Storage of samples at −20 °C could introduce high error variance in measured activities. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-12-11T17:15:34Z 2018-12-11T17:15:34Z 2018-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.archoralbio.2017.10.016 Archives of Oral Biology, v. 85, p. 160-165. 1879-1506 0003-9969 http://hdl.handle.net/11449/175378 10.1016/j.archoralbio.2017.10.016 2-s2.0-85032173410 2-s2.0-85032173410.pdf 5435902422784889 0000-0003-0783-6612 |
url |
http://dx.doi.org/10.1016/j.archoralbio.2017.10.016 http://hdl.handle.net/11449/175378 |
identifier_str_mv |
Archives of Oral Biology, v. 85, p. 160-165. 1879-1506 0003-9969 10.1016/j.archoralbio.2017.10.016 2-s2.0-85032173410 2-s2.0-85032173410.pdf 5435902422784889 0000-0003-0783-6612 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Archives of Oral Biology 0,752 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
160-165 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
repositoriounesp@unesp.br |
_version_ |
1813546426630668288 |