Generation of neural progenitor cells from porcine-induced pluripotent stem cells

Detalhes bibliográficos
Autor(a) principal: Machado, Lucas Simoes
Data de Publicação: 2020
Outros Autores: Pieri, Naira Caroline Godoy, Botigelli, Ramon Cesar [UNESP], Castro, Raquel Vasconcelos Guimaraes de [UNESP], Souza, Aline Fernanda de, Bridi, Alessandra, Lima, Marina Amaro, Fantinato Neto, Paulo, Pessoa, Lais Vicari de Figueiredo, Martins, Simone Maria Massami Kitamura, De Andrade, Andre Furugen Cesar, Freude, Kristine Karla, Bressan, Fabiana Fernandes
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1002/term.3143
http://hdl.handle.net/11449/209677
Resumo: In this study, porcine embryonic fibroblasts (pEFs) were reprogrammed into porcine-induced pluripotent stem cells (piPSCs) using either human or mouse specific sequences for the OCT4, SOX2, c-Myc, and KLF4 transcription factors. In total, three pEFs lines were reprogrammed, cultured for at least 15 passages, and characterized regarding their pluripotency status (alkaline phosphatase expression, embryoid body formation, expression of exogenous and endogenous genes, and immunofluorescence). Two piPSC lines were further differentiated, using chemical inhibitors, into putative neural progenitor-like (NPC-like) cells with subsequent analyses of their morphology and expression of neural markers such as NESTIN and GFAP as well as immunofluorescent labeling of NESTIN, beta-TUBULIN III, and VIMENTIN. NPC-like cells were positive for all the neural markers tested. These results evidence of the generation of porcine NPC-like cells after in vitro induction with chemical inhibitors, representing an adequate model for future regenerative and translational medicine research.
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spelling Generation of neural progenitor cells from porcine-induced pluripotent stem cellscellular reprogramminginduced pluripotent stem cells (iPSC)neural progenitor cellsswineIn this study, porcine embryonic fibroblasts (pEFs) were reprogrammed into porcine-induced pluripotent stem cells (piPSCs) using either human or mouse specific sequences for the OCT4, SOX2, c-Myc, and KLF4 transcription factors. In total, three pEFs lines were reprogrammed, cultured for at least 15 passages, and characterized regarding their pluripotency status (alkaline phosphatase expression, embryoid body formation, expression of exogenous and endogenous genes, and immunofluorescence). Two piPSC lines were further differentiated, using chemical inhibitors, into putative neural progenitor-like (NPC-like) cells with subsequent analyses of their morphology and expression of neural markers such as NESTIN and GFAP as well as immunofluorescent labeling of NESTIN, beta-TUBULIN III, and VIMENTIN. NPC-like cells were positive for all the neural markers tested. These results evidence of the generation of porcine NPC-like cells after in vitro induction with chemical inhibitors, representing an adequate model for future regenerative and translational medicine research.Coordination for the Improvement of Higher Education PersonnelNational Council for Scientific and Technological DevelopmentFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Univ Sao Paulo, Fac Anim Sci & Food Engn, Dept Vet Med, Pirassununga, BrazilUniv Sao Paulo, Fac Vet Med & Anim Sci, Dept Surg, Postgrad Program Anat Domest & Wild Anim, Sao Paulo, BrazilUniv Sao Paulo, Fac Vet Med & Anim Sci, Dept Anim Reprod, Sao Paulo, BrazilSao Paulo State Univ, Inst Biosci, Dept Pharmacol, Botucatu, SP, BrazilSao Paulo State Univ, Fac Agr & Vet Sci, Dept Prevent Vet Med & Anim Reprod, Jaboticabal, BrazilUniv Sao Paulo, Fac Anim Sci & Food Engn, Dept Anim Sci, Pirassununga, BrazilUniv Copenhagen, Dept Vet & Anim Sci, Copenhagen, DenmarkSao Paulo State Univ, Inst Biosci, Dept Pharmacol, Botucatu, SP, BrazilSao Paulo State Univ, Fac Agr & Vet Sci, Dept Prevent Vet Med & Anim Reprod, Jaboticabal, BrazilNational Council for Scientific and Technological Development: 433133/2018-0FAPESP: 2013/08135-2FAPESP: 2015/26818-5FAPESP: 2017/02159-8FAPESP: 2017/13973-8Wiley-BlackwellUniversidade de São Paulo (USP)Universidade Estadual Paulista (Unesp)Univ CopenhagenMachado, Lucas SimoesPieri, Naira Caroline GodoyBotigelli, Ramon Cesar [UNESP]Castro, Raquel Vasconcelos Guimaraes de [UNESP]Souza, Aline Fernanda deBridi, AlessandraLima, Marina AmaroFantinato Neto, PauloPessoa, Lais Vicari de FigueiredoMartins, Simone Maria Massami KitamuraDe Andrade, Andre Furugen CesarFreude, Kristine KarlaBressan, Fabiana Fernandes2021-06-25T12:25:42Z2021-06-25T12:25:42Z2020-11-23info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1880-1891http://dx.doi.org/10.1002/term.3143Journal Of Tissue Engineering And Regenerative Medicine. Hoboken: Wiley, v. 14, n. 12, p. 1880-1891, 2020.1932-6254http://hdl.handle.net/11449/20967710.1002/term.3143WOS:000591386700001Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal Of Tissue Engineering And Regenerative Medicineinfo:eu-repo/semantics/openAccess2024-06-06T18:09:33Zoai:repositorio.unesp.br:11449/209677Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T17:03:14.321757Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Generation of neural progenitor cells from porcine-induced pluripotent stem cells
title Generation of neural progenitor cells from porcine-induced pluripotent stem cells
spellingShingle Generation of neural progenitor cells from porcine-induced pluripotent stem cells
Machado, Lucas Simoes
cellular reprogramming
induced pluripotent stem cells (iPSC)
neural progenitor cells
swine
title_short Generation of neural progenitor cells from porcine-induced pluripotent stem cells
title_full Generation of neural progenitor cells from porcine-induced pluripotent stem cells
title_fullStr Generation of neural progenitor cells from porcine-induced pluripotent stem cells
title_full_unstemmed Generation of neural progenitor cells from porcine-induced pluripotent stem cells
title_sort Generation of neural progenitor cells from porcine-induced pluripotent stem cells
author Machado, Lucas Simoes
author_facet Machado, Lucas Simoes
Pieri, Naira Caroline Godoy
Botigelli, Ramon Cesar [UNESP]
Castro, Raquel Vasconcelos Guimaraes de [UNESP]
Souza, Aline Fernanda de
Bridi, Alessandra
Lima, Marina Amaro
Fantinato Neto, Paulo
Pessoa, Lais Vicari de Figueiredo
Martins, Simone Maria Massami Kitamura
De Andrade, Andre Furugen Cesar
Freude, Kristine Karla
Bressan, Fabiana Fernandes
author_role author
author2 Pieri, Naira Caroline Godoy
Botigelli, Ramon Cesar [UNESP]
Castro, Raquel Vasconcelos Guimaraes de [UNESP]
Souza, Aline Fernanda de
Bridi, Alessandra
Lima, Marina Amaro
Fantinato Neto, Paulo
Pessoa, Lais Vicari de Figueiredo
Martins, Simone Maria Massami Kitamura
De Andrade, Andre Furugen Cesar
Freude, Kristine Karla
Bressan, Fabiana Fernandes
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade de São Paulo (USP)
Universidade Estadual Paulista (Unesp)
Univ Copenhagen
dc.contributor.author.fl_str_mv Machado, Lucas Simoes
Pieri, Naira Caroline Godoy
Botigelli, Ramon Cesar [UNESP]
Castro, Raquel Vasconcelos Guimaraes de [UNESP]
Souza, Aline Fernanda de
Bridi, Alessandra
Lima, Marina Amaro
Fantinato Neto, Paulo
Pessoa, Lais Vicari de Figueiredo
Martins, Simone Maria Massami Kitamura
De Andrade, Andre Furugen Cesar
Freude, Kristine Karla
Bressan, Fabiana Fernandes
dc.subject.por.fl_str_mv cellular reprogramming
induced pluripotent stem cells (iPSC)
neural progenitor cells
swine
topic cellular reprogramming
induced pluripotent stem cells (iPSC)
neural progenitor cells
swine
description In this study, porcine embryonic fibroblasts (pEFs) were reprogrammed into porcine-induced pluripotent stem cells (piPSCs) using either human or mouse specific sequences for the OCT4, SOX2, c-Myc, and KLF4 transcription factors. In total, three pEFs lines were reprogrammed, cultured for at least 15 passages, and characterized regarding their pluripotency status (alkaline phosphatase expression, embryoid body formation, expression of exogenous and endogenous genes, and immunofluorescence). Two piPSC lines were further differentiated, using chemical inhibitors, into putative neural progenitor-like (NPC-like) cells with subsequent analyses of their morphology and expression of neural markers such as NESTIN and GFAP as well as immunofluorescent labeling of NESTIN, beta-TUBULIN III, and VIMENTIN. NPC-like cells were positive for all the neural markers tested. These results evidence of the generation of porcine NPC-like cells after in vitro induction with chemical inhibitors, representing an adequate model for future regenerative and translational medicine research.
publishDate 2020
dc.date.none.fl_str_mv 2020-11-23
2021-06-25T12:25:42Z
2021-06-25T12:25:42Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1002/term.3143
Journal Of Tissue Engineering And Regenerative Medicine. Hoboken: Wiley, v. 14, n. 12, p. 1880-1891, 2020.
1932-6254
http://hdl.handle.net/11449/209677
10.1002/term.3143
WOS:000591386700001
url http://dx.doi.org/10.1002/term.3143
http://hdl.handle.net/11449/209677
identifier_str_mv Journal Of Tissue Engineering And Regenerative Medicine. Hoboken: Wiley, v. 14, n. 12, p. 1880-1891, 2020.
1932-6254
10.1002/term.3143
WOS:000591386700001
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Journal Of Tissue Engineering And Regenerative Medicine
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 1880-1891
dc.publisher.none.fl_str_mv Wiley-Blackwell
publisher.none.fl_str_mv Wiley-Blackwell
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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