Molecular characterization of intestinal protozoa in two poor communities in the State of São Paulo, Brazil

Detalhes bibliográficos
Autor(a) principal: David, Erica Boarato [UNESP]
Data de Publicação: 2015
Outros Autores: Guimaraes, Semiramis [UNESP], Oliveira, Ana Paula de [UNESP], Goulart de Oliveira-Sequeira, Teresa Cristina [UNESP], Bittencourt, Gabriela Nogueira [UNESP], Moraes Nardi, Ana Rita, Martins Ribolla, Paulo Eduardo [UNESP], Bueno Franco, Regina Maura, Branco, Nilson, Tosini, Fabio, Bella, Antonino, Pozio, Edoardo, Caccio, Simone M.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://www.parasitesandvectors.com/content/8/1/103
http://hdl.handle.net/11449/128648
Resumo: Background: Several species of protozoa cause acute or chronic gastroenteritis in humans, worldwide. The burden of disease is particularly high among children living in developing areas of the world, where transmission is favored by lower hygienic standards and scarce availability of safe water. However, asymptomatic infection and polyparasitism are also commonly observed in poor settings. Here, we investigated the prevalence of intestinal protozoa in two small fishing villages, Porto Said (PS) and Santa Maria da Serra (SM), situated along the river Tiete in the State of Sao Paolo, Brazil. The villages lack basic public infrastructure and services, such as roads, public water supply, electricity and public health services.Methods: Multiple fecal samples were collected from 88 individuals in PS and from 38 individuals in SM, who were asymptomatic at the time of sampling and had no recent history of diarrheal disease. To gain insights into potential transmission routes, 49 dog fecal samples (38 from PS and 11 from SM) and 28 river water samples were also collected. All samples were tested by microscopy and PCR was used to genotype Giardia duodenalis, Blastocystis sp., Dientamoeba fragilis and Cryptosporidium spp.Results: By molecular methods, the most common human parasite was Blastocystis sp. (prevalence, 45% in PS and 71% in SM), followed by D. fragilis (13.6% in PS, and 18.4% in SM) and G. duodenalis (18.2% in PS and 7.9% in SM); Cryptosporidium spp. were not detected. Sequence analysis revealed large genetic variation among Blastocystis samples, with subtypes (STs) 1 and 3 being predominant, and with the notable absence of ST4. Among G. duodenalis samples, assemblages A and B were detected in humans, whereas assemblages A, C and D were found in dogs. Finally, all D. fragilis samples from humans were genotype 1. A single dog was found infected with Cryptosporidium canis. River water samples were negative for the investigated parasites.Conclusions: This study showed a high carriage of intestinal parasites in asymptomatic individuals from two poor Brazilian villages, and highlighted a large genetic variability of Blastocystis spp. and G. duodenalis.
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spelling Molecular characterization of intestinal protozoa in two poor communities in the State of São Paulo, BrazilBrazilIntestinal protozoaHumansDogsRiver waterMolecular typingBackground: Several species of protozoa cause acute or chronic gastroenteritis in humans, worldwide. The burden of disease is particularly high among children living in developing areas of the world, where transmission is favored by lower hygienic standards and scarce availability of safe water. However, asymptomatic infection and polyparasitism are also commonly observed in poor settings. Here, we investigated the prevalence of intestinal protozoa in two small fishing villages, Porto Said (PS) and Santa Maria da Serra (SM), situated along the river Tiete in the State of Sao Paolo, Brazil. The villages lack basic public infrastructure and services, such as roads, public water supply, electricity and public health services.Methods: Multiple fecal samples were collected from 88 individuals in PS and from 38 individuals in SM, who were asymptomatic at the time of sampling and had no recent history of diarrheal disease. To gain insights into potential transmission routes, 49 dog fecal samples (38 from PS and 11 from SM) and 28 river water samples were also collected. All samples were tested by microscopy and PCR was used to genotype Giardia duodenalis, Blastocystis sp., Dientamoeba fragilis and Cryptosporidium spp.Results: By molecular methods, the most common human parasite was Blastocystis sp. (prevalence, 45% in PS and 71% in SM), followed by D. fragilis (13.6% in PS, and 18.4% in SM) and G. duodenalis (18.2% in PS and 7.9% in SM); Cryptosporidium spp. were not detected. Sequence analysis revealed large genetic variation among Blastocystis samples, with subtypes (STs) 1 and 3 being predominant, and with the notable absence of ST4. Among G. duodenalis samples, assemblages A and B were detected in humans, whereas assemblages A, C and D were found in dogs. Finally, all D. fragilis samples from humans were genotype 1. A single dog was found infected with Cryptosporidium canis. River water samples were negative for the investigated parasites.Conclusions: This study showed a high carriage of intestinal parasites in asymptomatic individuals from two poor Brazilian villages, and highlighted a large genetic variability of Blastocystis spp. and G. duodenalis.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Sao Paulo State Univ, UNESP, Inst Biosci, Dept Parasitol, BR-18618970 Botucatu, SP, BrazilUniv Estadual Campinas, Inst Biol, Dept Anim Biol, BR-13083970 Campinas, SP, BrazilIst Super Sanita, Dept Infect Parasit &Immunomediated Dis, I-00161 Rome, ItalySao Paulo State Univ, UNESP, Inst Biosci, Dept Parasitol, BR-18618970 Botucatu, SP, BrazilFAPESP: 2011/52100-3Biomed Central LtdUniversidade Estadual Paulista (Unesp)Universidade Estadual de Campinas (UNICAMP)Ist Super SanitaDavid, Erica Boarato [UNESP]Guimaraes, Semiramis [UNESP]Oliveira, Ana Paula de [UNESP]Goulart de Oliveira-Sequeira, Teresa Cristina [UNESP]Bittencourt, Gabriela Nogueira [UNESP]Moraes Nardi, Ana RitaMartins Ribolla, Paulo Eduardo [UNESP]Bueno Franco, Regina MauraBranco, NilsonTosini, FabioBella, AntoninoPozio, EdoardoCaccio, Simone M.2015-10-21T13:11:52Z2015-10-21T13:11:52Z2015-02-15info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article12application/pdfhttp://www.parasitesandvectors.com/content/8/1/103Parasites &vectors, v. 8, 12 p., 2015.1756-3305http://hdl.handle.net/11449/12864810.1186/s13071-015-0714-8WOS:000349703400001WOS000349703400001.pdf3577149748456880Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengParasites &vectors3.1631,702info:eu-repo/semantics/openAccess2024-01-23T07:08:34Zoai:repositorio.unesp.br:11449/128648Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T23:46:24.609219Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Molecular characterization of intestinal protozoa in two poor communities in the State of São Paulo, Brazil
title Molecular characterization of intestinal protozoa in two poor communities in the State of São Paulo, Brazil
spellingShingle Molecular characterization of intestinal protozoa in two poor communities in the State of São Paulo, Brazil
David, Erica Boarato [UNESP]
Brazil
Intestinal protozoa
Humans
Dogs
River water
Molecular typing
title_short Molecular characterization of intestinal protozoa in two poor communities in the State of São Paulo, Brazil
title_full Molecular characterization of intestinal protozoa in two poor communities in the State of São Paulo, Brazil
title_fullStr Molecular characterization of intestinal protozoa in two poor communities in the State of São Paulo, Brazil
title_full_unstemmed Molecular characterization of intestinal protozoa in two poor communities in the State of São Paulo, Brazil
title_sort Molecular characterization of intestinal protozoa in two poor communities in the State of São Paulo, Brazil
author David, Erica Boarato [UNESP]
author_facet David, Erica Boarato [UNESP]
Guimaraes, Semiramis [UNESP]
Oliveira, Ana Paula de [UNESP]
Goulart de Oliveira-Sequeira, Teresa Cristina [UNESP]
Bittencourt, Gabriela Nogueira [UNESP]
Moraes Nardi, Ana Rita
Martins Ribolla, Paulo Eduardo [UNESP]
Bueno Franco, Regina Maura
Branco, Nilson
Tosini, Fabio
Bella, Antonino
Pozio, Edoardo
Caccio, Simone M.
author_role author
author2 Guimaraes, Semiramis [UNESP]
Oliveira, Ana Paula de [UNESP]
Goulart de Oliveira-Sequeira, Teresa Cristina [UNESP]
Bittencourt, Gabriela Nogueira [UNESP]
Moraes Nardi, Ana Rita
Martins Ribolla, Paulo Eduardo [UNESP]
Bueno Franco, Regina Maura
Branco, Nilson
Tosini, Fabio
Bella, Antonino
Pozio, Edoardo
Caccio, Simone M.
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Universidade Estadual de Campinas (UNICAMP)
Ist Super Sanita
dc.contributor.author.fl_str_mv David, Erica Boarato [UNESP]
Guimaraes, Semiramis [UNESP]
Oliveira, Ana Paula de [UNESP]
Goulart de Oliveira-Sequeira, Teresa Cristina [UNESP]
Bittencourt, Gabriela Nogueira [UNESP]
Moraes Nardi, Ana Rita
Martins Ribolla, Paulo Eduardo [UNESP]
Bueno Franco, Regina Maura
Branco, Nilson
Tosini, Fabio
Bella, Antonino
Pozio, Edoardo
Caccio, Simone M.
dc.subject.por.fl_str_mv Brazil
Intestinal protozoa
Humans
Dogs
River water
Molecular typing
topic Brazil
Intestinal protozoa
Humans
Dogs
River water
Molecular typing
description Background: Several species of protozoa cause acute or chronic gastroenteritis in humans, worldwide. The burden of disease is particularly high among children living in developing areas of the world, where transmission is favored by lower hygienic standards and scarce availability of safe water. However, asymptomatic infection and polyparasitism are also commonly observed in poor settings. Here, we investigated the prevalence of intestinal protozoa in two small fishing villages, Porto Said (PS) and Santa Maria da Serra (SM), situated along the river Tiete in the State of Sao Paolo, Brazil. The villages lack basic public infrastructure and services, such as roads, public water supply, electricity and public health services.Methods: Multiple fecal samples were collected from 88 individuals in PS and from 38 individuals in SM, who were asymptomatic at the time of sampling and had no recent history of diarrheal disease. To gain insights into potential transmission routes, 49 dog fecal samples (38 from PS and 11 from SM) and 28 river water samples were also collected. All samples were tested by microscopy and PCR was used to genotype Giardia duodenalis, Blastocystis sp., Dientamoeba fragilis and Cryptosporidium spp.Results: By molecular methods, the most common human parasite was Blastocystis sp. (prevalence, 45% in PS and 71% in SM), followed by D. fragilis (13.6% in PS, and 18.4% in SM) and G. duodenalis (18.2% in PS and 7.9% in SM); Cryptosporidium spp. were not detected. Sequence analysis revealed large genetic variation among Blastocystis samples, with subtypes (STs) 1 and 3 being predominant, and with the notable absence of ST4. Among G. duodenalis samples, assemblages A and B were detected in humans, whereas assemblages A, C and D were found in dogs. Finally, all D. fragilis samples from humans were genotype 1. A single dog was found infected with Cryptosporidium canis. River water samples were negative for the investigated parasites.Conclusions: This study showed a high carriage of intestinal parasites in asymptomatic individuals from two poor Brazilian villages, and highlighted a large genetic variability of Blastocystis spp. and G. duodenalis.
publishDate 2015
dc.date.none.fl_str_mv 2015-10-21T13:11:52Z
2015-10-21T13:11:52Z
2015-02-15
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.parasitesandvectors.com/content/8/1/103
Parasites &vectors, v. 8, 12 p., 2015.
1756-3305
http://hdl.handle.net/11449/128648
10.1186/s13071-015-0714-8
WOS:000349703400001
WOS000349703400001.pdf
3577149748456880
url http://www.parasitesandvectors.com/content/8/1/103
http://hdl.handle.net/11449/128648
identifier_str_mv Parasites &vectors, v. 8, 12 p., 2015.
1756-3305
10.1186/s13071-015-0714-8
WOS:000349703400001
WOS000349703400001.pdf
3577149748456880
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Parasites &vectors
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dc.publisher.none.fl_str_mv Biomed Central Ltd
publisher.none.fl_str_mv Biomed Central Ltd
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
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instname_str Universidade Estadual Paulista (UNESP)
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repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
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