Fibrin biopolymer incorporated with antimicrobial agents: a proposal for coating denture bases
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3390/ma14071618 http://hdl.handle.net/11449/207542 |
Resumo: | The characteristics of the denture base surface, in combination with the oral environment, promote the colonization and development of Candida albicans biofilm, which is the main cause of denture stomatitis. This study evaluated the effectiveness of fibrin biopolymer with digluconate chlorhexidine or Punica granatum alcoholic extract to prevent C. albicans biofilm. Conventional heat polymerized and pre-polymerized poly(methyl methacrylate) (PMMA) circular specimens (102 mm) were fabricated (n = 504) and randomly divided into groups: no treatment (control-CT), fibrin biopolymer coating (FB), fibrin biopolymer with P. granatum (FBPg), or digluconate of chlorhexidine (FBCh) coating. The specimens were inoculated with C. albicans SC5314 (1*107 cells/mL) and incubated for 24, 48, and 72 h. Crystal violet and colony-forming unit assays were used to quantify the total biofilm biomass and biofilm-living cells. A qualitative analysis was performed using confocal laser scanning microscopy. Data obtained are expressed as means and standard deviations and were statistically analyzed using a three-way analysis of variance (p = 0.05). The FBPg and FBCh groups inhibited the growth of C. albicans biofilm in both PMMA materials analyzed, with FBCh performing better in all periods evaluated (p < 0.0001). The colony forming unit (CFU) assay showed that the FB group favored the C. albicans biofilm growth at 24 h and 48 h (p < 0.0001), with no differences with CT group at 72 h (p = 0.790). All groups showed an enhancement in biofilm development up to 72 h (p < 0.0001), except the FBCh group (p = 0.100). No statistical differences were found between the PMMA base materials (p > 0.050), except in the FB group (p < 0.0001). Fibrin biopolymer, albeit a scaffold for the growth of C. albicans, when combined with chlorhexidine digluconate or P. granatum, demonstrated excellent performance as a drug delivery system, preventing and controlling the formation of denture biofilm. |
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Fibrin biopolymer incorporated with antimicrobial agents: a proposal for coating denture basesAcrylic resinCAD-CAMCandida albicansComplete dentureDenture stomatitisThe characteristics of the denture base surface, in combination with the oral environment, promote the colonization and development of Candida albicans biofilm, which is the main cause of denture stomatitis. This study evaluated the effectiveness of fibrin biopolymer with digluconate chlorhexidine or Punica granatum alcoholic extract to prevent C. albicans biofilm. Conventional heat polymerized and pre-polymerized poly(methyl methacrylate) (PMMA) circular specimens (102 mm) were fabricated (n = 504) and randomly divided into groups: no treatment (control-CT), fibrin biopolymer coating (FB), fibrin biopolymer with P. granatum (FBPg), or digluconate of chlorhexidine (FBCh) coating. The specimens were inoculated with C. albicans SC5314 (1*107 cells/mL) and incubated for 24, 48, and 72 h. Crystal violet and colony-forming unit assays were used to quantify the total biofilm biomass and biofilm-living cells. A qualitative analysis was performed using confocal laser scanning microscopy. Data obtained are expressed as means and standard deviations and were statistically analyzed using a three-way analysis of variance (p = 0.05). The FBPg and FBCh groups inhibited the growth of C. albicans biofilm in both PMMA materials analyzed, with FBCh performing better in all periods evaluated (p < 0.0001). The colony forming unit (CFU) assay showed that the FB group favored the C. albicans biofilm growth at 24 h and 48 h (p < 0.0001), with no differences with CT group at 72 h (p = 0.790). All groups showed an enhancement in biofilm development up to 72 h (p < 0.0001), except the FBCh group (p = 0.100). No statistical differences were found between the PMMA base materials (p > 0.050), except in the FB group (p < 0.0001). Fibrin biopolymer, albeit a scaffold for the growth of C. albicans, when combined with chlorhexidine digluconate or P. granatum, demonstrated excellent performance as a drug delivery system, preventing and controlling the formation of denture biofilm.Department of Periodontics and Prosthodontics Dental School of Bauru FOB-USP-University of São Paulo, Al. Octávio Pinheiro Brisola, 9-75School of Dentistry Universidad Espíritu SantDepartment of Surgery Stomatology Pathology and Radiology Dental School of Bauru FOB-USP-University of São Paulo, Al. Octávio Pinheiro Brisola, 9-75Department of Infectology Dermatology Imaging Diagnosis and Radiotherapy Botucatu Medical School FMB São Paulo State University UNESP, Av. Prof. Mário Rubens Guimarães MontenegroCenter for the Studies of Venoms and Venomous Animals CEVAP São Paulo State University UNESP, Av. Universitária, 3780Department de Pediatric Dentistry Orthodontics and Public Health Dental School of Bauru FOB-USP-University of São Paulo, Al. Octávio Pinheiro Brisola, 9-75Department of Infectology Dermatology Imaging Diagnosis and Radiotherapy Botucatu Medical School FMB São Paulo State University UNESP, Av. Prof. Mário Rubens Guimarães MontenegroCenter for the Studies of Venoms and Venomous Animals CEVAP São Paulo State University UNESP, Av. Universitária, 3780Universidade de São Paulo (USP)Universidad Espíritu SantUniversidade Estadual Paulista (Unesp)Venante, Helena SandriniChappuis-Chocano, Ana PaulaMarcillo-Toala, Oscar OswaldoDa Silva, Rafaela AlvesDa Costa, Rodrigo Moreira BringelPordeus, Mariana DominguesBarraviera, Benedito [UNESP]Junior, Rui Seabra Ferreira [UNESP]Lara, Vanessa SoaresNeppelenbroek, Karin HermanaHonório, Heitor MarquesPorto, Vinicius Carvalho2021-06-25T10:56:57Z2021-06-25T10:56:57Z2021-04-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.3390/ma14071618Materials, v. 14, n. 7, 2021.1996-1944http://hdl.handle.net/11449/20754210.3390/ma140716182-s2.0-85103402167Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMaterialsinfo:eu-repo/semantics/openAccess2021-10-23T17:30:43Zoai:repositorio.unesp.br:11449/207542Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T17:30:43Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Fibrin biopolymer incorporated with antimicrobial agents: a proposal for coating denture bases |
title |
Fibrin biopolymer incorporated with antimicrobial agents: a proposal for coating denture bases |
spellingShingle |
Fibrin biopolymer incorporated with antimicrobial agents: a proposal for coating denture bases Venante, Helena Sandrini Acrylic resin CAD-CAM Candida albicans Complete denture Denture stomatitis |
title_short |
Fibrin biopolymer incorporated with antimicrobial agents: a proposal for coating denture bases |
title_full |
Fibrin biopolymer incorporated with antimicrobial agents: a proposal for coating denture bases |
title_fullStr |
Fibrin biopolymer incorporated with antimicrobial agents: a proposal for coating denture bases |
title_full_unstemmed |
Fibrin biopolymer incorporated with antimicrobial agents: a proposal for coating denture bases |
title_sort |
Fibrin biopolymer incorporated with antimicrobial agents: a proposal for coating denture bases |
author |
Venante, Helena Sandrini |
author_facet |
Venante, Helena Sandrini Chappuis-Chocano, Ana Paula Marcillo-Toala, Oscar Oswaldo Da Silva, Rafaela Alves Da Costa, Rodrigo Moreira Bringel Pordeus, Mariana Domingues Barraviera, Benedito [UNESP] Junior, Rui Seabra Ferreira [UNESP] Lara, Vanessa Soares Neppelenbroek, Karin Hermana Honório, Heitor Marques Porto, Vinicius Carvalho |
author_role |
author |
author2 |
Chappuis-Chocano, Ana Paula Marcillo-Toala, Oscar Oswaldo Da Silva, Rafaela Alves Da Costa, Rodrigo Moreira Bringel Pordeus, Mariana Domingues Barraviera, Benedito [UNESP] Junior, Rui Seabra Ferreira [UNESP] Lara, Vanessa Soares Neppelenbroek, Karin Hermana Honório, Heitor Marques Porto, Vinicius Carvalho |
author2_role |
author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) Universidad Espíritu Sant Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Venante, Helena Sandrini Chappuis-Chocano, Ana Paula Marcillo-Toala, Oscar Oswaldo Da Silva, Rafaela Alves Da Costa, Rodrigo Moreira Bringel Pordeus, Mariana Domingues Barraviera, Benedito [UNESP] Junior, Rui Seabra Ferreira [UNESP] Lara, Vanessa Soares Neppelenbroek, Karin Hermana Honório, Heitor Marques Porto, Vinicius Carvalho |
dc.subject.por.fl_str_mv |
Acrylic resin CAD-CAM Candida albicans Complete denture Denture stomatitis |
topic |
Acrylic resin CAD-CAM Candida albicans Complete denture Denture stomatitis |
description |
The characteristics of the denture base surface, in combination with the oral environment, promote the colonization and development of Candida albicans biofilm, which is the main cause of denture stomatitis. This study evaluated the effectiveness of fibrin biopolymer with digluconate chlorhexidine or Punica granatum alcoholic extract to prevent C. albicans biofilm. Conventional heat polymerized and pre-polymerized poly(methyl methacrylate) (PMMA) circular specimens (102 mm) were fabricated (n = 504) and randomly divided into groups: no treatment (control-CT), fibrin biopolymer coating (FB), fibrin biopolymer with P. granatum (FBPg), or digluconate of chlorhexidine (FBCh) coating. The specimens were inoculated with C. albicans SC5314 (1*107 cells/mL) and incubated for 24, 48, and 72 h. Crystal violet and colony-forming unit assays were used to quantify the total biofilm biomass and biofilm-living cells. A qualitative analysis was performed using confocal laser scanning microscopy. Data obtained are expressed as means and standard deviations and were statistically analyzed using a three-way analysis of variance (p = 0.05). The FBPg and FBCh groups inhibited the growth of C. albicans biofilm in both PMMA materials analyzed, with FBCh performing better in all periods evaluated (p < 0.0001). The colony forming unit (CFU) assay showed that the FB group favored the C. albicans biofilm growth at 24 h and 48 h (p < 0.0001), with no differences with CT group at 72 h (p = 0.790). All groups showed an enhancement in biofilm development up to 72 h (p < 0.0001), except the FBCh group (p = 0.100). No statistical differences were found between the PMMA base materials (p > 0.050), except in the FB group (p < 0.0001). Fibrin biopolymer, albeit a scaffold for the growth of C. albicans, when combined with chlorhexidine digluconate or P. granatum, demonstrated excellent performance as a drug delivery system, preventing and controlling the formation of denture biofilm. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-06-25T10:56:57Z 2021-06-25T10:56:57Z 2021-04-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3390/ma14071618 Materials, v. 14, n. 7, 2021. 1996-1944 http://hdl.handle.net/11449/207542 10.3390/ma14071618 2-s2.0-85103402167 |
url |
http://dx.doi.org/10.3390/ma14071618 http://hdl.handle.net/11449/207542 |
identifier_str_mv |
Materials, v. 14, n. 7, 2021. 1996-1944 10.3390/ma14071618 2-s2.0-85103402167 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Materials |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1799965380766072832 |