Identification of Eimeria mitis and Eimeria praecox in broiler feces using polymerase chain reaction
Autor(a) principal: | |
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Data de Publicação: | 2004 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/S1516-635X2004000400010 http://hdl.handle.net/11449/26418 |
Resumo: | There are few reports concerning the epidemiology of Eimeria praecox and Eimeria mitis in Brazil. In the present experiment, the polymerase chain reaction (PCR) was used to identify these species in 156 samples of broiler chicken feces from several Brazilian states and the Federal District. Oocysts present in feces samples were purified by sodium chloride flotation followed by addition of DNAzol reagent (Invitrogen®) for extraction of genomic DNA. DNA was precipitated and stored following DNAzol reagent manufacture's instructions. The primers and PCR conditions were as described by Schnitzler et al. (1999). In the 156 field samples analyzed by PCR, 70 and 45 were positive for E. praecox and E. mitis, respectively. In this study we have shown that DNA extraction using DNAzol followed by PCR can be a useful tool in epidemiological studies, since it provides fast and reliable detection of Eimeria sp. in field samples. |
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Repositório Institucional da UNESP |
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Identification of Eimeria mitis and Eimeria praecox in broiler feces using polymerase chain reactiondiagnosisEimeria mitisEimeria praecoxepidemiologypolymerase chain reactionThere are few reports concerning the epidemiology of Eimeria praecox and Eimeria mitis in Brazil. In the present experiment, the polymerase chain reaction (PCR) was used to identify these species in 156 samples of broiler chicken feces from several Brazilian states and the Federal District. Oocysts present in feces samples were purified by sodium chloride flotation followed by addition of DNAzol reagent (Invitrogen®) for extraction of genomic DNA. DNA was precipitated and stored following DNAzol reagent manufacture's instructions. The primers and PCR conditions were as described by Schnitzler et al. (1999). In the 156 field samples analyzed by PCR, 70 and 45 were positive for E. praecox and E. mitis, respectively. In this study we have shown that DNA extraction using DNAzol followed by PCR can be a useful tool in epidemiological studies, since it provides fast and reliable detection of Eimeria sp. in field samples.Universidade Estadual PaulistaUniversidade Estadual PaulistaFundação APINCO de Ciência e Tecnologia AvícolasUniversidade Estadual Paulista (Unesp)Meireles, Marcelo Vasconcelos [UNESP]Roberto, LORiera, RF2014-05-20T15:07:13Z2014-05-20T15:07:13Z2004-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article249-252application/pdfhttp://dx.doi.org/10.1590/S1516-635X2004000400010Revista Brasileira de Ciência Avícola. Fundação APINCO de Ciência e Tecnologia Avícolas, v. 6, n. 4, p. 249-252, 2004.1516-635Xhttp://hdl.handle.net/11449/2641810.1590/S1516-635X2004000400010S1516-635X2004000400010S1516-635X2004000400010.pdf09035138976152740000-0003-0063-5172SciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengRevista Brasileira de Ciência Avícola0.463info:eu-repo/semantics/openAccess2024-09-04T18:03:31Zoai:repositorio.unesp.br:11449/26418Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-04T18:03:31Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Identification of Eimeria mitis and Eimeria praecox in broiler feces using polymerase chain reaction |
title |
Identification of Eimeria mitis and Eimeria praecox in broiler feces using polymerase chain reaction |
spellingShingle |
Identification of Eimeria mitis and Eimeria praecox in broiler feces using polymerase chain reaction Meireles, Marcelo Vasconcelos [UNESP] diagnosis Eimeria mitis Eimeria praecox epidemiology polymerase chain reaction |
title_short |
Identification of Eimeria mitis and Eimeria praecox in broiler feces using polymerase chain reaction |
title_full |
Identification of Eimeria mitis and Eimeria praecox in broiler feces using polymerase chain reaction |
title_fullStr |
Identification of Eimeria mitis and Eimeria praecox in broiler feces using polymerase chain reaction |
title_full_unstemmed |
Identification of Eimeria mitis and Eimeria praecox in broiler feces using polymerase chain reaction |
title_sort |
Identification of Eimeria mitis and Eimeria praecox in broiler feces using polymerase chain reaction |
author |
Meireles, Marcelo Vasconcelos [UNESP] |
author_facet |
Meireles, Marcelo Vasconcelos [UNESP] Roberto, LO Riera, RF |
author_role |
author |
author2 |
Roberto, LO Riera, RF |
author2_role |
author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Meireles, Marcelo Vasconcelos [UNESP] Roberto, LO Riera, RF |
dc.subject.por.fl_str_mv |
diagnosis Eimeria mitis Eimeria praecox epidemiology polymerase chain reaction |
topic |
diagnosis Eimeria mitis Eimeria praecox epidemiology polymerase chain reaction |
description |
There are few reports concerning the epidemiology of Eimeria praecox and Eimeria mitis in Brazil. In the present experiment, the polymerase chain reaction (PCR) was used to identify these species in 156 samples of broiler chicken feces from several Brazilian states and the Federal District. Oocysts present in feces samples were purified by sodium chloride flotation followed by addition of DNAzol reagent (Invitrogen®) for extraction of genomic DNA. DNA was precipitated and stored following DNAzol reagent manufacture's instructions. The primers and PCR conditions were as described by Schnitzler et al. (1999). In the 156 field samples analyzed by PCR, 70 and 45 were positive for E. praecox and E. mitis, respectively. In this study we have shown that DNA extraction using DNAzol followed by PCR can be a useful tool in epidemiological studies, since it provides fast and reliable detection of Eimeria sp. in field samples. |
publishDate |
2004 |
dc.date.none.fl_str_mv |
2004-12-01 2014-05-20T15:07:13Z 2014-05-20T15:07:13Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S1516-635X2004000400010 Revista Brasileira de Ciência Avícola. Fundação APINCO de Ciência e Tecnologia Avícolas, v. 6, n. 4, p. 249-252, 2004. 1516-635X http://hdl.handle.net/11449/26418 10.1590/S1516-635X2004000400010 S1516-635X2004000400010 S1516-635X2004000400010.pdf 0903513897615274 0000-0003-0063-5172 |
url |
http://dx.doi.org/10.1590/S1516-635X2004000400010 http://hdl.handle.net/11449/26418 |
identifier_str_mv |
Revista Brasileira de Ciência Avícola. Fundação APINCO de Ciência e Tecnologia Avícolas, v. 6, n. 4, p. 249-252, 2004. 1516-635X 10.1590/S1516-635X2004000400010 S1516-635X2004000400010 S1516-635X2004000400010.pdf 0903513897615274 0000-0003-0063-5172 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Revista Brasileira de Ciência Avícola 0.463 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
249-252 application/pdf |
dc.publisher.none.fl_str_mv |
Fundação APINCO de Ciência e Tecnologia Avícolas |
publisher.none.fl_str_mv |
Fundação APINCO de Ciência e Tecnologia Avícolas |
dc.source.none.fl_str_mv |
SciELO reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
repositoriounesp@unesp.br |
_version_ |
1826303719746043904 |