Electrostatic immobilization of antimicrobial peptides on polyethylenimine and their antibacterial effect against Staphylococcus epidermidis
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.colsurfb.2018.02.002 http://hdl.handle.net/11449/164098 |
Resumo: | Staphylococcus epidermidis is a gram-positive bacterium, and one of the most prevalent causes of nosocomial infections due to its strong ability to form biofilms on catheters and surgical implants. Here we explore the antimicrobial properties of Tet-124 peptides, which are part of the innate defense against different multicellular organisms in nature. Two different Tet-124 peptides were immobilized on a polyethylenimine (PEI) film to determine their impact on the antimicrobial properties: KLWWMIRRW (Tet-124), which contains only natural amino acids, and KLWWMIRRWG-(F-Br)-G (F-Br- 4-Bromophenylalanine), a modified Tet-124 sequence with the addition of an unnatural amino acid. The immobilization was obtained as a result of the electrostatic interaction between PEI amino groups and the C-terminal carboxylic groups of tryptophan and glycine amino acids of Tet-124 and Tet-124-Br peptides, respectively. The process was monitored and studied by water contact angle, Atomic Force Microscopy (AFM), X-ray Photoelectron Spectroscopy (XPS) and Quartz Crystal Microbalance with Dissipation (QCM-D) measurements. The antibacterial effect of our samples against S. epidermis was evaluated by the spread plate counting method, and cytotoxicity was tested using fibroblast cultures. Our results indicate the feasibility to immobilize electrostatically both Tet-124 peptides for biomedical applications. (C) 2018 Elsevier B.V. All rights reserved. |
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Electrostatic immobilization of antimicrobial peptides on polyethylenimine and their antibacterial effect against Staphylococcus epidermidisElectrostatic immobilizationAntimicrobial peptidesStaphylococcus epidermidisStaphylococcus epidermidis is a gram-positive bacterium, and one of the most prevalent causes of nosocomial infections due to its strong ability to form biofilms on catheters and surgical implants. Here we explore the antimicrobial properties of Tet-124 peptides, which are part of the innate defense against different multicellular organisms in nature. Two different Tet-124 peptides were immobilized on a polyethylenimine (PEI) film to determine their impact on the antimicrobial properties: KLWWMIRRW (Tet-124), which contains only natural amino acids, and KLWWMIRRWG-(F-Br)-G (F-Br- 4-Bromophenylalanine), a modified Tet-124 sequence with the addition of an unnatural amino acid. The immobilization was obtained as a result of the electrostatic interaction between PEI amino groups and the C-terminal carboxylic groups of tryptophan and glycine amino acids of Tet-124 and Tet-124-Br peptides, respectively. The process was monitored and studied by water contact angle, Atomic Force Microscopy (AFM), X-ray Photoelectron Spectroscopy (XPS) and Quartz Crystal Microbalance with Dissipation (QCM-D) measurements. The antibacterial effect of our samples against S. epidermis was evaluated by the spread plate counting method, and cytotoxicity was tested using fibroblast cultures. Our results indicate the feasibility to immobilize electrostatically both Tet-124 peptides for biomedical applications. (C) 2018 Elsevier B.V. All rights reserved.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Univ Estadual Campinas, Inst Fis Gleb Wataghin, Dept Fis Aplicada, BR-13083859 Campinas, SP, BrazilUniv Catolica Temuco, Fac Ingn, Dept Ciencias Matemat & Fis, Temuco 4813302, ChileUniv Catolica Temuco, Fac Ingn, Nucleo Invest Bioprod & Mat Avanzados BioMa, Temuco 4781312, ChileCtr Natl Alta Tecnol, Lab Nacl Nanotecnol, San Jose 11741200, Costa RicaUniv Estadual Campinas, Fac Ciencias Farmaceut, BR-13083871 Campinas, SP, BrazilFraunhofer Inst Mfg Technol & Adv Mat, D-28359 Bremen, GermanyUniv Estadual Paulista, Fac Ciencias, Dept Fis, BR-17033360 Bauru, BrazilUniv Estadual Paulista, Fac Ciencias, Dept Fis, BR-17033360 Bauru, BrazilFAPESP: 2013/14888-3FAPESP: 2015/16611-4Elsevier B.V.Universidade Estadual de Campinas (UNICAMP)Univ Catolica TemucoCtr Natl Alta TecnolFraunhofer Inst Mfg Technol & Adv MatUniversidade Estadual Paulista (Unesp)Hernandez-Montelongo, J.Corrales Urena, Y. R.Machado, D.Lancelloti, M.Pinheiro, M. P.Rischka, K.Lisboa-Filho, P. N. [UNESP]Cotta, M. A.2018-11-26T17:49:06Z2018-11-26T17:49:06Z2018-04-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article370-378application/pdfhttp://dx.doi.org/10.1016/j.colsurfb.2018.02.002Colloids And Surfaces B-biointerfaces. Amsterdam: Elsevier Science Bv, v. 164, p. 370-378, 2018.0927-7765http://hdl.handle.net/11449/16409810.1016/j.colsurfb.2018.02.002WOS:000429762100043WOS000429762100043.pdf13538624145320050000-0002-7734-4069Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengColloids And Surfaces B-biointerfaces1,071info:eu-repo/semantics/openAccess2024-04-25T17:39:23Zoai:repositorio.unesp.br:11449/164098Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-04-25T17:39:23Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Electrostatic immobilization of antimicrobial peptides on polyethylenimine and their antibacterial effect against Staphylococcus epidermidis |
title |
Electrostatic immobilization of antimicrobial peptides on polyethylenimine and their antibacterial effect against Staphylococcus epidermidis |
spellingShingle |
Electrostatic immobilization of antimicrobial peptides on polyethylenimine and their antibacterial effect against Staphylococcus epidermidis Hernandez-Montelongo, J. Electrostatic immobilization Antimicrobial peptides Staphylococcus epidermidis |
title_short |
Electrostatic immobilization of antimicrobial peptides on polyethylenimine and their antibacterial effect against Staphylococcus epidermidis |
title_full |
Electrostatic immobilization of antimicrobial peptides on polyethylenimine and their antibacterial effect against Staphylococcus epidermidis |
title_fullStr |
Electrostatic immobilization of antimicrobial peptides on polyethylenimine and their antibacterial effect against Staphylococcus epidermidis |
title_full_unstemmed |
Electrostatic immobilization of antimicrobial peptides on polyethylenimine and their antibacterial effect against Staphylococcus epidermidis |
title_sort |
Electrostatic immobilization of antimicrobial peptides on polyethylenimine and their antibacterial effect against Staphylococcus epidermidis |
author |
Hernandez-Montelongo, J. |
author_facet |
Hernandez-Montelongo, J. Corrales Urena, Y. R. Machado, D. Lancelloti, M. Pinheiro, M. P. Rischka, K. Lisboa-Filho, P. N. [UNESP] Cotta, M. A. |
author_role |
author |
author2 |
Corrales Urena, Y. R. Machado, D. Lancelloti, M. Pinheiro, M. P. Rischka, K. Lisboa-Filho, P. N. [UNESP] Cotta, M. A. |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual de Campinas (UNICAMP) Univ Catolica Temuco Ctr Natl Alta Tecnol Fraunhofer Inst Mfg Technol & Adv Mat Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Hernandez-Montelongo, J. Corrales Urena, Y. R. Machado, D. Lancelloti, M. Pinheiro, M. P. Rischka, K. Lisboa-Filho, P. N. [UNESP] Cotta, M. A. |
dc.subject.por.fl_str_mv |
Electrostatic immobilization Antimicrobial peptides Staphylococcus epidermidis |
topic |
Electrostatic immobilization Antimicrobial peptides Staphylococcus epidermidis |
description |
Staphylococcus epidermidis is a gram-positive bacterium, and one of the most prevalent causes of nosocomial infections due to its strong ability to form biofilms on catheters and surgical implants. Here we explore the antimicrobial properties of Tet-124 peptides, which are part of the innate defense against different multicellular organisms in nature. Two different Tet-124 peptides were immobilized on a polyethylenimine (PEI) film to determine their impact on the antimicrobial properties: KLWWMIRRW (Tet-124), which contains only natural amino acids, and KLWWMIRRWG-(F-Br)-G (F-Br- 4-Bromophenylalanine), a modified Tet-124 sequence with the addition of an unnatural amino acid. The immobilization was obtained as a result of the electrostatic interaction between PEI amino groups and the C-terminal carboxylic groups of tryptophan and glycine amino acids of Tet-124 and Tet-124-Br peptides, respectively. The process was monitored and studied by water contact angle, Atomic Force Microscopy (AFM), X-ray Photoelectron Spectroscopy (XPS) and Quartz Crystal Microbalance with Dissipation (QCM-D) measurements. The antibacterial effect of our samples against S. epidermis was evaluated by the spread plate counting method, and cytotoxicity was tested using fibroblast cultures. Our results indicate the feasibility to immobilize electrostatically both Tet-124 peptides for biomedical applications. (C) 2018 Elsevier B.V. All rights reserved. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-11-26T17:49:06Z 2018-11-26T17:49:06Z 2018-04-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.colsurfb.2018.02.002 Colloids And Surfaces B-biointerfaces. Amsterdam: Elsevier Science Bv, v. 164, p. 370-378, 2018. 0927-7765 http://hdl.handle.net/11449/164098 10.1016/j.colsurfb.2018.02.002 WOS:000429762100043 WOS000429762100043.pdf 1353862414532005 0000-0002-7734-4069 |
url |
http://dx.doi.org/10.1016/j.colsurfb.2018.02.002 http://hdl.handle.net/11449/164098 |
identifier_str_mv |
Colloids And Surfaces B-biointerfaces. Amsterdam: Elsevier Science Bv, v. 164, p. 370-378, 2018. 0927-7765 10.1016/j.colsurfb.2018.02.002 WOS:000429762100043 WOS000429762100043.pdf 1353862414532005 0000-0002-7734-4069 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Colloids And Surfaces B-biointerfaces 1,071 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
370-378 application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier B.V. |
publisher.none.fl_str_mv |
Elsevier B.V. |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799964492139855872 |