Separação imunomagnética associada a bacteriófago para diagnóstico de Salmonella enterica em carne de frango

• Autor(a) principal: Corrêa, Isadora Mainieri de Oliveira [UNESP]
• Data de Publicação: 2015
• Tipo de documento: Tese
• Idioma: por
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://hdl.handle.net/11449/138467; http://www.athena.biblioteca.unesp.br/exlibris/bd/cathedra/09-05-2016/000858305.pdf
• topic: Salmonella enteritidis; Salmonella typhimurium; Frango de corte; Fago; Diagnostico bacteriologico

We used the Immunomagnectic Separarion Assay associated with Bacteriophage to detect the following serovars: Salmonella Enteritidis, Salmonella Heidelberg and Salmonella Typhimurium, in poultry drumstick samples artificially contaminated. We compared the efficiency of this technique to the usual diagnostic tests for Salmonella in food samples: the standard bacteriological analysis, which includes the step of pre-enrichment and selective enrichment, biochemical and serological screening tests, and polymerase chain reaction (PCR). To evaluate the tests capability of Salmonella detection poultry meat samples were submitted to artificial contamination with 5, 10 and 100 CFU/25mL of bacteria, for each of the serovars listed above, totaling 270 analyzes divided into 90 tests for each of the three serovars, and so compare the results obtained with the bacteriological analysis, PCR and Immunomagnetic Separation Bacteriophage assay. We found that the Immunomagnetic Separation Bacteriophage assay was comparable to bacteriological method recommended by the Ministry of Agriculture, Livestock and Supply (MAPA) and the PCR technique, because 99.6% of the samples were positive to accomplish Immunomagnetic Separation Bacteriophage assay and only a sample of Salmonella Enteritidis was negative in this test, in the concentration of 5 CFU/25mL. The bacteriological method check 95.5% positivity, with nine samples negative for S. Heidelberg, two negative for S. Enteritidis and two in the test with S. Typhimurium. In PCR 98.5% positives samples were obtained, with one S. Enteritidis negative sample in the concentration of 5 CFU/25ml and three negative samples for S. Typhimurium. The time for performing each test was measured and the Immunomagnetic Separation Bacteriophage assay was the most rapid test for Salmonella diagnosis, since 20 hours, the conventional bacteriological took 88h and PCR 44h approximately. In this study we confirm the effectiveness of the ...