Diacetylcurcumin: a new photosensitizer for antimicrobial photodynamic therapy in Streptococcus mutans biofilms

• Autor(a) principal: Garbuggio Sanches, Carolina Veronez
• Data de Publicação: 2019
• Outros Autores: Orlandi Sardi, Janaina de Cassia, Suga Terada, Raquel Sano, Lazarini, Josy Goldoni, Freires, Irlan Almeida, Polaquini, Carlos Roberto [UNESP], Torrezan, Guilherme Silva [UNESP], Regasini, Luis Octavio [UNESP], Fujimaki, Mitsue, Rosalen, Pedro Luiz
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://dx.doi.org/10.1080/08927014.2019.1606907; http://hdl.handle.net/11449/185757
• topic: Dental caries; photodynamic therapy; biofilms; Streptococcus mutans; Galleria mellonella; diacetylcurcumin

This study evaluated the effect of antimicrobial photodynamic therapy (aPDT) on S. mutans using diacetylcurcumin (DAC) and verified DAC toxicity. In vitro, S. mutans biofilms were exposed to curcumin (CUR) and DAC and were light-irradiated. Biofilms were collected, plated and incubated for colony counts. DAC and CUR toxicity assays were conducted with Human Gingival Fibroblast cells (HGF). In vivo, G. mellonella larvae were injected with S. mutans and treated with DAC, CUR and aPDT. The hemolymph was plated and incubated for colony counts. Significant reductions were observed when DAC and CUR alone were used and when aPDT was applied. HGF assays demonstrated no differences in cell viability for most groups. DAC and CUR reduced the S. mutans load in G. mellonella larvae both alone and with aPDT. Systematic toxicity assays on G. mellonella demonstrated no effect of DAC and CUR or aPDT on the survival curve.