Avaliação de dois diferentes meios de cultura para o desenvolvimento de biopesticidas à base de Bacillus thuringiensis e sua aplicação em larvas de Aedes aegypti
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng por |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.4025/actascitechnol.v35i1.13831 http://hdl.handle.net/11449/74584 |
Resumo: | The bacteria Bacillus thuringiensis var. israelensis (Bti) generates certain toxins with pesticide action, which can be used on the control of transmissible diseases by culicides, specially Aedes aegypti, the dengue vector. This biopesticide has been produced by submerged fermentation and, in Brazil, this production has been made by very little research centers and, more recently, by a unique small enterprise. For the implementation of a viable vectors control program through biopesticides, some studies about culture media are essential in order to join efficiency and low costs. In this way, agroindustrial wastes or by-products have been used as a nutrient source for the culture media production. In this study, corn steep liquor, a corn industrial processing by-product and tryptose, both with/without sugar addition, were compared as culture media. Cellular growth was evaluated by optical density at 620 nm, spore production by total viable cell count and LC50 by bioassays against 4th instar larvae. Among the four examined substrates, the medium composed by glucose plus corn steep liquor presented the best spore production and bioassay results. |
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Avaliação de dois diferentes meios de cultura para o desenvolvimento de biopesticidas à base de Bacillus thuringiensis e sua aplicação em larvas de Aedes aegyptiEvaluation of two different culture media for the development of biopesticides based on Bacillus thuringiensis and their application in larvae of Aedes aegyptiAgroindustrial wasteBiological controlFermentation processThe bacteria Bacillus thuringiensis var. israelensis (Bti) generates certain toxins with pesticide action, which can be used on the control of transmissible diseases by culicides, specially Aedes aegypti, the dengue vector. This biopesticide has been produced by submerged fermentation and, in Brazil, this production has been made by very little research centers and, more recently, by a unique small enterprise. For the implementation of a viable vectors control program through biopesticides, some studies about culture media are essential in order to join efficiency and low costs. In this way, agroindustrial wastes or by-products have been used as a nutrient source for the culture media production. In this study, corn steep liquor, a corn industrial processing by-product and tryptose, both with/without sugar addition, were compared as culture media. Cellular growth was evaluated by optical density at 620 nm, spore production by total viable cell count and LC50 by bioassays against 4th instar larvae. Among the four examined substrates, the medium composed by glucose plus corn steep liquor presented the best spore production and bioassay results.União das Faculdades dos Grandes Lagos, Rua Eduardo Nielsen, 960, 15030-070, Sao Jose do Rio Preto, Sao PauloDepartamento de Engenharia e Tecnologia de Alimentos Universidade Estadual Paulista Julio de Mesquita Filho, São José do Rio Preto, São PauloProbiom Tecnologia P and D Experimental em Ciências Físicas e Naturais Ltda, Campinas, São PauloDepartamento de Engenharia e Tecnologia de Alimentos Universidade Estadual Paulista Julio de Mesquita Filho, São José do Rio Preto, São PauloUnião das Faculdades dos Grandes Lagos (UNILAGO)Universidade Estadual Paulista (Unesp)P&D Experimental em Ciências Físicas e Naturais LtdaErnandes, SamaraBianchi, Vanildo Luiz Del [UNESP]Oliveira Moraes, Iracema de2014-05-27T11:28:24Z2014-05-27T11:28:24Z2013-02-11info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article11-18application/pdfhttp://dx.doi.org/10.4025/actascitechnol.v35i1.13831Acta Scientiarum - Technology, v. 35, n. 1, p. 11-18, 2013.1806-25631807-8664http://hdl.handle.net/11449/7458410.4025/actascitechnol.v35i1.13831WOS:0003153552000032-s2.0-848733876922-s2.0-84873387692.pdf9441894011582350Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengporActa Scientiarum: Technology0.2310,1680,168info:eu-repo/semantics/openAccess2024-01-02T06:24:16Zoai:repositorio.unesp.br:11449/74584Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T21:58:10.187847Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Avaliação de dois diferentes meios de cultura para o desenvolvimento de biopesticidas à base de Bacillus thuringiensis e sua aplicação em larvas de Aedes aegypti Evaluation of two different culture media for the development of biopesticides based on Bacillus thuringiensis and their application in larvae of Aedes aegypti |
title |
Avaliação de dois diferentes meios de cultura para o desenvolvimento de biopesticidas à base de Bacillus thuringiensis e sua aplicação em larvas de Aedes aegypti |
spellingShingle |
Avaliação de dois diferentes meios de cultura para o desenvolvimento de biopesticidas à base de Bacillus thuringiensis e sua aplicação em larvas de Aedes aegypti Ernandes, Samara Agroindustrial waste Biological control Fermentation process |
title_short |
Avaliação de dois diferentes meios de cultura para o desenvolvimento de biopesticidas à base de Bacillus thuringiensis e sua aplicação em larvas de Aedes aegypti |
title_full |
Avaliação de dois diferentes meios de cultura para o desenvolvimento de biopesticidas à base de Bacillus thuringiensis e sua aplicação em larvas de Aedes aegypti |
title_fullStr |
Avaliação de dois diferentes meios de cultura para o desenvolvimento de biopesticidas à base de Bacillus thuringiensis e sua aplicação em larvas de Aedes aegypti |
title_full_unstemmed |
Avaliação de dois diferentes meios de cultura para o desenvolvimento de biopesticidas à base de Bacillus thuringiensis e sua aplicação em larvas de Aedes aegypti |
title_sort |
Avaliação de dois diferentes meios de cultura para o desenvolvimento de biopesticidas à base de Bacillus thuringiensis e sua aplicação em larvas de Aedes aegypti |
author |
Ernandes, Samara |
author_facet |
Ernandes, Samara Bianchi, Vanildo Luiz Del [UNESP] Oliveira Moraes, Iracema de |
author_role |
author |
author2 |
Bianchi, Vanildo Luiz Del [UNESP] Oliveira Moraes, Iracema de |
author2_role |
author author |
dc.contributor.none.fl_str_mv |
União das Faculdades dos Grandes Lagos (UNILAGO) Universidade Estadual Paulista (Unesp) P&D Experimental em Ciências Físicas e Naturais Ltda |
dc.contributor.author.fl_str_mv |
Ernandes, Samara Bianchi, Vanildo Luiz Del [UNESP] Oliveira Moraes, Iracema de |
dc.subject.por.fl_str_mv |
Agroindustrial waste Biological control Fermentation process |
topic |
Agroindustrial waste Biological control Fermentation process |
description |
The bacteria Bacillus thuringiensis var. israelensis (Bti) generates certain toxins with pesticide action, which can be used on the control of transmissible diseases by culicides, specially Aedes aegypti, the dengue vector. This biopesticide has been produced by submerged fermentation and, in Brazil, this production has been made by very little research centers and, more recently, by a unique small enterprise. For the implementation of a viable vectors control program through biopesticides, some studies about culture media are essential in order to join efficiency and low costs. In this way, agroindustrial wastes or by-products have been used as a nutrient source for the culture media production. In this study, corn steep liquor, a corn industrial processing by-product and tryptose, both with/without sugar addition, were compared as culture media. Cellular growth was evaluated by optical density at 620 nm, spore production by total viable cell count and LC50 by bioassays against 4th instar larvae. Among the four examined substrates, the medium composed by glucose plus corn steep liquor presented the best spore production and bioassay results. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-02-11 2014-05-27T11:28:24Z 2014-05-27T11:28:24Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.4025/actascitechnol.v35i1.13831 Acta Scientiarum - Technology, v. 35, n. 1, p. 11-18, 2013. 1806-2563 1807-8664 http://hdl.handle.net/11449/74584 10.4025/actascitechnol.v35i1.13831 WOS:000315355200003 2-s2.0-84873387692 2-s2.0-84873387692.pdf 9441894011582350 |
url |
http://dx.doi.org/10.4025/actascitechnol.v35i1.13831 http://hdl.handle.net/11449/74584 |
identifier_str_mv |
Acta Scientiarum - Technology, v. 35, n. 1, p. 11-18, 2013. 1806-2563 1807-8664 10.4025/actascitechnol.v35i1.13831 WOS:000315355200003 2-s2.0-84873387692 2-s2.0-84873387692.pdf 9441894011582350 |
dc.language.iso.fl_str_mv |
eng por |
language |
eng por |
dc.relation.none.fl_str_mv |
Acta Scientiarum: Technology 0.231 0,168 0,168 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
11-18 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1808129378957131776 |