A highly specific and sensitive nanoimmunosensor for the diagnosis of neuromyelitis optica spectrum disorders

Detalhes bibliográficos
Autor(a) principal: Moraes, Ariana de Souza
Data de Publicação: 2019
Outros Autores: Brum, Doralina Guimaraes [UNESP], Magalhaes Ierich, Jessica Cristiane, Higa, Akemi Martins, Jabur Assis, Amanda Stefanie, Miyazaki, Celina Massumi, Shimizu, Flavio Makoto, Peroni, Luis Antonio, Machini, M. Teresa, Barreira, Amilton Antunes, Ferreira, Marystela, Oliveira Jr, Osvaldo N., Leite, Fabio Lima
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1038/s41598-019-52506-w
http://hdl.handle.net/11449/196300
Resumo: A precise diagnosis for neuromyelitis optica spectrum disorders (NMOSD) is crucial to improve patients' prognostic, which requires highly specific and sensitive tests. The cell-based assay with a sensitivity of 76% and specificity of 100% is the most recommended test to detect anti-aquaporin-4 antibodies (AQP4-Ab). Here, we tested four AQP4 external loop peptides (AQP4(61-70), AQP4(131-140), AQP4(141-150), and AQP4(201-210)) with an atomic force microscopy nanoimmunosensor to develop a diagnostic assay. We obtained the highest reactivity with AQP4(61-70)-nanoimunosensor. This assay was effective in detecting AQP4-Ab in sera of NMOSD patients with 100% specificity (95% CI 63.06-100), determined by the cut-off adhesion force value of 241.3 pN. NMOSD patients were successfully discriminated from a set of healthy volunteers, patients with multiple sclerosis, and AQP4-Ab-negative patients. AQP4(61-70) sensitivity was 81.25% (95% CI 56.50-99.43), slightly higher than with the CBA method. The results with the AQP4(61-70)-nanoimmunosensor indicate that the differences between NMOSD seropositive and seronegative phenotypes are related to disease-specific epitopes. The absence of AQP4-Ab in sera of NMOSD AQP4-Ab-negative patients may be interpreted by assuming the existence of another potential AQP4 peptide sequence or non-AQP4 antigens as the antibody target.
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spelling A highly specific and sensitive nanoimmunosensor for the diagnosis of neuromyelitis optica spectrum disordersA precise diagnosis for neuromyelitis optica spectrum disorders (NMOSD) is crucial to improve patients' prognostic, which requires highly specific and sensitive tests. The cell-based assay with a sensitivity of 76% and specificity of 100% is the most recommended test to detect anti-aquaporin-4 antibodies (AQP4-Ab). Here, we tested four AQP4 external loop peptides (AQP4(61-70), AQP4(131-140), AQP4(141-150), and AQP4(201-210)) with an atomic force microscopy nanoimmunosensor to develop a diagnostic assay. We obtained the highest reactivity with AQP4(61-70)-nanoimunosensor. This assay was effective in detecting AQP4-Ab in sera of NMOSD patients with 100% specificity (95% CI 63.06-100), determined by the cut-off adhesion force value of 241.3 pN. NMOSD patients were successfully discriminated from a set of healthy volunteers, patients with multiple sclerosis, and AQP4-Ab-negative patients. AQP4(61-70) sensitivity was 81.25% (95% CI 56.50-99.43), slightly higher than with the CBA method. The results with the AQP4(61-70)-nanoimmunosensor indicate that the differences between NMOSD seropositive and seronegative phenotypes are related to disease-specific epitopes. The absence of AQP4-Ab in sera of NMOSD AQP4-Ab-negative patients may be interpreted by assuming the existence of another potential AQP4 peptide sequence or non-AQP4 antigens as the antibody target.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Brazil National Council for Scientific and Technological DevelopmentNational Institute for Science and Technology on Organic Electronics -INEOUniv Sao Paulo, Inst Trop Med Sao Paulo, BR-05403000 Sao Paulo, SP, BrazilUniv Fed Sao Carlos, Dept Phys Chem & Math, BR-18052780 Sorocaba, SP, BrazilUniv Fed Sao Carlos, Nanoneurobiophys Res Grp GNN, BR-18052780 Sorocaba, SP, BrazilSao Paulo State Univ, Dept Neurol Psychol & Psychiat, BR-18618687 Botucatu, SP, BrazilUniv Sao Paulo, Sao Carlos Inst Phys, BR-13560970 Sao Carlos, SP, BrazilRheabiotech Lab Res & Dev, Campinas, SP, BrazilUniv Sao Paulo, Inst Chem, Dept Biochem, BR-05508000 Sao Paulo, BrazilUniv Sao Paulo, Ribeirao Preto Med Sch, Dept Neurosci & Behav Sci, Ribeirao Preto, SP, BrazilSao Paulo State Univ, Dept Neurol Psychol & Psychiat, BR-18618687 Botucatu, SP, BrazilFAPESP: FAPESP 2013/14262-7FAPESP: 2015/05283-6FAPESP: 2015/06847-0FAPESP: 2014/12082-4FAPESP: 2014/15093-7FAPESP: 2016/19387-0FAPESP: 2015/36143-2FAPESP: 2015/14360-4FAPESP: 2012/50839-4CAPES: 001Brazil National Council for Scientific and Technological Development: CNPq 305069/2016-0Brazil National Council for Scientific and Technological Development: 459768/2014-0Brazil National Council for Scientific and Technological Development: 308570/2018-9Brazil National Council for Scientific and Technological Development: 308658/2015-9National Institute for Science and Technology on Organic Electronics -INEO: CNPq 465572/2014-6National Institute for Science and Technology on Organic Electronics -INEO: FAPESP 2014/50869-6National Institute for Science and Technology on Organic Electronics -INEO: CAPES 23038.000776/201754Nature Publishing GroupUniversidade de São Paulo (USP)Universidade Federal de São Carlos (UFSCar)Universidade Estadual Paulista (Unesp)Rheabiotech Lab Res & DevMoraes, Ariana de SouzaBrum, Doralina Guimaraes [UNESP]Magalhaes Ierich, Jessica CristianeHiga, Akemi MartinsJabur Assis, Amanda StefanieMiyazaki, Celina MassumiShimizu, Flavio MakotoPeroni, Luis AntonioMachini, M. TeresaBarreira, Amilton AntunesFerreira, MarystelaOliveira Jr, Osvaldo N.Leite, Fabio Lima2020-12-10T19:40:10Z2020-12-10T19:40:10Z2019-11-06info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article9http://dx.doi.org/10.1038/s41598-019-52506-wScientific Reports. London: Nature Publishing Group, v. 9, 9 p., 2019.2045-2322http://hdl.handle.net/11449/19630010.1038/s41598-019-52506-wWOS:000494636500025Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengScientific Reportsinfo:eu-repo/semantics/openAccess2024-08-16T15:46:17Zoai:repositorio.unesp.br:11449/196300Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-16T15:46:17Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv A highly specific and sensitive nanoimmunosensor for the diagnosis of neuromyelitis optica spectrum disorders
title A highly specific and sensitive nanoimmunosensor for the diagnosis of neuromyelitis optica spectrum disorders
spellingShingle A highly specific and sensitive nanoimmunosensor for the diagnosis of neuromyelitis optica spectrum disorders
Moraes, Ariana de Souza
title_short A highly specific and sensitive nanoimmunosensor for the diagnosis of neuromyelitis optica spectrum disorders
title_full A highly specific and sensitive nanoimmunosensor for the diagnosis of neuromyelitis optica spectrum disorders
title_fullStr A highly specific and sensitive nanoimmunosensor for the diagnosis of neuromyelitis optica spectrum disorders
title_full_unstemmed A highly specific and sensitive nanoimmunosensor for the diagnosis of neuromyelitis optica spectrum disorders
title_sort A highly specific and sensitive nanoimmunosensor for the diagnosis of neuromyelitis optica spectrum disorders
author Moraes, Ariana de Souza
author_facet Moraes, Ariana de Souza
Brum, Doralina Guimaraes [UNESP]
Magalhaes Ierich, Jessica Cristiane
Higa, Akemi Martins
Jabur Assis, Amanda Stefanie
Miyazaki, Celina Massumi
Shimizu, Flavio Makoto
Peroni, Luis Antonio
Machini, M. Teresa
Barreira, Amilton Antunes
Ferreira, Marystela
Oliveira Jr, Osvaldo N.
Leite, Fabio Lima
author_role author
author2 Brum, Doralina Guimaraes [UNESP]
Magalhaes Ierich, Jessica Cristiane
Higa, Akemi Martins
Jabur Assis, Amanda Stefanie
Miyazaki, Celina Massumi
Shimizu, Flavio Makoto
Peroni, Luis Antonio
Machini, M. Teresa
Barreira, Amilton Antunes
Ferreira, Marystela
Oliveira Jr, Osvaldo N.
Leite, Fabio Lima
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade de São Paulo (USP)
Universidade Federal de São Carlos (UFSCar)
Universidade Estadual Paulista (Unesp)
Rheabiotech Lab Res & Dev
dc.contributor.author.fl_str_mv Moraes, Ariana de Souza
Brum, Doralina Guimaraes [UNESP]
Magalhaes Ierich, Jessica Cristiane
Higa, Akemi Martins
Jabur Assis, Amanda Stefanie
Miyazaki, Celina Massumi
Shimizu, Flavio Makoto
Peroni, Luis Antonio
Machini, M. Teresa
Barreira, Amilton Antunes
Ferreira, Marystela
Oliveira Jr, Osvaldo N.
Leite, Fabio Lima
description A precise diagnosis for neuromyelitis optica spectrum disorders (NMOSD) is crucial to improve patients' prognostic, which requires highly specific and sensitive tests. The cell-based assay with a sensitivity of 76% and specificity of 100% is the most recommended test to detect anti-aquaporin-4 antibodies (AQP4-Ab). Here, we tested four AQP4 external loop peptides (AQP4(61-70), AQP4(131-140), AQP4(141-150), and AQP4(201-210)) with an atomic force microscopy nanoimmunosensor to develop a diagnostic assay. We obtained the highest reactivity with AQP4(61-70)-nanoimunosensor. This assay was effective in detecting AQP4-Ab in sera of NMOSD patients with 100% specificity (95% CI 63.06-100), determined by the cut-off adhesion force value of 241.3 pN. NMOSD patients were successfully discriminated from a set of healthy volunteers, patients with multiple sclerosis, and AQP4-Ab-negative patients. AQP4(61-70) sensitivity was 81.25% (95% CI 56.50-99.43), slightly higher than with the CBA method. The results with the AQP4(61-70)-nanoimmunosensor indicate that the differences between NMOSD seropositive and seronegative phenotypes are related to disease-specific epitopes. The absence of AQP4-Ab in sera of NMOSD AQP4-Ab-negative patients may be interpreted by assuming the existence of another potential AQP4 peptide sequence or non-AQP4 antigens as the antibody target.
publishDate 2019
dc.date.none.fl_str_mv 2019-11-06
2020-12-10T19:40:10Z
2020-12-10T19:40:10Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1038/s41598-019-52506-w
Scientific Reports. London: Nature Publishing Group, v. 9, 9 p., 2019.
2045-2322
http://hdl.handle.net/11449/196300
10.1038/s41598-019-52506-w
WOS:000494636500025
url http://dx.doi.org/10.1038/s41598-019-52506-w
http://hdl.handle.net/11449/196300
identifier_str_mv Scientific Reports. London: Nature Publishing Group, v. 9, 9 p., 2019.
2045-2322
10.1038/s41598-019-52506-w
WOS:000494636500025
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Scientific Reports
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.format.none.fl_str_mv 9
dc.publisher.none.fl_str_mv Nature Publishing Group
publisher.none.fl_str_mv Nature Publishing Group
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
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institution UNESP
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collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
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