Amino acid, Antioxidant and Ion Profiles of Carpolobia lutea Leaf (Polygalaceae)

Detalhes bibliográficos
Autor(a) principal: Nwidu, Lucky Legbosi
Data de Publicação: 2012
Outros Autores: Cilli, Eduardo Maffud [UNESP], Vilegas, Wagner [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.4314/tjpr.v11i5.15
http://hdl.handle.net/11449/25259
Resumo: Purpose: To evaluate the amino acid, antioxidant and ionic profiles of Carpolobia lutea leaf (Polygalaceae) extract (CLL).Methods: The powdered leaf was macerated and subjected to gradient solvent extraction with n-hexane, chloroform, ethyl acetate and ethanol for 72 h to obtain their respective fractions. Amino acid analysis was by cation-exchange chromatography using automated amino acid analyser. Antioxidant potential was obtained by spectrophotometric assay using 2, 2-Diphenyl-1-picrylhydrazyl DPPH while elemental and ionic analyses were carried out by atomic absorption spectrophotometry and potentiometric titration, respectively.Results: Proline, alanine, serine, valine, glycine, glutamate and lysine were found in the ethanol fraction while lysine, phenyl alanine, glycine and serine were present in the ethyl acetate fraction but not in the non-polar fractions, n-hexane and chloroform. The ethyl acetate fraction contained more lysine, phenyl alanine, glycine and serine the other leaf fractions. Minimal radical scavenging activity of all the fractions was recorded. The most abundant cations in the extract were potassium and phosphorus (2.16 +/- 0.05 and 1.90 +/- 0.06 mg/g, respectively) while the most abundant anion was phosphate with a concentration of 23.23 +/- 4.61 mg/g for the aqueous leaf fractionConclusion: The study shows that CLL fractions contain variety of amino acids which could promote wound healing, as well as major and minor elemental ions which, as essential body electrolytes, are required for various metabolic processes in the body.
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spelling Amino acid, Antioxidant and Ion Profiles of Carpolobia lutea Leaf (Polygalaceae)Carpolobia leafPolygalaceaeAmino acidAntioxidantIonic profilePurpose: To evaluate the amino acid, antioxidant and ionic profiles of Carpolobia lutea leaf (Polygalaceae) extract (CLL).Methods: The powdered leaf was macerated and subjected to gradient solvent extraction with n-hexane, chloroform, ethyl acetate and ethanol for 72 h to obtain their respective fractions. Amino acid analysis was by cation-exchange chromatography using automated amino acid analyser. Antioxidant potential was obtained by spectrophotometric assay using 2, 2-Diphenyl-1-picrylhydrazyl DPPH while elemental and ionic analyses were carried out by atomic absorption spectrophotometry and potentiometric titration, respectively.Results: Proline, alanine, serine, valine, glycine, glutamate and lysine were found in the ethanol fraction while lysine, phenyl alanine, glycine and serine were present in the ethyl acetate fraction but not in the non-polar fractions, n-hexane and chloroform. The ethyl acetate fraction contained more lysine, phenyl alanine, glycine and serine the other leaf fractions. Minimal radical scavenging activity of all the fractions was recorded. The most abundant cations in the extract were potassium and phosphorus (2.16 +/- 0.05 and 1.90 +/- 0.06 mg/g, respectively) while the most abundant anion was phosphate with a concentration of 23.23 +/- 4.61 mg/g for the aqueous leaf fractionConclusion: The study shows that CLL fractions contain variety of amino acids which could promote wound healing, as well as major and minor elemental ions which, as essential body electrolytes, are required for various metabolic processes in the body.Niger Delta University Postgraduate Fellowship of NigeriaKhana LGA, Rivers State, NigeriaFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Niger Delta Univ, Fac Pharm, Dept Pharmacol & Toxicol, Wilberforce Isl, Bayelsa State, NigeriaSão Paulo State Univ UNESP, Dept Biochem, Inst Chem, BR-14801970 Araraquara, SP, BrazilSão Paulo State Univ UNESP, Inst Chem, Dept Organ Chem, BR-14801970 Araraquara, SP, BrazilSão Paulo State Univ UNESP, Dept Biochem, Inst Chem, BR-14801970 Araraquara, SP, BrazilSão Paulo State Univ UNESP, Inst Chem, Dept Organ Chem, BR-14801970 Araraquara, SP, BrazilPharmacotherapy GroupNiger Delta UnivUniversidade Estadual Paulista (Unesp)Nwidu, Lucky LegbosiCilli, Eduardo Maffud [UNESP]Vilegas, Wagner [UNESP]2014-05-20T14:17:34Z2014-05-20T14:17:34Z2012-10-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article807-813http://dx.doi.org/10.4314/tjpr.v11i5.15Tropical Journal of Pharmaceutical Research. Benin City: Pharmacotherapy Group, v. 11, n. 5, p. 807-813, 2012.1596-5996http://hdl.handle.net/11449/2525910.4314/tjpr.v11i5.15WOS:00031005180001594243467624604160000-0002-4767-09040000-0003-3032-2556Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengTropical Journal of Pharmaceutical Research0.4440,256info:eu-repo/semantics/openAccess2021-10-22T20:56:12Zoai:repositorio.unesp.br:11449/25259Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T21:39:02.128289Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Amino acid, Antioxidant and Ion Profiles of Carpolobia lutea Leaf (Polygalaceae)
title Amino acid, Antioxidant and Ion Profiles of Carpolobia lutea Leaf (Polygalaceae)
spellingShingle Amino acid, Antioxidant and Ion Profiles of Carpolobia lutea Leaf (Polygalaceae)
Nwidu, Lucky Legbosi
Carpolobia leaf
Polygalaceae
Amino acid
Antioxidant
Ionic profile
title_short Amino acid, Antioxidant and Ion Profiles of Carpolobia lutea Leaf (Polygalaceae)
title_full Amino acid, Antioxidant and Ion Profiles of Carpolobia lutea Leaf (Polygalaceae)
title_fullStr Amino acid, Antioxidant and Ion Profiles of Carpolobia lutea Leaf (Polygalaceae)
title_full_unstemmed Amino acid, Antioxidant and Ion Profiles of Carpolobia lutea Leaf (Polygalaceae)
title_sort Amino acid, Antioxidant and Ion Profiles of Carpolobia lutea Leaf (Polygalaceae)
author Nwidu, Lucky Legbosi
author_facet Nwidu, Lucky Legbosi
Cilli, Eduardo Maffud [UNESP]
Vilegas, Wagner [UNESP]
author_role author
author2 Cilli, Eduardo Maffud [UNESP]
Vilegas, Wagner [UNESP]
author2_role author
author
dc.contributor.none.fl_str_mv Niger Delta Univ
Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Nwidu, Lucky Legbosi
Cilli, Eduardo Maffud [UNESP]
Vilegas, Wagner [UNESP]
dc.subject.por.fl_str_mv Carpolobia leaf
Polygalaceae
Amino acid
Antioxidant
Ionic profile
topic Carpolobia leaf
Polygalaceae
Amino acid
Antioxidant
Ionic profile
description Purpose: To evaluate the amino acid, antioxidant and ionic profiles of Carpolobia lutea leaf (Polygalaceae) extract (CLL).Methods: The powdered leaf was macerated and subjected to gradient solvent extraction with n-hexane, chloroform, ethyl acetate and ethanol for 72 h to obtain their respective fractions. Amino acid analysis was by cation-exchange chromatography using automated amino acid analyser. Antioxidant potential was obtained by spectrophotometric assay using 2, 2-Diphenyl-1-picrylhydrazyl DPPH while elemental and ionic analyses were carried out by atomic absorption spectrophotometry and potentiometric titration, respectively.Results: Proline, alanine, serine, valine, glycine, glutamate and lysine were found in the ethanol fraction while lysine, phenyl alanine, glycine and serine were present in the ethyl acetate fraction but not in the non-polar fractions, n-hexane and chloroform. The ethyl acetate fraction contained more lysine, phenyl alanine, glycine and serine the other leaf fractions. Minimal radical scavenging activity of all the fractions was recorded. The most abundant cations in the extract were potassium and phosphorus (2.16 +/- 0.05 and 1.90 +/- 0.06 mg/g, respectively) while the most abundant anion was phosphate with a concentration of 23.23 +/- 4.61 mg/g for the aqueous leaf fractionConclusion: The study shows that CLL fractions contain variety of amino acids which could promote wound healing, as well as major and minor elemental ions which, as essential body electrolytes, are required for various metabolic processes in the body.
publishDate 2012
dc.date.none.fl_str_mv 2012-10-01
2014-05-20T14:17:34Z
2014-05-20T14:17:34Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.4314/tjpr.v11i5.15
Tropical Journal of Pharmaceutical Research. Benin City: Pharmacotherapy Group, v. 11, n. 5, p. 807-813, 2012.
1596-5996
http://hdl.handle.net/11449/25259
10.4314/tjpr.v11i5.15
WOS:000310051800015
9424346762460416
0000-0002-4767-0904
0000-0003-3032-2556
url http://dx.doi.org/10.4314/tjpr.v11i5.15
http://hdl.handle.net/11449/25259
identifier_str_mv Tropical Journal of Pharmaceutical Research. Benin City: Pharmacotherapy Group, v. 11, n. 5, p. 807-813, 2012.
1596-5996
10.4314/tjpr.v11i5.15
WOS:000310051800015
9424346762460416
0000-0002-4767-0904
0000-0003-3032-2556
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Tropical Journal of Pharmaceutical Research
0.444
0,256
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 807-813
dc.publisher.none.fl_str_mv Pharmacotherapy Group
publisher.none.fl_str_mv Pharmacotherapy Group
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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