Persistence of Staphylococcus spp. in milk from cows undergoing homeopathy to control subclinical mastitis
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1186/s12917-022-03364-8 http://hdl.handle.net/11449/241325 |
Resumo: | Background: Mastitis is one of the major diseases in dairy cattle, as it causes great economic losses to producers due to the reduction of milk production and changes in the quality of the product. The disease is mainly caused by bacteria of the genus Staphylococcus spp., these microorganisms can express various virulence factors, such as biofilms for example. In herds with organic management, producers and technicians use unconventional ways to treat and control the disease, such as homeopathy. However, it is not known if this type of treatment is able to control pathogenic bacteria such as those of the genus Staphylococcus, of relevance to animal and human health. Thus, the objective of this study was to investigate the production of biofilm in vitro and its genes by Staphylococcus spp. isolated in the milk of cows treated with homeopathy, as well as the persistence of microorganisms in animals. Methods: Ninety-nine isolates of Staphylococcus spp. from cows treated and not treated with homeopathy were identified by internal transcribed space-polymerase chain reaction and investigated for the presence of the icaABCD, bap, aap, atlE, and bhp genes and in vitro biofilm production using the adhesion method on polystyrene plates. The enzyme restriction profile was determined by Pulsed-Field Gel Electrophoresis. Clusters of S. aureus and S. epidermidis with three or more isolates had an isolate selected for Multilocus Sequence Typing. Results: The frequency of S. aureus isolations was similar in treated and untreated cows, while 71.4% of the coagulase-negative identified were isolated in cows treated with homeopathy. The distribution of the operon ica genes was similar in animals with and without treatment, except for the icaD gene, more frequent in treated cows. Production of biofilm was associated with presence of one or more genes from the icaADBC operon. S. aureus revealed a greater diversity and greater dissemination in cows treated and not treated with homeopathy. Sequence Types ST1, ST5, and ST126 were identified in S. aureus. Conclusions: The presence of biofilm-associated genes and the in vitro production of biofilms, combined with the persistence of clonal profiles of Staphylococcus spp. demonstrate other forms of control for bovine mastitis should be researched for organic production herds. |
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Persistence of Staphylococcus spp. in milk from cows undergoing homeopathy to control subclinical mastitisBiofilmClonal profileicaADBCBackground: Mastitis is one of the major diseases in dairy cattle, as it causes great economic losses to producers due to the reduction of milk production and changes in the quality of the product. The disease is mainly caused by bacteria of the genus Staphylococcus spp., these microorganisms can express various virulence factors, such as biofilms for example. In herds with organic management, producers and technicians use unconventional ways to treat and control the disease, such as homeopathy. However, it is not known if this type of treatment is able to control pathogenic bacteria such as those of the genus Staphylococcus, of relevance to animal and human health. Thus, the objective of this study was to investigate the production of biofilm in vitro and its genes by Staphylococcus spp. isolated in the milk of cows treated with homeopathy, as well as the persistence of microorganisms in animals. Methods: Ninety-nine isolates of Staphylococcus spp. from cows treated and not treated with homeopathy were identified by internal transcribed space-polymerase chain reaction and investigated for the presence of the icaABCD, bap, aap, atlE, and bhp genes and in vitro biofilm production using the adhesion method on polystyrene plates. The enzyme restriction profile was determined by Pulsed-Field Gel Electrophoresis. Clusters of S. aureus and S. epidermidis with three or more isolates had an isolate selected for Multilocus Sequence Typing. Results: The frequency of S. aureus isolations was similar in treated and untreated cows, while 71.4% of the coagulase-negative identified were isolated in cows treated with homeopathy. The distribution of the operon ica genes was similar in animals with and without treatment, except for the icaD gene, more frequent in treated cows. Production of biofilm was associated with presence of one or more genes from the icaADBC operon. S. aureus revealed a greater diversity and greater dissemination in cows treated and not treated with homeopathy. Sequence Types ST1, ST5, and ST126 were identified in S. aureus. Conclusions: The presence of biofilm-associated genes and the in vitro production of biofilms, combined with the persistence of clonal profiles of Staphylococcus spp. demonstrate other forms of control for bovine mastitis should be researched for organic production herds.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Department of Pathology Reproduction and One Health Paulista State University “Júlio de Mesquita Filho” - FCAV, JaboticabalDepartment of Microbiology and Immunology Paulista State University “Júlio de Mesquita Filho” - IB, BotucatuEmbrapa Southeast Livestock Canchim FarmBacteriology Center Adolfo Lutz InstituteDepartment of Pathology Reproduction and One Health Paulista State University “Júlio de Mesquita Filho” - FCAV, JaboticabalDepartment of Microbiology and Immunology Paulista State University “Júlio de Mesquita Filho” - IB, BotucatuCAPES: 001FAPESP: 2017/08979/7Universidade Estadual Paulista (UNESP)Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)Adolfo Lutz InstituteFerreira, Elka Machado [UNESP]Romero, Letícia Castilho [UNESP]Cunha, Maria de Lourdes Ribeiro de Souza da [UNESP]Malagó Junior, WilsonCamargo, Carlos HenriqueBarioni Júnior, WaldomiroZafalon, Luiz Francisco2023-03-01T20:56:59Z2023-03-01T20:56:59Z2022-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1186/s12917-022-03364-8BMC Veterinary Research, v. 18, n. 1, 2022.1746-6148http://hdl.handle.net/11449/24132510.1186/s12917-022-03364-82-s2.0-85133998317Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBMC Veterinary Researchinfo:eu-repo/semantics/openAccess2024-06-07T13:03:07Zoai:repositorio.unesp.br:11449/241325Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T22:40:08.670953Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Persistence of Staphylococcus spp. in milk from cows undergoing homeopathy to control subclinical mastitis |
title |
Persistence of Staphylococcus spp. in milk from cows undergoing homeopathy to control subclinical mastitis |
spellingShingle |
Persistence of Staphylococcus spp. in milk from cows undergoing homeopathy to control subclinical mastitis Ferreira, Elka Machado [UNESP] Biofilm Clonal profile icaADBC |
title_short |
Persistence of Staphylococcus spp. in milk from cows undergoing homeopathy to control subclinical mastitis |
title_full |
Persistence of Staphylococcus spp. in milk from cows undergoing homeopathy to control subclinical mastitis |
title_fullStr |
Persistence of Staphylococcus spp. in milk from cows undergoing homeopathy to control subclinical mastitis |
title_full_unstemmed |
Persistence of Staphylococcus spp. in milk from cows undergoing homeopathy to control subclinical mastitis |
title_sort |
Persistence of Staphylococcus spp. in milk from cows undergoing homeopathy to control subclinical mastitis |
author |
Ferreira, Elka Machado [UNESP] |
author_facet |
Ferreira, Elka Machado [UNESP] Romero, Letícia Castilho [UNESP] Cunha, Maria de Lourdes Ribeiro de Souza da [UNESP] Malagó Junior, Wilson Camargo, Carlos Henrique Barioni Júnior, Waldomiro Zafalon, Luiz Francisco |
author_role |
author |
author2 |
Romero, Letícia Castilho [UNESP] Cunha, Maria de Lourdes Ribeiro de Souza da [UNESP] Malagó Junior, Wilson Camargo, Carlos Henrique Barioni Júnior, Waldomiro Zafalon, Luiz Francisco |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA) Adolfo Lutz Institute |
dc.contributor.author.fl_str_mv |
Ferreira, Elka Machado [UNESP] Romero, Letícia Castilho [UNESP] Cunha, Maria de Lourdes Ribeiro de Souza da [UNESP] Malagó Junior, Wilson Camargo, Carlos Henrique Barioni Júnior, Waldomiro Zafalon, Luiz Francisco |
dc.subject.por.fl_str_mv |
Biofilm Clonal profile icaADBC |
topic |
Biofilm Clonal profile icaADBC |
description |
Background: Mastitis is one of the major diseases in dairy cattle, as it causes great economic losses to producers due to the reduction of milk production and changes in the quality of the product. The disease is mainly caused by bacteria of the genus Staphylococcus spp., these microorganisms can express various virulence factors, such as biofilms for example. In herds with organic management, producers and technicians use unconventional ways to treat and control the disease, such as homeopathy. However, it is not known if this type of treatment is able to control pathogenic bacteria such as those of the genus Staphylococcus, of relevance to animal and human health. Thus, the objective of this study was to investigate the production of biofilm in vitro and its genes by Staphylococcus spp. isolated in the milk of cows treated with homeopathy, as well as the persistence of microorganisms in animals. Methods: Ninety-nine isolates of Staphylococcus spp. from cows treated and not treated with homeopathy were identified by internal transcribed space-polymerase chain reaction and investigated for the presence of the icaABCD, bap, aap, atlE, and bhp genes and in vitro biofilm production using the adhesion method on polystyrene plates. The enzyme restriction profile was determined by Pulsed-Field Gel Electrophoresis. Clusters of S. aureus and S. epidermidis with three or more isolates had an isolate selected for Multilocus Sequence Typing. Results: The frequency of S. aureus isolations was similar in treated and untreated cows, while 71.4% of the coagulase-negative identified were isolated in cows treated with homeopathy. The distribution of the operon ica genes was similar in animals with and without treatment, except for the icaD gene, more frequent in treated cows. Production of biofilm was associated with presence of one or more genes from the icaADBC operon. S. aureus revealed a greater diversity and greater dissemination in cows treated and not treated with homeopathy. Sequence Types ST1, ST5, and ST126 were identified in S. aureus. Conclusions: The presence of biofilm-associated genes and the in vitro production of biofilms, combined with the persistence of clonal profiles of Staphylococcus spp. demonstrate other forms of control for bovine mastitis should be researched for organic production herds. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-12-01 2023-03-01T20:56:59Z 2023-03-01T20:56:59Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1186/s12917-022-03364-8 BMC Veterinary Research, v. 18, n. 1, 2022. 1746-6148 http://hdl.handle.net/11449/241325 10.1186/s12917-022-03364-8 2-s2.0-85133998317 |
url |
http://dx.doi.org/10.1186/s12917-022-03364-8 http://hdl.handle.net/11449/241325 |
identifier_str_mv |
BMC Veterinary Research, v. 18, n. 1, 2022. 1746-6148 10.1186/s12917-022-03364-8 2-s2.0-85133998317 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
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BMC Veterinary Research |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
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UNESP |
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UNESP |
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Repositório Institucional da UNESP |
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Repositório Institucional da UNESP |
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Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
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1808129448672755712 |