Molecular characterization of intestinal protozoa in two poor communities in the State of São Paulo, Brazil
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://www.parasitesandvectors.com/content/8/1/103 http://hdl.handle.net/11449/128648 |
Resumo: | Background: Several species of protozoa cause acute or chronic gastroenteritis in humans, worldwide. The burden of disease is particularly high among children living in developing areas of the world, where transmission is favored by lower hygienic standards and scarce availability of safe water. However, asymptomatic infection and polyparasitism are also commonly observed in poor settings. Here, we investigated the prevalence of intestinal protozoa in two small fishing villages, Porto Said (PS) and Santa Maria da Serra (SM), situated along the river Tiete in the State of Sao Paolo, Brazil. The villages lack basic public infrastructure and services, such as roads, public water supply, electricity and public health services.Methods: Multiple fecal samples were collected from 88 individuals in PS and from 38 individuals in SM, who were asymptomatic at the time of sampling and had no recent history of diarrheal disease. To gain insights into potential transmission routes, 49 dog fecal samples (38 from PS and 11 from SM) and 28 river water samples were also collected. All samples were tested by microscopy and PCR was used to genotype Giardia duodenalis, Blastocystis sp., Dientamoeba fragilis and Cryptosporidium spp.Results: By molecular methods, the most common human parasite was Blastocystis sp. (prevalence, 45% in PS and 71% in SM), followed by D. fragilis (13.6% in PS, and 18.4% in SM) and G. duodenalis (18.2% in PS and 7.9% in SM); Cryptosporidium spp. were not detected. Sequence analysis revealed large genetic variation among Blastocystis samples, with subtypes (STs) 1 and 3 being predominant, and with the notable absence of ST4. Among G. duodenalis samples, assemblages A and B were detected in humans, whereas assemblages A, C and D were found in dogs. Finally, all D. fragilis samples from humans were genotype 1. A single dog was found infected with Cryptosporidium canis. River water samples were negative for the investigated parasites.Conclusions: This study showed a high carriage of intestinal parasites in asymptomatic individuals from two poor Brazilian villages, and highlighted a large genetic variability of Blastocystis spp. and G. duodenalis. |
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Molecular characterization of intestinal protozoa in two poor communities in the State of São Paulo, BrazilBrazilIntestinal protozoaHumansDogsRiver waterMolecular typingBackground: Several species of protozoa cause acute or chronic gastroenteritis in humans, worldwide. The burden of disease is particularly high among children living in developing areas of the world, where transmission is favored by lower hygienic standards and scarce availability of safe water. However, asymptomatic infection and polyparasitism are also commonly observed in poor settings. Here, we investigated the prevalence of intestinal protozoa in two small fishing villages, Porto Said (PS) and Santa Maria da Serra (SM), situated along the river Tiete in the State of Sao Paolo, Brazil. The villages lack basic public infrastructure and services, such as roads, public water supply, electricity and public health services.Methods: Multiple fecal samples were collected from 88 individuals in PS and from 38 individuals in SM, who were asymptomatic at the time of sampling and had no recent history of diarrheal disease. To gain insights into potential transmission routes, 49 dog fecal samples (38 from PS and 11 from SM) and 28 river water samples were also collected. All samples were tested by microscopy and PCR was used to genotype Giardia duodenalis, Blastocystis sp., Dientamoeba fragilis and Cryptosporidium spp.Results: By molecular methods, the most common human parasite was Blastocystis sp. (prevalence, 45% in PS and 71% in SM), followed by D. fragilis (13.6% in PS, and 18.4% in SM) and G. duodenalis (18.2% in PS and 7.9% in SM); Cryptosporidium spp. were not detected. Sequence analysis revealed large genetic variation among Blastocystis samples, with subtypes (STs) 1 and 3 being predominant, and with the notable absence of ST4. Among G. duodenalis samples, assemblages A and B were detected in humans, whereas assemblages A, C and D were found in dogs. Finally, all D. fragilis samples from humans were genotype 1. A single dog was found infected with Cryptosporidium canis. River water samples were negative for the investigated parasites.Conclusions: This study showed a high carriage of intestinal parasites in asymptomatic individuals from two poor Brazilian villages, and highlighted a large genetic variability of Blastocystis spp. and G. duodenalis.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Sao Paulo State Univ, UNESP, Inst Biosci, Dept Parasitol, BR-18618970 Botucatu, SP, BrazilUniv Estadual Campinas, Inst Biol, Dept Anim Biol, BR-13083970 Campinas, SP, BrazilIst Super Sanita, Dept Infect Parasit &Immunomediated Dis, I-00161 Rome, ItalySao Paulo State Univ, UNESP, Inst Biosci, Dept Parasitol, BR-18618970 Botucatu, SP, BrazilFAPESP: 2011/52100-3Biomed Central LtdUniversidade Estadual Paulista (Unesp)Universidade Estadual de Campinas (UNICAMP)Ist Super SanitaDavid, Erica Boarato [UNESP]Guimaraes, Semiramis [UNESP]Oliveira, Ana Paula de [UNESP]Goulart de Oliveira-Sequeira, Teresa Cristina [UNESP]Bittencourt, Gabriela Nogueira [UNESP]Moraes Nardi, Ana RitaMartins Ribolla, Paulo Eduardo [UNESP]Bueno Franco, Regina MauraBranco, NilsonTosini, FabioBella, AntoninoPozio, EdoardoCaccio, Simone M.2015-10-21T13:11:52Z2015-10-21T13:11:52Z2015-02-15info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article12application/pdfhttp://www.parasitesandvectors.com/content/8/1/103Parasites &vectors, v. 8, 12 p., 2015.1756-3305http://hdl.handle.net/11449/12864810.1186/s13071-015-0714-8WOS:000349703400001WOS000349703400001.pdf3577149748456880Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengParasites &vectors3.1631,702info:eu-repo/semantics/openAccess2024-01-23T07:08:34Zoai:repositorio.unesp.br:11449/128648Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T23:46:24.609219Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Molecular characterization of intestinal protozoa in two poor communities in the State of São Paulo, Brazil |
title |
Molecular characterization of intestinal protozoa in two poor communities in the State of São Paulo, Brazil |
spellingShingle |
Molecular characterization of intestinal protozoa in two poor communities in the State of São Paulo, Brazil David, Erica Boarato [UNESP] Brazil Intestinal protozoa Humans Dogs River water Molecular typing |
title_short |
Molecular characterization of intestinal protozoa in two poor communities in the State of São Paulo, Brazil |
title_full |
Molecular characterization of intestinal protozoa in two poor communities in the State of São Paulo, Brazil |
title_fullStr |
Molecular characterization of intestinal protozoa in two poor communities in the State of São Paulo, Brazil |
title_full_unstemmed |
Molecular characterization of intestinal protozoa in two poor communities in the State of São Paulo, Brazil |
title_sort |
Molecular characterization of intestinal protozoa in two poor communities in the State of São Paulo, Brazil |
author |
David, Erica Boarato [UNESP] |
author_facet |
David, Erica Boarato [UNESP] Guimaraes, Semiramis [UNESP] Oliveira, Ana Paula de [UNESP] Goulart de Oliveira-Sequeira, Teresa Cristina [UNESP] Bittencourt, Gabriela Nogueira [UNESP] Moraes Nardi, Ana Rita Martins Ribolla, Paulo Eduardo [UNESP] Bueno Franco, Regina Maura Branco, Nilson Tosini, Fabio Bella, Antonino Pozio, Edoardo Caccio, Simone M. |
author_role |
author |
author2 |
Guimaraes, Semiramis [UNESP] Oliveira, Ana Paula de [UNESP] Goulart de Oliveira-Sequeira, Teresa Cristina [UNESP] Bittencourt, Gabriela Nogueira [UNESP] Moraes Nardi, Ana Rita Martins Ribolla, Paulo Eduardo [UNESP] Bueno Franco, Regina Maura Branco, Nilson Tosini, Fabio Bella, Antonino Pozio, Edoardo Caccio, Simone M. |
author2_role |
author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade Estadual de Campinas (UNICAMP) Ist Super Sanita |
dc.contributor.author.fl_str_mv |
David, Erica Boarato [UNESP] Guimaraes, Semiramis [UNESP] Oliveira, Ana Paula de [UNESP] Goulart de Oliveira-Sequeira, Teresa Cristina [UNESP] Bittencourt, Gabriela Nogueira [UNESP] Moraes Nardi, Ana Rita Martins Ribolla, Paulo Eduardo [UNESP] Bueno Franco, Regina Maura Branco, Nilson Tosini, Fabio Bella, Antonino Pozio, Edoardo Caccio, Simone M. |
dc.subject.por.fl_str_mv |
Brazil Intestinal protozoa Humans Dogs River water Molecular typing |
topic |
Brazil Intestinal protozoa Humans Dogs River water Molecular typing |
description |
Background: Several species of protozoa cause acute or chronic gastroenteritis in humans, worldwide. The burden of disease is particularly high among children living in developing areas of the world, where transmission is favored by lower hygienic standards and scarce availability of safe water. However, asymptomatic infection and polyparasitism are also commonly observed in poor settings. Here, we investigated the prevalence of intestinal protozoa in two small fishing villages, Porto Said (PS) and Santa Maria da Serra (SM), situated along the river Tiete in the State of Sao Paolo, Brazil. The villages lack basic public infrastructure and services, such as roads, public water supply, electricity and public health services.Methods: Multiple fecal samples were collected from 88 individuals in PS and from 38 individuals in SM, who were asymptomatic at the time of sampling and had no recent history of diarrheal disease. To gain insights into potential transmission routes, 49 dog fecal samples (38 from PS and 11 from SM) and 28 river water samples were also collected. All samples were tested by microscopy and PCR was used to genotype Giardia duodenalis, Blastocystis sp., Dientamoeba fragilis and Cryptosporidium spp.Results: By molecular methods, the most common human parasite was Blastocystis sp. (prevalence, 45% in PS and 71% in SM), followed by D. fragilis (13.6% in PS, and 18.4% in SM) and G. duodenalis (18.2% in PS and 7.9% in SM); Cryptosporidium spp. were not detected. Sequence analysis revealed large genetic variation among Blastocystis samples, with subtypes (STs) 1 and 3 being predominant, and with the notable absence of ST4. Among G. duodenalis samples, assemblages A and B were detected in humans, whereas assemblages A, C and D were found in dogs. Finally, all D. fragilis samples from humans were genotype 1. A single dog was found infected with Cryptosporidium canis. River water samples were negative for the investigated parasites.Conclusions: This study showed a high carriage of intestinal parasites in asymptomatic individuals from two poor Brazilian villages, and highlighted a large genetic variability of Blastocystis spp. and G. duodenalis. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-10-21T13:11:52Z 2015-10-21T13:11:52Z 2015-02-15 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.parasitesandvectors.com/content/8/1/103 Parasites &vectors, v. 8, 12 p., 2015. 1756-3305 http://hdl.handle.net/11449/128648 10.1186/s13071-015-0714-8 WOS:000349703400001 WOS000349703400001.pdf 3577149748456880 |
url |
http://www.parasitesandvectors.com/content/8/1/103 http://hdl.handle.net/11449/128648 |
identifier_str_mv |
Parasites &vectors, v. 8, 12 p., 2015. 1756-3305 10.1186/s13071-015-0714-8 WOS:000349703400001 WOS000349703400001.pdf 3577149748456880 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Parasites &vectors 3.163 1,702 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
12 application/pdf |
dc.publisher.none.fl_str_mv |
Biomed Central Ltd |
publisher.none.fl_str_mv |
Biomed Central Ltd |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808129550147649536 |