Upregulation of INF-γ, IL-6, and IL-8 expression during replication of turkey coronavirus in nonepithelial cells obtained from Meleagris gallopavo

Detalhes bibliográficos
Autor(a) principal: Cardoso, Tereza Cristina [UNESP]
Data de Publicação: 2021
Outros Autores: Panegossi, Letícia Colin [UNESP], Gameiro, Roberto
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1007/s00705-021-05120-z
http://hdl.handle.net/11449/206440
Resumo: Mesenchymal stromal cells (MSCs) are considered multipotent progenitors with the capacity to differentiate into mesoderm-like cells in many species. The immunosuppressive properties of MSCs are important for downregulating inflammatory responses. Turkey coronavirus (TCoV) is the etiological agent of a poult mortality syndrome that affects intestinal epithelial cells. In this study, poult MSCs were isolated, characterized, and infected with TCoV after in vitro culture. The poult-derived MSCs showed fibroblast-like morphology and the ability to undergo differentiation into mesodermal-derived cells and to support virus replication. Infection with TCoV resulted in cytopathic effects and the loss of cell viability. TCoV antigens and new viral progeny were detected at high levels, as were transcripts of the pro-inflammatory factors INFγ, IL-6, and IL-8. These findings suggest that the cytokine storm phenomenon is not restricted to one genus of the family Coronaviridae and that MSCs cannot always balance the process.
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spelling Upregulation of INF-γ, IL-6, and IL-8 expression during replication of turkey coronavirus in nonepithelial cells obtained from Meleagris gallopavoMesenchymal stromal cells (MSCs) are considered multipotent progenitors with the capacity to differentiate into mesoderm-like cells in many species. The immunosuppressive properties of MSCs are important for downregulating inflammatory responses. Turkey coronavirus (TCoV) is the etiological agent of a poult mortality syndrome that affects intestinal epithelial cells. In this study, poult MSCs were isolated, characterized, and infected with TCoV after in vitro culture. The poult-derived MSCs showed fibroblast-like morphology and the ability to undergo differentiation into mesodermal-derived cells and to support virus replication. Infection with TCoV resulted in cytopathic effects and the loss of cell viability. TCoV antigens and new viral progeny were detected at high levels, as were transcripts of the pro-inflammatory factors INFγ, IL-6, and IL-8. These findings suggest that the cytokine storm phenomenon is not restricted to one genus of the family Coronaviridae and that MSCs cannot always balance the process.Laboratory of Animal Virology and Cell Culture UNESP-University of São Paulo StateLaboratory of Anatomy Histology and Embryology College of Veterinary MedicineDepartamento de Apoio Produção e Saúde Animal Curso de Medicina Veterinária, Rua Clóvis Pestana, 793Laboratory of Animal Virology and Cell Culture UNESP-University of São Paulo StateUniversidade Estadual Paulista (Unesp)College of Veterinary MedicineCurso de Medicina VeterináriaCardoso, Tereza Cristina [UNESP]Panegossi, Letícia Colin [UNESP]Gameiro, Roberto2021-06-25T10:32:06Z2021-06-25T10:32:06Z2021-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1007/s00705-021-05120-zArchives of Virology.1432-87980304-8608http://hdl.handle.net/11449/20644010.1007/s00705-021-05120-z2-s2.0-85107329044Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengArchives of Virologyinfo:eu-repo/semantics/openAccess2021-10-23T04:45:29Zoai:repositorio.unesp.br:11449/206440Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T22:06:34.933673Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Upregulation of INF-γ, IL-6, and IL-8 expression during replication of turkey coronavirus in nonepithelial cells obtained from Meleagris gallopavo
title Upregulation of INF-γ, IL-6, and IL-8 expression during replication of turkey coronavirus in nonepithelial cells obtained from Meleagris gallopavo
spellingShingle Upregulation of INF-γ, IL-6, and IL-8 expression during replication of turkey coronavirus in nonepithelial cells obtained from Meleagris gallopavo
Cardoso, Tereza Cristina [UNESP]
title_short Upregulation of INF-γ, IL-6, and IL-8 expression during replication of turkey coronavirus in nonepithelial cells obtained from Meleagris gallopavo
title_full Upregulation of INF-γ, IL-6, and IL-8 expression during replication of turkey coronavirus in nonepithelial cells obtained from Meleagris gallopavo
title_fullStr Upregulation of INF-γ, IL-6, and IL-8 expression during replication of turkey coronavirus in nonepithelial cells obtained from Meleagris gallopavo
title_full_unstemmed Upregulation of INF-γ, IL-6, and IL-8 expression during replication of turkey coronavirus in nonepithelial cells obtained from Meleagris gallopavo
title_sort Upregulation of INF-γ, IL-6, and IL-8 expression during replication of turkey coronavirus in nonepithelial cells obtained from Meleagris gallopavo
author Cardoso, Tereza Cristina [UNESP]
author_facet Cardoso, Tereza Cristina [UNESP]
Panegossi, Letícia Colin [UNESP]
Gameiro, Roberto
author_role author
author2 Panegossi, Letícia Colin [UNESP]
Gameiro, Roberto
author2_role author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
College of Veterinary Medicine
Curso de Medicina Veterinária
dc.contributor.author.fl_str_mv Cardoso, Tereza Cristina [UNESP]
Panegossi, Letícia Colin [UNESP]
Gameiro, Roberto
description Mesenchymal stromal cells (MSCs) are considered multipotent progenitors with the capacity to differentiate into mesoderm-like cells in many species. The immunosuppressive properties of MSCs are important for downregulating inflammatory responses. Turkey coronavirus (TCoV) is the etiological agent of a poult mortality syndrome that affects intestinal epithelial cells. In this study, poult MSCs were isolated, characterized, and infected with TCoV after in vitro culture. The poult-derived MSCs showed fibroblast-like morphology and the ability to undergo differentiation into mesodermal-derived cells and to support virus replication. Infection with TCoV resulted in cytopathic effects and the loss of cell viability. TCoV antigens and new viral progeny were detected at high levels, as were transcripts of the pro-inflammatory factors INFγ, IL-6, and IL-8. These findings suggest that the cytokine storm phenomenon is not restricted to one genus of the family Coronaviridae and that MSCs cannot always balance the process.
publishDate 2021
dc.date.none.fl_str_mv 2021-06-25T10:32:06Z
2021-06-25T10:32:06Z
2021-01-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1007/s00705-021-05120-z
Archives of Virology.
1432-8798
0304-8608
http://hdl.handle.net/11449/206440
10.1007/s00705-021-05120-z
2-s2.0-85107329044
url http://dx.doi.org/10.1007/s00705-021-05120-z
http://hdl.handle.net/11449/206440
identifier_str_mv Archives of Virology.
1432-8798
0304-8608
10.1007/s00705-021-05120-z
2-s2.0-85107329044
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Archives of Virology
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
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