Detection and localization of small nuclear ribonucleoproteins (snRNPs) in trypanosomatids using anti-m3G antibodies

Detalhes bibliográficos
Autor(a) principal: Bosetto, Maira Cegatti [UNESP]
Data de Publicação: 2008
Outros Autores: Mortara, Renato Arruda, Ambrósio, Daniela Luz [UNESP], Passerini, Gabriela Duó [UNESP], da Silva, Marco Túlio Alves [UNESP], Okuda, Erica Sayuri [UNESP], Cicarelli, Regina Maria Barretto [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://hdl.handle.net/11449/225162
Resumo: This work reports for the first time the identification and immunolocalization, by confocal and conventional indirect immunofluorescence, of m3G epitopes present in ribonucleoproteins of the following trypanosomatids: Trypanosoma cruzi epimastigotes of three different strains, Blastocrithidia ssp., and Leishmania major promastigotes. The identity of these epitopes and hence the spe~cificity of the anti-m3G monoclonal antibody were ascertained through competition reaction with 7-methylguanosine that blocks the Ig binding sites, abolishing the fluorescence in all the parasites tested and showing a specific perinuclear localization of the snRNPs, which suggests their nuclear reimport in the parasites. Using an immunoprecipitation technique, it was also possible. to confirm the presence of the trimethylguanosine epitopes in trypanosomatids.
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spelling Detection and localization of small nuclear ribonucleoproteins (snRNPs) in trypanosomatids using anti-m3G antibodiesThis work reports for the first time the identification and immunolocalization, by confocal and conventional indirect immunofluorescence, of m3G epitopes present in ribonucleoproteins of the following trypanosomatids: Trypanosoma cruzi epimastigotes of three different strains, Blastocrithidia ssp., and Leishmania major promastigotes. The identity of these epitopes and hence the spe~cificity of the anti-m3G monoclonal antibody were ascertained through competition reaction with 7-methylguanosine that blocks the Ig binding sites, abolishing the fluorescence in all the parasites tested and showing a specific perinuclear localization of the snRNPs, which suggests their nuclear reimport in the parasites. Using an immunoprecipitation technique, it was also possible. to confirm the presence of the trimethylguanosine epitopes in trypanosomatids.Departamento de Ciências Biológicas Faculdade de Ciências Farmacêuticas Universidade Estadual Paulista, Rodovia Araraquara-Jaú, km 01, 14801-902 Araraquara, São PauloDepartamento de Microbiologia Imunologia e Parasitologia Escola Paulista de Medicina Universidade Federal de São Paulo, São PauloDepartamento de Ciências Biológicas Faculdade de Ciências Farmacêuticas Universidade Estadual Paulista, Rodovia Araraquara-Jaú, km 01, 14801-902 Araraquara, São PauloUniversidade Estadual Paulista (UNESP)Universidade Federal de São Paulo (UNIFESP)Bosetto, Maira Cegatti [UNESP]Mortara, Renato ArrudaAmbrósio, Daniela Luz [UNESP]Passerini, Gabriela Duó [UNESP]da Silva, Marco Túlio Alves [UNESP]Okuda, Erica Sayuri [UNESP]Cicarelli, Regina Maria Barretto [UNESP]2022-04-28T20:40:40Z2022-04-28T20:40:40Z2008-05-19info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article95-99Japanese Journal of Infectious Diseases, v. 61, n. 2, p. 95-99, 2008.1344-6304http://hdl.handle.net/11449/2251622-s2.0-43449128414Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJapanese Journal of Infectious Diseasesinfo:eu-repo/semantics/openAccess2024-06-24T13:08:14Zoai:repositorio.unesp.br:11449/225162Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T21:56:10.110469Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Detection and localization of small nuclear ribonucleoproteins (snRNPs) in trypanosomatids using anti-m3G antibodies
title Detection and localization of small nuclear ribonucleoproteins (snRNPs) in trypanosomatids using anti-m3G antibodies
spellingShingle Detection and localization of small nuclear ribonucleoproteins (snRNPs) in trypanosomatids using anti-m3G antibodies
Bosetto, Maira Cegatti [UNESP]
title_short Detection and localization of small nuclear ribonucleoproteins (snRNPs) in trypanosomatids using anti-m3G antibodies
title_full Detection and localization of small nuclear ribonucleoproteins (snRNPs) in trypanosomatids using anti-m3G antibodies
title_fullStr Detection and localization of small nuclear ribonucleoproteins (snRNPs) in trypanosomatids using anti-m3G antibodies
title_full_unstemmed Detection and localization of small nuclear ribonucleoproteins (snRNPs) in trypanosomatids using anti-m3G antibodies
title_sort Detection and localization of small nuclear ribonucleoproteins (snRNPs) in trypanosomatids using anti-m3G antibodies
author Bosetto, Maira Cegatti [UNESP]
author_facet Bosetto, Maira Cegatti [UNESP]
Mortara, Renato Arruda
Ambrósio, Daniela Luz [UNESP]
Passerini, Gabriela Duó [UNESP]
da Silva, Marco Túlio Alves [UNESP]
Okuda, Erica Sayuri [UNESP]
Cicarelli, Regina Maria Barretto [UNESP]
author_role author
author2 Mortara, Renato Arruda
Ambrósio, Daniela Luz [UNESP]
Passerini, Gabriela Duó [UNESP]
da Silva, Marco Túlio Alves [UNESP]
Okuda, Erica Sayuri [UNESP]
Cicarelli, Regina Maria Barretto [UNESP]
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (UNESP)
Universidade Federal de São Paulo (UNIFESP)
dc.contributor.author.fl_str_mv Bosetto, Maira Cegatti [UNESP]
Mortara, Renato Arruda
Ambrósio, Daniela Luz [UNESP]
Passerini, Gabriela Duó [UNESP]
da Silva, Marco Túlio Alves [UNESP]
Okuda, Erica Sayuri [UNESP]
Cicarelli, Regina Maria Barretto [UNESP]
description This work reports for the first time the identification and immunolocalization, by confocal and conventional indirect immunofluorescence, of m3G epitopes present in ribonucleoproteins of the following trypanosomatids: Trypanosoma cruzi epimastigotes of three different strains, Blastocrithidia ssp., and Leishmania major promastigotes. The identity of these epitopes and hence the spe~cificity of the anti-m3G monoclonal antibody were ascertained through competition reaction with 7-methylguanosine that blocks the Ig binding sites, abolishing the fluorescence in all the parasites tested and showing a specific perinuclear localization of the snRNPs, which suggests their nuclear reimport in the parasites. Using an immunoprecipitation technique, it was also possible. to confirm the presence of the trimethylguanosine epitopes in trypanosomatids.
publishDate 2008
dc.date.none.fl_str_mv 2008-05-19
2022-04-28T20:40:40Z
2022-04-28T20:40:40Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv Japanese Journal of Infectious Diseases, v. 61, n. 2, p. 95-99, 2008.
1344-6304
http://hdl.handle.net/11449/225162
2-s2.0-43449128414
identifier_str_mv Japanese Journal of Infectious Diseases, v. 61, n. 2, p. 95-99, 2008.
1344-6304
2-s2.0-43449128414
url http://hdl.handle.net/11449/225162
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Japanese Journal of Infectious Diseases
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 95-99
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1808129375146606592