Generation of neural progenitor cells from porcine-induced pluripotent stem cells
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1002/term.3143 http://hdl.handle.net/11449/209677 |
Resumo: | In this study, porcine embryonic fibroblasts (pEFs) were reprogrammed into porcine-induced pluripotent stem cells (piPSCs) using either human or mouse specific sequences for the OCT4, SOX2, c-Myc, and KLF4 transcription factors. In total, three pEFs lines were reprogrammed, cultured for at least 15 passages, and characterized regarding their pluripotency status (alkaline phosphatase expression, embryoid body formation, expression of exogenous and endogenous genes, and immunofluorescence). Two piPSC lines were further differentiated, using chemical inhibitors, into putative neural progenitor-like (NPC-like) cells with subsequent analyses of their morphology and expression of neural markers such as NESTIN and GFAP as well as immunofluorescent labeling of NESTIN, beta-TUBULIN III, and VIMENTIN. NPC-like cells were positive for all the neural markers tested. These results evidence of the generation of porcine NPC-like cells after in vitro induction with chemical inhibitors, representing an adequate model for future regenerative and translational medicine research. |
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Repositório Institucional da UNESP |
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2946 |
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Generation of neural progenitor cells from porcine-induced pluripotent stem cellscellular reprogramminginduced pluripotent stem cells (iPSC)neural progenitor cellsswineIn this study, porcine embryonic fibroblasts (pEFs) were reprogrammed into porcine-induced pluripotent stem cells (piPSCs) using either human or mouse specific sequences for the OCT4, SOX2, c-Myc, and KLF4 transcription factors. In total, three pEFs lines were reprogrammed, cultured for at least 15 passages, and characterized regarding their pluripotency status (alkaline phosphatase expression, embryoid body formation, expression of exogenous and endogenous genes, and immunofluorescence). Two piPSC lines were further differentiated, using chemical inhibitors, into putative neural progenitor-like (NPC-like) cells with subsequent analyses of their morphology and expression of neural markers such as NESTIN and GFAP as well as immunofluorescent labeling of NESTIN, beta-TUBULIN III, and VIMENTIN. NPC-like cells were positive for all the neural markers tested. These results evidence of the generation of porcine NPC-like cells after in vitro induction with chemical inhibitors, representing an adequate model for future regenerative and translational medicine research.Coordination for the Improvement of Higher Education PersonnelNational Council for Scientific and Technological DevelopmentFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Univ Sao Paulo, Fac Anim Sci & Food Engn, Dept Vet Med, Pirassununga, BrazilUniv Sao Paulo, Fac Vet Med & Anim Sci, Dept Surg, Postgrad Program Anat Domest & Wild Anim, Sao Paulo, BrazilUniv Sao Paulo, Fac Vet Med & Anim Sci, Dept Anim Reprod, Sao Paulo, BrazilSao Paulo State Univ, Inst Biosci, Dept Pharmacol, Botucatu, SP, BrazilSao Paulo State Univ, Fac Agr & Vet Sci, Dept Prevent Vet Med & Anim Reprod, Jaboticabal, BrazilUniv Sao Paulo, Fac Anim Sci & Food Engn, Dept Anim Sci, Pirassununga, BrazilUniv Copenhagen, Dept Vet & Anim Sci, Copenhagen, DenmarkSao Paulo State Univ, Inst Biosci, Dept Pharmacol, Botucatu, SP, BrazilSao Paulo State Univ, Fac Agr & Vet Sci, Dept Prevent Vet Med & Anim Reprod, Jaboticabal, BrazilNational Council for Scientific and Technological Development: 433133/2018-0FAPESP: 2013/08135-2FAPESP: 2015/26818-5FAPESP: 2017/02159-8FAPESP: 2017/13973-8Wiley-BlackwellUniversidade de São Paulo (USP)Universidade Estadual Paulista (Unesp)Univ CopenhagenMachado, Lucas SimoesPieri, Naira Caroline GodoyBotigelli, Ramon Cesar [UNESP]Castro, Raquel Vasconcelos Guimaraes de [UNESP]Souza, Aline Fernanda deBridi, AlessandraLima, Marina AmaroFantinato Neto, PauloPessoa, Lais Vicari de FigueiredoMartins, Simone Maria Massami KitamuraDe Andrade, Andre Furugen CesarFreude, Kristine KarlaBressan, Fabiana Fernandes2021-06-25T12:25:42Z2021-06-25T12:25:42Z2020-11-23info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1880-1891http://dx.doi.org/10.1002/term.3143Journal Of Tissue Engineering And Regenerative Medicine. Hoboken: Wiley, v. 14, n. 12, p. 1880-1891, 2020.1932-6254http://hdl.handle.net/11449/20967710.1002/term.3143WOS:000591386700001Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal Of Tissue Engineering And Regenerative Medicineinfo:eu-repo/semantics/openAccess2024-06-06T18:09:33Zoai:repositorio.unesp.br:11449/209677Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T17:03:14.321757Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Generation of neural progenitor cells from porcine-induced pluripotent stem cells |
title |
Generation of neural progenitor cells from porcine-induced pluripotent stem cells |
spellingShingle |
Generation of neural progenitor cells from porcine-induced pluripotent stem cells Machado, Lucas Simoes cellular reprogramming induced pluripotent stem cells (iPSC) neural progenitor cells swine |
title_short |
Generation of neural progenitor cells from porcine-induced pluripotent stem cells |
title_full |
Generation of neural progenitor cells from porcine-induced pluripotent stem cells |
title_fullStr |
Generation of neural progenitor cells from porcine-induced pluripotent stem cells |
title_full_unstemmed |
Generation of neural progenitor cells from porcine-induced pluripotent stem cells |
title_sort |
Generation of neural progenitor cells from porcine-induced pluripotent stem cells |
author |
Machado, Lucas Simoes |
author_facet |
Machado, Lucas Simoes Pieri, Naira Caroline Godoy Botigelli, Ramon Cesar [UNESP] Castro, Raquel Vasconcelos Guimaraes de [UNESP] Souza, Aline Fernanda de Bridi, Alessandra Lima, Marina Amaro Fantinato Neto, Paulo Pessoa, Lais Vicari de Figueiredo Martins, Simone Maria Massami Kitamura De Andrade, Andre Furugen Cesar Freude, Kristine Karla Bressan, Fabiana Fernandes |
author_role |
author |
author2 |
Pieri, Naira Caroline Godoy Botigelli, Ramon Cesar [UNESP] Castro, Raquel Vasconcelos Guimaraes de [UNESP] Souza, Aline Fernanda de Bridi, Alessandra Lima, Marina Amaro Fantinato Neto, Paulo Pessoa, Lais Vicari de Figueiredo Martins, Simone Maria Massami Kitamura De Andrade, Andre Furugen Cesar Freude, Kristine Karla Bressan, Fabiana Fernandes |
author2_role |
author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) Universidade Estadual Paulista (Unesp) Univ Copenhagen |
dc.contributor.author.fl_str_mv |
Machado, Lucas Simoes Pieri, Naira Caroline Godoy Botigelli, Ramon Cesar [UNESP] Castro, Raquel Vasconcelos Guimaraes de [UNESP] Souza, Aline Fernanda de Bridi, Alessandra Lima, Marina Amaro Fantinato Neto, Paulo Pessoa, Lais Vicari de Figueiredo Martins, Simone Maria Massami Kitamura De Andrade, Andre Furugen Cesar Freude, Kristine Karla Bressan, Fabiana Fernandes |
dc.subject.por.fl_str_mv |
cellular reprogramming induced pluripotent stem cells (iPSC) neural progenitor cells swine |
topic |
cellular reprogramming induced pluripotent stem cells (iPSC) neural progenitor cells swine |
description |
In this study, porcine embryonic fibroblasts (pEFs) were reprogrammed into porcine-induced pluripotent stem cells (piPSCs) using either human or mouse specific sequences for the OCT4, SOX2, c-Myc, and KLF4 transcription factors. In total, three pEFs lines were reprogrammed, cultured for at least 15 passages, and characterized regarding their pluripotency status (alkaline phosphatase expression, embryoid body formation, expression of exogenous and endogenous genes, and immunofluorescence). Two piPSC lines were further differentiated, using chemical inhibitors, into putative neural progenitor-like (NPC-like) cells with subsequent analyses of their morphology and expression of neural markers such as NESTIN and GFAP as well as immunofluorescent labeling of NESTIN, beta-TUBULIN III, and VIMENTIN. NPC-like cells were positive for all the neural markers tested. These results evidence of the generation of porcine NPC-like cells after in vitro induction with chemical inhibitors, representing an adequate model for future regenerative and translational medicine research. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-11-23 2021-06-25T12:25:42Z 2021-06-25T12:25:42Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1002/term.3143 Journal Of Tissue Engineering And Regenerative Medicine. Hoboken: Wiley, v. 14, n. 12, p. 1880-1891, 2020. 1932-6254 http://hdl.handle.net/11449/209677 10.1002/term.3143 WOS:000591386700001 |
url |
http://dx.doi.org/10.1002/term.3143 http://hdl.handle.net/11449/209677 |
identifier_str_mv |
Journal Of Tissue Engineering And Regenerative Medicine. Hoboken: Wiley, v. 14, n. 12, p. 1880-1891, 2020. 1932-6254 10.1002/term.3143 WOS:000591386700001 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal Of Tissue Engineering And Regenerative Medicine |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1880-1891 |
dc.publisher.none.fl_str_mv |
Wiley-Blackwell |
publisher.none.fl_str_mv |
Wiley-Blackwell |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128745829040128 |