Transcriptome Responses of Wild Arachis to UV-C Exposure Reveal Genes Involved in General Plant Defense and Priming
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3390/plants11030408 http://hdl.handle.net/11449/223367 |
Resumo: | Stress priming is an important strategy for enhancing plant defense capacity to deal with environmental challenges and involves reprogrammed transcriptional responses. Although ultraviolet (UV) light exposure is a widely adopted approach to elicit stress memory and tolerance in plants, the molecular mechanisms underlying UV-mediated plant priming tolerance are not fully understood. Here, we investigated the changes in the global transcriptome profile of wild Arachis stenosperma leaves in response to UV-C exposure. A total of 5751 differentially expressed genes (DEGs) were identified, with the majority associated with cell signaling, protein dynamics, hormonal and transcriptional regulation, and secondary metabolic pathways. The expression profiles of DEGs known as indicators of priming state, such as transcription factors, transcriptional regulators and protein kinases, were further characterized. A meta-analysis, followed by qRT-PCR validation, identified 18 metaDEGs as being commonly regulated in response to UV and other primary stresses. These genes are involved in secondary metabolism, basal immunity, cell wall structure and integrity, and may constitute important players in the general defense processes and establishment of a priming state in A. stenosperma. Our findings contribute to a better understanding of transcriptional dynamics involved in wild Arachis adaptation to stressful conditions of their natural habitats. |
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Transcriptome Responses of Wild Arachis to UV-C Exposure Reveal Genes Involved in General Plant Defense and PrimingAbiotic stressBiotic stressCercosporidium personatumDroughtMeloidogyne arenariaMeta-analysisUltraviolet lightStress priming is an important strategy for enhancing plant defense capacity to deal with environmental challenges and involves reprogrammed transcriptional responses. Although ultraviolet (UV) light exposure is a widely adopted approach to elicit stress memory and tolerance in plants, the molecular mechanisms underlying UV-mediated plant priming tolerance are not fully understood. Here, we investigated the changes in the global transcriptome profile of wild Arachis stenosperma leaves in response to UV-C exposure. A total of 5751 differentially expressed genes (DEGs) were identified, with the majority associated with cell signaling, protein dynamics, hormonal and transcriptional regulation, and secondary metabolic pathways. The expression profiles of DEGs known as indicators of priming state, such as transcription factors, transcriptional regulators and protein kinases, were further characterized. A meta-analysis, followed by qRT-PCR validation, identified 18 metaDEGs as being commonly regulated in response to UV and other primary stresses. These genes are involved in secondary metabolism, basal immunity, cell wall structure and integrity, and may constitute important players in the general defense processes and establishment of a priming state in A. stenosperma. Our findings contribute to a better understanding of transcriptional dynamics involved in wild Arachis adaptation to stressful conditions of their natural habitats.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Apoio à Pesquisa do Distrito FederalEMBRAPA Recursos Genéticos e Biotecnologia, DFInstituto Nacional de Ciência e Tecnologia-INCT PlantStress Biotech-Embrapa, DFCIRAD UMR AGAPInstituto de Biociências Department de Genética Universidade Estadual Paulista (UNESP), SPInstituto de Biociências Department de Genética Universidade Estadual Paulista (UNESP), SPFundação de Apoio à Pesquisa do Distrito Federal: 0193.001565/2017Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)UMR AGAPUniversidade Estadual Paulista (UNESP)Martins, Andressa Cunha QuintanaMota, Ana Paula ZottaCarvalho, Paula Andrea Sampaio Vasconcelos [UNESP]Passos, Mario Alfredo SaraivaGimenes, Marcos AparecidoGuimaraes, Patricia MessenbergBrasileiro, Ana Cristina Miranda2022-04-28T19:50:13Z2022-04-28T19:50:13Z2022-02-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.3390/plants11030408Plants, v. 11, n. 3, 2022.2223-7747http://hdl.handle.net/11449/22336710.3390/plants110304082-s2.0-85123772700Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPlantsinfo:eu-repo/semantics/openAccess2022-04-28T19:50:13Zoai:repositorio.unesp.br:11449/223367Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T14:26:09.978844Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Transcriptome Responses of Wild Arachis to UV-C Exposure Reveal Genes Involved in General Plant Defense and Priming |
title |
Transcriptome Responses of Wild Arachis to UV-C Exposure Reveal Genes Involved in General Plant Defense and Priming |
spellingShingle |
Transcriptome Responses of Wild Arachis to UV-C Exposure Reveal Genes Involved in General Plant Defense and Priming Martins, Andressa Cunha Quintana Abiotic stress Biotic stress Cercosporidium personatum Drought Meloidogyne arenaria Meta-analysis Ultraviolet light |
title_short |
Transcriptome Responses of Wild Arachis to UV-C Exposure Reveal Genes Involved in General Plant Defense and Priming |
title_full |
Transcriptome Responses of Wild Arachis to UV-C Exposure Reveal Genes Involved in General Plant Defense and Priming |
title_fullStr |
Transcriptome Responses of Wild Arachis to UV-C Exposure Reveal Genes Involved in General Plant Defense and Priming |
title_full_unstemmed |
Transcriptome Responses of Wild Arachis to UV-C Exposure Reveal Genes Involved in General Plant Defense and Priming |
title_sort |
Transcriptome Responses of Wild Arachis to UV-C Exposure Reveal Genes Involved in General Plant Defense and Priming |
author |
Martins, Andressa Cunha Quintana |
author_facet |
Martins, Andressa Cunha Quintana Mota, Ana Paula Zotta Carvalho, Paula Andrea Sampaio Vasconcelos [UNESP] Passos, Mario Alfredo Saraiva Gimenes, Marcos Aparecido Guimaraes, Patricia Messenberg Brasileiro, Ana Cristina Miranda |
author_role |
author |
author2 |
Mota, Ana Paula Zotta Carvalho, Paula Andrea Sampaio Vasconcelos [UNESP] Passos, Mario Alfredo Saraiva Gimenes, Marcos Aparecido Guimaraes, Patricia Messenberg Brasileiro, Ana Cristina Miranda |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA) UMR AGAP Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
Martins, Andressa Cunha Quintana Mota, Ana Paula Zotta Carvalho, Paula Andrea Sampaio Vasconcelos [UNESP] Passos, Mario Alfredo Saraiva Gimenes, Marcos Aparecido Guimaraes, Patricia Messenberg Brasileiro, Ana Cristina Miranda |
dc.subject.por.fl_str_mv |
Abiotic stress Biotic stress Cercosporidium personatum Drought Meloidogyne arenaria Meta-analysis Ultraviolet light |
topic |
Abiotic stress Biotic stress Cercosporidium personatum Drought Meloidogyne arenaria Meta-analysis Ultraviolet light |
description |
Stress priming is an important strategy for enhancing plant defense capacity to deal with environmental challenges and involves reprogrammed transcriptional responses. Although ultraviolet (UV) light exposure is a widely adopted approach to elicit stress memory and tolerance in plants, the molecular mechanisms underlying UV-mediated plant priming tolerance are not fully understood. Here, we investigated the changes in the global transcriptome profile of wild Arachis stenosperma leaves in response to UV-C exposure. A total of 5751 differentially expressed genes (DEGs) were identified, with the majority associated with cell signaling, protein dynamics, hormonal and transcriptional regulation, and secondary metabolic pathways. The expression profiles of DEGs known as indicators of priming state, such as transcription factors, transcriptional regulators and protein kinases, were further characterized. A meta-analysis, followed by qRT-PCR validation, identified 18 metaDEGs as being commonly regulated in response to UV and other primary stresses. These genes are involved in secondary metabolism, basal immunity, cell wall structure and integrity, and may constitute important players in the general defense processes and establishment of a priming state in A. stenosperma. Our findings contribute to a better understanding of transcriptional dynamics involved in wild Arachis adaptation to stressful conditions of their natural habitats. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-04-28T19:50:13Z 2022-04-28T19:50:13Z 2022-02-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3390/plants11030408 Plants, v. 11, n. 3, 2022. 2223-7747 http://hdl.handle.net/11449/223367 10.3390/plants11030408 2-s2.0-85123772700 |
url |
http://dx.doi.org/10.3390/plants11030408 http://hdl.handle.net/11449/223367 |
identifier_str_mv |
Plants, v. 11, n. 3, 2022. 2223-7747 10.3390/plants11030408 2-s2.0-85123772700 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Plants |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1808128360239333376 |