Whey protein enriched with Stevia rebaudiana fraction restores the pancreatic function of streptozotocin induced diabetic rats
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s13197-020-04799-3 http://hdl.handle.net/11449/208830 |
Resumo: | The study aims to analyse the treatment of whey protein enriched withStevia rebaudianafraction in insulin secretion and its role mitigating streptozotocin-induced hyperglycemia in rats. Thus, diabetic animals were treated with whey protein enriched withS. rebaudianafraction or with only the protein isolate or only the Stevia fraction. Insulin level in plasma was measured by radioimmunoassay and the viability of beta cells was detected by immunohistochemistry. The results showed that diabetic animals treated with whey protein enriched withS. rebaudianafraction had a greater recovery from insulinemia, with plasma levels similar to non-diabetic animals (similar to 0.13 ng/mL). In addition, the same group showed a higher number of insulin-positive pancreatic B cells (similar to 66%) in immunohistochemistry analysis, while the diabetic groups treated with only the fraction of stevia or whey protein showed 38 and 59% of positive cells, respectively. These results show that the treatment may have restored the viability of streptozotocin-injured pancreatic B cells, and consequently increased insulin secretion, suggesting whey protein enriched withS. rebaudianafraction can be used an adjunct/supplement in diabetic treatment. |
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spelling |
Whey protein enriched with Stevia rebaudiana fraction restores the pancreatic function of streptozotocin induced diabetic ratsInsulin secretionReduction of hyperglycemiaDiabetesSupplementationFraction of ethyl acetateThe study aims to analyse the treatment of whey protein enriched withStevia rebaudianafraction in insulin secretion and its role mitigating streptozotocin-induced hyperglycemia in rats. Thus, diabetic animals were treated with whey protein enriched withS. rebaudianafraction or with only the protein isolate or only the Stevia fraction. Insulin level in plasma was measured by radioimmunoassay and the viability of beta cells was detected by immunohistochemistry. The results showed that diabetic animals treated with whey protein enriched withS. rebaudianafraction had a greater recovery from insulinemia, with plasma levels similar to non-diabetic animals (similar to 0.13 ng/mL). In addition, the same group showed a higher number of insulin-positive pancreatic B cells (similar to 66%) in immunohistochemistry analysis, while the diabetic groups treated with only the fraction of stevia or whey protein showed 38 and 59% of positive cells, respectively. These results show that the treatment may have restored the viability of streptozotocin-injured pancreatic B cells, and consequently increased insulin secretion, suggesting whey protein enriched withS. rebaudianafraction can be used an adjunct/supplement in diabetic treatment.Univ Estadual Maringa, Dept Biochem, Maringa, PR, BrazilUniv Estadual Maringa, Dept Biotechnol Genet & Cell Biol, Maringa, PR, BrazilState Univ Sao Paulo, Postgrad Program Sci Human Physiol, Sao Paulo, SP, BrazilUniv North Parana, UNOPAR, Bandeirantes, PR, BrazilUniv Estadual Maringa, Dept Physiol Sci, Maringa, PR, BrazilUniv Estadual Maringa, Dept Morphol Sci, Maringa, PR, BrazilState Univ Sao Paulo, Postgrad Program Sci Human Physiol, Sao Paulo, SP, BrazilSpringerUniversidade Estadual de Maringá (UEM)Universidade Estadual Paulista (Unesp)Univ North ParanaMilani, Paula GimenezPiovan, SilvanoLima, Yago Carvalho [UNESP]Zorzenon, Maria Rosa TrentinRosa, Carlos Vinicius Dalto daPeixoto, Giuliana Maria LedesmaFreitas Mathias, Paulo Cezar deNatali, Maria Raquel MarcalCosta, Silvio Claudio daMareze-Costa, Cecilia Edna2021-06-25T11:21:52Z2021-06-25T11:21:52Z2020-09-25info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article805-810http://dx.doi.org/10.1007/s13197-020-04799-3Journal Of Food Science And Technology-mysore. New Delhi: Springer India, v. 58, n. 2, p. 805-810, 2021.0022-1155http://hdl.handle.net/11449/20883010.1007/s13197-020-04799-3WOS:000572701800002Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal Of Food Science And Technology-mysoreinfo:eu-repo/semantics/openAccess2021-10-23T19:23:14Zoai:repositorio.unesp.br:11449/208830Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T15:07:41.210767Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Whey protein enriched with Stevia rebaudiana fraction restores the pancreatic function of streptozotocin induced diabetic rats |
title |
Whey protein enriched with Stevia rebaudiana fraction restores the pancreatic function of streptozotocin induced diabetic rats |
spellingShingle |
Whey protein enriched with Stevia rebaudiana fraction restores the pancreatic function of streptozotocin induced diabetic rats Milani, Paula Gimenez Insulin secretion Reduction of hyperglycemia Diabetes Supplementation Fraction of ethyl acetate |
title_short |
Whey protein enriched with Stevia rebaudiana fraction restores the pancreatic function of streptozotocin induced diabetic rats |
title_full |
Whey protein enriched with Stevia rebaudiana fraction restores the pancreatic function of streptozotocin induced diabetic rats |
title_fullStr |
Whey protein enriched with Stevia rebaudiana fraction restores the pancreatic function of streptozotocin induced diabetic rats |
title_full_unstemmed |
Whey protein enriched with Stevia rebaudiana fraction restores the pancreatic function of streptozotocin induced diabetic rats |
title_sort |
Whey protein enriched with Stevia rebaudiana fraction restores the pancreatic function of streptozotocin induced diabetic rats |
author |
Milani, Paula Gimenez |
author_facet |
Milani, Paula Gimenez Piovan, Silvano Lima, Yago Carvalho [UNESP] Zorzenon, Maria Rosa Trentin Rosa, Carlos Vinicius Dalto da Peixoto, Giuliana Maria Ledesma Freitas Mathias, Paulo Cezar de Natali, Maria Raquel Marcal Costa, Silvio Claudio da Mareze-Costa, Cecilia Edna |
author_role |
author |
author2 |
Piovan, Silvano Lima, Yago Carvalho [UNESP] Zorzenon, Maria Rosa Trentin Rosa, Carlos Vinicius Dalto da Peixoto, Giuliana Maria Ledesma Freitas Mathias, Paulo Cezar de Natali, Maria Raquel Marcal Costa, Silvio Claudio da Mareze-Costa, Cecilia Edna |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual de Maringá (UEM) Universidade Estadual Paulista (Unesp) Univ North Parana |
dc.contributor.author.fl_str_mv |
Milani, Paula Gimenez Piovan, Silvano Lima, Yago Carvalho [UNESP] Zorzenon, Maria Rosa Trentin Rosa, Carlos Vinicius Dalto da Peixoto, Giuliana Maria Ledesma Freitas Mathias, Paulo Cezar de Natali, Maria Raquel Marcal Costa, Silvio Claudio da Mareze-Costa, Cecilia Edna |
dc.subject.por.fl_str_mv |
Insulin secretion Reduction of hyperglycemia Diabetes Supplementation Fraction of ethyl acetate |
topic |
Insulin secretion Reduction of hyperglycemia Diabetes Supplementation Fraction of ethyl acetate |
description |
The study aims to analyse the treatment of whey protein enriched withStevia rebaudianafraction in insulin secretion and its role mitigating streptozotocin-induced hyperglycemia in rats. Thus, diabetic animals were treated with whey protein enriched withS. rebaudianafraction or with only the protein isolate or only the Stevia fraction. Insulin level in plasma was measured by radioimmunoassay and the viability of beta cells was detected by immunohistochemistry. The results showed that diabetic animals treated with whey protein enriched withS. rebaudianafraction had a greater recovery from insulinemia, with plasma levels similar to non-diabetic animals (similar to 0.13 ng/mL). In addition, the same group showed a higher number of insulin-positive pancreatic B cells (similar to 66%) in immunohistochemistry analysis, while the diabetic groups treated with only the fraction of stevia or whey protein showed 38 and 59% of positive cells, respectively. These results show that the treatment may have restored the viability of streptozotocin-injured pancreatic B cells, and consequently increased insulin secretion, suggesting whey protein enriched withS. rebaudianafraction can be used an adjunct/supplement in diabetic treatment. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-09-25 2021-06-25T11:21:52Z 2021-06-25T11:21:52Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s13197-020-04799-3 Journal Of Food Science And Technology-mysore. New Delhi: Springer India, v. 58, n. 2, p. 805-810, 2021. 0022-1155 http://hdl.handle.net/11449/208830 10.1007/s13197-020-04799-3 WOS:000572701800002 |
url |
http://dx.doi.org/10.1007/s13197-020-04799-3 http://hdl.handle.net/11449/208830 |
identifier_str_mv |
Journal Of Food Science And Technology-mysore. New Delhi: Springer India, v. 58, n. 2, p. 805-810, 2021. 0022-1155 10.1007/s13197-020-04799-3 WOS:000572701800002 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal Of Food Science And Technology-mysore |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
805-810 |
dc.publisher.none.fl_str_mv |
Springer |
publisher.none.fl_str_mv |
Springer |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128465148313600 |