Antifungal compounds with anticancer potential from Trichoderma sp. P8BDA1F1, an endophytic fungus from Begonia venosa
Autor(a) principal: | |
---|---|
Data de Publicação: | 2020 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s42770-020-00270-9 http://hdl.handle.net/11449/200359 |
Resumo: | Fungi in the genus Trichoderma are notorious producers of secondary metabolites with diverse applications, such as antibacterial, antifungal, and plant growth-promoting properties. Peptaibols are linear peptides produced by such fungi, with more than 440 compounds described to date, including tricholongins, longibrachins, trichobrachins, and trichovirins. Peptaibols are synthesized by non-ribosomal peptide synthetases and they have several biological activities. Our research group isolated four peptaibols (6DP2, 6DP3, 6DP4, and 6DP5) with antifungal activity against the plant pathogen Colletotrichum gloeosporioides and the proteasome (a cancer chemotherapy target) from Trichoderma sp. P8BDA1F1, an endophytic fungus from Begonia venosa. The ethyl acetate extract of this endophyte showed activity of 6.01% and 75% against C. gloeosporioides and the proteasome, respectively. The isolated compounds were identified by MS/MS and compared to literature data, suggesting the presence of trilongins BI, BII, BIII, and BIV, which are peptaibols containing 20 amino acid residues. The minimum inhibitory concentration against C. gloeosporioides was 40 μM for trilongin BI, 320 μM for trilongin BII, 160 μM for trilongin BIII, and 310 μM for trilongin BIV. BI–BIV trilongins inhibited proteasome ChTL activity, with IC50 values of 6.5 ± 2.7; 4.7 ± 1.8; 6.3 ± 2.2; and 2.7 ± 0.5 μM, respectively. The compounds were tested ex vivo against the intracellular amastigotes of Leishmania (L.) infantum but showed no selectivity. It is the first report of trilongins BI–BIV with antifungal activity against C. gloeosporioides and the proteasome target. |
id |
UNSP_efdc07dc0dd84b810e78d82e6669d299 |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/200359 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Antifungal compounds with anticancer potential from Trichoderma sp. P8BDA1F1, an endophytic fungus from Begonia venosaAlcatrazes IslandColletotrichum gloeosporioidesPeptaibioticsPeptaibolsProteasomeFungi in the genus Trichoderma are notorious producers of secondary metabolites with diverse applications, such as antibacterial, antifungal, and plant growth-promoting properties. Peptaibols are linear peptides produced by such fungi, with more than 440 compounds described to date, including tricholongins, longibrachins, trichobrachins, and trichovirins. Peptaibols are synthesized by non-ribosomal peptide synthetases and they have several biological activities. Our research group isolated four peptaibols (6DP2, 6DP3, 6DP4, and 6DP5) with antifungal activity against the plant pathogen Colletotrichum gloeosporioides and the proteasome (a cancer chemotherapy target) from Trichoderma sp. P8BDA1F1, an endophytic fungus from Begonia venosa. The ethyl acetate extract of this endophyte showed activity of 6.01% and 75% against C. gloeosporioides and the proteasome, respectively. The isolated compounds were identified by MS/MS and compared to literature data, suggesting the presence of trilongins BI, BII, BIII, and BIV, which are peptaibols containing 20 amino acid residues. The minimum inhibitory concentration against C. gloeosporioides was 40 μM for trilongin BI, 320 μM for trilongin BII, 160 μM for trilongin BIII, and 310 μM for trilongin BIV. BI–BIV trilongins inhibited proteasome ChTL activity, with IC50 values of 6.5 ± 2.7; 4.7 ± 1.8; 6.3 ± 2.2; and 2.7 ± 0.5 μM, respectively. The compounds were tested ex vivo against the intracellular amastigotes of Leishmania (L.) infantum but showed no selectivity. It is the first report of trilongins BI–BIV with antifungal activity against C. gloeosporioides and the proteasome target.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Departamento de Ciências Exatas Universidade de São Paulo Escola Superior de Agricultura “Luiz de Queiroz”, CP 9Centro Nacional de Pesquisa em Energia e Materiais Laboratório Nacional de Biociências, CP 6192Instituto Adolfo Lutz Centro de Parasitologia e MicologiaDepartamento de Biologia Geral e Aplicada Universidade Estadual Paulista (UNESP)Centro de Estudos de Insetos Sociais Universidade Estadual Paulista (UNESP)Departamento de Biologia Geral e Aplicada Universidade Estadual Paulista (UNESP)Centro de Estudos de Insetos Sociais Universidade Estadual Paulista (UNESP)CNPq: 142079/2016-2FAPESP: 2013/50228-8FAPESP: 2014/10753-9FAPESP: 2014/12021-5FAPESP: 2014/15760-3CAPES: 88881.133610/2016-01Universidade de São Paulo (USP)Laboratório Nacional de BiociênciasCentro de Parasitologia e MicologiaUniversidade Estadual Paulista (Unesp)Grigoletto, Diana F.Trivella, Daniela B. B.Tempone, André G.Rodrigues, André [UNESP]Correia, Ana Maria L. [UNESP]Lira, Simone P.2020-12-12T02:04:34Z2020-12-12T02:04:34Z2020-09-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article989-997http://dx.doi.org/10.1007/s42770-020-00270-9Brazilian Journal of Microbiology, v. 51, n. 3, p. 989-997, 2020.1678-44051517-8382http://hdl.handle.net/11449/20035910.1007/s42770-020-00270-92-s2.0-85084141981Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBrazilian Journal of Microbiologyinfo:eu-repo/semantics/openAccess2024-04-11T14:57:20Zoai:repositorio.unesp.br:11449/200359Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T19:32:51.712992Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Antifungal compounds with anticancer potential from Trichoderma sp. P8BDA1F1, an endophytic fungus from Begonia venosa |
title |
Antifungal compounds with anticancer potential from Trichoderma sp. P8BDA1F1, an endophytic fungus from Begonia venosa |
spellingShingle |
Antifungal compounds with anticancer potential from Trichoderma sp. P8BDA1F1, an endophytic fungus from Begonia venosa Grigoletto, Diana F. Alcatrazes Island Colletotrichum gloeosporioides Peptaibiotics Peptaibols Proteasome |
title_short |
Antifungal compounds with anticancer potential from Trichoderma sp. P8BDA1F1, an endophytic fungus from Begonia venosa |
title_full |
Antifungal compounds with anticancer potential from Trichoderma sp. P8BDA1F1, an endophytic fungus from Begonia venosa |
title_fullStr |
Antifungal compounds with anticancer potential from Trichoderma sp. P8BDA1F1, an endophytic fungus from Begonia venosa |
title_full_unstemmed |
Antifungal compounds with anticancer potential from Trichoderma sp. P8BDA1F1, an endophytic fungus from Begonia venosa |
title_sort |
Antifungal compounds with anticancer potential from Trichoderma sp. P8BDA1F1, an endophytic fungus from Begonia venosa |
author |
Grigoletto, Diana F. |
author_facet |
Grigoletto, Diana F. Trivella, Daniela B. B. Tempone, André G. Rodrigues, André [UNESP] Correia, Ana Maria L. [UNESP] Lira, Simone P. |
author_role |
author |
author2 |
Trivella, Daniela B. B. Tempone, André G. Rodrigues, André [UNESP] Correia, Ana Maria L. [UNESP] Lira, Simone P. |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) Laboratório Nacional de Biociências Centro de Parasitologia e Micologia Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Grigoletto, Diana F. Trivella, Daniela B. B. Tempone, André G. Rodrigues, André [UNESP] Correia, Ana Maria L. [UNESP] Lira, Simone P. |
dc.subject.por.fl_str_mv |
Alcatrazes Island Colletotrichum gloeosporioides Peptaibiotics Peptaibols Proteasome |
topic |
Alcatrazes Island Colletotrichum gloeosporioides Peptaibiotics Peptaibols Proteasome |
description |
Fungi in the genus Trichoderma are notorious producers of secondary metabolites with diverse applications, such as antibacterial, antifungal, and plant growth-promoting properties. Peptaibols are linear peptides produced by such fungi, with more than 440 compounds described to date, including tricholongins, longibrachins, trichobrachins, and trichovirins. Peptaibols are synthesized by non-ribosomal peptide synthetases and they have several biological activities. Our research group isolated four peptaibols (6DP2, 6DP3, 6DP4, and 6DP5) with antifungal activity against the plant pathogen Colletotrichum gloeosporioides and the proteasome (a cancer chemotherapy target) from Trichoderma sp. P8BDA1F1, an endophytic fungus from Begonia venosa. The ethyl acetate extract of this endophyte showed activity of 6.01% and 75% against C. gloeosporioides and the proteasome, respectively. The isolated compounds were identified by MS/MS and compared to literature data, suggesting the presence of trilongins BI, BII, BIII, and BIV, which are peptaibols containing 20 amino acid residues. The minimum inhibitory concentration against C. gloeosporioides was 40 μM for trilongin BI, 320 μM for trilongin BII, 160 μM for trilongin BIII, and 310 μM for trilongin BIV. BI–BIV trilongins inhibited proteasome ChTL activity, with IC50 values of 6.5 ± 2.7; 4.7 ± 1.8; 6.3 ± 2.2; and 2.7 ± 0.5 μM, respectively. The compounds were tested ex vivo against the intracellular amastigotes of Leishmania (L.) infantum but showed no selectivity. It is the first report of trilongins BI–BIV with antifungal activity against C. gloeosporioides and the proteasome target. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-12T02:04:34Z 2020-12-12T02:04:34Z 2020-09-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s42770-020-00270-9 Brazilian Journal of Microbiology, v. 51, n. 3, p. 989-997, 2020. 1678-4405 1517-8382 http://hdl.handle.net/11449/200359 10.1007/s42770-020-00270-9 2-s2.0-85084141981 |
url |
http://dx.doi.org/10.1007/s42770-020-00270-9 http://hdl.handle.net/11449/200359 |
identifier_str_mv |
Brazilian Journal of Microbiology, v. 51, n. 3, p. 989-997, 2020. 1678-4405 1517-8382 10.1007/s42770-020-00270-9 2-s2.0-85084141981 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Brazilian Journal of Microbiology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
989-997 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129083370897408 |