Antimicrobial resistance profile of planktonic and biofilm cells of staphylococcus aureus and coagulase-negative staphylococci

Detalhes bibliográficos
Autor(a) principal: de Oliveira, Adilson [UNESP]
Data de Publicação: 2016
Outros Autores: Pereira, Valéria Cataneli [UNESP], Pinheiro, Luiza [UNESP], Riboli, Danilo Flávio Moraes [UNESP], Martins, Katheryne Benini [UNESP], de Lourdes Ribeiro de Souza da Cunha, Maria [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.3390/ijms17091423
http://hdl.handle.net/11449/173440
Resumo: The objective of the present study was to determine the antimicrobial resistance profile of planktonic and biofilm cells of Staphylococcus aureus and coagulase-negative staphylococci (CoNS). Two hundred Staphylococcus spp. strains were studied, including 50 S. aureus and 150 CoNS strains (50 S. epidermidis, 20 S. haemolyticus, 20 S. warneri, 20 S. hominis, 20 S. lugdunensis, and 20 S. saprophyticus). Biofilm formation was investigated by adherence to polystyrene plates. Positive strains were submitted to the broth microdilution method to determine the minimum inhibitory concentration (MIC) for planktonic and biofilm cells and the minimal bactericidal concentration for biofilm cells (MBCB). Forty-nine Staphylococcus spp. strains (14 S. aureus, 13 S. epidermidis, 13 S. saprophyticus, 3 S. haemolyticus, 1 S. hominis, 3 S. warneri, and 2 S. lugdunensis) were biofilm producers. These isolates were evaluated regarding their resistance profile. Determination of planktonic cell MIC identified three (21.4%) S. aureus strains that were resistant to oxacillin and six (42.8%) that were resistant to erythromycin. Among the CoNS, 31 (88.6%) strains were resistant to oxacillin, 14 (40%) to erythromycin, 18 (51.4%) to gentamicin, and 8 (22.8%) to sulfamethoxazole/trimethoprim. None of the planktonic isolates were resistant to vancomycin or linezolid. MICs were 2-, 4-, 8-, and up to 16-fold higher for biofilm cells than for planktonic cells. This observation was more common for vancomycin and erythromycin. The MBCB ranged from 8 to >256 μg/mL for oxacillin, 128 to >128 μg/mL for vancomycin, 256 to >256 μg/mL for erythromycin and gentamicin, >64 μg/mL for linezolid, and 32/608 to >32/608 μg/mL for sulfamethoxazole/trimethoprim. The results showed considerably higher MICs for S. aureus and CoNS biofilm cells compared to planktonic cells. Analysis of MBCM confirmed that even high concentrations of vancomycin were unable to eliminate the biofilms of S. aureus and CoNS species. Linezolid was the most effective drug in inhibiting staphylococci in the biofilm, without an increase in the MIC, when compared to planktonic cells. None of the isolates were resistant to this drug.
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spelling Antimicrobial resistance profile of planktonic and biofilm cells of staphylococcus aureus and coagulase-negative staphylococciAntimicrobialsBiofilmCoagulase-negative staphylococciResistanceStaphylococcus aureusThe objective of the present study was to determine the antimicrobial resistance profile of planktonic and biofilm cells of Staphylococcus aureus and coagulase-negative staphylococci (CoNS). Two hundred Staphylococcus spp. strains were studied, including 50 S. aureus and 150 CoNS strains (50 S. epidermidis, 20 S. haemolyticus, 20 S. warneri, 20 S. hominis, 20 S. lugdunensis, and 20 S. saprophyticus). Biofilm formation was investigated by adherence to polystyrene plates. Positive strains were submitted to the broth microdilution method to determine the minimum inhibitory concentration (MIC) for planktonic and biofilm cells and the minimal bactericidal concentration for biofilm cells (MBCB). Forty-nine Staphylococcus spp. strains (14 S. aureus, 13 S. epidermidis, 13 S. saprophyticus, 3 S. haemolyticus, 1 S. hominis, 3 S. warneri, and 2 S. lugdunensis) were biofilm producers. These isolates were evaluated regarding their resistance profile. Determination of planktonic cell MIC identified three (21.4%) S. aureus strains that were resistant to oxacillin and six (42.8%) that were resistant to erythromycin. Among the CoNS, 31 (88.6%) strains were resistant to oxacillin, 14 (40%) to erythromycin, 18 (51.4%) to gentamicin, and 8 (22.8%) to sulfamethoxazole/trimethoprim. None of the planktonic isolates were resistant to vancomycin or linezolid. MICs were 2-, 4-, 8-, and up to 16-fold higher for biofilm cells than for planktonic cells. This observation was more common for vancomycin and erythromycin. The MBCB ranged from 8 to >256 μg/mL for oxacillin, 128 to >128 μg/mL for vancomycin, 256 to >256 μg/mL for erythromycin and gentamicin, >64 μg/mL for linezolid, and 32/608 to >32/608 μg/mL for sulfamethoxazole/trimethoprim. The results showed considerably higher MICs for S. aureus and CoNS biofilm cells compared to planktonic cells. Analysis of MBCM confirmed that even high concentrations of vancomycin were unable to eliminate the biofilms of S. aureus and CoNS species. Linezolid was the most effective drug in inhibiting staphylococci in the biofilm, without an increase in the MIC, when compared to planktonic cells. None of the isolates were resistant to this drug.Departamento de Microbiologia e Imunologia Instituto de Biociências UNESP—Univ Estadual Paulista Rua Professor Doutor Antonio CelsoWagner Zanin, s/n, BotucatuDepartamento de Anatomia Patológica Instituto Lauro de Souza LimaDepartamento de Microbiologia e Imunologia Instituto de Biociências UNESP—Univ Estadual Paulista Rua Professor Doutor Antonio CelsoWagner Zanin, s/n, BotucatuUniversidade Estadual Paulista (Unesp)Instituto Lauro de Souza Limade Oliveira, Adilson [UNESP]Pereira, Valéria Cataneli [UNESP]Pinheiro, Luiza [UNESP]Riboli, Danilo Flávio Moraes [UNESP]Martins, Katheryne Benini [UNESP]de Lourdes Ribeiro de Souza da Cunha, Maria [UNESP]2018-12-11T17:05:29Z2018-12-11T17:05:29Z2016-09-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://dx.doi.org/10.3390/ijms17091423International Journal of Molecular Sciences, v. 17, n. 9, 2016.1422-00671661-6596http://hdl.handle.net/11449/17344010.3390/ijms170914232-s2.0-849859168762-s2.0-84985916876.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengInternational Journal of Molecular Sciences1,260info:eu-repo/semantics/openAccess2023-12-28T06:22:03Zoai:repositorio.unesp.br:11449/173440Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T21:32:58.296596Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Antimicrobial resistance profile of planktonic and biofilm cells of staphylococcus aureus and coagulase-negative staphylococci
title Antimicrobial resistance profile of planktonic and biofilm cells of staphylococcus aureus and coagulase-negative staphylococci
spellingShingle Antimicrobial resistance profile of planktonic and biofilm cells of staphylococcus aureus and coagulase-negative staphylococci
de Oliveira, Adilson [UNESP]
Antimicrobials
Biofilm
Coagulase-negative staphylococci
Resistance
Staphylococcus aureus
title_short Antimicrobial resistance profile of planktonic and biofilm cells of staphylococcus aureus and coagulase-negative staphylococci
title_full Antimicrobial resistance profile of planktonic and biofilm cells of staphylococcus aureus and coagulase-negative staphylococci
title_fullStr Antimicrobial resistance profile of planktonic and biofilm cells of staphylococcus aureus and coagulase-negative staphylococci
title_full_unstemmed Antimicrobial resistance profile of planktonic and biofilm cells of staphylococcus aureus and coagulase-negative staphylococci
title_sort Antimicrobial resistance profile of planktonic and biofilm cells of staphylococcus aureus and coagulase-negative staphylococci
author de Oliveira, Adilson [UNESP]
author_facet de Oliveira, Adilson [UNESP]
Pereira, Valéria Cataneli [UNESP]
Pinheiro, Luiza [UNESP]
Riboli, Danilo Flávio Moraes [UNESP]
Martins, Katheryne Benini [UNESP]
de Lourdes Ribeiro de Souza da Cunha, Maria [UNESP]
author_role author
author2 Pereira, Valéria Cataneli [UNESP]
Pinheiro, Luiza [UNESP]
Riboli, Danilo Flávio Moraes [UNESP]
Martins, Katheryne Benini [UNESP]
de Lourdes Ribeiro de Souza da Cunha, Maria [UNESP]
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Instituto Lauro de Souza Lima
dc.contributor.author.fl_str_mv de Oliveira, Adilson [UNESP]
Pereira, Valéria Cataneli [UNESP]
Pinheiro, Luiza [UNESP]
Riboli, Danilo Flávio Moraes [UNESP]
Martins, Katheryne Benini [UNESP]
de Lourdes Ribeiro de Souza da Cunha, Maria [UNESP]
dc.subject.por.fl_str_mv Antimicrobials
Biofilm
Coagulase-negative staphylococci
Resistance
Staphylococcus aureus
topic Antimicrobials
Biofilm
Coagulase-negative staphylococci
Resistance
Staphylococcus aureus
description The objective of the present study was to determine the antimicrobial resistance profile of planktonic and biofilm cells of Staphylococcus aureus and coagulase-negative staphylococci (CoNS). Two hundred Staphylococcus spp. strains were studied, including 50 S. aureus and 150 CoNS strains (50 S. epidermidis, 20 S. haemolyticus, 20 S. warneri, 20 S. hominis, 20 S. lugdunensis, and 20 S. saprophyticus). Biofilm formation was investigated by adherence to polystyrene plates. Positive strains were submitted to the broth microdilution method to determine the minimum inhibitory concentration (MIC) for planktonic and biofilm cells and the minimal bactericidal concentration for biofilm cells (MBCB). Forty-nine Staphylococcus spp. strains (14 S. aureus, 13 S. epidermidis, 13 S. saprophyticus, 3 S. haemolyticus, 1 S. hominis, 3 S. warneri, and 2 S. lugdunensis) were biofilm producers. These isolates were evaluated regarding their resistance profile. Determination of planktonic cell MIC identified three (21.4%) S. aureus strains that were resistant to oxacillin and six (42.8%) that were resistant to erythromycin. Among the CoNS, 31 (88.6%) strains were resistant to oxacillin, 14 (40%) to erythromycin, 18 (51.4%) to gentamicin, and 8 (22.8%) to sulfamethoxazole/trimethoprim. None of the planktonic isolates were resistant to vancomycin or linezolid. MICs were 2-, 4-, 8-, and up to 16-fold higher for biofilm cells than for planktonic cells. This observation was more common for vancomycin and erythromycin. The MBCB ranged from 8 to >256 μg/mL for oxacillin, 128 to >128 μg/mL for vancomycin, 256 to >256 μg/mL for erythromycin and gentamicin, >64 μg/mL for linezolid, and 32/608 to >32/608 μg/mL for sulfamethoxazole/trimethoprim. The results showed considerably higher MICs for S. aureus and CoNS biofilm cells compared to planktonic cells. Analysis of MBCM confirmed that even high concentrations of vancomycin were unable to eliminate the biofilms of S. aureus and CoNS species. Linezolid was the most effective drug in inhibiting staphylococci in the biofilm, without an increase in the MIC, when compared to planktonic cells. None of the isolates were resistant to this drug.
publishDate 2016
dc.date.none.fl_str_mv 2016-09-01
2018-12-11T17:05:29Z
2018-12-11T17:05:29Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.3390/ijms17091423
International Journal of Molecular Sciences, v. 17, n. 9, 2016.
1422-0067
1661-6596
http://hdl.handle.net/11449/173440
10.3390/ijms17091423
2-s2.0-84985916876
2-s2.0-84985916876.pdf
url http://dx.doi.org/10.3390/ijms17091423
http://hdl.handle.net/11449/173440
identifier_str_mv International Journal of Molecular Sciences, v. 17, n. 9, 2016.
1422-0067
1661-6596
10.3390/ijms17091423
2-s2.0-84985916876
2-s2.0-84985916876.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv International Journal of Molecular Sciences
1,260
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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