Qualidade espermática após seleção por centrifugação em Ovipure® ou gradiente de Percoll® do sêmen ovino criopreservado

• Autor(a) principal: Bergstein, Tacia Gomes [UNESP]
• Data de Publicação: 2013
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://hdl.handle.net/11449/110375
• topic: Ovino - Recursos de germoplasma; Semen - Criopreservação; Reprodução animal; Biotecnologia animal; Semen preservation

Frozen and thawed ovine semen undergo morphofunctional changes that prevent or decrease the efficiency of fertilization. Sperm selection methods have beneficial effects on the selected sperm, improving sperm quality and viability. Four methods of sperm selection were tested using two colloidal silica solutions coated with silane (silica colloid- silane) ou povinilpirrolidone (silica colloid-PVP), varying the volume of colloidal solution. Furthermore, analyses of sperm kinetics in CASA and sperm recovery were carried out. The protocols using silica colloid-silane resulted in greater MT, MP, and %RAP when compared to the methods using silica colloid-PVP and the semen before selection. Silica colloid-PVP had higher sperm recovery when compared to silica colloid-silane. Only the method using 4mL of silica colloid-PVP was not efficient in the selection of best quality samples when compared to the semen before selection. The methods using lower volumes of colloidal solutions did not differ from the methods with higher volumes, being the method with 1mL of silica collois-silane better than the 4mL method. In conclusion the results of the study indicate the superiority of silica colloid-silane in selecting frozen and thawed ovine semen when compared to silica colloid-PVP. The method using 1mL of silica colloid-silane was equally efficient to the standard method and has lower costs, being an interesting alternative to process samples with low sperm concentration