Essential oils as natural fungicides to control rhizopus stolonifer-induced spoiled of strawberries

Detalhes bibliográficos
Autor(a) principal: Filho, Josemar Gonçalves Oliveira [UNESP]
Data de Publicação: 2021
Outros Autores: Silva, Guilherme da Cruz, Egea, Mariana Buranelo, de Azeredo, Henriette Monteiro Cordeiro, Ferreira, Marcos David
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.33263/BRIAC115.1324413251
http://hdl.handle.net/11449/205902
Resumo: Strawberry is a highly desirable fruit with a unique taste and a good source of bioactive compounds beneficial to human health. However, it has a short post-harvest shelf life, mainly due to the soft rot caused by Rhizopus stolonifer. This study aimed to evaluate the antimicrobial properties of essential oils (EOs) of Mentha piperita, Cymbopogon martinii, Cinnamomum camphora, and Mentha spicata using spore germination and micro-well dilution assays, and to test the effects of the vaporphase application of M. spicata and C. martini on the incidence and severity of soft rot in strawberry artificially inoculated with R. stolonifer. In in vitro tests, C. martinii and M. spicata EOs were the most effective, inhibiting more than 95% of the spore germination. Additionally, in the microwell dilution test, these EOs had the lowest minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) (5 and 10 μg/mL, respectively), and for the microwell dilution test, the lowest MIC (5 μg/mL and 10 μg/mL, respectively) and MFC (10 μg/mL for both). High in vivo inhibitory effects of M. spicata and C. martinii EOs were observed at 10% concentration, with 100 and 78% reduction, respectively, in the R. stolonifer-induced spoilage. Our results suggest that C. martinii and M. spicata EOs can be used as efficient natural fungicides and can be an alternative to synthetic fungicides for preserving fresh strawberries from soft rot.
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spelling Essential oils as natural fungicides to control rhizopus stolonifer-induced spoiled of strawberriesMinimal inhibitory concentrationNatural antifungal agentPlant essential oilsSoft rotStrawberry is a highly desirable fruit with a unique taste and a good source of bioactive compounds beneficial to human health. However, it has a short post-harvest shelf life, mainly due to the soft rot caused by Rhizopus stolonifer. This study aimed to evaluate the antimicrobial properties of essential oils (EOs) of Mentha piperita, Cymbopogon martinii, Cinnamomum camphora, and Mentha spicata using spore germination and micro-well dilution assays, and to test the effects of the vaporphase application of M. spicata and C. martini on the incidence and severity of soft rot in strawberry artificially inoculated with R. stolonifer. In in vitro tests, C. martinii and M. spicata EOs were the most effective, inhibiting more than 95% of the spore germination. Additionally, in the microwell dilution test, these EOs had the lowest minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) (5 and 10 μg/mL, respectively), and for the microwell dilution test, the lowest MIC (5 μg/mL and 10 μg/mL, respectively) and MFC (10 μg/mL for both). High in vivo inhibitory effects of M. spicata and C. martinii EOs were observed at 10% concentration, with 100 and 78% reduction, respectively, in the R. stolonifer-induced spoilage. Our results suggest that C. martinii and M. spicata EOs can be used as efficient natural fungicides and can be an alternative to synthetic fungicides for preserving fresh strawberries from soft rot.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)São Paulo State University (UNESP) School of Pharmaceutical SciencesDepartment of Biotechnology Federal University of São CarlosGoiano Federal Institute of Education Science and TechnologyBrazilian Agricultural Research Corporation Embrapa Agroindústria TropicalBrazilian Agricultural Research Corporation Embrapa InstrumentaçãoSão Paulo State University (UNESP) School of Pharmaceutical SciencesCNPq: (302381/2016-3CAPES: 001CNPq: 101117/2018-3FAPESP: 2018/24612-9CNPq: 407956/2016-6Universidade Estadual Paulista (Unesp)Universidade Federal de São Carlos (UFSCar)and TechnologyEmpresa Brasileira de Pesquisa Agropecuária (EMBRAPA)Filho, Josemar Gonçalves Oliveira [UNESP]Silva, Guilherme da CruzEgea, Mariana Buranelode Azeredo, Henriette Monteiro CordeiroFerreira, Marcos David2021-06-25T10:23:09Z2021-06-25T10:23:09Z2021-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article13244-13251http://dx.doi.org/10.33263/BRIAC115.1324413251Biointerface Research in Applied Chemistry, v. 11, n. 5, p. 13244-13251, 2021.2069-5837http://hdl.handle.net/11449/20590210.33263/BRIAC115.13244132512-s2.0-85101082010Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBiointerface Research in Applied Chemistryinfo:eu-repo/semantics/openAccess2021-10-22T12:25:13Zoai:repositorio.unesp.br:11449/205902Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-22T12:25:13Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Essential oils as natural fungicides to control rhizopus stolonifer-induced spoiled of strawberries
title Essential oils as natural fungicides to control rhizopus stolonifer-induced spoiled of strawberries
spellingShingle Essential oils as natural fungicides to control rhizopus stolonifer-induced spoiled of strawberries
Filho, Josemar Gonçalves Oliveira [UNESP]
Minimal inhibitory concentration
Natural antifungal agent
Plant essential oils
Soft rot
title_short Essential oils as natural fungicides to control rhizopus stolonifer-induced spoiled of strawberries
title_full Essential oils as natural fungicides to control rhizopus stolonifer-induced spoiled of strawberries
title_fullStr Essential oils as natural fungicides to control rhizopus stolonifer-induced spoiled of strawberries
title_full_unstemmed Essential oils as natural fungicides to control rhizopus stolonifer-induced spoiled of strawberries
title_sort Essential oils as natural fungicides to control rhizopus stolonifer-induced spoiled of strawberries
author Filho, Josemar Gonçalves Oliveira [UNESP]
author_facet Filho, Josemar Gonçalves Oliveira [UNESP]
Silva, Guilherme da Cruz
Egea, Mariana Buranelo
de Azeredo, Henriette Monteiro Cordeiro
Ferreira, Marcos David
author_role author
author2 Silva, Guilherme da Cruz
Egea, Mariana Buranelo
de Azeredo, Henriette Monteiro Cordeiro
Ferreira, Marcos David
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Universidade Federal de São Carlos (UFSCar)
and Technology
Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)
dc.contributor.author.fl_str_mv Filho, Josemar Gonçalves Oliveira [UNESP]
Silva, Guilherme da Cruz
Egea, Mariana Buranelo
de Azeredo, Henriette Monteiro Cordeiro
Ferreira, Marcos David
dc.subject.por.fl_str_mv Minimal inhibitory concentration
Natural antifungal agent
Plant essential oils
Soft rot
topic Minimal inhibitory concentration
Natural antifungal agent
Plant essential oils
Soft rot
description Strawberry is a highly desirable fruit with a unique taste and a good source of bioactive compounds beneficial to human health. However, it has a short post-harvest shelf life, mainly due to the soft rot caused by Rhizopus stolonifer. This study aimed to evaluate the antimicrobial properties of essential oils (EOs) of Mentha piperita, Cymbopogon martinii, Cinnamomum camphora, and Mentha spicata using spore germination and micro-well dilution assays, and to test the effects of the vaporphase application of M. spicata and C. martini on the incidence and severity of soft rot in strawberry artificially inoculated with R. stolonifer. In in vitro tests, C. martinii and M. spicata EOs were the most effective, inhibiting more than 95% of the spore germination. Additionally, in the microwell dilution test, these EOs had the lowest minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) (5 and 10 μg/mL, respectively), and for the microwell dilution test, the lowest MIC (5 μg/mL and 10 μg/mL, respectively) and MFC (10 μg/mL for both). High in vivo inhibitory effects of M. spicata and C. martinii EOs were observed at 10% concentration, with 100 and 78% reduction, respectively, in the R. stolonifer-induced spoilage. Our results suggest that C. martinii and M. spicata EOs can be used as efficient natural fungicides and can be an alternative to synthetic fungicides for preserving fresh strawberries from soft rot.
publishDate 2021
dc.date.none.fl_str_mv 2021-06-25T10:23:09Z
2021-06-25T10:23:09Z
2021-01-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.33263/BRIAC115.1324413251
Biointerface Research in Applied Chemistry, v. 11, n. 5, p. 13244-13251, 2021.
2069-5837
http://hdl.handle.net/11449/205902
10.33263/BRIAC115.1324413251
2-s2.0-85101082010
url http://dx.doi.org/10.33263/BRIAC115.1324413251
http://hdl.handle.net/11449/205902
identifier_str_mv Biointerface Research in Applied Chemistry, v. 11, n. 5, p. 13244-13251, 2021.
2069-5837
10.33263/BRIAC115.1324413251
2-s2.0-85101082010
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Biointerface Research in Applied Chemistry
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 13244-13251
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1803047344450568192

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